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Protein

Protein Shroom2

Gene

Shroom2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actin filament binding Source: UniProtKB
  • beta-catenin binding Source: UniProtKB
  • protein domain specific binding Source: MGI

GO - Biological processi

  • apical protein localization Source: UniProtKB
  • brain development Source: UniProtKB
  • camera-type eye development Source: UniProtKB
  • camera-type eye morphogenesis Source: UniProtKB
  • cell-cell junction maintenance Source: MGI
  • cell migration Source: UniProtKB
  • cellular pigment accumulation Source: UniProtKB
  • ear development Source: UniProtKB
  • establishment of melanosome localization Source: UniProtKB
  • eye pigment granule organization Source: UniProtKB
  • lens morphogenesis in camera-type eye Source: UniProtKB
  • melanosome organization Source: UniProtKB
  • negative regulation of actin filament depolymerization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Shroom2
Alternative name(s):
Protein Apxl
Gene namesi
Name:Shroom2
Synonyms:Apxl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:107194. Shroom2.

Subcellular locationi

GO - Cellular componenti

  • apical junction complex Source: MGI
  • apical plasma membrane Source: UniProtKB
  • bicellular tight junction Source: UniProtKB
  • cell-cell adherens junction Source: UniProtKB
  • cell-cell junction Source: MGI
  • cell cortex Source: MGI
  • cytoplasm Source: MGI
  • cytoskeleton Source: UniProtKB
  • extracellular exosome Source: MGI
  • microtubule Source: UniProtKB-KW
  • neuronal cell body Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002860631 – 1481Protein Shroom2Add BLAST1481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei236PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1
Modified residuei330PhosphoserineCombined sources1
Modified residuei935PhosphoserineCombined sources1
Modified residuei959PhosphoserineCombined sources1
Modified residuei960PhosphoserineCombined sources1
Modified residuei962PhosphoserineCombined sources1
Modified residuei963PhosphothreonineCombined sources1
Modified residuei1041PhosphoserineBy similarity1
Modified residuei1043PhosphoserineBy similarity1
Modified residuei1165PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA2ALU4.
PaxDbiA2ALU4.
PeptideAtlasiA2ALU4.
PRIDEiA2ALU4.

PTM databases

iPTMnetiA2ALU4.
PhosphoSitePlusiA2ALU4.

Expressioni

Tissue specificityi

Present in kidney tubules and in the vasculature of many tissues (at protein level).1 Publication

Developmental stagei

Present in the vasculature and in multiple epithelial populations at E10.5. Present in the cell junctions of the retinal pigmented epithelium at E15.5 (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000045180.
CleanExiMM_SHROOM2.
ExpressionAtlasiA2ALU4. baseline and differential.
GenevisibleiA2ALU4. MM.

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actin filament binding Source: UniProtKB
  • beta-catenin binding Source: UniProtKB
  • protein domain specific binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098701.

Structurei

3D structure databases

ProteinModelPortaliA2ALU4.
SMRiA2ALU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 108PDZPROSITE-ProRule annotationAdd BLAST83
Domaini705 – 807ASD1PROSITE-ProRule annotationAdd BLAST103
Domaini1185 – 1476ASD2PROSITE-ProRule annotationAdd BLAST292

Domaini

The ASD1 domain mediates F-actin binding.

Sequence similaritiesi

Belongs to the shroom family.Curated
Contains 1 ASD1 domain.PROSITE-ProRule annotation
Contains 1 ASD2 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410II86. Eukaryota.
ENOG41113GY. LUCA.
GeneTreeiENSGT00530000063061.
HOGENOMiHOG000008235.
HOVERGENiHBG096290.
InParanoidiA2ALU4.
KOiK18625.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR014800. ASD1_dom.
IPR014799. ASD2_dom.
IPR001478. PDZ.
IPR027685. Shroom_fam.
[Graphical view]
PANTHERiPTHR15012. PTHR15012. 1 hit.
PfamiPF08688. ASD1. 1 hit.
PF08687. ASD2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51306. ASD1. 1 hit.
PS51307. ASD2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2ALU4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGAEPRARP ERLAEAEAPA TDGVRLVEVQ LSGGAPWGFT LKGGREHGEP
60 70 80 90 100
LVITKIEEGS KAAAVDKLLA GDEIVAINDV SLSGFRQEAI CLVKGSHKTL
110 120 130 140 150
KLVVKRKSDP SWRPHSWHAT KYFDVHPEPA ASLFLNTSGS PSWKSQHQAS
160 170 180 190 200
SSSHDLSGSW EHTSLQRTSD HFSSMGSIDS LDHSSQLYPS GHLSSAKSNS
210 220 230 240 250
SIDHLGGHSK RDSAYGSFST CSSTPDHTLP KADASSTENI LYKVGLWEAS
260 270 280 290 300
RPGSSRQSQS TGDPQGLQDR PSCFIPRVPG NSSKSPRPED NVEPKIATHG
310 320 330 340 350
RSNFGPVWYV PDKKKAPSPP PLGLPLRSDS FSVAARGHEK ARGPPFSDLA
360 370 380 390 400
SMQHFITLPH VQPRGDHRME TTDRQWKLTH LSSGKEIGNV GYQSEGHLDC
410 420 430 440 450
RWLCSDDRAG RPSGPPGRLQ FSDVHFLKSY HGSQHQQQCS DESPRAPSSP
460 470 480 490 500
RELLHITPGG GLQEPPEPSQ DDNPTQVRWP GSAHQKLDDR GRSHYFPGSL
510 520 530 540 550
RQPVQGSAQV VIPRGDYWHS DTTPVDLEYP LLRPVGQRTY LQQHEETPAS
560 570 580 590 600
HEKEGYHQLN AGIEGCCSGI QEPPRASRTV RTGLQCPSND FKLVDGESGR
610 620 630 640 650
ISRQRTPMLH SLTQDGTWRP GNSKDCGNDK PPLFDAQVGK PTRRSDRFAT
660 670 680 690 700
TLRNEIQMRR AKLQKSKSTV TLAGDSEAED CAGDWRADVG AVPEGSFPST
710 720 730 740 750
YKEHLKEAQT RVLKATSFQR RDLDPTPADQ YSGPSEHRTF DHSASSSLSS
760 770 780 790 800
FPGEPDSAPR FCETGLAKAP SSGVGVPHVL RIGGRKRFTA EQKLKSYSEP
810 820 830 840 850
EKINEVGLSG DHRPHPTVRT PEDTVGTFAD RWKFFEETSK SLLQKAGHRQ
860 870 880 890 900
VHCGLPREKA ERPQTGHHEC ESTEPWFQKR SLATSCGEIL SDRKVEKASE
910 920 930 940 950
KLNPPRRLGT FAEYQASWKE QKKPLEARSS GRYHSADDIL DAGLDQQQRP
960 970 980 990 1000
QYIHERSRSS PSTDHYSQEV PVEPNRQAED SGDHKEAILC TLQAEEGCSA
1010 1020 1030 1040 1050
PSAQPQDSQH VNEDTTFPQP ETQLSSKCQH LQTSAMETSR SPSPQFAPQK
1060 1070 1080 1090 1100
LTDKPPLLIH EDNSARIERV MDNNTTVKMV PIKIVHSESQ PEKESRQSLA
1110 1120 1130 1140 1150
CPAELPPLPS GLERDQIKTL STSEQCYSRF CVYTRQEVEA PHRARPPEPR
1160 1170 1180 1190 1200
PPSTPAPPVR DSCSSPPSLN YGKAKEKTMD DLKSEELARE IVGKDKSLAD
1210 1220 1230 1240 1250
ILDPSVKIKT TMDLMEGIFP KDEYLLEEAQ QRRKLLPKVP LPRVTEDKKQ
1260 1270 1280 1290 1300
DPGMPGVVSL ATNSTYYSTS APKAELLIKM KDLQEPEEYS AGDLDHDLSV
1310 1320 1330 1340 1350
KKQELIDSIS RKLQVLREAR ESLLEDIQAN NALGDEVEAI VKDVCKPNEF
1360 1370 1380 1390 1400
DKFRMFIGDL DKVVNLLLSL SGRLARVENA LNNLDDNPSP GDRQSLLEKQ
1410 1420 1430 1440 1450
RVLTQQHEDA KELKENLDRR ERIVFDILAT YLSEENLADY EHFVKMKSAL
1460 1470 1480
IIEQRELEDK IHLGEEQLKC LFDSLQPERS K
Length:1,481
Mass (Da):164,703
Last modified:February 20, 2007 - v1
Checksum:i3C5C96100263E393
GO
Isoform 2 (identifier: A2ALU4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1035: Missing.

Show »
Length:446
Mass (Da):50,643
Checksum:iCB52446CCA61912F
GO

Sequence cautioni

The sequence ABD19518 differs from that shown. Reason: Frameshift at positions 1238, 1244 and 1263.Curated
The sequence BAC26404 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC27781 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274F → S in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti458P → S in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti603R → C in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti1012N → K in BAC27781 (PubMed:16141072).Curated1
Sequence conflicti1100A → S in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti1227E → K in ABD19518 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0249641 – 1035Missing in isoform 2. 1 PublicationAdd BLAST1035

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ358971 mRNA. Translation: ABD19518.1. Frameshift.
AK029338 mRNA. Translation: BAC26404.1. Different initiation.
AK032256 mRNA. Translation: BAC27781.1. Different initiation.
AK049850 mRNA. Translation: BAC33957.1.
AL805960, AL807777 Genomic DNA. Translation: CAM17589.1.
AL807777, AL805960 Genomic DNA. Translation: CAM22989.1.
CCDSiCCDS72455.1. [A2ALU4-1]
RefSeqiNP_001277613.1. NM_001290684.1.
NP_001277614.1. NM_001290685.1.
NP_001277615.1. NM_001290686.1. [A2ALU4-2]
NP_001277616.1. NM_001290687.1. [A2ALU4-2]
NP_766029.2. NM_172441.3.
XP_011246074.1. XM_011247772.1. [A2ALU4-2]
XP_011246075.1. XM_011247773.1. [A2ALU4-2]
XP_011246076.1. XM_011247774.2. [A2ALU4-2]
XP_011246077.1. XM_011247775.2. [A2ALU4-2]
XP_011246078.1. XM_011247776.2. [A2ALU4-2]
XP_011246079.1. XM_011247777.2. [A2ALU4-2]
UniGeneiMm.40796.

Genome annotation databases

EnsembliENSMUST00000062317; ENSMUSP00000057500; ENSMUSG00000045180. [A2ALU4-1]
GeneIDi110380.
KEGGimmu:110380.
UCSCiuc009uqj.2. mouse. [A2ALU4-2]
uc009uqm.2. mouse. [A2ALU4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ358971 mRNA. Translation: ABD19518.1. Frameshift.
AK029338 mRNA. Translation: BAC26404.1. Different initiation.
AK032256 mRNA. Translation: BAC27781.1. Different initiation.
AK049850 mRNA. Translation: BAC33957.1.
AL805960, AL807777 Genomic DNA. Translation: CAM17589.1.
AL807777, AL805960 Genomic DNA. Translation: CAM22989.1.
CCDSiCCDS72455.1. [A2ALU4-1]
RefSeqiNP_001277613.1. NM_001290684.1.
NP_001277614.1. NM_001290685.1.
NP_001277615.1. NM_001290686.1. [A2ALU4-2]
NP_001277616.1. NM_001290687.1. [A2ALU4-2]
NP_766029.2. NM_172441.3.
XP_011246074.1. XM_011247772.1. [A2ALU4-2]
XP_011246075.1. XM_011247773.1. [A2ALU4-2]
XP_011246076.1. XM_011247774.2. [A2ALU4-2]
XP_011246077.1. XM_011247775.2. [A2ALU4-2]
XP_011246078.1. XM_011247776.2. [A2ALU4-2]
XP_011246079.1. XM_011247777.2. [A2ALU4-2]
UniGeneiMm.40796.

3D structure databases

ProteinModelPortaliA2ALU4.
SMRiA2ALU4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098701.

PTM databases

iPTMnetiA2ALU4.
PhosphoSitePlusiA2ALU4.

Proteomic databases

MaxQBiA2ALU4.
PaxDbiA2ALU4.
PeptideAtlasiA2ALU4.
PRIDEiA2ALU4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062317; ENSMUSP00000057500; ENSMUSG00000045180. [A2ALU4-1]
GeneIDi110380.
KEGGimmu:110380.
UCSCiuc009uqj.2. mouse. [A2ALU4-2]
uc009uqm.2. mouse. [A2ALU4-1]

Organism-specific databases

CTDi357.
MGIiMGI:107194. Shroom2.

Phylogenomic databases

eggNOGiENOG410II86. Eukaryota.
ENOG41113GY. LUCA.
GeneTreeiENSGT00530000063061.
HOGENOMiHOG000008235.
HOVERGENiHBG096290.
InParanoidiA2ALU4.
KOiK18625.

Miscellaneous databases

PROiA2ALU4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045180.
CleanExiMM_SHROOM2.
ExpressionAtlasiA2ALU4. baseline and differential.
GenevisibleiA2ALU4. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR014800. ASD1_dom.
IPR014799. ASD2_dom.
IPR001478. PDZ.
IPR027685. Shroom_fam.
[Graphical view]
PANTHERiPTHR15012. PTHR15012. 1 hit.
PfamiPF08688. ASD1. 1 hit.
PF08687. ASD2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51306. ASD1. 1 hit.
PS51307. ASD2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSHRM2_MOUSE
AccessioniPrimary (citable) accession number: A2ALU4
Secondary accession number(s): Q2EY23
, Q8C7N1, Q8CCR2, Q8CDZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.