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Protein

Protein AF-9

Gene

Mllt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AF-9
Alternative name(s):
Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein homolog
Gene namesi
Name:Mllt3
Synonyms:Af9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917372. Mllt3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Protein AF-9PRO_0000305124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei289 – 2891PhosphoserineBy similarity
Modified residuei295 – 2951PhosphoserineBy similarity
Cross-linki340 – 340Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei413 – 4131PhosphoserineCombined sources
Modified residuei420 – 4201PhosphoserineBy similarity
Modified residuei484 – 4841PhosphoserineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiA2AM29.
MaxQBiA2AM29.
PaxDbiA2AM29.
PRIDEiA2AM29.

PTM databases

PhosphoSiteiA2AM29.
SwissPalmiA2AM29.

Expressioni

Tissue specificityi

Ubiquitously expressed. Strong expression in the spleen.1 Publication

Gene expression databases

BgeeiA2AM29.
ExpressionAtlasiA2AM29. baseline and differential.
GenevisibleiA2AM29. MM.

Interactioni

Subunit structurei

Component of the super elongation complex (SEC), at least composed of EAF1, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ALKBH4 and BCOR (By similarity). Interacts with CBX8.By similarity1 Publication

Protein-protein interaction databases

BioGridi213873. 7 interactions.
DIPiDIP-60099N.
STRINGi10090.ENSMUSP00000077232.

Structurei

3D structure databases

ProteinModelPortaliA2AM29.
SMRiA2AM29. Positions 1-138, 461-569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 112105YEATSPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi296 – 3016Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi149 – 19446Poly-SerAdd
BLAST
Compositional biasi384 – 3929Poly-Ser
Compositional biasi467 – 4704Poly-Pro

Sequence similaritiesi

Contains 1 YEATS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3149. Eukaryota.
COG5033. LUCA.
GeneTreeiENSGT00440000039936.
HOGENOMiHOG000033834.
HOVERGENiHBG004191.
InParanoidiA2AM29.
KOiK15187.
OMAiPNRSVHT.
OrthoDBiEOG71G9V5.
PhylomeDBiA2AM29.
TreeFamiTF314586.

Family and domain databases

InterProiIPR005033. YEATS.
[Graphical view]
PANTHERiPTHR23195. PTHR23195. 1 hit.
PfamiPF03366. YEATS. 1 hit.
[Graphical view]
PROSITEiPS51037. YEATS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2AM29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSCAVQVK LELGHRAQVR KKPTVEGFTH DWMVFVRGPE HSNIQHFVEK
60 70 80 90 100
VVFHLHESFP RPKRVCKDPP YKVEESGYAG FILPIEVYFK NKEEPKKVRF
110 120 130 140 150
DYDLFLHLEG HPPVNHLRCE KLTFNNPTED FRRKLLKAGG DPNRSIHTSS
160 170 180 190 200
SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS TSFSKPHKLM
210 220 230 240 250
KEHKEKPSKD SREHKSAFKE PSRDHNKSSK DSSKKPKENK PLKEEKIVPK
260 270 280 290 300
MAFKEPKPMS KEPKADSNLL TVTSGQQDKK APSKRPPASD SEELSAKKRK
310 320 330 340 350
KSSSEALFKS FSSAPPLILT CSADKKQIKD KSHVKMGKVK IESETSEKKK
360 370 380 390 400
SMLPPFDDIV DPNDSDVEEN MSSKSDSEQP SPASSSSSSS SSFTPSQTRQ
410 420 430 440 450
QGPLRSIMKD LHSDDNEEES DEAEDNDNDS EMERPVNRGG SRSRRVSLSD
460 470 480 490 500
GSDSESSSAS SPLHHEPPPP LLKTNNNQIL EVKSPIKQSK SDKQIKNGEC
510 520 530 540 550
DKAYLDELVE LHRRLMTLRE RHILQQIVNL IEETGHFHIT NTTFDFDLCS
560
LDKTTVRKLQ SYLETSGTS
Length:569
Mass (Da):63,375
Last modified:February 20, 2007 - v1
Checksum:i120A0604B13675EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti157 – 1571S → G in AAK28536 (PubMed:11439343).Curated
Sequence conflicti164 – 1641S → G in AAK28536 (PubMed:11439343).Curated
Sequence conflicti172 – 1721S → N in AAK28536 (PubMed:11439343).Curated
Sequence conflicti176 – 1761S → G in AAK28536 (PubMed:11439343).Curated
Sequence conflicti179 – 19012Missing in AAH21420 (PubMed:15489334).CuratedAdd
BLAST
Sequence conflicti352 – 3521M → T in AAH21420 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL831756, AL806533, AL929524 Genomic DNA. Translation: CAM20202.1.
AL929524, AL806533, AL831756 Genomic DNA. Translation: CAM24441.1.
AL806533, AL831756, AL929524 Genomic DNA. Translation: CAM25293.1.
BC021420 mRNA. Translation: AAH21420.1.
AF333960 mRNA. Translation: AAK28536.1.
CCDSiCCDS38796.1.
RefSeqiNP_081602.3. NM_027326.3.
UniGeneiMm.288898.
Mm.472285.

Genome annotation databases

EnsembliENSMUST00000078090; ENSMUSP00000077232; ENSMUSG00000028496.
GeneIDi70122.
KEGGimmu:70122.
UCSCiuc008tmp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL831756, AL806533, AL929524 Genomic DNA. Translation: CAM20202.1.
AL929524, AL806533, AL831756 Genomic DNA. Translation: CAM24441.1.
AL806533, AL831756, AL929524 Genomic DNA. Translation: CAM25293.1.
BC021420 mRNA. Translation: AAH21420.1.
AF333960 mRNA. Translation: AAK28536.1.
CCDSiCCDS38796.1.
RefSeqiNP_081602.3. NM_027326.3.
UniGeneiMm.288898.
Mm.472285.

3D structure databases

ProteinModelPortaliA2AM29.
SMRiA2AM29. Positions 1-138, 461-569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213873. 7 interactions.
DIPiDIP-60099N.
STRINGi10090.ENSMUSP00000077232.

PTM databases

PhosphoSiteiA2AM29.
SwissPalmiA2AM29.

Proteomic databases

EPDiA2AM29.
MaxQBiA2AM29.
PaxDbiA2AM29.
PRIDEiA2AM29.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078090; ENSMUSP00000077232; ENSMUSG00000028496.
GeneIDi70122.
KEGGimmu:70122.
UCSCiuc008tmp.1. mouse.

Organism-specific databases

CTDi4300.
MGIiMGI:1917372. Mllt3.

Phylogenomic databases

eggNOGiKOG3149. Eukaryota.
COG5033. LUCA.
GeneTreeiENSGT00440000039936.
HOGENOMiHOG000033834.
HOVERGENiHBG004191.
InParanoidiA2AM29.
KOiK15187.
OMAiPNRSVHT.
OrthoDBiEOG71G9V5.
PhylomeDBiA2AM29.
TreeFamiTF314586.

Miscellaneous databases

ChiTaRSiMllt3. mouse.
NextBioi331032.
PROiA2AM29.
SOURCEiSearch...

Gene expression databases

BgeeiA2AM29.
ExpressionAtlasiA2AM29. baseline and differential.
GenevisibleiA2AM29. MM.

Family and domain databases

InterProiIPR005033. YEATS.
[Graphical view]
PANTHERiPTHR23195. PTHR23195. 1 hit.
PfamiPF03366. YEATS. 1 hit.
[Graphical view]
PROSITEiPS51037. YEATS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  3. "The polycomb protein MPc3 interacts with AF9, an MLL fusion partner in t(9;11)(p22;q23) acute leukemias."
    Hemenway C.S., de Erkenez A.C., Gould G.C.D.
    Oncogene 20:3798-3805(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-569, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH CBX8.
    Strain: Swiss Webster / NIH.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiAF9_MOUSE
AccessioniPrimary (citable) accession number: A2AM29
Secondary accession number(s): Q8VDR6, Q99MK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 20, 2007
Last modified: May 11, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.