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Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

Man1b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2 (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei289Proton donorBy similarity1
Active sitei422By similarity1
Active sitei529Proton donorBy similarity1
Active sitei558By similarity1
Metal bindingi647CalciumBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-901032 ER Quality Control Compartment (ERQC)
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113By similarity)
Alternative name(s):
ER alpha-1,2-mannosidase
ER mannosidase 1
Short name:
ERMan1
Man9GlcNAc2-specific-processing alpha-mannosidase
Mannosidase alpha class 1B member 1
Gene namesi
Name:Man1b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2684954 Man1b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 50CytoplasmicSequence analysisAdd BLAST50
Transmembranei51 – 71Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini72 – 658LumenalSequence analysisAdd BLAST587

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003966221 – 658Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidaseAdd BLAST658

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphoserineCombined sources1
Disulfide bondi486 ↔ 515By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiA2AJ15
MaxQBiA2AJ15
PaxDbiA2AJ15
PeptideAtlasiA2AJ15
PRIDEiA2AJ15

PTM databases

iPTMnetiA2AJ15
PhosphoSitePlusiA2AJ15
SwissPalmiA2AJ15

Expressioni

Gene expression databases

BgeeiENSMUSG00000036646
ExpressionAtlasiA2AJ15 baseline and differential
GenevisibleiA2AJ15 MM

Interactioni

Protein-protein interaction databases

BioGridi230645, 1 interactor
STRINGi10090.ENSMUSP00000036996

Structurei

3D structure databases

ProteinModelPortaliA2AJ15
SMRiA2AJ15
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181987
HOVERGENiHBG052389
InParanoidiA2AJ15
KOiK01230
OMAiDHLTCYL
OrthoDBiEOG091G0342
PhylomeDBiA2AJ15
TreeFamiTF354274

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

A2AJ15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPPPAPPPA PHRDFISVTL SLGESYDNSK SRRRRSCWRK WKQLSRLQRN
60 70 80 90 100
VILFVLGFLI LCGFLYSLHT ADQWKALSGR PAEVEKMKQE VLPVLPAPQK
110 120 130 140 150
ESAEQEGFAD ILSQKRQRHF RRGPPHLQIR PPNTVSKDGM QDDAKEREAA
160 170 180 190 200
LGKAQQEENT QRTVISWRGA VIEPEQATEL PYKRAEASIK PLVLASKIWK
210 220 230 240 250
EPAPPNERQK GVIEAFLHAW KGYQKFAWGH DELKPVSKTF SEWFGLGLTL
260 270 280 290 300
IDALDTMWIL GLKQEFKQAR KWVSENLDFQ KNVDVNLFES TIRILGGLLS
310 320 330 340 350
TYHLSGDSLF LTKAEDFGKR LMPAFTTPSK IPYSDVNIGT GFAHSPQWTS
360 370 380 390 400
DSTVAEVTSI QLEFRELSRL TGIKKFQEAV EEVTKHIHSL SGKKDGLVPM
410 420 430 440 450
FINTNSGLFT HPGVFTLGAR ADSYYEYLLK QWIQGGKKET QLLEDYVKAI
460 470 480 490 500
EGIKAHLLRQ SQPRKLTFVG ELAHGRFSAK MDHLVCFLPG TLALGVHHGL
510 520 530 540 550
PADHMDLARA LMETCYQMNQ QMETGLSPEI AHFNMYPRAD HKDVEVKPAD
560 570 580 590 600
RHNLLRPETV ESLFYLYRVT RDRKYQDWGW EILQSFNKYT RVPSGGYSSI
610 620 630 640 650
NNVQNSHKPE PRDKMESFFV GETLKYLYLL FSDDLELLSL DSCVFNTEAH

PLPIWAPA
Length:658
Mass (Da):75,150
Last modified:February 20, 2007 - v1
Checksum:i75BA990FA9B1470B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732557 Genomic DNA Translation: CAM25606.1
CH466542 Genomic DNA Translation: EDL08233.1
BC138550 mRNA Translation: AAI38551.1
BC138551 mRNA Translation: AAI38552.1
CCDSiCCDS15765.1
RefSeqiNP_001025154.1, NM_001029983.2
UniGeneiMm.270798

Genome annotation databases

EnsembliENSMUST00000042390; ENSMUSP00000036996; ENSMUSG00000036646
GeneIDi227619
KEGGimmu:227619
UCSCiuc008irp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMA1B1_MOUSE
AccessioniPrimary (citable) accession number: A2AJ15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: February 20, 2007
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health