UniProtKB - A2AIV8 (CARD9_MOUSE)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Caspase recruitment domain-containing protein 9
Gene
Card9
Organism
Mus musculus (Mouse)
Status
Functioni
Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM-tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways (PubMed:17514206). Probably controls various innate immune response pathways depending on the intracellular pathogen. Controls CLEC7A (dectin-1)-mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10 (PubMed:16862125). In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2)-induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10 (PubMed:20538615). In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B (PubMed:17187069). In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells (PubMed:17450144). Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (PubMed:17486093).6 Publications
GO - Molecular functioni
- CARD domain binding Source: MGI
- protein homodimerization activity Source: MGI
GO - Biological processi
- defense response to Gram-positive bacterium Source: MGI
- defense response to virus Source: MGI
- I-kappaB kinase/NF-kappaB signaling Source: Ensembl
- innate immune response Source: UniProtKB-KW
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
- positive regulation of innate immune response Source: MGI
- positive regulation of interleukin-6 production Source: MGI
- positive regulation of JNK cascade Source: MGI
- positive regulation of stress-activated MAPK cascade Source: MGI
- positive regulation of tumor necrosis factor production Source: MGI
- regulation of apoptotic process Source: InterPro
- regulation of interleukin-2 biosynthetic process Source: MGI
- regulation of interleukin-6 biosynthetic process Source: MGI
- regulation of tumor necrosis factor biosynthetic process Source: MGI
- response to drug Source: MGI
- response to exogenous dsRNA Source: MGI
- response to fungus Source: MGI
- response to muramyl dipeptide Source: MGI
- response to peptidoglycan Source: MGI
Keywordsi
Biological process | Immunity, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-5607764 CLEC7A (Dectin-1) signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Caspase recruitment domain-containing protein 9 |
Gene namesi | Name:Card9 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2685628 Card9 |
Pathology & Biotechi
Disruption phenotypei
Mice were born at the normal Mendelian ratio without obvious anatomical defects but display impaired innate immunity (PubMed:16862125, PubMed:17187069). All mice die within 5 days after infection by C.albicans whereas more than half of the control mice survive for more than 12 days. Impaired zymosan-induced cytokine production. No defects in adaptive immunity (PubMed:16862125). Following infection by L.monocytogenes, mice fail to clear infection and show altered cytokine production (PubMed:17187069).4 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000428725 | 1 – 536 | Caspase recruitment domain-containing protein 9Add BLAST | 536 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 277 | PhosphoserineBy similarity | 1 | |
Modified residuei | 424 | PhosphoserineBy similarity | 1 | |
Modified residuei | 425 | PhosphoserineBy similarity | 1 | |
Modified residuei | 431 | PhosphoserineCombined sources | 1 | |
Modified residuei | 451 | PhosphoserineBy similarity | 1 | |
Modified residuei | 461 | PhosphoserineCombined sources | 1 | |
Modified residuei | 483 | PhosphoserineBy similarity | 1 | |
Modified residuei | 498 | PhosphoserineBy similarity | 1 | |
Modified residuei | 531 | Phosphothreonine; by CK2By similarity | 1 | |
Modified residuei | 533 | Phosphothreonine; by CK2By similarity | 1 |
Post-translational modificationi
Phosphorylated at Thr-531 and Thr-531 by CK2 following interaction with VHL, leading to inhibit the ability to activate NF-kappa-B.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | A2AIV8 |
PaxDbi | A2AIV8 |
PeptideAtlasi | A2AIV8 |
PRIDEi | A2AIV8 |
PTM databases
iPTMneti | A2AIV8 |
PhosphoSitePlusi | A2AIV8 |
Expressioni
Tissue specificityi
Expressed in myeloid cells. Not expressed in non-lymphoid organs.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000026928 |
Interactioni
Subunit structurei
Interacts with VHL; without leading to protein degradation (By similarity). Self-associates. Interacts (via CARD domain) with BCL10 (via CARD domain) (By similarity). Interacts with NOD2 (via NACHT domain)(PubMed:17187069, PubMed:24960071). Interacts with RIPK2 (PubMed:17187069).By similarity2 Publications
GO - Molecular functioni
- CARD domain binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
IntActi | A2AIV8 1 interactor. |
MINTi | A2AIV8 |
STRINGi | 10090.ENSMUSP00000028294 |
Structurei
3D structure databases
ProteinModelPortali | A2AIV8 |
SMRi | A2AIV8 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 98 | CARDPROSITE-ProRule annotationAdd BLAST | 93 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 117 – 272 | Sequence analysisAdd BLAST | 156 | |
Coiled coili | 303 – 415 | Sequence analysisAdd BLAST | 113 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG410IE3N Eukaryota ENOG410Y2EE LUCA |
GeneTreei | ENSGT00530000063108 |
HOGENOMi | HOG000231538 |
HOVERGENi | HBG058091 |
InParanoidi | A2AIV8 |
KOi | K12794 |
OMAi | PQLYKKV |
OrthoDBi | EOG091G0T58 |
PhylomeDBi | A2AIV8 |
TreeFami | TF351139 |
Family and domain databases
InterProi | View protein in InterPro IPR001315 CARD IPR011029 DEATH-like_dom_sf |
Pfami | View protein in Pfam PF00619 CARD, 1 hit |
SUPFAMi | SSF47986 SSF47986, 1 hit |
PROSITEi | View protein in PROSITE PS50209 CARD, 1 hit |
i Sequence
Sequence statusi: Complete.
A2AIV8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDYENDDEC WSTLESFRVK LISVIDPSRI TPYLRQCKVL NPDDEEQVLS
60 70 80 90 100
DPNLVIRKRK VGVLLDILQR TGHKGYVAFL ESLELYYPQL YRKVTGKEPA
110 120 130 140 150
RVFSMIIDAS GESGLTQLLM TEVMKLQKKV QDLTALLSSK DDFIKELRVK
160 170 180 190 200
DSLLRKHQER VQRLKEECEL SSAELKRCKD ENYELAMCLA HLSEEKGAAL
210 220 230 240 250
MRNRDLQLEV DRLRHSLMKA EDDCKVERKH TLKLRHAMEQ RPSQELLWEL
260 270 280 290 300
QQEKDLLQAR VQELQVSVQE GKLDRNSPYI QVLEEDWRQA LQEHQKQVST
310 320 330 340 350
IFSLRKDLRQ AETLRARCTE EKEMFELQCL ALRKDAKMYK DRIEAILLQM
360 370 380 390 400
EEVSIERDQA MASREELHAQ CTQSFQDKDK LRKLVRELGE KADELQLQLF
410 420 430 440 450
QTESRLLAAE GRLKQQQLDM LILSSDLEDS SPRNSQELSL PQDLEEDAQL
460 470 480 490 500
SDKGVLADRE SPEQPFMALN KEHLSLTHGM GPSSSEPPEK ERRRLKESFE
510 520 530
NYRRKRALRK MQNSWRQGEG DRGNTTGSDN TDTEGS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL732541 Genomic DNA No translation available. BC151023 mRNA Translation: AAI51024.1 |
CCDSi | CCDS38084.1 |
RefSeqi | NP_001032836.1, NM_001037747.1 |
UniGenei | Mm.330064 |
Genome annotation databases
Ensembli | ENSMUST00000028294; ENSMUSP00000028294; ENSMUSG00000026928 ENSMUST00000100303; ENSMUSP00000097876; ENSMUSG00000026928 |
GeneIDi | 332579 |
KEGGi | mmu:332579 |
UCSCi | uc008iut.1 mouse |
Similar proteinsi
Entry informationi
Entry namei | CARD9_MOUSE | |
Accessioni | A2AIV8Primary (citable) accession number: A2AIV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | February 20, 2007 | |
Last modified: | February 28, 2018 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |