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A2AGH6 (MED12_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 12
Alternative name(s):
Mediator complex subunit 12
OPA-containing protein
Thyroid hormone receptor-associated protein complex 230 kDa component
Short name=Trap230
Trinucleotide repeat-containing gene 11 protein
Gene names
Name:Med12
Synonyms:Kiaa0192, Mopa, Tnrc11, Trap230
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2190 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway By similarity.

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3 By similarity.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the Mediator complex subunit 12 family.

Sequence caution

The sequence AAC83164.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   Molecular functionActivator
Repressor
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSchwann cell development

Inferred from mutant phenotype PubMed 23575864. Source: MGI

Wnt signaling pathway, planar cell polarity pathway

Inferred from mutant phenotype PubMed 20630950. Source: MGI

androgen receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

axis elongation involved in somitogenesis

Inferred from mutant phenotype PubMed 20630950. Source: MGI

canonical Wnt signaling pathway

Inferred from mutant phenotype PubMed 20630950. Source: MGI

heart development

Inferred from mutant phenotype PubMed 20630950. Source: MGI

negative regulation of Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

neural tube closure

Inferred from mutant phenotype PubMed 20630950. Source: MGI

oligodendrocyte development

Inferred from mutant phenotype PubMed 23575864. Source: MGI

stem cell maintenance

Inferred from mutant phenotype PubMed 20720539. Source: MGI

transcription initiation from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentmediator complex

Inferred from direct assay PubMed 15340084PubMed 20508642. Source: MGI

nucleoplasm

Traceable author statement. Source: Reactome

   Molecular_functionRNA polymerase II distal enhancer sequence-specific DNA binding

Inferred from direct assay PubMed 23575864. Source: MGI

RNA polymerase II transcription cofactor activity

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription

Inferred from genetic interaction PubMed 23575864. Source: MGI

chromatin binding

Inferred from direct assay PubMed 20720539. Source: MGI

protein binding

Inferred from physical interaction PubMed 23178117. Source: IntAct

receptor activity

Inferred from electronic annotation. Source: Ensembl

transcription coactivator activity

Inferred from electronic annotation. Source: Ensembl

transcription factor binding

Inferred from physical interaction PubMed 23575864. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TGFBR2P371733EBI-5744969,EBI-296151From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A2AGH6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A2AGH6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1919-1921: Missing.
     1946-1946: S → SQLSSPSL
     1966-1990: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: A2AGH6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1321: Missing.
     1919-1921: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21902190Mediator of RNA polymerase II transcription subunit 12
PRO_0000312957

Regions

Region1617 – 2063447Interaction with CTNNB1 and GLI3 By similarity

Amino acid modifications

Modified residue801N6-acetyllysine Ref.5
Modified residue1661Phosphotyrosine By similarity
Modified residue6361Phosphoserine By similarity
Modified residue12591Phosphoserine By similarity
Modified residue12701Phosphoserine By similarity
Modified residue18001N6-acetyllysine Ref.5

Natural variations

Alternative sequence1 – 13211321Missing in isoform 3.
VSP_029977
Alternative sequence1919 – 19213Missing in isoform 2 and isoform 3.
VSP_029978
Alternative sequence19461S → SQLSSPSL in isoform 2.
VSP_029979
Alternative sequence1966 – 199025Missing in isoform 2.
VSP_029980

Experimental info

Sequence conflict6741S → T in AAC83164. Ref.2
Sequence conflict15971A → T in BAD90141. Ref.4
Sequence conflict21711L → I in AAC83164. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 146E05DCCFF38DB4

FASTA2,190244,561
        10         20         30         40         50         60 
MAAFGILSYE HRPLKRLRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA VSGDEHGSAK 

        70         80         90        100        110        120 
NVNFNPAKIS SNFSSIIAEK LRCNTLSDTG RRKSLMNQKD NFWLVTARSQ SAINTWFTDL 

       130        140        150        160        170        180 
AGTKPLTHLA KKVPIFSKKE EVFGYLAKYT VPVMRAAWLI KMTCAYYAAM SETKVKKKNT 

       190        200        210        220        230        240 
ADPFTEWTQI ITKYLWEQLQ KMAEYYRPGP AGSGGCGSTI GPLPHDVEMA IRQWDYNEKL 

       250        260        270        280        290        300 
ALFMFQDGML DRHEFLTWVL ECFEKIRPGE DELLKLLLPL LLRYSGEFVQ SAYLSRRLAY 

       310        320        330        340        350        360 
FCTRRLALQL DGVSSHSSHV IAAQSTSSLP TTPAPQPPTS STPSTPFSDL LMCPQHRPLV 

       370        380        390        400        410        420 
FGLSCILQTI LLCCPSALVW HYSLTDSRIK TGSPLDHLPI APSNLPMPEG NSAFTQQVRA 

       430        440        450        460        470        480 
KLREIEQQIK ERGQAVEVRW SFDKCQEATA GFTIGRVLHT LEVLDSHSFE RSDFSNSLDS 

       490        500        510        520        530        540 
LCNRIFGLGP SKDGHEISSD DDAVVSLLCE WAVSCKRSGR HRAMVVAKLL EKRQAEIEAE 

       550        560        570        580        590        600 
RCGESEAADE KGSVASGSLS APSAPIFQDV LLQFLDTQAP MLTDPRSESE RVEFFNLVLL 

       610        620        630        640        650        660 
FCELIRHDVF SHNMYTCTLI SRGDLAFGAP GPRPPSPFDD PTDDPERKEA EGSSSSKLED 

       670        680        690        700        710        720 
PGLSESMDID PSSSVLFEDM EKPDFSLFSP TMPCEGKGSP SPEKPDVEKE VKPPAKEKIE 

       730        740        750        760        770        780 
GTLGILYDQP RHVQYATHFP IPQEESCSHE CNQRLVVLFG VGKQRDDARH AIKKITKDIL 

       790        800        810        820        830        840 
KVLNRKGTAE TDQLAPIVPL NPGDLTFLGG EDGQKRRRNR PEAFPTAEDI FAKFQHLSHY 

       850        860        870        880        890        900 
DQHQVTAQVS RNVLEQITSF ALGMSYHLPL VQHVQFIFDL MEYSLSISGL IDFAIQLLNE 

       910        920        930        940        950        960 
LSVVEAELLL KSSDLVGSYT TSLCLCIVAV LRHYHACLIL NQDQMAQVFE GLCGVVKHGM 

       970        980        990       1000       1010       1020 
NRSDGSSAER CILAYLYDLY TSCSHLKSKF GELFSDFCSK VKNTIYCNVE PSESNMRWAP 

      1030       1040       1050       1060       1070       1080 
EFMIDTLENP AAHTFTYTGL GKSLSENPAN RYSFVCNALM HVCVGHHDPD RVNDIAILCA 

      1090       1100       1110       1120       1130       1140 
ELTGYCKSLS AEWLGVLKAL CCSSNNGTCG FNDLLCNVDV SDLSFHDSLA TFVAILIARQ 

      1150       1160       1170       1180       1190       1200 
CLLLEDLIRC AAIPSLLNAA CSEQDSEPGA RLTCRILLHL FKTPQLNPCQ SDGNKPTVGI 

      1210       1220       1230       1240       1250       1260 
RSSCDRHLLA ASQNRIVDGA VFAVLKAVFV LGDAELKGSG FTVPGGTEEL PEEEGGGGSS 

      1270       1280       1290       1300       1310       1320 
GRRQGGRNIS VETASLDVYA KYVLRSICQQ EWVGERCLKS LCEDSNDLQD PVLSSAQAQR 

      1330       1340       1350       1360       1370       1380 
LMQLICYPHR LLDNEDGENP QRQRIKRILK NLDQWTMRQS SLELQLMIKQ TPNTEMNSLL 

      1390       1400       1410       1420       1430       1440 
ENIAKATIEV FQQSAETGSS SGSTASNMPS SSKTKPVLSS LERSGVWLVA PLIAKLPTSV 

      1450       1460       1470       1480       1490       1500 
QGHVLKAAGE ELEKGQHLGS SSRKERDRQK QKSMSLLSQQ PFLSLVLTCL KGQDEQREGL 

      1510       1520       1530       1540       1550       1560 
LASLHSQVHQ IVINWRENQY LDDCKPKQLM HEALKLRLNL VGGMFDTVQR STQQTTEWAQ 

      1570       1580       1590       1600       1610       1620 
LLLEIIISGT VDMQSNNELF TTVLDMLSVL INGTLAADMS SISQGSMEEN KRAYMNLVKK 

      1630       1640       1650       1660       1670       1680 
LQKDLGERQS DSLEKVHQLL PLPKQNRDVI TCEPQGSLID TKGNKIAGFD SIFKKEGLQV 

      1690       1700       1710       1720       1730       1740 
STKQKISPWE LFEGLKPSTA PLSWAWFGTV RVDRRVARGE EQQRLLLYHT HLRPRPRAYY 

      1750       1760       1770       1780       1790       1800 
LEPLPLPPED EEPPAPALLE PEKKAPEPPK TDKPGAAPPS TEERKKKSTK GKKRSQPATK 

      1810       1820       1830       1840       1850       1860 
NEDYGMGPGR SGPYGVTVPP DLLHHANPGS ISHLSYRQSS MGLYTQNQPL PAGGPRVDPY 

      1870       1880       1890       1900       1910       1920 
RPVRLPMQKL PTRPTYPGVL PTTMSTVMGL EPSSYKTSVY RQQQPTVPQG QRLRQQLQAK 

      1930       1940       1950       1960       1970       1980 
IQSQGMLGQS SVHQMTPSSS YGLQTSQGYT SYVSHVGLQQ HTGPAGTMVP PSYSSQPYQS 

      1990       2000       2010       2020       2030       2040 
THPSTNPTLV DPTRHLQQRP SGYVHQQAPT YGHGLTSTQR FSHQTLQQTP MMGTMTPLSA 

      2050       2060       2070       2080       2090       2100 
QGVQAGVRST SILPEQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQYH IRQQQQQQQM 

      2110       2120       2130       2140       2150       2160 
LRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQP HQQQQQAAPP QPQPQSQPQF QRQGLQQTQQ 

      2170       2180       2190 
QQQTAALVRQ LQQQLSNTQP QPSTNIFGRY 

« Hide

Isoform 2 [UniParc].

Checksum: 8BE79B8DEFD56844
Show »

FASTA2,169242,303
Isoform 3 [UniParc].

Checksum: 4A782193143882B0
Show »

FASTA86697,885

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-2190 (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[3]"Association of an X-chromosome dodecamer insertional variant allele with mental retardation."
Philibert R.A., King B.H., Cook E.H., Lee Y.-H., Stubblefield B., Damschroder-Williams P., Dea C., Palotie A., Tengstrom C., Martin B.M., Ginns E.I.
Mol. Psychiatry 3:303-309(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-2190 (ISOFORM 2).
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1045-2190 (ISOFORM 1).
Tissue: Spleen.
[5]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-80 AND LYS-1800, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL683892 Genomic DNA. Translation: CAM24445.1.
AL683892 Genomic DNA. Translation: CAM24446.1.
BC057085 mRNA. Translation: AAH57085.1.
BC057119 mRNA. Translation: AAH57119.1.
AF071310 mRNA. Translation: AAC83164.1. Different initiation.
AK220216 mRNA. Translation: BAD90141.1.
CCDSCCDS41078.1. [A2AGH6-1]
RefSeqNP_067496.2. NM_021521.2. [A2AGH6-1]
UniGeneMm.20873.
Mm.490933.

3D structure databases

ProteinModelPortalA2AGH6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208491. 1 interaction.
DIPDIP-59233N.
IntActA2AGH6. 5 interactions.
MINTMINT-4105605.

PTM databases

PhosphoSiteA2AGH6.

Proteomic databases

MaxQBA2AGH6.
PaxDbA2AGH6.
PRIDEA2AGH6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000087948; ENSMUSP00000085260; ENSMUSG00000079487. [A2AGH6-1]
ENSMUST00000087956; ENSMUSP00000085269; ENSMUSG00000079487. [A2AGH6-2]
GeneID59024.
KEGGmmu:59024.
UCSCuc009txe.1. mouse. [A2AGH6-1]
uc009txf.1. mouse. [A2AGH6-2]

Organism-specific databases

CTD9968.
MGIMGI:1926212. Med12.
RougeSearch...

Phylogenomic databases

eggNOGNOG319131.
GeneTreeENSGT00440000037505.
HOGENOMHOG000231423.
HOVERGENHBG052447.
InParanoidA2AGH6.
KOK15162.
OMATVDMQSN.
TreeFamTF324178.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_200794. Mus musculus biological processes.

Gene expression databases

BgeeA2AGH6.
GenevestigatorA2AGH6.

Family and domain databases

InterProIPR019035. Mediator_Med12.
IPR021989. Mediator_Med12_catenin-bd.
IPR021990. Mediator_Med12_LCEWAV.
[Graphical view]
PfamPF09497. Med12. 1 hit.
PF12145. Med12-LCEWAV. 1 hit.
PF12144. Med12-PQL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio314592.
PROA2AGH6.
SOURCESearch...

Entry information

Entry nameMED12_MOUSE
AccessionPrimary (citable) accession number: A2AGH6
Secondary accession number(s): A2AGH7 expand/collapse secondary AC list , O88542, Q571H3, Q6PGB4, Q6PGD8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot