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A2APB8 (TPX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Targeting protein for Xklp2
Gene names
Name:Tpx2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length745 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation and protects the phosphorylated residue against dephosphorylation By similarity. Ref.5

Subunit structure

Interacts with AURKA. Ref.5

Subcellular location

Nucleus By similarity. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules By similarity. Ref.5

Sequence similarities

Belongs to the TPX2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 745745Targeting protein for Xklp2
PRO_0000393112

Amino acid modifications

Modified residue201Phosphoserine By similarity
Modified residue211Phosphoserine By similarity
Modified residue281Phosphothreonine By similarity
Modified residue331Phosphoserine By similarity
Modified residue721Phosphothreonine By similarity
Modified residue941Phosphoserine By similarity
Modified residue1131Phosphothreonine By similarity
Modified residue1211Phosphoserine By similarity
Modified residue1251Phosphoserine By similarity
Modified residue2111Phosphoserine By similarity
Modified residue2521Phosphoserine By similarity
Modified residue3071N6-acetyllysine By similarity
Modified residue3121Phosphoserine By similarity
Modified residue3401Phosphothreonine By similarity
Modified residue3591Phosphoserine By similarity
Modified residue3691Phosphothreonine By similarity
Modified residue3741Phosphothreonine By similarity
Modified residue4861Phosphoserine By similarity
Modified residue6511Phosphoserine By similarity
Modified residue6531Phosphoserine By similarity
Modified residue7371Phosphoserine Ref.6

Experimental info

Mutagenesis8 – 114YSFD → ASAA: Abolishes interaction with AURKA. Ref.5
Sequence conflict1651H → D in AAH60619. Ref.4
Sequence conflict4531E → K in BAE32280. Ref.1
Sequence conflict5091V → L in BAE32280. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A2APB8 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 4E60692BC52DAE34

FASTA74585,894
        10         20         30         40         50         60 
MSQVPTTYSF DAPTDFINFS SLDAEEDTEN IDSWFDEKAN LENKFLRQRG IGEPFQGKNS 

        70         80         90        100        110        120 
LRKAKLQQGF VTPLKAVDNT YHKETEKENL QKQSIPSNDC SSLDAKRAVS GNTPVQPQRR 

       130        140        150        160        170        180 
SIRLSAQKDL EQKEKNHVAS VEMKAKRCVA PATDCPPQKR MKVSHKKKLE EEEEGSAPAT 

       190        200        210        220        230        240 
SRKNERETLE KAKGKHTVPG VPPAREKVLK STEEQEIEKR LRMQQEVVEL RRKNEEFKKL 

       250        260        270        280        290        300 
ALAGPGQPVK KSTSQVTKTV DFHFLTDERI KQHPKNQEEY KEVNFMSELR KHSSTPARGT 

       310        320        330        340        350        360 
RGCTIIKPFN LSKGKKRTFD EAASTYVPIA QQVEAFHKRT PNRYHLRNKK DESLLPSKSV 

       370        380        390        400        410        420 
NKIARDPQTP ILQTKYRTRA VTCKSTAEQE AEELEKLQQY KFKARELDPR IFESGPILPK 

       430        440        450        460        470        480 
RAPVKPPTQP VGFDLEIEKR IHERESKKKT EDEQFEFHSR PCPTKILEDV VGVPEKKVIP 

       490        500        510        520        530        540 
ATVPKSPVFA LKNRIRVPIK DEEEEKPVVI KAQPVPHYGV PYKPHIAEAR NVEVCPFSFD 

       550        560        570        580        590        600 
TRDKERQLQK EKKIKEMQKG EVPKFKALPV PHFDTINLPE KKVKNVTQAE PFSLETDKRG 

       610        620        630        640        650        660 
AYKAEMWKHQ LEEEQKQQKD AACFKARPNT VIFQEPFVPK KEKKSLAENP SGSLVQEPFQ 

       670        680        690        700        710        720 
LATERRAKER QELEKKMAEV EAWKLQQLEE VRQQEEEQQK EELARLRKEL VHKANPIRKY 

       730        740 
AAVEVKSSEL PLTVPVSPKF STRFQ

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]"Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A."
Bird A.W., Hyman A.A.
J. Cell Biol. 182:289-300(2008) [PubMed: 18663142] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AURKA, SUBCELLULAR LOCATION, MUTAGENESIS OF 8-TYR--ASP-11.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, MASS SPECTROMETRY.
Tissue: Macrophage.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK153957 mRNA. Translation: BAE32280.1.
AL833801 Genomic DNA. Translation: CAM23677.1.
CH466551 Genomic DNA. Translation: EDL05994.1.
BC060619 mRNA. Translation: AAH60619.1.
IPIIPI00420481.
RefSeqNP_001135447.1. NM_001141975.1.
NP_001135448.1. NM_001141976.1.
NP_001135449.1. NM_001141977.1.
NP_001135450.1. NM_001141978.1.
NP_082385.3. NM_028109.4.
UniGeneMm.407737.

3D structure databases

ProteinModelPortalA2APB8.
SMRA2APB8. Positions 6-42.
ModBaseSearch...

Protein-protein interaction databases

STRINGA2APB8.

Proteomic databases

PRIDEA2APB8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028969; ENSMUSP00000028969; ENSMUSG00000027469.
ENSMUST00000109816; ENSMUSP00000105441; ENSMUSG00000027469.
ENSMUST00000164120; ENSMUSP00000128888; ENSMUSG00000027469.
GeneID72119.
KEGGmmu:72119.
UCSCuc008ngo.2. mouse.

Organism-specific databases

CTD22974.
MGIMGI:1919369. Tpx2.

Phylogenomic databases

eggNOGroNOG08761.
HOGENOMHBG445540.
HOVERGENHBG057334.
InParanoidA2APB8.
OMADERIKQH.
OrthoDBEOG434W5K.
PhylomeDBA2APB8.

Gene expression databases

BgeeA2APB8.
GenevestigatorA2APB8.

Family and domain databases

InterProIPR015128. Aurora-A-bd.
IPR022021. TPX2_importin.
IPR009675. Xklp2_targeting_prot.
[Graphical view]
PfamPF09041. Aurora-A_bind. 1 hit.
PF06886. TPX2. 1 hit.
PF12214. TPX2_importin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio335484.
SOURCESearch...

Entry information

Entry nameTPX2_MOUSE
AccessionPrimary (citable) accession number: A2APB8
Secondary accession number(s): Q3U500, Q6P9S6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 20, 2007
Last modified: September 21, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents