Reviewed, UniProtKB/Swiss-Prot A2AP18 (PLCH2_MOUSE)

Names and origin

Protein names

Recommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase eta-2
    EC=3.1.4.11
Alternative name(s):
    Phosphoinositide phospholipase C-eta-2
    Phospholipase C-eta-2
      Short name=PLC-eta2
    Phosphoinositide phospholipase C-like 4
      Short name=Phospholipase C-like protein 4
      Short name=PLC-L4

Gene names

Name:	Plch2
Synonyms:	Plcl4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain. Ref.1 Ref.4
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Subcellular location	Cytoplasm. Cell membrane. Note: Localized predominantly at the plasma membrane. Ref.4
Tissue specificity	Specifically detected in the brain, with higher level in cerebral cortex, olfactory bulb and hippocampus (at protein level). Expressed in the pyramidal cells of the hippocampus, but also in eye and lung. Ref.1 Ref.4
Developmental stage	Expressed at 2 weeks after birth but barely detected 1 week after birth. Increased expression during brain development. Ref.4
Sequence similarities	Contains 1 C2 domain. Contains 2 EF-hand domains. Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.
Sequence caution	The sequence BAC32005.1 differs from that shown. Reason: Frameshift at positions 691, 947 and 1260. The sequence BAC37371.1 differs from that shown. Reason: Erroneous termination at position 433. Translated as Tyr. The sequence CAO78007.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAO78129.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Transducer
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide metabolic process Ref.4 Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.4 Inferred from direct assay. Source: MGI
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A2AP18-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A2AP18-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1068-1238: DTRLFPLQRP...SSSSSMSSND → GKAPGGEATE...HSPMFSTVRD
     1239-1501: Missing.

Isoform 3 (identifier: A2AP18-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1068-1252: DTRLFPLQRP...LSLPSLGLCR → GKAPGGEATE...ASHALYTWHA
     1253-1501: Missing.

Isoform 4 (identifier: A2AP18-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.
     106-116: VSPRWQLSLVV → MDAGAAPQKHM

Isoform 5 (identifier: A2AP18-5)

The sequence of this isoform differs from the canonical sequence as follows:
     40-115: Missing.
     580-675: ASTNRKRVEN...KKGSKIKKVA → VSGSPGSACR...GKWWFTNVKK
     676-1501: Missing.

Isoform 6 (identifier: A2AP18-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1067-1067: R → RGKAPGGEAT...STVRDDLRVP

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1501	1501	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase eta-2		PRO_0000308959
Regions
	Domain	121 – 229	109	PH
	Domain	243 – 278	36	EF-hand 1
	Domain	279 – 315	37	EF-hand 2
	Domain	400 – 545	146	PI-PLC X-box
	Domain	707 – 821	115	PI-PLC Y-box
	Domain	826 – 933	108	C2
	Calcium binding	256 – 267	12	Potential
	Region	75 – 229	155	Necessary for plasma membrane localization
	Compositional bias	1227 – 1236	10	Poly-Ser
Sites
	Active site	415	1	By similarity
	Active site	459	1	By similarity
	Metal binding	416	1	Calcium 1; catalytic By similarity
	Metal binding	445	1	Calcium 1; catalytic By similarity
	Metal binding	447	1	Calcium 1; catalytic By similarity
	Metal binding	494	1	Calcium 1; catalytic By similarity
	Metal binding	865	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	867	1	Calcium 2 By similarity
	Metal binding	891	1	Calcium 2 By similarity
	Metal binding	920	1	Calcium 3 By similarity
	Metal binding	921	1	Calcium 3; via carbonyl oxygen By similarity
	Metal binding	922	1	Calcium 3 By similarity
	Binding site	543	1	Substrate By similarity
	Binding site	545	1	Substrate By similarity
	Binding site	734	1	Substrate By similarity
	Binding site	761	1	Substrate By similarity
Amino acid modifications
	Modified residue	676	1	Phosphoserine Ref.6
	Modified residue	686	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 105	105	Missing in isoform 4.		VSP_029075
	Alternative sequence	40 – 115	76	Missing in isoform 5.		VSP_029076
	Alternative sequence	106 – 116	11	VSPRWQLSLVV → MDAGAAPQKHM in isoform 4.		VSP_029077
	Alternative sequence	580 – 675	96	ASTNR…IKKVA → VSGSPGSACREPGVGPQHPR PILSTKGIYLPQRERGQGVR DKDRRWRVREKGKGTREGAR GICPGGRGDKGLPLDREETD RQTWPIGKWWFTNVKK in isoform 5.		VSP_029078
	Alternative sequence	676 – 1501	826	Missing in isoform 5.		VSP_029079
	Alternative sequence	1067	1	R → RGKAPGGEATEERTLAQVRS PNAPEGPGPAGMAATCMKCV VGSCAGMDVEGLRREQQPSP GPAGSHMAISHQPRARVDSL GGPCCSPSPRATPGRSKEAP KGPRARRQGPGGGSVSSDSS SPDSPGSPKVAPCQPEGAHR QQGALQGEMNALFVQKLEEI RSHSPMFSTVRDDLRVP in isoform 6.		VSP_029080
	Alternative sequence	1068 – 1252	185	DTRLF…LGLCR → GKAPGGEATEERTLAQVRSP NAPEGPGPAGMAATCMKCVV GSCAGMDVEGLQREQQPSPG PAGSHMAISHQPRARVDSLG GPCCSPSPRATPGRSKEAPK GPRARRQGPGGGSVSSDSSS PDSPGSPKVAPCQPEGAHRQ QGALQGEMNALFVQKLEEIR SHSPMFSTGKACRSAASHAL YTWHA in isoform 3.		VSP_029081
	Alternative sequence	1068 – 1238	171	DTRLF…MSSND → GKAPGGEATEERTLAQVRSP NAPEGPGPAGMAATCMKCVV GSCAGMDVEGLRREQQPSPG PAGSHMAISHQPRARVDSLG GPCCSPSPRATPGRSKEAPK GPRARRQGPGGGSVSSDSSS PDSPGSPKVAPCQPEGAHRQ QGALQGEMNALFVQKLEEIR SHSPMFSTVRD in isoform 2.		VSP_029082
	Alternative sequence	1239 – 1501	263	Missing in isoform 2.		VSP_029083
	Alternative sequence	1253 – 1501	249	Missing in isoform 3.		VSP_029084
Experimental info
	Mutagenesis	415	1	H → A: Inhibition of activity. Ref.4
	Sequence conflict	340	1	F → L in BAC34011. Ref.3
	Sequence conflict	664 – 665	2	RK → HR in AAH40465. Ref.5
	Sequence conflict	781	1	N → D in BAC32005. Ref.3
	Sequence conflict	1258	1	P → A in BAC32005. Ref.3
	Sequence conflict	1495	1	G → S in BAC32005. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 21, 2007. Version 2. Checksum: 6C2CA0E2864B327B	FASTA	1,501	164,297
	10 20 30 40 50 60 MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRG RGEKGIPEEP LCQLTPQLGL 70 80 90 100 110 120 SLRVPFGLGD YGLDMPGPQP SAASQTTGAV ACLAEVLLWV GGSVVVSPRW QLSLVVERCM 130 140 150 160 170 180 SAMQEGTQMV KLRGSSKGLV RFYYLDEHRS CLRWRPSRKN EKAKISIDSI QEVSEGRQSE 190 200 210 220 230 240 IFQRYPDSSF DPNCCFSIYH GSHRESLDLV SPSSEEARTW VTGLRYLMAG ISDEDSLARR 250 260 270 280 290 300 QRTRDQWLKQ TFDEADKNGD GSLSISEVLQ LLHKLNVNLP RQRVKQMFRE ADTDDHQGTL 310 320 330 340 350 360 GFEEFCAFYK MMSTRRDLYL LMLTYSNHKD HLDASDLQRF LEVEQKMNGV TLESCQNIIE 370 380 390 400 410 420 QFEPCLENKS KGMLGIDGFT NYTRSPAGDI FNPEHNRVHQ DMTQPLSHYF ITSSHNTYLV 430 440 450 460 470 480 GDQLMSQSRV DMYAWVLQAG CRCVEVDCWD GPDGEPIVHH GYTLTSKILF KDVIETINKY 490 500 510 520 530 540 AFIKNEYPVI LSIENHCSVV QQKKMAQYLT DILGDKLDLS SVSSEDATML PSPQMLKGKI 550 560 570 580 590 600 LVKGKKLPAN ISEDAEEGEV SDEDSADEME DDCKLLNGDA STNRKRVENI AKKKLDSLIK 610 620 630 640 650 660 ESKIRDCEDP NDFSVSTLSP SGKLGRKAEA KKGQSKVEED VEAGEDSGVS RQNSRLFMSS 670 680 690 700 710 720 FSKRKKKGSK IKKVASVEEG DETLDSPGSQ SRGTARQKKT MKLSRALSDL VKYTKSVGTH 730 740 750 760 770 780 DVEIEVVSSW QVSSFSETKA HQILQQKPTQ YLRFNQHQLS RIYPSSYRVD SSNYNPQPFW 790 800 810 820 830 840 NAGCQMVALN YQSEGRMLQL NRAKFSANGD CGYVLKPQCM CQGVFNPNSE DPLPGQLKKQ 850 860 870 880 890 900 LALRIISGQQ LPKPRDSVLG DRGEIIDPFV EVEVIGLPVD CSKEQTRVVD DNGFNPMWEE 910 920 930 940 950 960 TLVFTVHMPE IALVRFLVWD HDPIGRDFIG QRTLAFSSIM PGYRHVYLEG MEEASIFVHV 970 980 990 1000 1010 1020 AVSDISGKVK QTLGLKGLFL RGTKPGSLDS HAAGQPLPRP SVSQRLLRRT ASAPTKSQKP 1030 1040 1050 1060 1070 1080 SRKGFPELAL GTQDAGSEGA ADDVAPSSPN PALEAPTQER SGSSSPRDTR LFPLQRPISP 1090 1100 1110 1120 1130 1140 LCSLEPIAEE PALGPGLPLQ AAAPTGPSQE GSQCPVGLGA KVTSSQQTSL GAFGTLQLRI 1150 1160 1170 1180 1190 1200 GGGRENEEPP LRPHNGGISS GPREGTSGRQ TDSKSRSRVP GHLPVVRRAK SEGQVLSELS 1210 1220 1230 1240 1250 1260 PTPAVYSDAT GTDRLWQRLE PGSHRDSVSS SSSMSSNDTV IDLSLPSLGL CRSRESIPGV 1270 1280 1290 1300 1310 1320 SLGRLTSRPC LASAARPDLP PVTKSKSNPN LRVAGGLPTA PDELQPRPLA PRLTGHHPRP 1330 1340 1350 1360 1370 1380 PWHHLTLVGL RDCPVSAKSK SLGDLTADDF APSFQGSTSS LSCGLGSLGV AHQVLEPGIR 1390 1400 1410 1420 1430 1440 RDALTEQLRW LTGFQQAGDI TSPTSLGPAG DGSVGGPSFL RRSSSRSQSR VRAIASRARQ 1450 1460 1470 1480 1490 1500 AQERQQRLRG QDSRGPPEEE RGTPEGACSV GHEGCVDVPM PAKGAPEQVC GAADGQLLLR L
	Isoform 2. Checksum: F6A095EB915BF370	FASTA	1,238	136,321
	10 20 30 40 50 60 MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRG RGEKGIPEEP LCQLTPQLGL 70 80 90 100 110 120 SLRVPFGLGD YGLDMPGPQP SAASQTTGAV ACLAEVLLWV GGSVVVSPRW QLSLVVERCM 130 140 150 160 170 180 SAMQEGTQMV KLRGSSKGLV RFYYLDEHRS CLRWRPSRKN EKAKISIDSI QEVSEGRQSE 190 200 210 220 230 240 IFQRYPDSSF DPNCCFSIYH GSHRESLDLV SPSSEEARTW VTGLRYLMAG ISDEDSLARR 250 260 270 280 290 300 QRTRDQWLKQ TFDEADKNGD GSLSISEVLQ LLHKLNVNLP RQRVKQMFRE ADTDDHQGTL 310 320 330 340 350 360 GFEEFCAFYK MMSTRRDLYL LMLTYSNHKD HLDASDLQRF LEVEQKMNGV TLESCQNIIE 370 380 390 400 410 420 QFEPCLENKS KGMLGIDGFT NYTRSPAGDI FNPEHNRVHQ DMTQPLSHYF ITSSHNTYLV 430 440 450 460 470 480 GDQLMSQSRV DMYAWVLQAG CRCVEVDCWD GPDGEPIVHH GYTLTSKILF KDVIETINKY 490 500 510 520 530 540 AFIKNEYPVI LSIENHCSVV QQKKMAQYLT DILGDKLDLS SVSSEDATML PSPQMLKGKI 550 560 570 580 590 600 LVKGKKLPAN ISEDAEEGEV SDEDSADEME DDCKLLNGDA STNRKRVENI AKKKLDSLIK 610 620 630 640 650 660 ESKIRDCEDP NDFSVSTLSP SGKLGRKAEA KKGQSKVEED VEAGEDSGVS RQNSRLFMSS 670 680 690 700 710 720 FSKRKKKGSK IKKVASVEEG DETLDSPGSQ SRGTARQKKT MKLSRALSDL VKYTKSVGTH 730 740 750 760 770 780 DVEIEVVSSW QVSSFSETKA HQILQQKPTQ YLRFNQHQLS RIYPSSYRVD SSNYNPQPFW 790 800 810 820 830 840 NAGCQMVALN YQSEGRMLQL NRAKFSANGD CGYVLKPQCM CQGVFNPNSE DPLPGQLKKQ 850 860 870 880 890 900 LALRIISGQQ LPKPRDSVLG DRGEIIDPFV EVEVIGLPVD CSKEQTRVVD DNGFNPMWEE 910 920 930 940 950 960 TLVFTVHMPE IALVRFLVWD HDPIGRDFIG QRTLAFSSIM PGYRHVYLEG MEEASIFVHV 970 980 990 1000 1010 1020 AVSDISGKVK QTLGLKGLFL RGTKPGSLDS HAAGQPLPRP SVSQRLLRRT ASAPTKSQKP 1030 1040 1050 1060 1070 1080 SRKGFPELAL GTQDAGSEGA ADDVAPSSPN PALEAPTQER SGSSSPRGKA PGGEATEERT 1090 1100 1110 1120 1130 1140 LAQVRSPNAP EGPGPAGMAA TCMKCVVGSC AGMDVEGLRR EQQPSPGPAG SHMAISHQPR 1150 1160 1170 1180 1190 1200 ARVDSLGGPC CSPSPRATPG RSKEAPKGPR ARRQGPGGGS VSSDSSSPDS PGSPKVAPCQ 1210 1220 1230 PEGAHRQQGA LQGEMNALFV QKLEEIRSHS PMFSTVRD
	Isoform 3. Checksum: D591E6F8D70C95D3	FASTA	1,252	137,734
	10 20 30 40 50 60 MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRG RGEKGIPEEP LCQLTPQLGL 70 80 90 100 110 120 SLRVPFGLGD YGLDMPGPQP SAASQTTGAV ACLAEVLLWV GGSVVVSPRW QLSLVVERCM 130 140 150 160 170 180 SAMQEGTQMV KLRGSSKGLV RFYYLDEHRS CLRWRPSRKN EKAKISIDSI QEVSEGRQSE 190 200 210 220 230 240 IFQRYPDSSF DPNCCFSIYH GSHRESLDLV SPSSEEARTW VTGLRYLMAG ISDEDSLARR 250 260 270 280 290 300 QRTRDQWLKQ TFDEADKNGD GSLSISEVLQ LLHKLNVNLP RQRVKQMFRE ADTDDHQGTL 310 320 330 340 350 360 GFEEFCAFYK MMSTRRDLYL LMLTYSNHKD HLDASDLQRF LEVEQKMNGV TLESCQNIIE 370 380 390 400 410 420 QFEPCLENKS KGMLGIDGFT NYTRSPAGDI FNPEHNRVHQ DMTQPLSHYF ITSSHNTYLV 430 440 450 460 470 480 GDQLMSQSRV DMYAWVLQAG CRCVEVDCWD GPDGEPIVHH GYTLTSKILF KDVIETINKY 490 500 510 520 530 540 AFIKNEYPVI LSIENHCSVV QQKKMAQYLT DILGDKLDLS SVSSEDATML PSPQMLKGKI 550 560 570 580 590 600 LVKGKKLPAN ISEDAEEGEV SDEDSADEME DDCKLLNGDA STNRKRVENI AKKKLDSLIK 610 620 630 640 650 660 ESKIRDCEDP NDFSVSTLSP SGKLGRKAEA KKGQSKVEED VEAGEDSGVS RQNSRLFMSS 670 680 690 700 710 720 FSKRKKKGSK IKKVASVEEG DETLDSPGSQ SRGTARQKKT MKLSRALSDL VKYTKSVGTH 730 740 750 760 770 780 DVEIEVVSSW QVSSFSETKA HQILQQKPTQ YLRFNQHQLS RIYPSSYRVD SSNYNPQPFW 790 800 810 820 830 840 NAGCQMVALN YQSEGRMLQL NRAKFSANGD CGYVLKPQCM CQGVFNPNSE DPLPGQLKKQ 850 860 870 880 890 900 LALRIISGQQ LPKPRDSVLG DRGEIIDPFV EVEVIGLPVD CSKEQTRVVD DNGFNPMWEE 910 920 930 940 950 960 TLVFTVHMPE IALVRFLVWD HDPIGRDFIG QRTLAFSSIM PGYRHVYLEG MEEASIFVHV 970 980 990 1000 1010 1020 AVSDISGKVK QTLGLKGLFL RGTKPGSLDS HAAGQPLPRP SVSQRLLRRT ASAPTKSQKP 1030 1040 1050 1060 1070 1080 SRKGFPELAL GTQDAGSEGA ADDVAPSSPN PALEAPTQER SGSSSPRGKA PGGEATEERT 1090 1100 1110 1120 1130 1140 LAQVRSPNAP EGPGPAGMAA TCMKCVVGSC AGMDVEGLQR EQQPSPGPAG SHMAISHQPR 1150 1160 1170 1180 1190 1200 ARVDSLGGPC CSPSPRATPG RSKEAPKGPR ARRQGPGGGS VSSDSSSPDS PGSPKVAPCQ 1210 1220 1230 1240 1250 PEGAHRQQGA LQGEMNALFV QKLEEIRSHS PMFSTGKACR SAASHALYTW HA
	Isoform 4. Checksum: E9DE7ABC3A7F10E7	FASTA	1,396	153,398
	10 20 30 40 50 60 MDAGAAPQKH MERCMSAMQE GTQMVKLRGS SKGLVRFYYL DEHRSCLRWR PSRKNEKAKI 70 80 90 100 110 120 SIDSIQEVSE GRQSEIFQRY PDSSFDPNCC FSIYHGSHRE SLDLVSPSSE EARTWVTGLR 130 140 150 160 170 180 YLMAGISDED SLARRQRTRD QWLKQTFDEA DKNGDGSLSI SEVLQLLHKL NVNLPRQRVK 190 200 210 220 230 240 QMFREADTDD HQGTLGFEEF CAFYKMMSTR RDLYLLMLTY SNHKDHLDAS DLQRFLEVEQ 250 260 270 280 290 300 KMNGVTLESC QNIIEQFEPC LENKSKGMLG IDGFTNYTRS PAGDIFNPEH NRVHQDMTQP 310 320 330 340 350 360 LSHYFITSSH NTYLVGDQLM SQSRVDMYAW VLQAGCRCVE VDCWDGPDGE PIVHHGYTLT 370 380 390 400 410 420 SKILFKDVIE TINKYAFIKN EYPVILSIEN HCSVVQQKKM AQYLTDILGD KLDLSSVSSE 430 440 450 460 470 480 DATMLPSPQM LKGKILVKGK KLPANISEDA EEGEVSDEDS ADEMEDDCKL LNGDASTNRK 490 500 510 520 530 540 RVENIAKKKL DSLIKESKIR DCEDPNDFSV STLSPSGKLG RKAEAKKGQS KVEEDVEAGE 550 560 570 580 590 600 DSGVSRQNSR LFMSSFSKRK KKGSKIKKVA SVEEGDETLD SPGSQSRGTA RQKKTMKLSR 610 620 630 640 650 660 ALSDLVKYTK SVGTHDVEIE VVSSWQVSSF SETKAHQILQ QKPTQYLRFN QHQLSRIYPS 670 680 690 700 710 720 SYRVDSSNYN PQPFWNAGCQ MVALNYQSEG RMLQLNRAKF SANGDCGYVL KPQCMCQGVF 730 740 750 760 770 780 NPNSEDPLPG QLKKQLALRI ISGQQLPKPR DSVLGDRGEI IDPFVEVEVI GLPVDCSKEQ 790 800 810 820 830 840 TRVVDDNGFN PMWEETLVFT VHMPEIALVR FLVWDHDPIG RDFIGQRTLA FSSIMPGYRH 850 860 870 880 890 900 VYLEGMEEAS IFVHVAVSDI SGKVKQTLGL KGLFLRGTKP GSLDSHAAGQ PLPRPSVSQR 910 920 930 940 950 960 LLRRTASAPT KSQKPSRKGF PELALGTQDA GSEGAADDVA PSSPNPALEA PTQERSGSSS 970 980 990 1000 1010 1020 PRDTRLFPLQ RPISPLCSLE PIAEEPALGP GLPLQAAAPT GPSQEGSQCP VGLGAKVTSS 1030 1040 1050 1060 1070 1080 QQTSLGAFGT LQLRIGGGRE NEEPPLRPHN GGISSGPREG TSGRQTDSKS RSRVPGHLPV 1090 1100 1110 1120 1130 1140 VRRAKSEGQV LSELSPTPAV YSDATGTDRL WQRLEPGSHR DSVSSSSSMS SNDTVIDLSL 1150 1160 1170 1180 1190 1200 PSLGLCRSRE SIPGVSLGRL TSRPCLASAA RPDLPPVTKS KSNPNLRVAG GLPTAPDELQ 1210 1220 1230 1240 1250 1260 PRPLAPRLTG HHPRPPWHHL TLVGLRDCPV SAKSKSLGDL TADDFAPSFQ GSTSSLSCGL 1270 1280 1290 1300 1310 1320 GSLGVAHQVL EPGIRRDALT EQLRWLTGFQ QAGDITSPTS LGPAGDGSVG GPSFLRRSSS 1330 1340 1350 1360 1370 1380 RSQSRVRAIA SRARQAQERQ QRLRGQDSRG PPEEERGTPE GACSVGHEGC VDVPMPAKGA 1390 PEQVCGAADG QLLLRL
	Isoform 5. Checksum: 4388365410ED427F	FASTA	599	67,979
	10 20 30 40 50 60 MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRV ERCMSAMQEG TQMVKLRGSS 70 80 90 100 110 120 KGLVRFYYLD EHRSCLRWRP SRKNEKAKIS IDSIQEVSEG RQSEIFQRYP DSSFDPNCCF 130 140 150 160 170 180 SIYHGSHRES LDLVSPSSEE ARTWVTGLRY LMAGISDEDS LARRQRTRDQ WLKQTFDEAD 190 200 210 220 230 240 KNGDGSLSIS EVLQLLHKLN VNLPRQRVKQ MFREADTDDH QGTLGFEEFC AFYKMMSTRR 250 260 270 280 290 300 DLYLLMLTYS NHKDHLDASD LQRFLEVEQK MNGVTLESCQ NIIEQFEPCL ENKSKGMLGI 310 320 330 340 350 360 DGFTNYTRSP AGDIFNPEHN RVHQDMTQPL SHYFITSSHN TYLVGDQLMS QSRVDMYAWV 370 380 390 400 410 420 LQAGCRCVEV DCWDGPDGEP IVHHGYTLTS KILFKDVIET INKYAFIKNE YPVILSIENH 430 440 450 460 470 480 CSVVQQKKMA QYLTDILGDK LDLSSVSSED ATMLPSPQML KGKILVKGKK LPANISEDAE 490 500 510 520 530 540 EGEVSDEDSA DEMEDDCKLL NGDVSGSPGS ACREPGVGPQ HPRPILSTKG IYLPQRERGQ 550 560 570 580 590 GVRDKDRRWR VREKGKGTRE GARGICPGGR GDKGLPLDRE ETDRQTWPIG KWWFTNVKK
	Isoform 6. Checksum: 1DECC602D0192C0D	FASTA	1,677	182,360
	10 20 30 40 50 60 MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRG RGEKGIPEEP LCQLTPQLGL 70 80 90 100 110 120 SLRVPFGLGD YGLDMPGPQP SAASQTTGAV ACLAEVLLWV GGSVVVSPRW QLSLVVERCM 130 140 150 160 170 180 SAMQEGTQMV KLRGSSKGLV RFYYLDEHRS CLRWRPSRKN EKAKISIDSI QEVSEGRQSE 190 200 210 220 230 240 IFQRYPDSSF DPNCCFSIYH GSHRESLDLV SPSSEEARTW VTGLRYLMAG ISDEDSLARR 250 260 270 280 290 300 QRTRDQWLKQ TFDEADKNGD GSLSISEVLQ LLHKLNVNLP RQRVKQMFRE ADTDDHQGTL 310 320 330 340 350 360 GFEEFCAFYK MMSTRRDLYL LMLTYSNHKD HLDASDLQRF LEVEQKMNGV TLESCQNIIE 370 380 390 400 410 420 QFEPCLENKS KGMLGIDGFT NYTRSPAGDI FNPEHNRVHQ DMTQPLSHYF ITSSHNTYLV 430 440 450 460 470 480 GDQLMSQSRV DMYAWVLQAG CRCVEVDCWD GPDGEPIVHH GYTLTSKILF KDVIETINKY 490 500 510 520 530 540 AFIKNEYPVI LSIENHCSVV QQKKMAQYLT DILGDKLDLS SVSSEDATML PSPQMLKGKI 550 560 570 580 590 600 LVKGKKLPAN ISEDAEEGEV SDEDSADEME DDCKLLNGDA STNRKRVENI AKKKLDSLIK 610 620 630 640 650 660 ESKIRDCEDP NDFSVSTLSP SGKLGRKAEA KKGQSKVEED VEAGEDSGVS RQNSRLFMSS 670 680 690 700 710 720 FSKRKKKGSK IKKVASVEEG DETLDSPGSQ SRGTARQKKT MKLSRALSDL VKYTKSVGTH 730 740 750 760 770 780 DVEIEVVSSW QVSSFSETKA HQILQQKPTQ YLRFNQHQLS RIYPSSYRVD SSNYNPQPFW 790 800 810 820 830 840 NAGCQMVALN YQSEGRMLQL NRAKFSANGD CGYVLKPQCM CQGVFNPNSE DPLPGQLKKQ 850 860 870 880 890 900 LALRIISGQQ LPKPRDSVLG DRGEIIDPFV EVEVIGLPVD CSKEQTRVVD DNGFNPMWEE 910 920 930 940 950 960 TLVFTVHMPE IALVRFLVWD HDPIGRDFIG QRTLAFSSIM PGYRHVYLEG MEEASIFVHV 970 980 990 1000 1010 1020 AVSDISGKVK QTLGLKGLFL RGTKPGSLDS HAAGQPLPRP SVSQRLLRRT ASAPTKSQKP 1030 1040 1050 1060 1070 1080 SRKGFPELAL GTQDAGSEGA ADDVAPSSPN PALEAPTQER SGSSSPRGKA PGGEATEERT 1090 1100 1110 1120 1130 1140 LAQVRSPNAP EGPGPAGMAA TCMKCVVGSC AGMDVEGLRR EQQPSPGPAG SHMAISHQPR 1150 1160 1170 1180 1190 1200 ARVDSLGGPC CSPSPRATPG RSKEAPKGPR ARRQGPGGGS VSSDSSSPDS PGSPKVAPCQ 1210 1220 1230 1240 1250 1260 PEGAHRQQGA LQGEMNALFV QKLEEIRSHS PMFSTVRDDL RVPDTRLFPL QRPISPLCSL 1270 1280 1290 1300 1310 1320 EPIAEEPALG PGLPLQAAAP TGPSQEGSQC PVGLGAKVTS SQQTSLGAFG TLQLRIGGGR 1330 1340 1350 1360 1370 1380 ENEEPPLRPH NGGISSGPRE GTSGRQTDSK SRSRVPGHLP VVRRAKSEGQ VLSELSPTPA 1390 1400 1410 1420 1430 1440 VYSDATGTDR LWQRLEPGSH RDSVSSSSSM SSNDTVIDLS LPSLGLCRSR ESIPGVSLGR 1450 1460 1470 1480 1490 1500 LTSRPCLASA ARPDLPPVTK SKSNPNLRVA GGLPTAPDEL QPRPLAPRLT GHHPRPPWHH 1510 1520 1530 1540 1550 1560 LTLVGLRDCP VSAKSKSLGD LTADDFAPSF QGSTSSLSCG LGSLGVAHQV LEPGIRRDAL 1570 1580 1590 1600 1610 1620 TEQLRWLTGF QQAGDITSPT SLGPAGDGSV GGPSFLRRSS SRSQSRVRAI ASRARQAQER 1630 1640 1650 1660 1670 QQRLRGQDSR GPPEEERGTP EGACSVGHEG CVDVPMPAKG APEQVCGAAD GQLLLRL

References

[1]	"Molecular cloning and characterization of PLC-eta2." Zhou Y., Wing M.R., Sondek J., Harden T.K. Biochem. J. 391:667-676(2005) [PubMed: 16107206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Strain: C57BL/6J.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-664 (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1501 (ISOFORM 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1501 (ISOFORM 6). Strain: C57BL/6J. Tissue: Eye, Hippocampus and Retina.
[4]	"A novel phospholipase C, PLC(eta)2, is a neuron-specific isozyme." Nakahara M., Shimozawa M., Nakamura Y., Irino Y., Morita M., Kudo Y., Fukami K. J. Biol. Chem. 280:29128-29134(2005) [PubMed: 15899900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-1501 (ISOFORM 2), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, REGION, FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF HIS-415. Strain: C57BL/6J x 129. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 624-1501 (ISOFORM 3). Tissue: Eye.
[6]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-676 AND SER-686, MASS SPECTROMETRY. Tissue: Brain.
[7]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ176851 mRNA. Translation: ABA12210.1. AL831788, BX004788 Genomic DNA. Translation: CAM16344.2. BX004788, AL831788 Genomic DNA. Translation: CAM28037.2. AL831788, BX004788 Genomic DNA. Translation: CAO78007.1. Sequence problems. BX004788, AL831788 Genomic DNA. Translation: CAO78129.1. Sequence problems. AK044619 mRNA. Translation: BAC32005.1. Frameshift. AK049970 mRNA. Translation: BAC34011.1. AK078731 mRNA. Translation: BAC37371.1. Sequence problems. AY966876 mRNA. Translation: AAY33831.1. BC040465 mRNA. Translation: AAH40465.1. BC052329 mRNA. Translation: AAH52329.1.
IPI	IPI00226764. IPI00649874. IPI00830504. IPI00875018. IPI00875482. IPI00876566.
RefSeq	NP_001106831.1. NP_780765.2.
UniGene	Mm.379458
3D structure databases
SMR	A2AP18. Positions 120-964.
ModBase	Search...
PTM databases
PhosphoSite	A2AP18.
Proteomic databases
PRIDE	A2AP18.
Genome annotation databases
Ensembl	ENSMUST00000030929; ENSMUSP00000030929; ENSMUSG00000029055; Mus musculus. [Genome view] ENSMUST00000105630; ENSMUSP00000101255; ENSMUSG00000029055; Mus musculus. [Genome view] ENSMUST00000105631; ENSMUSP00000101256; ENSMUSG00000029055; Mus musculus. [Genome view]
GeneID	269615.
KEGG	mmu:269615.
Organism-specific databases
CTD	269615.
MGI	MGI:2443078. Plch2.
Phylogenomic databases
eggNOG	maNOG16228.
HOVERGEN	A2AP18.
InParanoid	A2AP18.
OrthoDB	EOG9JWZ0J.
PhylomeDB	A2AP18.
Enzyme and pathway databases
BRENDA	3.1.4.11. 244.
Gene expression databases
Bgee	A2AP18.
CleanEx	MM_PLCH2.
Genevestigator	A2AP18.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR011993. PH_type. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR001849. Pleckstrin_homology. IPR000909. PLipase_C_PInositol-sp_X_dom. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 2 hits.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00054. EFh. 2 hits. SM00233. PH. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS50003. PH_DOMAIN. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	392931.
SOURCE	Search...

Entry information

Entry name

PLCH2_MOUSE

Accession

Primary (citable) accession number: A2AP18
Secondary accession number(s): A6PWW5 , Q3LUA7, Q4QSC7, Q80WP6, Q8BJV1, Q8BWU4, Q8BXN5, Q8CFR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	August 21, 2007
Last modified:	February 9, 2010
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)