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A2AJ15

- MA1B1_MOUSE

UniProt

A2AJ15 - MA1B1_MOUSE

Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

Man1b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2 By similarity.By similarity

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Calcium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei289 – 2891Proton donorBy similarity
    Active sitei422 – 4221By similarity
    Active sitei558 – 5581By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: MGI

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: MGI
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_198606. ER Quality Control Compartment (ERQC).
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113)
    Alternative name(s):
    ER alpha-1,2-mannosidase
    ER mannosidase 1
    Short name:
    ERMan1
    Man9GlcNAc2-specific-processing alpha-mannosidase
    Mannosidase alpha class 1B member 1
    Gene namesi
    Name:Man1b1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2684954. Man1b1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 658658Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidasePRO_0000396622Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi486 ↔ 515By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiA2AJ15.
    PaxDbiA2AJ15.
    PRIDEiA2AJ15.

    PTM databases

    PhosphoSiteiA2AJ15.

    Expressioni

    Gene expression databases

    ArrayExpressiA2AJ15.
    BgeeiA2AJ15.
    GenevestigatoriA2AJ15.

    Structurei

    3D structure databases

    ProteinModelPortaliA2AJ15.
    SMRiA2AJ15. Positions 206-655.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5050CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini72 – 658587LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei51 – 7121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300315.
    GeneTreeiENSGT00390000016529.
    HOGENOMiHOG000181987.
    HOVERGENiHBG052389.
    InParanoidiA2AJ15.
    KOiK01230.
    OMAiVHFNLHA.
    OrthoDBiEOG7ZGX2S.
    PhylomeDBiA2AJ15.
    TreeFamiTF354274.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A2AJ15-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYPPPAPPPA PHRDFISVTL SLGESYDNSK SRRRRSCWRK WKQLSRLQRN    50
    VILFVLGFLI LCGFLYSLHT ADQWKALSGR PAEVEKMKQE VLPVLPAPQK 100
    ESAEQEGFAD ILSQKRQRHF RRGPPHLQIR PPNTVSKDGM QDDAKEREAA 150
    LGKAQQEENT QRTVISWRGA VIEPEQATEL PYKRAEASIK PLVLASKIWK 200
    EPAPPNERQK GVIEAFLHAW KGYQKFAWGH DELKPVSKTF SEWFGLGLTL 250
    IDALDTMWIL GLKQEFKQAR KWVSENLDFQ KNVDVNLFES TIRILGGLLS 300
    TYHLSGDSLF LTKAEDFGKR LMPAFTTPSK IPYSDVNIGT GFAHSPQWTS 350
    DSTVAEVTSI QLEFRELSRL TGIKKFQEAV EEVTKHIHSL SGKKDGLVPM 400
    FINTNSGLFT HPGVFTLGAR ADSYYEYLLK QWIQGGKKET QLLEDYVKAI 450
    EGIKAHLLRQ SQPRKLTFVG ELAHGRFSAK MDHLVCFLPG TLALGVHHGL 500
    PADHMDLARA LMETCYQMNQ QMETGLSPEI AHFNMYPRAD HKDVEVKPAD 550
    RHNLLRPETV ESLFYLYRVT RDRKYQDWGW EILQSFNKYT RVPSGGYSSI 600
    NNVQNSHKPE PRDKMESFFV GETLKYLYLL FSDDLELLSL DSCVFNTEAH 650
    PLPIWAPA 658
    Length:658
    Mass (Da):75,150
    Last modified:February 20, 2007 - v1
    Checksum:i75BA990FA9B1470B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732557 Genomic DNA. Translation: CAM25606.1.
    CH466542 Genomic DNA. Translation: EDL08233.1.
    BC138550 mRNA. Translation: AAI38551.1.
    BC138551 mRNA. Translation: AAI38552.1.
    CCDSiCCDS15765.1.
    RefSeqiNP_001025154.1. NM_001029983.2.
    UniGeneiMm.270798.

    Genome annotation databases

    EnsembliENSMUST00000042390; ENSMUSP00000036996; ENSMUSG00000036646.
    GeneIDi227619.
    KEGGimmu:227619.
    UCSCiuc008irp.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732557 Genomic DNA. Translation: CAM25606.1 .
    CH466542 Genomic DNA. Translation: EDL08233.1 .
    BC138550 mRNA. Translation: AAI38551.1 .
    BC138551 mRNA. Translation: AAI38552.1 .
    CCDSi CCDS15765.1.
    RefSeqi NP_001025154.1. NM_001029983.2.
    UniGenei Mm.270798.

    3D structure databases

    ProteinModelPortali A2AJ15.
    SMRi A2AJ15. Positions 206-655.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei A2AJ15.

    Proteomic databases

    MaxQBi A2AJ15.
    PaxDbi A2AJ15.
    PRIDEi A2AJ15.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042390 ; ENSMUSP00000036996 ; ENSMUSG00000036646 .
    GeneIDi 227619.
    KEGGi mmu:227619.
    UCSCi uc008irp.2. mouse.

    Organism-specific databases

    CTDi 11253.
    MGIi MGI:2684954. Man1b1.

    Phylogenomic databases

    eggNOGi NOG300315.
    GeneTreei ENSGT00390000016529.
    HOGENOMi HOG000181987.
    HOVERGENi HBG052389.
    InParanoidi A2AJ15.
    KOi K01230.
    OMAi VHFNLHA.
    OrthoDBi EOG7ZGX2S.
    PhylomeDBi A2AJ15.
    TreeFami TF354274.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198606. ER Quality Control Compartment (ERQC).

    Miscellaneous databases

    ChiTaRSi MAN1B1. mouse.
    NextBioi 378670.
    PROi A2AJ15.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A2AJ15.
    Bgeei A2AJ15.
    Genevestigatori A2AJ15.

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiMA1B1_MOUSE
    AccessioniPrimary (citable) accession number: A2AJ15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3