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Protein

Cytoskeleton-associated protein 5

Gene

Ckap5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoskeleton-associated protein 5
Gene namesi
Name:Ckap5Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1923036. Ckap5.

Subcellular locationi

  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity

  • Note: Detected on centrosomes during interphase. Detected on spindle poles and microtubules during mitosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20322032Cytoskeleton-associated protein 5PRO_0000364005Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481N6-acetyllysineBy similarity
Modified residuei816 – 8161PhosphoserineBy similarity
Modified residuei1469 – 14691PhosphoserineBy similarity
Modified residuei1861 – 18611PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiA2AGT5.
PaxDbiA2AGT5.
PeptideAtlasiA2AGT5.
PRIDEiA2AGT5.

PTM databases

iPTMnetiA2AGT5.
PhosphoSiteiA2AGT5.
SwissPalmiA2AGT5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040549.

Interactioni

Subunit structurei

Interacts with TACC1. Interacts with SLAIN2. Interacts with HNRNPA2B1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217741. 32 interactions.
IntActiA2AGT5. 30 interactions.
STRINGi10090.ENSMUSP00000097303.

Structurei

3D structure databases

ProteinModelPortaliA2AGT5.
SMRiA2AGT5. Positions 852-1080.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati159 – 19739HEAT 1Sequence analysisAdd
BLAST
Repeati356 – 39439HEAT 2Sequence analysisAdd
BLAST
Repeati434 – 47239HEAT 3Sequence analysisAdd
BLAST
Repeati750 – 78839HEAT 4Sequence analysisAdd
BLAST
Repeati855 – 89339HEAT 5Sequence analysisAdd
BLAST
Repeati936 – 97439HEAT 6Sequence analysisAdd
BLAST
Repeati1013 – 105139HEAT 7Sequence analysisAdd
BLAST
Repeati1284 – 132239HEAT 8Sequence analysisAdd
BLAST
Repeati1324 – 135734HEAT 9Sequence analysisAdd
BLAST
Repeati1361 – 139939HEAT 10Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the TOG/XMAP215 family.Sequence analysis
Contains 10 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1820. Eukaryota.
ENOG410XPTW. LUCA.
HOVERGENiHBG050955.
InParanoidiA2AGT5.
KOiK16803.
PhylomeDBiA2AGT5.

Family and domain databases

Gene3Di1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
IPR021133. HEAT_type_2.
[Graphical view]
PfamiPF12348. CLASP_N. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 10 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: A2AGT5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDDSEWLKL PVDQKCEHKL WKARLSGYEE ALKIFQKIKD EKSPEWSKYL
60 70 80 90 100
GLIKKFVTDS NAVVQLKGLE AALVYVENAH VAGKTTGEVV SGVVSKVFNQ
110 120 130 140 150
PKAKAKELGI EICLMYVEIE KGESVQEELL KGLDNKNPKI IVACIETLRK
160 170 180 190 200
ALSEFGSKII SLKPIIKVLP KLFESRDKAV RDEAKLFAIE IYRWNRDAVK
210 220 230 240 250
HTLQNINSVQ LKELEEEWVK LPTGAPKPSR FLRSQQELEA KLEQQQSAGG
260 270 280 290 300
DAEGGGDDGD EVPQVDAYEL LDAVEILSKL PKDFYDKIEA KKWQERKEAL
310 320 330 340 350
EAVEVLVKNP KLEAGDYADL VKALKKVVGK DTNVMLVALA AKCLTGLAVG
360 370 380 390 400
LRKKFGQYAG HVVPTILEKF KEKKPQVVQA LQEAIDAIFL TTTLQNISED
410 420 430 440 450
VLAVMDNKNP TIKQQTSLFI ARSFRHCTSS TLPKSLLKPF CAALLKHIND
460 470 480 490 500
SAPEVRDAAF EALGTALKVV GEKSVNPFLA DVDKLKLDRI KECSEKVELV
510 520 530 540 550
HGKKSGLATE KKESKPLPGR AAASGAAGDK DTKDVSGPKP GPLKKTPTAK
560 570 580 590 600
AGGPSKKGKT TAPGGSASAG TKNKKGLETK EIVEPELSIE VCEEKASAVL
610 620 630 640 650
PPTCIQLLDS SNWKERLACM EEFQKAVELM ERTEMPCQAL VKMLAKKPGW
660 670 680 690 700
KETNFQVMQM KLHIVALIAQ KGNFSKTSAQ IVLDGLVDKI GDVKCGNNAK
710 720 730 740 750
EAMTAIAEAC MLPWTAEQVM SMAFSQKNPK NQSETLNWLS NAIKEFGFSE
760 770 780 790 800
LNVKAFISNV KTALAATNPA VRTSAITLLG VMYLYVGPSL RMIFEDEKPA
810 820 830 840 850
LLSQIDAEFQ KMQGQSPPAP TRGIAKHSTS ATDEGEDGEE PGEGGNDVVD
860 870 880 890 900
LLPRIEISDK ITSELVSKIG DKNWKIRKEG LDEVAGIINE AKFIQPNIGE
910 920 930 940 950
LPTALKGRLN DSNKILVQQT LNILQQLAVA MGANIRQHVK NLGIPVITVL
960 970 980 990 1000
GDSKNNVRAA ALATVNAWAE QTGMKEWLEG EDLSEELKKE NPFLRQELLG
1010 1020 1030 1040 1050
WLAEKLPTLR STPTDLILCV PHLYSCLKDR NGDVRKKAQD ALPFFMMHLG
1060 1070 1080 1090 1100
YEKMAKATGK LKPTSKDQVL AMLEKAKANM PSKPAAPAKA MSKPMGGSAP
1110 1120 1130 1140 1150
AKTQPIPAPV EDSVSSTIEA KPDLKKAKAP GVSSKAKSVQ GKKVPSKTTL
1160 1170 1180 1190 1200
KEDDDKSGPI FIVVPNGKEQ RMRDEKGLKV LKWNFTTPRD EYIEQLKTQM
1210 1220 1230 1240 1250
STCVAKWLQD EMFHSDFQHH NKALAVMVDH LESEKDGVIS CLDLILKWLT
1260 1270 1280 1290 1300
LRFFDTNTSV LMKALEYLKL LFTLLSEEEY HLTENEASSF IPYLILKVGE
1310 1320 1330 1340 1350
PKDVIRKDVR AILNRMCLVY PASKMFPFIM EGTKSKNSKQ RAECLEELGC
1360 1370 1380 1390 1400
LIESYGMNVC QPTPGKALKE IAIHIGDRDN AVRNAALNTI VTVYNVHGDQ
1410 1420 1430 1440 1450
VFKLIGNLSE KDMSMLEERI KRSAKRPSAA PVKQAEEKPQ RTQNINSNAN
1460 1470 1480 1490 1500
MLRKGPAEDM SSKLNQARSL SGHPEAAQMV RREFQLDLDE IENDNGTVRC
1510 1520 1530 1540 1550
EMPELVQHKL DDIFEPVLIP EPKIRAVSPH FDDMHSNTAS TINFIISQVA
1560 1570 1580 1590 1600
SGDINTSIQA LTQIDEVLRQ EDKAEAMSGH IDQFLIATFM QLRLIYSTHM
1610 1620 1630 1640 1650
ADEKLDKDEI IKLYSCIIGN MISLFQIESL AREASTGVLK DLMHGLITLM
1660 1670 1680 1690 1700
LDSRIEDLEE GQQVIRSVNL LVVKVLEKSD QTNILSALLV LLQDSLLATA
1710 1720 1730 1740 1750
SSPKFSELVM KCLWRMVRLL PDTINSINLD RILLDIHIFM KVFPKEKLKQ
1760 1770 1780 1790 1800
CKSEFPIRTL KTLLHTLCKL KGPKILDHLT MIDNKNESEL EAHLCRMMKH
1810 1820 1830 1840 1850
SMDQTGSKSD KETEKGASRI DEKSSKAKVN DFLAEIFKKI GSKENTKEGL
1860 1870 1880 1890 1900
AELYEYKKKY SDTDIEPFLK NSSQFFQSYV ERGLRVIEME RESKGRIPTS
1910 1920 1930 1940 1950
TGISPQMEVT CVPTPTSTVS SLGNTNGEEV GPSVYLERLK ILRQRCGLDN
1960 1970 1980 1990 2000
TKQDDRPPLT SLLSKPAIPP VASSTDMLHS KLSQLRESRE QHQHSDLDSN
2010 2020 2030
QTHSAGTMTS SSSTTNIDDL KKRLERIKSS RK
Length:2,032
Mass (Da):225,635
Last modified:February 20, 2007 - v1
Checksum:i28FD8628C6F678CB
GO
Isoform 2 (identifier: A2AGT5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1564-1623: Missing.

Note: No experimental confirmation available. Gene prediction based on similarity to human ortholog.
Show »
Length:1,972
Mass (Da):218,707
Checksum:iF33AAA7B32F32540
GO
Isoform 31 Publication (identifier: A2AGT5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1903-1923: Missing.

Show »
Length:2,011
Mass (Da):223,518
Checksum:i0F8E07DCC94668C5
GO

Sequence cautioni

The sequence AAH17140 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51S → C in BAE38422 (PubMed:16141072).Curated
Sequence conflicti19 – 191K → N in BAB29779 (PubMed:16141072).Curated
Sequence conflicti111 – 1111E → K in BAB29779 (PubMed:16141072).Curated
Sequence conflicti174 – 1741E → G in BAE38422 (PubMed:16141072).Curated
Sequence conflicti227 – 2271K → R in BAB29779 (PubMed:16141072).Curated
Sequence conflicti522 – 5221A → T in BAB29779 (PubMed:16141072).Curated
Sequence conflicti581 – 5811E → Q in BAB29779 (PubMed:16141072).Curated
Sequence conflicti675 – 6751S → F in BAB29779 (PubMed:16141072).Curated
Sequence conflicti709 – 7091A → G in BAB29779 (PubMed:16141072).Curated
Sequence conflicti836 – 8361E → V in BAE29125 (PubMed:16141072).Curated
Sequence conflicti981 – 9811E → A in AAH89032 (PubMed:15489334).Curated
Sequence conflicti1028 – 10281K → E in AAH89032 (PubMed:15489334).Curated
Sequence conflicti1028 – 10281K → E in AAH96422 (PubMed:15489334).Curated
Sequence conflicti1028 – 10281K → E in AAI38335 (PubMed:15489334).Curated
Sequence conflicti1717 – 17171V → F in AAH96422 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1564 – 162360Missing in isoform 2. CuratedVSP_053003Add
BLAST
Alternative sequencei1903 – 192321Missing in isoform 3. 1 PublicationVSP_053004Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL691489 Genomic DNA. No translation available.
BC017140 mRNA. Translation: AAH17140.1. Sequence problems.
BC089032 mRNA. Translation: AAH89032.1.
BC096422 mRNA. Translation: AAH96422.1.
BC138334 mRNA. Translation: AAI38335.1.
AK015282 mRNA. Translation: BAB29779.1.
AK149854 mRNA. Translation: BAE29125.1.
AK165862 mRNA. Translation: BAE38422.1.
CCDSiCCDS16433.1. [A2AGT5-3]
CCDS50640.1. [A2AGT5-1]
RefSeqiNP_001159461.1. NM_001165989.1.
NP_083713.2. NM_029437.2.
UniGeneiMm.168478.

Genome annotation databases

GeneIDi75786.
KEGGimmu:75786.
UCSCiuc008kwd.1. mouse. [A2AGT5-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL691489 Genomic DNA. No translation available.
BC017140 mRNA. Translation: AAH17140.1. Sequence problems.
BC089032 mRNA. Translation: AAH89032.1.
BC096422 mRNA. Translation: AAH96422.1.
BC138334 mRNA. Translation: AAI38335.1.
AK015282 mRNA. Translation: BAB29779.1.
AK149854 mRNA. Translation: BAE29125.1.
AK165862 mRNA. Translation: BAE38422.1.
CCDSiCCDS16433.1. [A2AGT5-3]
CCDS50640.1. [A2AGT5-1]
RefSeqiNP_001159461.1. NM_001165989.1.
NP_083713.2. NM_029437.2.
UniGeneiMm.168478.

3D structure databases

ProteinModelPortaliA2AGT5.
SMRiA2AGT5. Positions 852-1080.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217741. 32 interactions.
IntActiA2AGT5. 30 interactions.
STRINGi10090.ENSMUSP00000097303.

PTM databases

iPTMnetiA2AGT5.
PhosphoSiteiA2AGT5.
SwissPalmiA2AGT5.

Proteomic databases

MaxQBiA2AGT5.
PaxDbiA2AGT5.
PeptideAtlasiA2AGT5.
PRIDEiA2AGT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi75786.
KEGGimmu:75786.
UCSCiuc008kwd.1. mouse. [A2AGT5-3]

Organism-specific databases

CTDi9793.
MGIiMGI:1923036. Ckap5.

Phylogenomic databases

eggNOGiKOG1820. Eukaryota.
ENOG410XPTW. LUCA.
HOVERGENiHBG050955.
InParanoidiA2AGT5.
KOiK16803.
PhylomeDBiA2AGT5.

Miscellaneous databases

PROiA2AGT5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040549.

Family and domain databases

Gene3Di1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
IPR021133. HEAT_type_2.
[Graphical view]
PfamiPF12348. CLASP_N. 3 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 10 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCKAP5_MOUSE
AccessioniPrimary (citable) accession number: A2AGT5
Secondary accession number(s): A0PJ77
, A2AGT6, B2RRC4, Q0VGR0, Q3TML9, Q3UDY6, Q4VAE7, Q9CUN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 20, 2007
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.