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Protein

Filensin

Gene

Bfsp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of eye lens Source: MGI

GO - Biological processi

  • cell maturation Source: MGI
  • lens fiber cell development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Eye lens protein

Names & Taxonomyi

Protein namesi
Recommended name:
Filensin2 Publications
Alternative name(s):
Beaded filament structural protein 1
Lens fiber cell beaded-filament structural protein CP 95
Short name:
CP95
Gene namesi
Name:Bfsp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:101770. Bfsp1.

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cytoplasmcytoskeleton By similarity
  • Cytoplasmcell cortex By similarity

  • Note: Detected adjacent to the cell membrane.By similarity

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • intermediate filament Source: MGI
  • mitochondrion Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 669669FilensinPRO_0000285854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51Phosphoserine; by PKASequence analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA2AMT1.
PaxDbiA2AMT1.
PRIDEiA2AMT1.

PTM databases

iPTMnetiA2AMT1.
PhosphoSiteiA2AMT1.

Expressioni

Tissue specificityi

Detected in eye lens fiber cells (at protein level) (PubMed:21745462).1 Publication

Gene expression databases

BgeeiA2AMT1.
CleanExiMM_BFSP1.
GenevisibleiA2AMT1. MM.

Interactioni

Subunit structurei

Associates with BFSP2 to form long fibrils (By similarity). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (PubMed:21745462).By similarity1 Publication

Protein-protein interaction databases

BioGridi198340. 1 interaction.
STRINGi10090.ENSMUSP00000028907.

Structurei

3D structure databases

ProteinModelPortaliA2AMT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3333HeadAdd
BLAST
Regioni34 – 318285RodAdd
BLAST
Regioni34 – 6835Coil 1AAdd
BLAST
Regioni69 – 779Linker 1
Regioni78 – 177100Coil 1BAdd
BLAST
Regioni178 – 19417Linker 12Add
BLAST
Regioni195 – 318124Coil 2Add
BLAST
Regioni319 – 669351TailAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IHW9. Eukaryota.
ENOG410ZJHK. LUCA.
GeneTreeiENSGT00390000016976.
HOGENOMiHOG000095201.
HOVERGENiHBG050684.
InParanoidiA2AMT1.
KOiK10378.
OMAiDLYTKGR.
OrthoDBiEOG79SDWR.
PhylomeDBiA2AMT1.
TreeFamiTF331671.

Family and domain databases

InterProiIPR001664. IF.
[Graphical view]
PfamiPF00038. Filament. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2AMT1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYRRSYVFQA RQERYERAQP AGPAAQPGGT APGLAALQAL GERVAVQVQR
60 70 80 90 100
ARALQQRHAG LRRQLDAFQR LGEQPGPEDA LARHVEANLQ RARDLTAEHA
110 120 130 140 150
RLERQEAEAQ RALDEFRSKY ENECECQLVL KEMLERLNKE ADEALLRNLH
160 170 180 190 200
LQLEAQFLQA DISVAKDRYK KNLLEIQTYI TVLQQIVQTA PQVSLVTGMR
210 220 230 240 250
ESGLLMQEKL FTEREVAALQ NQLEEGREAV THLQAQKAEL QAQTTALEQA
260 270 280 290 300
IKHAHECYDE ELQLYNEQIE NLRKEIEEAE RSLERSSYDC RQLAVAQQTL
310 320 330 340 350
RNELDRYHRI IEIEGSRLSS VFIETPISLI TPSHGAPLSL GSSVKDLARA
360 370 380 390 400
VQDITAAKPR QKALPKSLPK RKEIIAQDKV EETLEDAPLK PPQEPKALQV
410 420 430 440 450
ERKAEGGSQP GAGGGHGVSP TQEGGPEDVP DGGQISKAFG KLCKVVKERV
460 470 480 490 500
SGHKEPEPEP PTDLFTKGRH VLVTGESSFV DPEFYSSSIP ARGGVVISIE
510 520 530 540 550
EDSMHHDGHV EPSPGQPMPP VENGQGVPQG REGDHSNHQQ GTDKNGLRAK
560 570 580 590 600
EPKDLEEKDD DGKKEAEGSR RPCPVIIPGP DEPSTSHSQT SGSNQGGPVG
610 620 630 640 650
PASKSSSLLA KGPSKALSIK KVEVVESIEK ISTESIQTYE ETSVIVETLI
660
GKSKGNKKLG EKSLPDTRA
Length:669
Mass (Da):73,669
Last modified:February 20, 2007 - v1
Checksum:i32DFEEC7F1098996
GO
Isoform 2 (identifier: A2AMT1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     202-207: Missing.

Show »
Length:663
Mass (Da):73,039
Checksum:iBF2CD28E57E8BB88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851Q → H in BAA24139 (PubMed:9409766).Curated
Sequence conflicti291 – 2911R → S in BAA24139 (PubMed:9409766).Curated
Sequence conflicti337 – 3371P → S in BAA24139 (PubMed:9409766).Curated
Sequence conflicti394 – 3952EP → TR in BAA24139 (PubMed:9409766).Curated
Sequence conflicti440 – 4401G → R in BAE25294 (PubMed:16141072).Curated
Sequence conflicti463 – 4631D → N in BAA24139 (PubMed:9409766).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei202 – 2076Missing in isoform 2. 2 PublicationsVSP_024920

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003147 mRNA. Translation: BAA24139.1.
AK143180 mRNA. Translation: BAE25294.1.
AK053766 mRNA. Translation: BAC35514.1.
AL807801 Genomic DNA. Translation: CAM18251.1.
AL807801 Genomic DNA. Translation: CAM18252.1.
BC057580 mRNA. Translation: AAH57580.1.
AB010375 Genomic DNA. Translation: BAA28377.1.
CCDSiCCDS16811.1. [A2AMT1-2]
CCDS71154.1. [A2AMT1-1]
RefSeqiNP_001277990.1. NM_001291061.1. [A2AMT1-1]
NP_033881.2. NM_009751.2. [A2AMT1-2]
UniGeneiMm.275937.

Genome annotation databases

EnsembliENSMUST00000028907; ENSMUSP00000028907; ENSMUSG00000027420. [A2AMT1-2]
ENSMUST00000099296; ENSMUSP00000096899; ENSMUSG00000027420. [A2AMT1-1]
GeneIDi12075.
KEGGimmu:12075.
UCSCiuc008mqh.1. mouse. [A2AMT1-2]
uc012cfe.1. mouse. [A2AMT1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003147 mRNA. Translation: BAA24139.1.
AK143180 mRNA. Translation: BAE25294.1.
AK053766 mRNA. Translation: BAC35514.1.
AL807801 Genomic DNA. Translation: CAM18251.1.
AL807801 Genomic DNA. Translation: CAM18252.1.
BC057580 mRNA. Translation: AAH57580.1.
AB010375 Genomic DNA. Translation: BAA28377.1.
CCDSiCCDS16811.1. [A2AMT1-2]
CCDS71154.1. [A2AMT1-1]
RefSeqiNP_001277990.1. NM_001291061.1. [A2AMT1-1]
NP_033881.2. NM_009751.2. [A2AMT1-2]
UniGeneiMm.275937.

3D structure databases

ProteinModelPortaliA2AMT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198340. 1 interaction.
STRINGi10090.ENSMUSP00000028907.

PTM databases

iPTMnetiA2AMT1.
PhosphoSiteiA2AMT1.

Proteomic databases

MaxQBiA2AMT1.
PaxDbiA2AMT1.
PRIDEiA2AMT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028907; ENSMUSP00000028907; ENSMUSG00000027420. [A2AMT1-2]
ENSMUST00000099296; ENSMUSP00000096899; ENSMUSG00000027420. [A2AMT1-1]
GeneIDi12075.
KEGGimmu:12075.
UCSCiuc008mqh.1. mouse. [A2AMT1-2]
uc012cfe.1. mouse. [A2AMT1-1]

Organism-specific databases

CTDi631.
MGIiMGI:101770. Bfsp1.

Phylogenomic databases

eggNOGiENOG410IHW9. Eukaryota.
ENOG410ZJHK. LUCA.
GeneTreeiENSGT00390000016976.
HOGENOMiHOG000095201.
HOVERGENiHBG050684.
InParanoidiA2AMT1.
KOiK10378.
OMAiDLYTKGR.
OrthoDBiEOG79SDWR.
PhylomeDBiA2AMT1.
TreeFamiTF331671.

Miscellaneous databases

PROiA2AMT1.
SOURCEiSearch...

Gene expression databases

BgeeiA2AMT1.
CleanExiMM_BFSP1.
GenevisibleiA2AMT1. MM.

Family and domain databases

InterProiIPR001664. IF.
[Graphical view]
PfamiPF00038. Filament. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure and sequence comparisons of the eye lens specific protein, filensin, from rat and mouse: implications for protein classification and assembly."
    Masaki S., Quinlan R.A.
    Gene 201:11-20(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: 129/SvJ.
    Tissue: Lens.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Eye.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "Identification and functional analysis of the mouse lens filensin gene promoter."
    Masaki S., Kamachi Y., Quinlan R.A., Yonezawa S., Kondoh H.
    Gene 214:77-86(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Strain: 129/SvJ.
  6. "Periaxin is required for hexagonal geometry and membrane organization of mature lens fibers."
    Maddala R., Skiba N.P., Lalane R. III, Sherman D.L., Brophy P.J., Rao P.V.
    Dev. Biol. 357:179-190(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, IDENTIFICATION IN A COMPLEX WITH EZR; AHNAK; PRX; BFSP2; ANK2; PLEC; VIM AND SPECTRIN.

Entry informationi

Entry nameiBFSP1_MOUSE
AccessioniPrimary (citable) accession number: A2AMT1
Secondary accession number(s): O54770
, Q3UPV0, Q6PFF8, Q8BKB1, Q9QWM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 20, 2007
Last modified: June 8, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.