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A2AGH6

- MED12_MOUSE

UniProt

A2AGH6 - MED12_MOUSE

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Protein

Mediator of RNA polymerase II transcription subunit 12

Gene

Med12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).By similarity

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. receptor activity Source: Ensembl
  3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: MGI
  4. RNA polymerase II transcription cofactor activity Source: Ensembl
  5. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI
  6. transcription coactivator activity Source: Ensembl
  7. transcription factor binding Source: MGI

GO - Biological processi

  1. androgen receptor signaling pathway Source: Ensembl
  2. axis elongation involved in somitogenesis Source: MGI
  3. canonical Wnt signaling pathway Source: MGI
  4. heart development Source: MGI
  5. negative regulation of Wnt signaling pathway Source: Ensembl
  6. neural tube closure Source: MGI
  7. oligodendrocyte development Source: MGI
  8. Schwann cell development Source: MGI
  9. stem cell maintenance Source: MGI
  10. transcription initiation from RNA polymerase II promoter Source: Ensembl
  11. Wnt signaling pathway, planar cell polarity pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 12
Alternative name(s):
Mediator complex subunit 12
OPA-containing protein
Thyroid hormone receptor-associated protein complex 230 kDa component
Short name:
Trap230
Trinucleotide repeat-containing gene 11 protein
Gene namesi
Name:Med12
Synonyms:Kiaa0192, Mopa, Tnrc11, Trap230
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1926212. Med12.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21902190Mediator of RNA polymerase II transcription subunit 12PRO_0000312957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801N6-acetyllysine1 Publication
Modified residuei166 – 1661PhosphotyrosineBy similarity
Modified residuei636 – 6361PhosphoserineBy similarity
Modified residuei1259 – 12591PhosphoserineBy similarity
Modified residuei1270 – 12701PhosphoserineBy similarity
Modified residuei1800 – 18001N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiA2AGH6.
PaxDbiA2AGH6.
PRIDEiA2AGH6.

PTM databases

PhosphoSiteiA2AGH6.

Expressioni

Gene expression databases

BgeeiA2AGH6.
ExpressionAtlasiA2AGH6. baseline and differential.
GenevestigatoriA2AGH6.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TGFBR2P371733EBI-5744969,EBI-296151From a different organism.

Protein-protein interaction databases

BioGridi208491. 1 interaction.
DIPiDIP-59233N.
IntActiA2AGH6. 5 interactions.
MINTiMINT-4105605.

Structurei

3D structure databases

ProteinModelPortaliA2AGH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1617 – 2063447Interaction with CTNNB1 and GLI3By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 12 family.Curated

Phylogenomic databases

eggNOGiNOG319131.
GeneTreeiENSGT00440000037505.
HOGENOMiHOG000231423.
HOVERGENiHBG052447.
InParanoidiA2AGH6.
KOiK15162.
OMAiTVDMQSN.
TreeFamiTF324178.

Family and domain databases

InterProiIPR019035. Mediator_Med12.
IPR021989. Mediator_Med12_catenin-bd.
IPR021990. Mediator_Med12_LCEWAV.
[Graphical view]
PfamiPF09497. Med12. 1 hit.
PF12145. Med12-LCEWAV. 1 hit.
PF12144. Med12-PQL. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A2AGH6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAFGILSYE HRPLKRLRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA
60 70 80 90 100
VSGDEHGSAK NVNFNPAKIS SNFSSIIAEK LRCNTLSDTG RRKSLMNQKD
110 120 130 140 150
NFWLVTARSQ SAINTWFTDL AGTKPLTHLA KKVPIFSKKE EVFGYLAKYT
160 170 180 190 200
VPVMRAAWLI KMTCAYYAAM SETKVKKKNT ADPFTEWTQI ITKYLWEQLQ
210 220 230 240 250
KMAEYYRPGP AGSGGCGSTI GPLPHDVEMA IRQWDYNEKL ALFMFQDGML
260 270 280 290 300
DRHEFLTWVL ECFEKIRPGE DELLKLLLPL LLRYSGEFVQ SAYLSRRLAY
310 320 330 340 350
FCTRRLALQL DGVSSHSSHV IAAQSTSSLP TTPAPQPPTS STPSTPFSDL
360 370 380 390 400
LMCPQHRPLV FGLSCILQTI LLCCPSALVW HYSLTDSRIK TGSPLDHLPI
410 420 430 440 450
APSNLPMPEG NSAFTQQVRA KLREIEQQIK ERGQAVEVRW SFDKCQEATA
460 470 480 490 500
GFTIGRVLHT LEVLDSHSFE RSDFSNSLDS LCNRIFGLGP SKDGHEISSD
510 520 530 540 550
DDAVVSLLCE WAVSCKRSGR HRAMVVAKLL EKRQAEIEAE RCGESEAADE
560 570 580 590 600
KGSVASGSLS APSAPIFQDV LLQFLDTQAP MLTDPRSESE RVEFFNLVLL
610 620 630 640 650
FCELIRHDVF SHNMYTCTLI SRGDLAFGAP GPRPPSPFDD PTDDPERKEA
660 670 680 690 700
EGSSSSKLED PGLSESMDID PSSSVLFEDM EKPDFSLFSP TMPCEGKGSP
710 720 730 740 750
SPEKPDVEKE VKPPAKEKIE GTLGILYDQP RHVQYATHFP IPQEESCSHE
760 770 780 790 800
CNQRLVVLFG VGKQRDDARH AIKKITKDIL KVLNRKGTAE TDQLAPIVPL
810 820 830 840 850
NPGDLTFLGG EDGQKRRRNR PEAFPTAEDI FAKFQHLSHY DQHQVTAQVS
860 870 880 890 900
RNVLEQITSF ALGMSYHLPL VQHVQFIFDL MEYSLSISGL IDFAIQLLNE
910 920 930 940 950
LSVVEAELLL KSSDLVGSYT TSLCLCIVAV LRHYHACLIL NQDQMAQVFE
960 970 980 990 1000
GLCGVVKHGM NRSDGSSAER CILAYLYDLY TSCSHLKSKF GELFSDFCSK
1010 1020 1030 1040 1050
VKNTIYCNVE PSESNMRWAP EFMIDTLENP AAHTFTYTGL GKSLSENPAN
1060 1070 1080 1090 1100
RYSFVCNALM HVCVGHHDPD RVNDIAILCA ELTGYCKSLS AEWLGVLKAL
1110 1120 1130 1140 1150
CCSSNNGTCG FNDLLCNVDV SDLSFHDSLA TFVAILIARQ CLLLEDLIRC
1160 1170 1180 1190 1200
AAIPSLLNAA CSEQDSEPGA RLTCRILLHL FKTPQLNPCQ SDGNKPTVGI
1210 1220 1230 1240 1250
RSSCDRHLLA ASQNRIVDGA VFAVLKAVFV LGDAELKGSG FTVPGGTEEL
1260 1270 1280 1290 1300
PEEEGGGGSS GRRQGGRNIS VETASLDVYA KYVLRSICQQ EWVGERCLKS
1310 1320 1330 1340 1350
LCEDSNDLQD PVLSSAQAQR LMQLICYPHR LLDNEDGENP QRQRIKRILK
1360 1370 1380 1390 1400
NLDQWTMRQS SLELQLMIKQ TPNTEMNSLL ENIAKATIEV FQQSAETGSS
1410 1420 1430 1440 1450
SGSTASNMPS SSKTKPVLSS LERSGVWLVA PLIAKLPTSV QGHVLKAAGE
1460 1470 1480 1490 1500
ELEKGQHLGS SSRKERDRQK QKSMSLLSQQ PFLSLVLTCL KGQDEQREGL
1510 1520 1530 1540 1550
LASLHSQVHQ IVINWRENQY LDDCKPKQLM HEALKLRLNL VGGMFDTVQR
1560 1570 1580 1590 1600
STQQTTEWAQ LLLEIIISGT VDMQSNNELF TTVLDMLSVL INGTLAADMS
1610 1620 1630 1640 1650
SISQGSMEEN KRAYMNLVKK LQKDLGERQS DSLEKVHQLL PLPKQNRDVI
1660 1670 1680 1690 1700
TCEPQGSLID TKGNKIAGFD SIFKKEGLQV STKQKISPWE LFEGLKPSTA
1710 1720 1730 1740 1750
PLSWAWFGTV RVDRRVARGE EQQRLLLYHT HLRPRPRAYY LEPLPLPPED
1760 1770 1780 1790 1800
EEPPAPALLE PEKKAPEPPK TDKPGAAPPS TEERKKKSTK GKKRSQPATK
1810 1820 1830 1840 1850
NEDYGMGPGR SGPYGVTVPP DLLHHANPGS ISHLSYRQSS MGLYTQNQPL
1860 1870 1880 1890 1900
PAGGPRVDPY RPVRLPMQKL PTRPTYPGVL PTTMSTVMGL EPSSYKTSVY
1910 1920 1930 1940 1950
RQQQPTVPQG QRLRQQLQAK IQSQGMLGQS SVHQMTPSSS YGLQTSQGYT
1960 1970 1980 1990 2000
SYVSHVGLQQ HTGPAGTMVP PSYSSQPYQS THPSTNPTLV DPTRHLQQRP
2010 2020 2030 2040 2050
SGYVHQQAPT YGHGLTSTQR FSHQTLQQTP MMGTMTPLSA QGVQAGVRST
2060 2070 2080 2090 2100
SILPEQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQYH IRQQQQQQQM
2110 2120 2130 2140 2150
LRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQP HQQQQQAAPP QPQPQSQPQF
2160 2170 2180 2190
QRQGLQQTQQ QQQTAALVRQ LQQQLSNTQP QPSTNIFGRY
Length:2,190
Mass (Da):244,561
Last modified:February 20, 2007 - v1
Checksum:i146E05DCCFF38DB4
GO
Isoform 2 (identifier: A2AGH6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1919-1921: Missing.
     1946-1946: S → SQLSSPSL
     1966-1990: Missing.

Note: No experimental confirmation available.

Show »
Length:2,169
Mass (Da):242,303
Checksum:i8BE79B8DEFD56844
GO
Isoform 3 (identifier: A2AGH6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1321: Missing.
     1919-1921: Missing.

Note: No experimental confirmation available.

Show »
Length:866
Mass (Da):97,885
Checksum:i4A782193143882B0
GO

Sequence cautioni

The sequence AAC83164.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti674 – 6741S → T in AAC83164. (PubMed:15489334)Curated
Sequence conflicti1597 – 15971A → T in BAD90141. 1 PublicationCurated
Sequence conflicti2171 – 21711L → I in AAC83164. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 13211321Missing in isoform 3. 1 PublicationVSP_029977Add
BLAST
Alternative sequencei1919 – 19213Missing in isoform 2 and isoform 3. 2 PublicationsVSP_029978
Alternative sequencei1946 – 19461S → SQLSSPSL in isoform 2. 1 PublicationVSP_029979
Alternative sequencei1966 – 199025Missing in isoform 2. 1 PublicationVSP_029980Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683892 Genomic DNA. Translation: CAM24445.1.
AL683892 Genomic DNA. Translation: CAM24446.1.
BC057085 mRNA. Translation: AAH57085.1.
BC057119 mRNA. Translation: AAH57119.1.
AF071310 mRNA. Translation: AAC83164.1. Different initiation.
AK220216 mRNA. Translation: BAD90141.1.
CCDSiCCDS41078.1. [A2AGH6-1]
RefSeqiNP_067496.2. NM_021521.2. [A2AGH6-1]
UniGeneiMm.20873.
Mm.490933.

Genome annotation databases

EnsembliENSMUST00000087948; ENSMUSP00000085260; ENSMUSG00000079487. [A2AGH6-1]
ENSMUST00000087956; ENSMUSP00000085269; ENSMUSG00000079487. [A2AGH6-2]
GeneIDi59024.
KEGGimmu:59024.
UCSCiuc009txe.1. mouse. [A2AGH6-1]
uc009txf.1. mouse. [A2AGH6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683892 Genomic DNA. Translation: CAM24445.1 .
AL683892 Genomic DNA. Translation: CAM24446.1 .
BC057085 mRNA. Translation: AAH57085.1 .
BC057119 mRNA. Translation: AAH57119.1 .
AF071310 mRNA. Translation: AAC83164.1 . Different initiation.
AK220216 mRNA. Translation: BAD90141.1 .
CCDSi CCDS41078.1. [A2AGH6-1 ]
RefSeqi NP_067496.2. NM_021521.2. [A2AGH6-1 ]
UniGenei Mm.20873.
Mm.490933.

3D structure databases

ProteinModelPortali A2AGH6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208491. 1 interaction.
DIPi DIP-59233N.
IntActi A2AGH6. 5 interactions.
MINTi MINT-4105605.

PTM databases

PhosphoSitei A2AGH6.

Proteomic databases

MaxQBi A2AGH6.
PaxDbi A2AGH6.
PRIDEi A2AGH6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000087948 ; ENSMUSP00000085260 ; ENSMUSG00000079487 . [A2AGH6-1 ]
ENSMUST00000087956 ; ENSMUSP00000085269 ; ENSMUSG00000079487 . [A2AGH6-2 ]
GeneIDi 59024.
KEGGi mmu:59024.
UCSCi uc009txe.1. mouse. [A2AGH6-1 ]
uc009txf.1. mouse. [A2AGH6-2 ]

Organism-specific databases

CTDi 9968.
MGIi MGI:1926212. Med12.
Rougei Search...

Phylogenomic databases

eggNOGi NOG319131.
GeneTreei ENSGT00440000037505.
HOGENOMi HOG000231423.
HOVERGENi HBG052447.
InParanoidi A2AGH6.
KOi K15162.
OMAi TVDMQSN.
TreeFami TF324178.

Enzyme and pathway databases

Reactomei REACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSi Med12. mouse.
NextBioi 314592.
PROi A2AGH6.
SOURCEi Search...

Gene expression databases

Bgeei A2AGH6.
ExpressionAtlasi A2AGH6. baseline and differential.
Genevestigatori A2AGH6.

Family and domain databases

InterProi IPR019035. Mediator_Med12.
IPR021989. Mediator_Med12_catenin-bd.
IPR021990. Mediator_Med12_LCEWAV.
[Graphical view ]
Pfami PF09497. Med12. 1 hit.
PF12145. Med12-LCEWAV. 1 hit.
PF12144. Med12-PQL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-2190 (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Association of an X-chromosome dodecamer insertional variant allele with mental retardation."
    Philibert R.A., King B.H., Cook E.H., Lee Y.-H., Stubblefield B., Damschroder-Williams P., Dea C., Palotie A., Tengstrom C., Martin B.M., Ginns E.I.
    Mol. Psychiatry 3:303-309(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-2190 (ISOFORM 2).
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1045-2190 (ISOFORM 1).
    Tissue: Spleen.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-80 AND LYS-1800, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiMED12_MOUSE
AccessioniPrimary (citable) accession number: A2AGH6
Secondary accession number(s): A2AGH7
, O88542, Q571H3, Q6PGB4, Q6PGD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: November 26, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3