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Protein

Protein Mis18-beta

Gene

Oip5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74ZincPROSITE-ProRule annotation1
Metal bindingi77ZincPROSITE-ProRule annotation1
Metal bindingi131ZincPROSITE-ProRule annotation1
Metal bindingi134ZincPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Mis18-beta
Alternative name(s):
Opa-interacting protein 5 homolog
Gene namesi
Name:Oip5
Synonyms:Ms18b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1917895. Oip5.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity
  • Chromosomecentromere By similarity

  • Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.By similarity

GO - Cellular componenti

  • Cajal body Source: MGI
  • chromatin Source: MGI
  • chromocenter Source: MGI
  • chromosome, centromeric region Source: GO_Central
  • cytosol Source: MGI
  • nuclear speck Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003598831 – 241Protein Mis18-betaAdd BLAST241

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphothreonineBy similarity1
Modified residuei42PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA2AQ14.
PaxDbiA2AQ14.
PRIDEiA2AQ14.

PTM databases

iPTMnetiA2AQ14.
PhosphoSitePlusiA2AQ14.

Expressioni

Gene expression databases

BgeeiENSMUSG00000072980.
GenevisibleiA2AQ14. MM.

Interactioni

Subunit structurei

Homodimer, and heterodimer with MIS18A. Identified in a complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000115219.

Structurei

3D structure databases

SMRiA2AQ14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 168Mis18PROSITE-ProRule annotationAdd BLAST100

Sequence similaritiesi

Belongs to the mis18 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IZ6R. Eukaryota.
ENOG410YQ0W. LUCA.
GeneTreeiENSGT00390000007347.
HOGENOMiHOG000113655.
HOVERGENiHBG095583.
InParanoidiA2AQ14.
KOiK11565.
OMAiFTTSMEW.
OrthoDBiEOG091G0KVE.
PhylomeDBiA2AQ14.
TreeFamiTF330739.

Family and domain databases

InterProiView protein in InterPro
IPR034752. Mis18.
IPR004910. Yippee/Mis18/Cereblon.
PfamiView protein in Pfam
PF03226. Yippee-Mis18. 1 hit.
PROSITEiView protein in PROSITE
PS51793. MIS18. 1 hit.

Sequencei

Sequence statusi: Complete.

A2AQ14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLSRRSLR FLETQSRRDS CDTPFSLTSS MEWDTQVVEG FSPLGSSESK
60 70 80 90 100
VKASPVDLRL PAWLEPERCA VFHCARCYAV LGDTLHLAWD LSRSLGALAF
110 120 130 140 150
SKVTNNVVLL EPFLVGIEGF LKSSTYNLLF CNSCGTPVGF HLYSTHAAMA
160 170 180 190 200
ALRGHFCLSS DKMLCYLLKT NAIVNTSEMD FHNVPLPEKI AELKEKIMLM
210 220 230 240
HTRLNSLTGL LKGKSPHQFK QENQQARKQH ILGLTASPKI L
Length:241
Mass (Da):26,861
Last modified:February 20, 2007 - v1
Checksum:i63AC2693958E29F4
GO

Sequence cautioni

The sequence AAI16661 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17R → H in AAI15884 (PubMed:15489334).Curated1
Sequence conflicti17R → H in AAI16661 (PubMed:15489334).Curated1
Sequence conflicti45G → V in AAI16661 (PubMed:15489334).Curated1
Sequence conflicti219F → S in AAI15884 (PubMed:15489334).Curated1
Sequence conflicti219F → S in AAI16661 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844536 Genomic DNA. Translation: CAM18072.1.
BC115883 mRNA. Translation: AAI15884.1.
BC116660 mRNA. Translation: AAI16661.1. Different initiation.
CCDSiCCDS38205.1.
RefSeqiNP_001036118.1. NM_001042653.1.
UniGeneiMm.246412.

Genome annotation databases

EnsembliENSMUST00000123818; ENSMUSP00000115219; ENSMUSG00000072980.
GeneIDi70645.
KEGGimmu:70645.
UCSCiuc008ltz.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMS18B_MOUSE
AccessioniPrimary (citable) accession number: A2AQ14
Secondary accession number(s): Q14AW4, Q14BH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: February 20, 2007
Last modified: June 7, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families