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Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

Man1b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2 (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei289Proton donorBy similarity1
Active sitei422By similarity1
Active sitei529Proton donorBy similarity1
Active sitei558By similarity1
Metal bindingi647CalciumBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-901032. ER Quality Control Compartment (ERQC).
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113By similarity)
Alternative name(s):
ER alpha-1,2-mannosidase
ER mannosidase 1
Short name:
ERMan1
Man9GlcNAc2-specific-processing alpha-mannosidase
Mannosidase alpha class 1B member 1
Gene namesi
Name:Man1b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2684954. Man1b1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 50CytoplasmicSequence analysisAdd BLAST50
Transmembranei51 – 71Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini72 – 658LumenalSequence analysisAdd BLAST587

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003966221 – 658Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidaseAdd BLAST658

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphoserineCombined sources1
Disulfide bondi486 ↔ 515By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiA2AJ15.
MaxQBiA2AJ15.
PaxDbiA2AJ15.
PeptideAtlasiA2AJ15.
PRIDEiA2AJ15.

PTM databases

iPTMnetiA2AJ15.
PhosphoSitePlusiA2AJ15.
SwissPalmiA2AJ15.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036646.
ExpressionAtlasiA2AJ15. baseline and differential.
GenevisibleiA2AJ15. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036996.

Structurei

3D structure databases

ProteinModelPortaliA2AJ15.
SMRiA2AJ15.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431. Eukaryota.
ENOG410XP04. LUCA.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181987.
HOVERGENiHBG052389.
InParanoidiA2AJ15.
KOiK01230.
OMAiACEGRRS.
OrthoDBiEOG091G0342.
PhylomeDBiA2AJ15.
TreeFamiTF354274.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

A2AJ15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPPPAPPPA PHRDFISVTL SLGESYDNSK SRRRRSCWRK WKQLSRLQRN
60 70 80 90 100
VILFVLGFLI LCGFLYSLHT ADQWKALSGR PAEVEKMKQE VLPVLPAPQK
110 120 130 140 150
ESAEQEGFAD ILSQKRQRHF RRGPPHLQIR PPNTVSKDGM QDDAKEREAA
160 170 180 190 200
LGKAQQEENT QRTVISWRGA VIEPEQATEL PYKRAEASIK PLVLASKIWK
210 220 230 240 250
EPAPPNERQK GVIEAFLHAW KGYQKFAWGH DELKPVSKTF SEWFGLGLTL
260 270 280 290 300
IDALDTMWIL GLKQEFKQAR KWVSENLDFQ KNVDVNLFES TIRILGGLLS
310 320 330 340 350
TYHLSGDSLF LTKAEDFGKR LMPAFTTPSK IPYSDVNIGT GFAHSPQWTS
360 370 380 390 400
DSTVAEVTSI QLEFRELSRL TGIKKFQEAV EEVTKHIHSL SGKKDGLVPM
410 420 430 440 450
FINTNSGLFT HPGVFTLGAR ADSYYEYLLK QWIQGGKKET QLLEDYVKAI
460 470 480 490 500
EGIKAHLLRQ SQPRKLTFVG ELAHGRFSAK MDHLVCFLPG TLALGVHHGL
510 520 530 540 550
PADHMDLARA LMETCYQMNQ QMETGLSPEI AHFNMYPRAD HKDVEVKPAD
560 570 580 590 600
RHNLLRPETV ESLFYLYRVT RDRKYQDWGW EILQSFNKYT RVPSGGYSSI
610 620 630 640 650
NNVQNSHKPE PRDKMESFFV GETLKYLYLL FSDDLELLSL DSCVFNTEAH

PLPIWAPA
Length:658
Mass (Da):75,150
Last modified:February 20, 2007 - v1
Checksum:i75BA990FA9B1470B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732557 Genomic DNA. Translation: CAM25606.1.
CH466542 Genomic DNA. Translation: EDL08233.1.
BC138550 mRNA. Translation: AAI38551.1.
BC138551 mRNA. Translation: AAI38552.1.
CCDSiCCDS15765.1.
RefSeqiNP_001025154.1. NM_001029983.2.
UniGeneiMm.270798.

Genome annotation databases

EnsembliENSMUST00000042390; ENSMUSP00000036996; ENSMUSG00000036646.
GeneIDi227619.
KEGGimmu:227619.
UCSCiuc008irp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732557 Genomic DNA. Translation: CAM25606.1.
CH466542 Genomic DNA. Translation: EDL08233.1.
BC138550 mRNA. Translation: AAI38551.1.
BC138551 mRNA. Translation: AAI38552.1.
CCDSiCCDS15765.1.
RefSeqiNP_001025154.1. NM_001029983.2.
UniGeneiMm.270798.

3D structure databases

ProteinModelPortaliA2AJ15.
SMRiA2AJ15.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036996.

PTM databases

iPTMnetiA2AJ15.
PhosphoSitePlusiA2AJ15.
SwissPalmiA2AJ15.

Proteomic databases

EPDiA2AJ15.
MaxQBiA2AJ15.
PaxDbiA2AJ15.
PeptideAtlasiA2AJ15.
PRIDEiA2AJ15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042390; ENSMUSP00000036996; ENSMUSG00000036646.
GeneIDi227619.
KEGGimmu:227619.
UCSCiuc008irp.2. mouse.

Organism-specific databases

CTDi11253.
MGIiMGI:2684954. Man1b1.

Phylogenomic databases

eggNOGiKOG2431. Eukaryota.
ENOG410XP04. LUCA.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181987.
HOVERGENiHBG052389.
InParanoidiA2AJ15.
KOiK01230.
OMAiACEGRRS.
OrthoDBiEOG091G0342.
PhylomeDBiA2AJ15.
TreeFamiTF354274.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-901032. ER Quality Control Compartment (ERQC).

Miscellaneous databases

ChiTaRSiMan1b1. mouse.
PROiA2AJ15.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036646.
ExpressionAtlasiA2AJ15. baseline and differential.
GenevisibleiA2AJ15. MM.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMA1B1_MOUSE
AccessioniPrimary (citable) accession number: A2AJ15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.