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Protein

DDB1- and CUL4-associated factor 10

Gene

Dcaf10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 10
Alternative name(s):
WD repeat-containing protein 32
Gene namesi
Name:Dcaf10
Synonyms:Wdr32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2140179. Dcaf10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566DDB1- and CUL4-associated factor 10PRO_0000306834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineCombined sources
Modified residuei57 – 571PhosphoserineCombined sources
Modified residuei67 – 671PhosphoserineBy similarity
Modified residuei96 – 961PhosphoserineCombined sources
Modified residuei99 – 991PhosphoserineBy similarity
Modified residuei100 – 1001PhosphoserineCombined sources
Modified residuei356 – 3561PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2AKB9.
MaxQBiA2AKB9.
PaxDbiA2AKB9.
PeptideAtlasiA2AKB9.
PRIDEiA2AKB9.

PTM databases

iPTMnetiA2AKB9.
PhosphoSiteiA2AKB9.

Expressioni

Gene expression databases

BgeeiA2AKB9.
CleanExiMM_WDR32.
ExpressionAtlasiA2AKB9. baseline and differential.
GenevisibleiA2AKB9. MM.

Interactioni

Subunit structurei

Interacts with DDB1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117082.

Structurei

3D structure databases

ProteinModelPortaliA2AKB9.
SMRiA2AKB9. Positions 157-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati173 – 21240WD 1Add
BLAST
Repeati216 – 25439WD 2Add
BLAST
Repeati258 – 29740WD 3Add
BLAST
Repeati303 – 34240WD 4Add
BLAST
Repeati415 – 45541WD 5Add
BLAST
Repeati477 – 51539WD 6Add
BLAST
Repeati533 – 56634WD 7Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 6866Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat DCAF10 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
ENOG410XP1Z. LUCA.
GeneTreeiENSGT00390000012666.
HOVERGENiHBG055755.
InParanoidiA2AKB9.
KOiK11802.
OMAiHGNCITS.
OrthoDBiEOG769ZJH.
PhylomeDBiA2AKB9.
TreeFamiTF323434.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000772. Ricin_B_lectin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2AKB9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPFGPHSPG GDETAGAEEP PPLGGPAAAS RPPSPAPRPA SPQRGADAAS
60 70 80 90 100
PPPVAGSPRL PGGPAVSPAE RAGEFAAPGA LELSAATASA SQAKLSPSSS
110 120 130 140 150
PRRRSRPDWR AGGRSRQGLG AGLGGPGARL FGWLRERSLG RGLFVDPARD
160 170 180 190 200
NFRTMTNLYG SIHPADSVYL STRTHGAVFN LEYSPDGSVL TVACEQTEVL
210 220 230 240 250
LFDPISSKHI KTLSEAHEDC VNNIRFLDNR LFATCSDDTT IALWDLRKLN
260 270 280 290 300
TKVCTLHGHT SWVKNIEYDT NTRLLVTSGF DGNVIIWDTN RCTEDGCPHK
310 320 330 340 350
KFFHTRFLMR MRLTPDCSKM LISTSSGYLL ILHELDLTKS LEVGSYPILR
360 370 380 390 400
ARRTTSSSDL TTTSSSSGSR VSGSPCHHND SNSTEKHMSR ASQREGVSPR
410 420 430 440 450
NSLEVLTPEV PGERDRGNCI TSLQLHPKGW ATLLRCSSNT DDQEWTCVYE
460 470 480 490 500
FQEGAPVRPV SPRCSLRLTH YIEEANVGRG YIKELCFSPD GRMISSPHGY
510 520 530 540 550
GIRLLGFDKQ CSELVDCLPK EASPLRVIRS LYSHNDVVLT TKFSPTHCQI
560
ASGCLSGRVS LYQPKF
Length:566
Mass (Da):61,558
Last modified:February 20, 2007 - v1
Checksum:i4923778CDA90E6F8
GO
Isoform 2 (identifier: A2AKB9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     359-395: Missing.

Show »
Length:529
Mass (Da):57,614
Checksum:i6DFDC654E4D001EC
GO
Isoform 3 (identifier: A2AKB9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-308: Missing.

Note: No experimental confirmation available.
Show »
Length:258
Mass (Da):28,626
Checksum:i5901B43E00473C8B
GO
Isoform 4 (identifier: A2AKB9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     226-566: Missing.

Note: No experimental confirmation available.
Show »
Length:225
Mass (Da):23,289
Checksum:i028BF807BA1FE6EB
GO

Sequence cautioni

The sequence AAH27317.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH85101.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC38912.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAM20443.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021F → L in BAE38101 (PubMed:16141072).Curated
Sequence conflicti235 – 2351C → S in AAH27317 (PubMed:15489334).Curated
Sequence conflicti323 – 3231S → P in BAC38912 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 308308Missing in isoform 3. 1 PublicationVSP_028514Add
BLAST
Alternative sequencei226 – 566341Missing in isoform 4. 1 PublicationVSP_028515Add
BLAST
Alternative sequencei359 – 39537Missing in isoform 2. CuratedVSP_028516Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045241 mRNA. Translation: BAC32276.1.
AK076417 mRNA. Translation: BAC36330.1.
AK083428 mRNA. Translation: BAC38912.1. Different initiation.
AK165246 mRNA. Translation: BAE38101.1.
AL772376, AL772285 Genomic DNA. Translation: CAM20447.1.
AL772285, AL772376 Genomic DNA. Translation: CAM26867.1.
AL772376 Genomic DNA. Translation: CAM20443.1. Sequence problems.
BC027317 mRNA. Translation: AAH27317.1. Different initiation.
BC085101 mRNA. Translation: AAH85101.1. Different initiation.
CCDSiCCDS38754.1. [A2AKB9-1]
RefSeqiNP_694807.2. NM_153167.2. [A2AKB9-1]
UniGeneiMm.317668.

Genome annotation databases

EnsembliENSMUST00000155551; ENSMUSP00000117082; ENSMUSG00000035572. [A2AKB9-1]
GeneIDi242418.
KEGGimmu:242418.
UCSCiuc008ssm.1. mouse. [A2AKB9-4]
uc008ssn.1. mouse. [A2AKB9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045241 mRNA. Translation: BAC32276.1.
AK076417 mRNA. Translation: BAC36330.1.
AK083428 mRNA. Translation: BAC38912.1. Different initiation.
AK165246 mRNA. Translation: BAE38101.1.
AL772376, AL772285 Genomic DNA. Translation: CAM20447.1.
AL772285, AL772376 Genomic DNA. Translation: CAM26867.1.
AL772376 Genomic DNA. Translation: CAM20443.1. Sequence problems.
BC027317 mRNA. Translation: AAH27317.1. Different initiation.
BC085101 mRNA. Translation: AAH85101.1. Different initiation.
CCDSiCCDS38754.1. [A2AKB9-1]
RefSeqiNP_694807.2. NM_153167.2. [A2AKB9-1]
UniGeneiMm.317668.

3D structure databases

ProteinModelPortaliA2AKB9.
SMRiA2AKB9. Positions 157-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117082.

PTM databases

iPTMnetiA2AKB9.
PhosphoSiteiA2AKB9.

Proteomic databases

EPDiA2AKB9.
MaxQBiA2AKB9.
PaxDbiA2AKB9.
PeptideAtlasiA2AKB9.
PRIDEiA2AKB9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000155551; ENSMUSP00000117082; ENSMUSG00000035572. [A2AKB9-1]
GeneIDi242418.
KEGGimmu:242418.
UCSCiuc008ssm.1. mouse. [A2AKB9-4]
uc008ssn.1. mouse. [A2AKB9-1]

Organism-specific databases

CTDi79269.
MGIiMGI:2140179. Dcaf10.

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
ENOG410XP1Z. LUCA.
GeneTreeiENSGT00390000012666.
HOVERGENiHBG055755.
InParanoidiA2AKB9.
KOiK11802.
OMAiHGNCITS.
OrthoDBiEOG769ZJH.
PhylomeDBiA2AKB9.
TreeFamiTF323434.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiDcaf10. mouse.
PROiA2AKB9.
SOURCEiSearch...

Gene expression databases

BgeeiA2AKB9.
CleanExiMM_WDR32.
ExpressionAtlasiA2AKB9. baseline and differential.
GenevisibleiA2AKB9. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000772. Ricin_B_lectin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 112-566 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo, Head and Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor and Olfactory epithelium.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50; SER-57; SER-96 AND SER-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung, Spleen and Testis.

Entry informationi

Entry nameiDCA10_MOUSE
AccessioniPrimary (citable) accession number: A2AKB9
Secondary accession number(s): A2AKV8
, Q3TNI7, Q5U4G6, Q8BHQ2, Q8BHS1, Q8BHX4, Q8K3A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 20, 2007
Last modified: July 6, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.