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Protein

Calmodulin-regulated spectrin-associated protein 1

Gene

Camsap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization. Specifically recognizes growing microtubule minus-ends and stabilizes microtubules. Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization. In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation. Through interaction with spectrin may regulate neurite outgrowth.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-regulated spectrin-associated protein 1
Gene namesi
Name:Camsap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3036242 Camsap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003168291 – 1581Calmodulin-regulated spectrin-associated protein 1Add BLAST1581

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216PhosphoserineBy similarity1
Modified residuei370PhosphoserineBy similarity1
Modified residuei374PhosphoserineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei511PhosphothreonineBy similarity1
Modified residuei550PhosphoserineCombined sources1
Modified residuei553PhosphoserineCombined sources1
Modified residuei560PhosphoserineBy similarity1
Modified residuei572PhosphoserineCombined sources1
Modified residuei586PhosphoserineBy similarity1
Modified residuei626PhosphoserineBy similarity1
Modified residuei718PhosphoserineBy similarity1
Modified residuei724PhosphoserineCombined sources1
Modified residuei734PhosphoserineBy similarity1
Modified residuei736PhosphoserineBy similarity1
Modified residuei1069PhosphoserineBy similarity1
Modified residuei1133PhosphoserineCombined sources1
Modified residuei1378PhosphoserineBy similarity1
Modified residuei1407PhosphoserineCombined sources1
Modified residuei1516PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2AHC3
MaxQBiA2AHC3
PaxDbiA2AHC3
PeptideAtlasiA2AHC3
PRIDEiA2AHC3

PTM databases

iPTMnetiA2AHC3
PhosphoSitePlusiA2AHC3

Expressioni

Gene expression databases

BgeeiENSMUSG00000026933
ExpressionAtlasiA2AHC3 baseline and differential
GenevisibleiA2AHC3 MM

Interactioni

Subunit structurei

Interacts with spectrin via SPTBN1; the interaction is direct. Interacts with calmodulin; calcium-dependent it prevents interaction with spectrin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230653, 2 interactors
IntActiA2AHC3, 2 interactors
MINTiA2AHC3
STRINGi10090.ENSMUSP00000109804

Structurei

3D structure databases

ProteinModelPortaliA2AHC3
SMRiA2AHC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 330Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST116
Domaini1443 – 1576CKKPROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni867 – 888Sufficient for interaction with SPTBN1By similarityAdd BLAST22
Regioni899 – 918Sufficient for interaction with calmodulinBy similarityAdd BLAST20

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili869 – 905Sequence analysisAdd BLAST37
Coiled coili1005 – 1037Sequence analysisAdd BLAST33
Coiled coili1265 – 1336Sequence analysisAdd BLAST72

Domaini

The CKK domain binds microtubules.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the CAMSAP1 family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3654 Eukaryota
ENOG4111D0B LUCA
GeneTreeiENSGT00390000010026
HOGENOMiHOG000059671
HOVERGENiHBG107572
InParanoidiA2AHC3
KOiK17493
PhylomeDBiA2AHC3
TreeFamiTF315529

Family and domain databases

Gene3Di1.10.418.10, 1 hit
3.10.20.360, 1 hit
InterProiView protein in InterPro
IPR032940 CAMSAP
IPR031372 CAMSAP_CC1
IPR022613 CAMSAP_CH
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR038209 CKK_dom_sf
IPR014797 CKK_domain
IPR011033 PRC_barrel-like_sf
PANTHERiPTHR21595 PTHR21595, 1 hit
PfamiView protein in Pfam
PF17095 CAMSAP_CC1, 1 hit
PF11971 CAMSAP_CH, 1 hit
PF08683 CAMSAP_CKK, 1 hit
SMARTiView protein in SMART
SM01051 CAMSAP_CKK, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
SSF50346 SSF50346, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS51508 CKK, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2AHC3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVDAGGRCAA EGWRRMEAPP EGADLVPLDR YDAARAKIAA NLQWICAKAY
60 70 80 90 100
GLDNIPEDLR DPFYIDQYEQ EHIKPPVIKL LLSSELYCRV CSLILKGDQV
110 120 130 140 150
ATLQGHQSVI QALSRKGIYV MESDDTPVTD ADLSQAPIKM SGHMAMVDAL
160 170 180 190 200
MMAYTVEMIS IEKVVASVKR FSTFSASKEL PYDLEDAMVF WINKVNLKMR
210 220 230 240 250
EITEKEVKLK QQPLESPAHQ KVRYRREHLS ARQSPYFPLL EDLMRDGSDG
260 270 280 290 300
AALLAVVHYY CPEQMKLDDI CLKEVPSMAD SLYNIRLLRE FSNEHLNKCF
310 320 330 340 350
YLTLEDMLYA PLVLKPNVMV FIAELFWWFE NVKPDFVQPR DIQELKDAKT
360 370 380 390 400
VLQQKSSRPP VPISNATKRS FLGSPAAMSP ADQPPSTQPL AEGSHRYHLH
410 420 430 440 450
SEEPECLGKG ASTFSPSHPL LPLRQKQQKV SQTEEIPDQR HRSNSLTRVD
460 470 480 490 500
GQPRGAIGAW PDKKNRPVSQ PTSFALHHAA SCDVDPSSGD SVSLARSISK
510 520 530 540 550
DSLASNIIHL TPQNQPHPSA GKSNGKSLLS NVNIEDEDEE LVAIIRTDVS
560 570 580 590 600
PPSPQMPRTS PQAPGLVASI RSPQRQADTL ESKPDSFYLE PLMPAVLRPA
610 620 630 640 650
KEKQITTKED ERGEGRPRTI MAKRPSEGSQ PMVRKKVSGG HGSRDLNRTF
660 670 680 690 700
TPIPCSEFAA SIDLAEVGPQ SAEATGEGQP LALGRFDTLP QGQAADGFFL
710 720 730 740 750
HVGRAEEDEG RWYVGSQSPS SHDSEPWTIL RQDSDSDVVD VEDTEQDFIG
760 770 780 790 800
EDHPVVIPRY AGEEESAKLQ EDMKVKEHED KDDASGRSSP CLSTTSQLSS
810 820 830 840 850
MSMASGSVKM TSFAERKLQR LNSCETKSST SSSQKTTPDA SESCPAPLTT
860 870 880 890 900
WRQKREQSPG RHSKDPASLL ASELVQLHMQ LEEKRRAIEA QKKKMEALSA
910 920 930 940 950
RQRLKLGKAA FLHVVKKGKA DGAPQPLRPE HFTKEFTQHN GEDLDDGTCK
960 970 980 990 1000
TEGFLVKEEQ RDLSDAQDVA FVQLHKPRDP AALHDGEKHR MISTALLEDS
1010 1020 1030 1040 1050
VGEVDVNECD LSIEKLNETI STLQQAILKI SQQQEQLLMK SPTVPTPGTK
1060 1070 1080 1090 1100
NNCQDQKIKA PVHFVEPLSP TGVPGHRKPP RLGQGRNSRS GRPAELKVPK
1110 1120 1130 1140 1150
DRQQGCSRSK TPTPSVETLP QSRSLPPSTH PRSPSDPGGE LPEKCLFDSY
1160 1170 1180 1190 1200
RLHDESNHRT FVLSSCKDAN IVSEQVNFKE GLDTSVKEAG LSSSTITGKE
1210 1220 1230 1240 1250
HTPVEEPLRS KASLIEVDLS DLKAPDEDGE VVGHESSVEL GGDSDQKPGV
1260 1270 1280 1290 1300
GFFFKDEQKA EDELAKKRAA FLLKQQRKAE EARARKQQLE AEVELKRDEA
1310 1320 1330 1340 1350
RRKAEEDRLR KEEEKARREL IKQEYLRRKQ QQALEEQGLG KPKSKPKKPR
1360 1370 1380 1390 1400
PKSVHREESY SDSGTKCSST HNLSQTHSGS SLSLASAATT EPESVYSGGT
1410 1420 1430 1440 1450
PSHRVESLEA LPILSRNPSR STDRDWETAS AASSLASVAE YTGPKLFKEP
1460 1470 1480 1490 1500
SSKSNKPIIH NAISHCCLAG KVNEPHKNSI LELEKCDANH YIILFRDAGC
1510 1520 1530 1540 1550
QFRALYCYQP DTEEIYKLTG TGPKSITKKM IDKLYKYSSD RKQFNLIPAK
1560 1570 1580
TMSVSVDALT IHNHLWQPKR PTVPKKTQTR K
Length:1,581
Mass (Da):175,887
Last modified:February 20, 2007 - v1
Checksum:i4293DE86DE3A2585
GO
Isoform 2 (identifier: A2AHC3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-221: K → KPGLEHAVMHCMLEPVDFARV
     1058-1062: IKAPV → ASPRR
     1063-1581: Missing.

Show »
Length:1,082
Mass (Da):120,300
Checksum:i4B6E596D32FCB67F
GO

Sequence cautioni

The sequence AAH54553 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti901R → L in BAC28983 (PubMed:15489334).Curated1
Sequence conflicti1482E → EE in BAC28983 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030802221K → KPGLEHAVMHCMLEPVDFAR V in isoform 2. 1 Publication1
Alternative sequenceiVSP_0308031058 – 1062IKAPV → ASPRR in isoform 2. 1 Publication5
Alternative sequenceiVSP_0308041063 – 1581Missing in isoform 2. 1 PublicationAdd BLAST519

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731682 Genomic DNA No translation available.
BC054553 mRNA Translation: AAH54553.1 Different initiation.
AK035221 mRNA Translation: BAC28983.1
RefSeqiNP_001263288.1, NM_001276359.1
NP_001263289.1, NM_001276360.1
NP_001263290.1, NM_001276361.1
UniGeneiMm.36834

Genome annotation databases

EnsembliENSMUST00000091268; ENSMUSP00000088812; ENSMUSG00000026933 [A2AHC3-1]
ENSMUST00000183461; ENSMUSP00000139028; ENSMUSG00000026933 [A2AHC3-1]
GeneIDi227634
KEGGimmu:227634

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCAMP1_MOUSE
AccessioniPrimary (citable) accession number: A2AHC3
Secondary accession number(s): Q7TQF8, Q8CBV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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