Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bromodomain and WD repeat-containing protein 3

Gene

Brwd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (By similarity).By similarity

GO - Biological processi

  1. cytoskeleton organization Source: UniProtKB
  2. regulation of cell shape Source: UniProtKB
  3. regulation of transcription from RNA polymerase II promoter Source: GO_Central
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain and WD repeat-containing protein 3
Gene namesi
Name:Brwd3
Synonyms:Gm596
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:3029414. Brwd3.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17991799Bromodomain and WD repeat-containing protein 3PRO_0000283090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1574 – 15741PhosphoserineBy similarity
Modified residuei1576 – 15761PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA2AHJ4.
PaxDbiA2AHJ4.
PRIDEiA2AHJ4.

PTM databases

PhosphoSiteiA2AHJ4.

Expressioni

Gene expression databases

BgeeiA2AHJ4.
CleanExiMM_BRWD3.
ExpressionAtlasiA2AHJ4. baseline and differential.
GenevestigatoriA2AHJ4.

Structurei

3D structure databases

SMRiA2AHJ4. Positions 127-557, 1160-1239, 1302-1436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati86 – 12742WD 1Add
BLAST
Repeati178 – 21942WD 2Add
BLAST
Repeati221 – 25939WD 3Add
BLAST
Repeati262 – 30544WD 4Add
BLAST
Repeati316 – 35540WD 5Add
BLAST
Repeati360 – 40142WD 6Add
BLAST
Repeati421 – 46040WD 7Add
BLAST
Repeati463 – 50341WD 8Add
BLAST
Repeati511 – 55040WD 9Add
BLAST
Domaini1156 – 122671Bromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini1315 – 140995Bromo 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi764 – 7674Poly-Lys
Compositional biasi806 – 85752Ser-richAdd
BLAST
Compositional biasi903 – 9064Poly-Lys
Compositional biasi1451 – 14599Poly-Ser
Compositional biasi1599 – 162628Ser-richAdd
BLAST
Compositional biasi1666 – 171651Gly-richAdd
BLAST

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00720000108687.
HOGENOMiHOG000203884.
HOVERGENiHBG108248.
InParanoidiA2AHJ4.
KOiK11798.
OMAiXLIPRRL.
OrthoDBiEOG7N37BR.
TreeFamiTF324197.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
2.130.10.10. 3 hits.
InterProiIPR001487. Bromodomain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
SM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 2 hits.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2AHJ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPTQIEA ELYYLIARFL QSGPCNKSAQ VLVQELEEHQ LIPRRLDWEG
60 70 80 90 100
KEHRRSFEDL VAANAHIPPD YLLKICERIG PLLDKEIPQS VPGVQTLLGV
110 120 130 140 150
GRQSLLRDAK DCKSTLWNGS AFAALHRGRP PELPVNYVKP PNVVNITSAR
160 170 180 190 200
QLTGCSRFSH VFPSSAYQHI KMHKRILGHL SSVYCVAFDR SGRRIFTGSD
210 220 230 240 250
DCLVKIWATD DGRLLATLRG HSAEISDMAV NYENTLIAAG SCDKVVRVWC
260 270 280 290 300
LRTCAPVAVL QGHSASITSI QFCPSTKGTT RYLTSTGADG TICFWQWHVK
310 320 330 340 350
TMKFRDRPVK FTERSRPGVQ ISCSSFSSGG MFITTGSTDH VIRIYYLGSE
360 370 380 390 400
IPEKIAELES HTDKVVAVQF CNNGDSLRFV SGSRDGTARI WQYQQQEWKS
410 420 430 440 450
IVLDMATKMS GNNLTSAEDK VTKLKVTMVA WDRYDTTVIT AVNNFLLKVW
460 470 480 490 500
NSVTGQLLHT LSGHDDEVFV LEAHPFDQRI ILSAGHDGNI FIWDLDRGTK
510 520 530 540 550
IRNYFNMIEG QGHGAVFDCK FSPDGNHFAC TDSHGHLLLF GFGCSKYYEK
560 570 580 590 600
IPDQMFFHTD YRPLIRDANN YVLDEQTQQA PHLMPPPFLV DVDGNPHPTK
610 620 630 640 650
FQRLVPGREN CKDEQLIPQL GYVANGDGEV VEQVIGQQTN DQEESILDGI
660 670 680 690 700
IRELQREQDL RLINEGDVPH FPINRSYSVN GALSSPNMDI PSSPNIGLRR
710 720 730 740 750
SGQIEGVRQM HNNAPRSQMA TERDLMAWSR RVVVNELNSG VSRVQEECRN
760 770 780 790 800
AKGDLEVSLY TVEKKKKPSY PIQRNDYQPS CGRSLRRTQR KRQHTYLTRS
810 820 830 840 850
NIEHNSQASS QTSGVQEDSD SSSEEDETVG TSDASVEDPV VEWQSESSSS
860 870 880 890 900
DSSSEYSDWI ADAGINLQPP KRQTRQATQK IYSSSEDENL KLEDRQKKPK
910 920 930 940 950
QTKKKKGGLV SMAGEPNEEW LAPQWILDTI PRRSPFVPQM GDELIYFRQG
960 970 980 990 1000
HEAYVRAVRK SKIYSVNLQK QPWNKMDLRE QEFVKIVGIK YEIGPPTLCC
1010 1020 1030 1040 1050
LKLAFLDPIS GKMTGESFSI KYHDMPDVID FLVLHQFYNE AKERNWQIGD
1060 1070 1080 1090 1100
RFRSIIDDAW WFGTVESQQP FQPEYPDSSF QCYSVHWDNN EREKMSPWDM
1110 1120 1130 1140 1150
EPIPDGTAFP DEVGAGIPVS QEELTALLYK PQEGEWGAHS RDEECERVIQ
1160 1170 1180 1190 1200
GINNLLSLDF ASPFAVPVDL SAYPLYCTVV AYPTDLNTIK QRLENRFYRR
1210 1220 1230 1240 1250
ISALMWEVRY IEHNARTFNE PDSPIVKAAK IVTDVLLRFI GDQSCSDILD
1260 1270 1280 1290 1300
TYNKIKAEDP DSSDLEEDSE MVDLDSDGPG TSSGRRAKCR GRRQSLKCNP
1310 1320 1330 1340 1350
DAWKKQCEEL LSLIYEREDS EPFRQPADPQ SYPVQQQQEG ESSQSVPPDR
1360 1370 1380 1390 1400
QDPSLSEDYQ DGIDTPMDFS TVKETLESGN YDSPLEFYKD VRQIFSNSKA
1410 1420 1430 1440 1450
YTSNKKSRIY SMTLRLSALF ENHIKNIISD YKSAIQSQKR RRPRYRKRLR
1460 1470 1480 1490 1500
SSSSSLSSSR APSPKGKQKQ MKLQPKNDQN TSVAYARTSS PFSSPVSDAA
1510 1520 1530 1540 1550
EGVSLYLLDD EGDGPFSPSS FSGYSRSGNS HDPGKAKSFR NRVLPAKQDH
1560 1570 1580 1590 1600
SLDGPLTNVD GREPRTGAKR KLLSASEEDE SMGGEEKEMK ETKEQVHLSS
1610 1620 1630 1640 1650
SESGELGSSL SSESTSGSDS DSESTSRTDQ DYVDGDHDYS KFIQTRPKRK
1660 1670 1680 1690 1700
LRKQHTNGKR NWKTRGTGGR GRWGRWGRWS RGGRGRGGRG RGGRGRGGGG
1710 1720 1730 1740 1750
GRGRGRGRGG RGASRGSSRA KRARVADDEF DTMFSGRFSR LPRIKTRNQG
1760 1770 1780 1790
RRTVLYNDDS DNDNFVSTED PLNLGTSRSG RVRKMTEKAR VSHLMGWNY
Length:1,799
Mass (Da):202,942
Last modified:February 19, 2007 - v1
Checksum:i57E01C1ECBDD2D31
GO
Isoform 2 (identifier: A2AHJ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     272-283: FCPSTKGTTRYL → VRIMISSLPG
     284-1799: Missing.

Note: No experimental confirmation available.

Show »
Length:281
Mass (Da):31,091
Checksum:i2743DB889746285D
GO
Isoform 3 (identifier: A2AHJ4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-181: VNITSARQLT...MHKRILGHLS → GFFFVALTVL...VCHMTSYITF
     182-1799: Missing.

Note: No experimental confirmation available.

Show »
Length:181
Mass (Da):20,156
Checksum:iBB5B42040090F1B2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei144 – 18138VNITS…LGHLS → GFFFVALTVLELYVDQAGLC LSSAGIKVVCHMTSYITF in isoform 3. 1 PublicationVSP_024308Add
BLAST
Alternative sequencei182 – 17991618Missing in isoform 3. 1 PublicationVSP_024309Add
BLAST
Alternative sequencei272 – 28312FCPST…TTRYL → VRIMISSLPG in isoform 2. 1 PublicationVSP_024310Add
BLAST
Alternative sequencei284 – 17991516Missing in isoform 2. 1 PublicationVSP_024311Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050539 mRNA. Translation: BAC34314.1.
AK139563 mRNA. Translation: BAE24064.1.
AK163354 mRNA. Translation: BAE37315.1.
BX000698, AL731764 Genomic DNA. Translation: CAM25065.1.
AL731764, BX000698 Genomic DNA. Translation: CAM26827.1.
AL731764 Genomic DNA. Translation: CAM26828.1.
BC082562 mRNA. Translation: AAH82562.1.
CCDSiCCDS41100.1. [A2AHJ4-1]
RefSeqiNP_001074946.1. NM_001081477.1. [A2AHJ4-1]
UniGeneiMm.100112.
Mm.426127.

Genome annotation databases

EnsembliENSMUST00000101283; ENSMUSP00000098841; ENSMUSG00000063663. [A2AHJ4-3]
ENSMUST00000150434; ENSMUSP00000123588; ENSMUSG00000063663. [A2AHJ4-1]
GeneIDi382236.
KEGGimmu:382236.
UCSCiuc009ucm.1. mouse. [A2AHJ4-1]
uc009ucn.1. mouse. [A2AHJ4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050539 mRNA. Translation: BAC34314.1.
AK139563 mRNA. Translation: BAE24064.1.
AK163354 mRNA. Translation: BAE37315.1.
BX000698, AL731764 Genomic DNA. Translation: CAM25065.1.
AL731764, BX000698 Genomic DNA. Translation: CAM26827.1.
AL731764 Genomic DNA. Translation: CAM26828.1.
BC082562 mRNA. Translation: AAH82562.1.
CCDSiCCDS41100.1. [A2AHJ4-1]
RefSeqiNP_001074946.1. NM_001081477.1. [A2AHJ4-1]
UniGeneiMm.100112.
Mm.426127.

3D structure databases

SMRiA2AHJ4. Positions 127-557, 1160-1239, 1302-1436.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiA2AHJ4.

Proteomic databases

MaxQBiA2AHJ4.
PaxDbiA2AHJ4.
PRIDEiA2AHJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101283; ENSMUSP00000098841; ENSMUSG00000063663. [A2AHJ4-3]
ENSMUST00000150434; ENSMUSP00000123588; ENSMUSG00000063663. [A2AHJ4-1]
GeneIDi382236.
KEGGimmu:382236.
UCSCiuc009ucm.1. mouse. [A2AHJ4-1]
uc009ucn.1. mouse. [A2AHJ4-2]

Organism-specific databases

CTDi254065.
MGIiMGI:3029414. Brwd3.

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00720000108687.
HOGENOMiHOG000203884.
HOVERGENiHBG108248.
InParanoidiA2AHJ4.
KOiK11798.
OMAiXLIPRRL.
OrthoDBiEOG7N37BR.
TreeFamiTF324197.

Miscellaneous databases

ChiTaRSiBrwd3. mouse.
NextBioi403145.
PROiA2AHJ4.
SOURCEiSearch...

Gene expression databases

BgeeiA2AHJ4.
CleanExiMM_BRWD3.
ExpressionAtlasiA2AHJ4. baseline and differential.
GenevestigatoriA2AHJ4.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
2.130.10.10. 3 hits.
InterProiIPR001487. Bromodomain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
SM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50014. BROMODOMAIN_2. 2 hits.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1595-1799 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Egg and Pancreas.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1390-1799 (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Eye.

Entry informationi

Entry nameiBRWD3_MOUSE
AccessioniPrimary (citable) accession number: A2AHJ4
Secondary accession number(s): Q3TQR7
, Q3UTB6, Q640P3, Q8C7D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2007
Last sequence update: February 19, 2007
Last modified: March 31, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.