A2AQ25 (SKT_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 45.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Sickle tail protein Alternative name(s): Enhancer trap locus 4 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus![]() |
Protein attributes
| Sequence length | 1946 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Required for normal development of intervertebral disks. Ref.1 |
| Subcellular location | |
| Tissue specificity | Expressed predominantly in the notochord and mesonephros during embryogenesis as well as in other areas such as the epithalamus sulcus, lens vesicle, inner retinal layer, heart, hepatic primordial surface, infundibulum, surface ectoderm, hind gut and limb bud mesenchyme. In adults, expressed in a range of tissues including the nucleus pulposus, corpus callosum, kidney, cardiac muscle, Sertoli cells and hair follicles. Ref.1 |
| Disruption phenotype | Mice display a kinky-tail phenotype in about half of homozygotes with defects in the nucleus pulposus and annulus fibrosus of intertebral disks. Shortening and curving of caudal vertebrae 20-25 is apparent by the age of 2 weeks. Ref.1 |
| Sequence caution | The sequence AAH26657.2 differs from that shown. Reason: Erroneous initiation. The sequence BAC41473.2 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Coding sequence diversity | Alternative splicing |
| Domain | Coiled coil |
| Molecular function | Developmental protein |
| PTM | Glycoprotein Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | embryonic skeletal system development Inferred from mutant phenotype Ref.1. Source: UniProtKB |
| Cellular_component | cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB |
| Complete GO annotation... | |
Alternative products
| This entry describes 9 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 Ref.4 (identifier: A2AQ25-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: Gene prediction based on EST data. | ||||||
| Isoform 2 Ref.4 (identifier: A2AQ25-2) The sequence of this isoform differs from the canonical sequence as follows: 1-282: Missing. 560-594: Missing. | ||||||
| Note: Gene prediction based on EST data. | ||||||
| Isoform 3 Ref.1 (identifier: A2AQ25-3) Also known as: Skt-a; The sequence of this isoform differs from the canonical sequence as follows: 560-594: Missing. 1181-1739: Missing. | ||||||
| Isoform 4 Ref.1 (identifier: A2AQ25-4) Also known as: Skt-b; The sequence of this isoform differs from the canonical sequence as follows: 560-594: Missing. 961-971: Missing. 1181-1739: Missing. | ||||||
| Isoform 5 Ref.4 (identifier: A2AQ25-5) The sequence of this isoform differs from the canonical sequence as follows: 1-282: Missing. 560-594: Missing. 1207-1739: Missing. 1781-1784: ANGS → VVLP 1785-1946: Missing. | ||||||
| Note: Gene prediction based on EST data. | ||||||
| Isoform 6 Ref.4 (identifier: A2AQ25-6) The sequence of this isoform differs from the canonical sequence as follows: 1-282: Missing. 560-594: Missing. 1181-1739: Missing. 1781-1784: ANGS → VVLP 1785-1946: Missing. | ||||||
| Note: Gene prediction based on EST data. | ||||||
| Isoform 7 Ref.3 Ref.5 (identifier: A2AQ25-7) The sequence of this isoform differs from the canonical sequence as follows: 252-1946: Missing. | ||||||
| Isoform 8 Ref.2 (identifier: A2AQ25-8) The sequence of this isoform differs from the canonical sequence as follows: 560-594: Missing. 885-1755: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 9 Ref.3 (identifier: A2AQ25-9) The sequence of this isoform differs from the canonical sequence as follows: 1-282: Missing. 560-594: Missing. 961-971: Missing. 1181-1191: NLEFYHEDVRK → VTCGSYTFTIQ 1192-1946: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1946 | 1946 | Sickle tail protein | PRO_0000287898 | |||||
Regions | |||||||||
| Coiled coil | 557 – 581 | 25 | Potential | ||||||
| Coiled coil | 644 – 685 | 42 | Potential | ||||||
| Coiled coil | 962 – 990 | 29 | Potential | ||||||
| Coiled coil | 1469 – 1495 | 27 | Potential | ||||||
| Coiled coil | 1659 – 1688 | 30 | Potential | ||||||
| Compositional bias | 298 – 364 | 67 | Pro-rich | ||||||
| Compositional bias | 1050 – 1120 | 71 | Pro-rich | ||||||
| Compositional bias | 1796 – 1908 | 113 | Ser-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 244 | 1 | Phosphotyrosine Ref.7 | ||||||
| Modified residue | 245 | 1 | Phosphotyrosine Ref.7 | ||||||
| Modified residue | 359 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 361 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 393 | 1 | Phosphotyrosine By similarity UniProtKB Q5T5P2 | ||||||
| Modified residue | 474 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 1049 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 1899 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 1902 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 1905 | 1 | Phosphoserine Ref.8 Ref.9 | ||||||
| Glycosylation | 357 | 1 | O-linked (GlcNAc) Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 282 | 282 | Missing in isoform 2, isoform 5, isoform 6 and isoform 9. Ref.3 Ref.4 | VSP_052416 | |||||
| Alternative sequence | 252 – 1946 | 1695 | Missing in isoform 7. Ref.3 Ref.5 | VSP_052417 | |||||
| Alternative sequence | 560 – 594 | 35 | Missing in isoform 2, isoform 3, isoform 4, isoform 5, isoform 6, isoform 8 and isoform 9. Ref.1 Ref.2 Ref.3 Ref.4 | VSP_052418 | |||||
| Alternative sequence | 885 – 1755 | 871 | Missing in isoform 8. Ref.2 | VSP_052419 | |||||
| Alternative sequence | 961 – 971 | 11 | Missing in isoform 4 and isoform 9. Ref.1 Ref.3 | VSP_052420 | |||||
| Alternative sequence | 1181 – 1739 | 559 | Missing in isoform 3, isoform 4 and isoform 6. Ref.1 Ref.4 | VSP_052421 | |||||
| Alternative sequence | 1181 – 1191 | 11 | NLEFYHEDVRK → VTCGSYTFTIQ in isoform 9. Ref.3 | VSP_052422 | |||||
| Alternative sequence | 1192 – 1946 | 755 | Missing in isoform 9. Ref.3 | VSP_052423 | |||||
| Alternative sequence | 1207 – 1739 | 533 | Missing in isoform 5. Ref.4 | VSP_052424 | |||||
| Alternative sequence | 1781 – 1784 | 4 | ANGS → VVLP in isoform 5 and isoform 6. Ref.4 | VSP_052425 | |||||
| Alternative sequence | 1785 – 1946 | 162 | Missing in isoform 5 and isoform 6. Ref.4 | VSP_052426 | |||||
Experimental info | |||||||||
| Sequence conflict | 41 | 1 | R → H in AAH50016. Ref.5 | ||||||
| Sequence conflict | 119 | 1 | T → R in BAC41473. Ref.2 | ||||||
| Sequence conflict | 551 | 1 | T → A in BAC41473. Ref.2 | ||||||
| Sequence conflict | 818 | 1 | R → Q in BAC41473. Ref.2 | ||||||
Sequences
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "A novel murine gene, Sickle tail, linked to the Danforth's short tail locus, is required for normal development of the intervertebral disc." Semba K., Araki K., Li Z., Matsumoto K., Suzuki M., Nakagata N., Takagi K., Takeya M., Yoshinobu K., Araki M., Imai K., Abe K., Yamamura K. Genetics 172:445-456(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. |
| [2] | "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H. DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 8). Tissue: Embryonic tail. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 7 AND 9). Strain: C57BL/6J. Tissue: Kidney and Ovary. |
| [4] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 7), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 874-1946 (ISOFORM 3). Strain: 129/Sv X 129SvCp and FVB/N. Tissue: Colon and Embryonic stem cell. |
| [6] | "O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry." Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT SER-357, MASS SPECTROMETRY. Tissue: Brain. |
| [7] | "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-244 AND TYR-245, MASS SPECTROMETRY. Tissue: Brain. |
| [8] | "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1049; SER-1899 AND SER-1905, MASS SPECTROMETRY. Tissue: Brain cortex. |
| [9] | "Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474; SER-1902 AND SER-1905, MASS SPECTROMETRY. Tissue: Liver. |
| [10] | "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359 AND SER-361, MASS SPECTROMETRY. Tissue: Melanoma. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB125594 mRNA. Translation: BAD14929.1. AB125595 mRNA. Translation: BAD14930.1. AB093289 mRNA. Translation: BAC41473.2. Different initiation. AK052733 mRNA. Translation: BAC35121.1. AK054453 mRNA. Translation: BAC35783.1. AL844538, AL929100 Genomic DNA. Translation: CAM19813.1. AL844538, AL929100 Genomic DNA. Translation: CAM19814.1. AL844538, AL929100 Genomic DNA. Translation: CAM19815.1. AL929100, AL844538 Genomic DNA. Translation: CAM24302.1. AL929100, AL844538 Genomic DNA. Translation: CAM24303.1. AL929100, AL844538 Genomic DNA. Translation: CAM24304.1. AL929100 Genomic DNA. Translation: CAM24306.1. AL929100 Genomic DNA. Translation: CAM24307.1. AL929100 Genomic DNA. Translation: CAM24308.1. BC026657 mRNA. Translation: AAH26657.2. Different initiation. BC050016 mRNA. Translation: AAH50016.1. |
| IPI | IPI00411164. IPI00553590. IPI00752398. IPI00754456. IPI00756087. IPI00849799. IPI00849959. IPI00851081. IPI00968353. |
| RefSeq | NP_001074475.1. NM_001081006.1. NP_001171101.2. NM_001177630.2. NP_084171.2. NM_029895.4. NP_835160.2. NM_178059.5. |
| UniGene | Mm.237935. |
3D structure databases | |
| ProteinModelPortal | A2AQ25. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | A2AQ25. |
Proteomic databases | |
| PaxDb | A2AQ25. |
| PRIDE | A2AQ25. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000045555; ENSMUSP00000041431; ENSMUSG00000036617. ENSMUST00000066509; ENSMUSP00000066170; ENSMUSG00000036617. ENSMUST00000114606; ENSMUSP00000110253; ENSMUSG00000036617. ENSMUST00000114607; ENSMUSP00000110254; ENSMUSG00000036617. ENSMUST00000114608; ENSMUSP00000110255; ENSMUSG00000036617. ENSMUST00000114614; ENSMUSP00000110261; ENSMUSG00000036617. |
| GeneID | 208618. |
| KEGG | mmu:208618. |
| UCSC | uc008imn.1. mouse. uc008imo.1. mouse. uc008imp.1. mouse. uc008imq.1. mouse. uc008ims.1. mouse. uc008imt.1. mouse. |
Organism-specific databases | |
| CTD | 208618. |
| MGI | MGI:95454. Etl4. |
| Rouge | Search... |
Phylogenomic databases | |
| eggNOG | NOG72706. |
| GeneTree | ENSGT00390000012399. |
| HOVERGEN | HBG019587. |
Gene expression databases | |
| ArrayExpress | A2AQ25. |
| Bgee | A2AQ25. |
| CleanEx | MM_ETL4. |
| Genevestigator | A2AQ25. |
Family and domain databases | |
| InterPro | IPR026725. Skt. [Graphical view] |
| PANTHER | PTHR22741:SF4. PTHR22741:SF4. 1 hit. |
| ProtoNet | Search... |
Other | |
| ChiTaRS | Etl4. mouse. |
| NextBio | 372333. |
| SOURCE | Search... |
Entry information
| Entry name | SKT_MOUSE | ||||||||
| Accession | Primary (citable) accession number: A2AQ25 Secondary accession number(s): A2AUE9 Q8R0K6 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |

Clusters with
