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A2AGH6

- MED12_MOUSE

UniProt

A2AGH6 - MED12_MOUSE

Protein

Mediator of RNA polymerase II transcription subunit 12

Gene

Med12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway By similarity.By similarity

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. protein binding Source: IntAct
    3. receptor activity Source: Ensembl
    4. RNA polymerase II distal enhancer sequence-specific DNA binding Source: MGI
    5. RNA polymerase II transcription cofactor activity Source: Ensembl
    6. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI
    7. transcription coactivator activity Source: Ensembl
    8. transcription factor binding Source: MGI

    GO - Biological processi

    1. androgen receptor signaling pathway Source: Ensembl
    2. axis elongation involved in somitogenesis Source: MGI
    3. canonical Wnt signaling pathway Source: MGI
    4. heart development Source: MGI
    5. negative regulation of Wnt signaling pathway Source: Ensembl
    6. neural tube closure Source: MGI
    7. oligodendrocyte development Source: MGI
    8. Schwann cell development Source: MGI
    9. stem cell maintenance Source: MGI
    10. transcription initiation from RNA polymerase II promoter Source: Ensembl
    11. Wnt signaling pathway, planar cell polarity pathway Source: MGI

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_206529. Generic Transcription Pathway.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 12
    Alternative name(s):
    Mediator complex subunit 12
    OPA-containing protein
    Thyroid hormone receptor-associated protein complex 230 kDa component
    Short name:
    Trap230
    Trinucleotide repeat-containing gene 11 protein
    Gene namesi
    Name:Med12
    Synonyms:Kiaa0192, Mopa, Tnrc11, Trap230
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1926212. Med12.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: MGI
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21902190Mediator of RNA polymerase II transcription subunit 12PRO_0000312957Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei80 – 801N6-acetyllysine1 Publication
    Modified residuei166 – 1661PhosphotyrosineBy similarity
    Modified residuei636 – 6361PhosphoserineBy similarity
    Modified residuei1259 – 12591PhosphoserineBy similarity
    Modified residuei1270 – 12701PhosphoserineBy similarity
    Modified residuei1800 – 18001N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiA2AGH6.
    PaxDbiA2AGH6.
    PRIDEiA2AGH6.

    PTM databases

    PhosphoSiteiA2AGH6.

    Expressioni

    Gene expression databases

    BgeeiA2AGH6.
    GenevestigatoriA2AGH6.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TGFBR2P371733EBI-5744969,EBI-296151From a different organism.

    Protein-protein interaction databases

    BioGridi208491. 1 interaction.
    DIPiDIP-59233N.
    IntActiA2AGH6. 5 interactions.
    MINTiMINT-4105605.

    Structurei

    3D structure databases

    ProteinModelPortaliA2AGH6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1617 – 2063447Interaction with CTNNB1 and GLI3By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 12 family.Curated

    Phylogenomic databases

    eggNOGiNOG319131.
    GeneTreeiENSGT00440000037505.
    HOGENOMiHOG000231423.
    HOVERGENiHBG052447.
    InParanoidiA2AGH6.
    KOiK15162.
    OMAiTVDMQSN.
    TreeFamiTF324178.

    Family and domain databases

    InterProiIPR019035. Mediator_Med12.
    IPR021989. Mediator_Med12_catenin-bd.
    IPR021990. Mediator_Med12_LCEWAV.
    [Graphical view]
    PfamiPF09497. Med12. 1 hit.
    PF12145. Med12-LCEWAV. 1 hit.
    PF12144. Med12-PQL. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A2AGH6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAFGILSYE HRPLKRLRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA     50
    VSGDEHGSAK NVNFNPAKIS SNFSSIIAEK LRCNTLSDTG RRKSLMNQKD 100
    NFWLVTARSQ SAINTWFTDL AGTKPLTHLA KKVPIFSKKE EVFGYLAKYT 150
    VPVMRAAWLI KMTCAYYAAM SETKVKKKNT ADPFTEWTQI ITKYLWEQLQ 200
    KMAEYYRPGP AGSGGCGSTI GPLPHDVEMA IRQWDYNEKL ALFMFQDGML 250
    DRHEFLTWVL ECFEKIRPGE DELLKLLLPL LLRYSGEFVQ SAYLSRRLAY 300
    FCTRRLALQL DGVSSHSSHV IAAQSTSSLP TTPAPQPPTS STPSTPFSDL 350
    LMCPQHRPLV FGLSCILQTI LLCCPSALVW HYSLTDSRIK TGSPLDHLPI 400
    APSNLPMPEG NSAFTQQVRA KLREIEQQIK ERGQAVEVRW SFDKCQEATA 450
    GFTIGRVLHT LEVLDSHSFE RSDFSNSLDS LCNRIFGLGP SKDGHEISSD 500
    DDAVVSLLCE WAVSCKRSGR HRAMVVAKLL EKRQAEIEAE RCGESEAADE 550
    KGSVASGSLS APSAPIFQDV LLQFLDTQAP MLTDPRSESE RVEFFNLVLL 600
    FCELIRHDVF SHNMYTCTLI SRGDLAFGAP GPRPPSPFDD PTDDPERKEA 650
    EGSSSSKLED PGLSESMDID PSSSVLFEDM EKPDFSLFSP TMPCEGKGSP 700
    SPEKPDVEKE VKPPAKEKIE GTLGILYDQP RHVQYATHFP IPQEESCSHE 750
    CNQRLVVLFG VGKQRDDARH AIKKITKDIL KVLNRKGTAE TDQLAPIVPL 800
    NPGDLTFLGG EDGQKRRRNR PEAFPTAEDI FAKFQHLSHY DQHQVTAQVS 850
    RNVLEQITSF ALGMSYHLPL VQHVQFIFDL MEYSLSISGL IDFAIQLLNE 900
    LSVVEAELLL KSSDLVGSYT TSLCLCIVAV LRHYHACLIL NQDQMAQVFE 950
    GLCGVVKHGM NRSDGSSAER CILAYLYDLY TSCSHLKSKF GELFSDFCSK 1000
    VKNTIYCNVE PSESNMRWAP EFMIDTLENP AAHTFTYTGL GKSLSENPAN 1050
    RYSFVCNALM HVCVGHHDPD RVNDIAILCA ELTGYCKSLS AEWLGVLKAL 1100
    CCSSNNGTCG FNDLLCNVDV SDLSFHDSLA TFVAILIARQ CLLLEDLIRC 1150
    AAIPSLLNAA CSEQDSEPGA RLTCRILLHL FKTPQLNPCQ SDGNKPTVGI 1200
    RSSCDRHLLA ASQNRIVDGA VFAVLKAVFV LGDAELKGSG FTVPGGTEEL 1250
    PEEEGGGGSS GRRQGGRNIS VETASLDVYA KYVLRSICQQ EWVGERCLKS 1300
    LCEDSNDLQD PVLSSAQAQR LMQLICYPHR LLDNEDGENP QRQRIKRILK 1350
    NLDQWTMRQS SLELQLMIKQ TPNTEMNSLL ENIAKATIEV FQQSAETGSS 1400
    SGSTASNMPS SSKTKPVLSS LERSGVWLVA PLIAKLPTSV QGHVLKAAGE 1450
    ELEKGQHLGS SSRKERDRQK QKSMSLLSQQ PFLSLVLTCL KGQDEQREGL 1500
    LASLHSQVHQ IVINWRENQY LDDCKPKQLM HEALKLRLNL VGGMFDTVQR 1550
    STQQTTEWAQ LLLEIIISGT VDMQSNNELF TTVLDMLSVL INGTLAADMS 1600
    SISQGSMEEN KRAYMNLVKK LQKDLGERQS DSLEKVHQLL PLPKQNRDVI 1650
    TCEPQGSLID TKGNKIAGFD SIFKKEGLQV STKQKISPWE LFEGLKPSTA 1700
    PLSWAWFGTV RVDRRVARGE EQQRLLLYHT HLRPRPRAYY LEPLPLPPED 1750
    EEPPAPALLE PEKKAPEPPK TDKPGAAPPS TEERKKKSTK GKKRSQPATK 1800
    NEDYGMGPGR SGPYGVTVPP DLLHHANPGS ISHLSYRQSS MGLYTQNQPL 1850
    PAGGPRVDPY RPVRLPMQKL PTRPTYPGVL PTTMSTVMGL EPSSYKTSVY 1900
    RQQQPTVPQG QRLRQQLQAK IQSQGMLGQS SVHQMTPSSS YGLQTSQGYT 1950
    SYVSHVGLQQ HTGPAGTMVP PSYSSQPYQS THPSTNPTLV DPTRHLQQRP 2000
    SGYVHQQAPT YGHGLTSTQR FSHQTLQQTP MMGTMTPLSA QGVQAGVRST 2050
    SILPEQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQYH IRQQQQQQQM 2100
    LRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQP HQQQQQAAPP QPQPQSQPQF 2150
    QRQGLQQTQQ QQQTAALVRQ LQQQLSNTQP QPSTNIFGRY 2190
    Length:2,190
    Mass (Da):244,561
    Last modified:February 20, 2007 - v1
    Checksum:i146E05DCCFF38DB4
    GO
    Isoform 2 (identifier: A2AGH6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1919-1921: Missing.
         1946-1946: S → SQLSSPSL
         1966-1990: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:2,169
    Mass (Da):242,303
    Checksum:i8BE79B8DEFD56844
    GO
    Isoform 3 (identifier: A2AGH6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1321: Missing.
         1919-1921: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:866
    Mass (Da):97,885
    Checksum:i4A782193143882B0
    GO

    Sequence cautioni

    The sequence AAC83164.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti674 – 6741S → T in AAC83164. (PubMed:15489334)Curated
    Sequence conflicti1597 – 15971A → T in BAD90141. 1 PublicationCurated
    Sequence conflicti2171 – 21711L → I in AAC83164. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 13211321Missing in isoform 3. 1 PublicationVSP_029977Add
    BLAST
    Alternative sequencei1919 – 19213Missing in isoform 2 and isoform 3. 2 PublicationsVSP_029978
    Alternative sequencei1946 – 19461S → SQLSSPSL in isoform 2. 1 PublicationVSP_029979
    Alternative sequencei1966 – 199025Missing in isoform 2. 1 PublicationVSP_029980Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL683892 Genomic DNA. Translation: CAM24445.1.
    AL683892 Genomic DNA. Translation: CAM24446.1.
    BC057085 mRNA. Translation: AAH57085.1.
    BC057119 mRNA. Translation: AAH57119.1.
    AF071310 mRNA. Translation: AAC83164.1. Different initiation.
    AK220216 mRNA. Translation: BAD90141.1.
    CCDSiCCDS41078.1. [A2AGH6-1]
    RefSeqiNP_067496.2. NM_021521.2. [A2AGH6-1]
    UniGeneiMm.20873.
    Mm.490933.

    Genome annotation databases

    EnsembliENSMUST00000087948; ENSMUSP00000085260; ENSMUSG00000079487. [A2AGH6-1]
    ENSMUST00000087956; ENSMUSP00000085269; ENSMUSG00000079487. [A2AGH6-2]
    GeneIDi59024.
    KEGGimmu:59024.
    UCSCiuc009txe.1. mouse. [A2AGH6-1]
    uc009txf.1. mouse. [A2AGH6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL683892 Genomic DNA. Translation: CAM24445.1 .
    AL683892 Genomic DNA. Translation: CAM24446.1 .
    BC057085 mRNA. Translation: AAH57085.1 .
    BC057119 mRNA. Translation: AAH57119.1 .
    AF071310 mRNA. Translation: AAC83164.1 . Different initiation.
    AK220216 mRNA. Translation: BAD90141.1 .
    CCDSi CCDS41078.1. [A2AGH6-1 ]
    RefSeqi NP_067496.2. NM_021521.2. [A2AGH6-1 ]
    UniGenei Mm.20873.
    Mm.490933.

    3D structure databases

    ProteinModelPortali A2AGH6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208491. 1 interaction.
    DIPi DIP-59233N.
    IntActi A2AGH6. 5 interactions.
    MINTi MINT-4105605.

    PTM databases

    PhosphoSitei A2AGH6.

    Proteomic databases

    MaxQBi A2AGH6.
    PaxDbi A2AGH6.
    PRIDEi A2AGH6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000087948 ; ENSMUSP00000085260 ; ENSMUSG00000079487 . [A2AGH6-1 ]
    ENSMUST00000087956 ; ENSMUSP00000085269 ; ENSMUSG00000079487 . [A2AGH6-2 ]
    GeneIDi 59024.
    KEGGi mmu:59024.
    UCSCi uc009txe.1. mouse. [A2AGH6-1 ]
    uc009txf.1. mouse. [A2AGH6-2 ]

    Organism-specific databases

    CTDi 9968.
    MGIi MGI:1926212. Med12.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG319131.
    GeneTreei ENSGT00440000037505.
    HOGENOMi HOG000231423.
    HOVERGENi HBG052447.
    InParanoidi A2AGH6.
    KOi K15162.
    OMAi TVDMQSN.
    TreeFami TF324178.

    Enzyme and pathway databases

    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_206529. Generic Transcription Pathway.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    NextBioi 314592.
    PROi A2AGH6.
    SOURCEi Search...

    Gene expression databases

    Bgeei A2AGH6.
    Genevestigatori A2AGH6.

    Family and domain databases

    InterProi IPR019035. Mediator_Med12.
    IPR021989. Mediator_Med12_catenin-bd.
    IPR021990. Mediator_Med12_LCEWAV.
    [Graphical view ]
    Pfami PF09497. Med12. 1 hit.
    PF12145. Med12-LCEWAV. 1 hit.
    PF12144. Med12-PQL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-2190 (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Association of an X-chromosome dodecamer insertional variant allele with mental retardation."
      Philibert R.A., King B.H., Cook E.H., Lee Y.-H., Stubblefield B., Damschroder-Williams P., Dea C., Palotie A., Tengstrom C., Martin B.M., Ginns E.I.
      Mol. Psychiatry 3:303-309(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-2190 (ISOFORM 2).
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1045-2190 (ISOFORM 1).
      Tissue: Spleen.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-80 AND LYS-1800, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiMED12_MOUSE
    AccessioniPrimary (citable) accession number: A2AGH6
    Secondary accession number(s): A2AGH7
    , O88542, Q571H3, Q6PGB4, Q6PGD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3