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A2AGH6

- MED12_MOUSE

UniProt

A2AGH6 - MED12_MOUSE

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Protein

Mediator of RNA polymerase II transcription subunit 12

Gene
Med12, Kiaa0192, Mopa, Tnrc11, Trap230
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway By similarity.

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. protein binding Source: IntAct
  3. receptor activity Source: Ensembl
  4. RNA polymerase II distal enhancer sequence-specific DNA binding Source: MGI
  5. RNA polymerase II transcription cofactor activity Source: Ensembl
  6. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI
  7. transcription coactivator activity Source: Ensembl
  8. transcription factor binding Source: MGI

GO - Biological processi

  1. androgen receptor signaling pathway Source: Ensembl
  2. axis elongation involved in somitogenesis Source: MGI
  3. canonical Wnt signaling pathway Source: MGI
  4. heart development Source: MGI
  5. negative regulation of Wnt signaling pathway Source: Ensembl
  6. neural tube closure Source: MGI
  7. oligodendrocyte development Source: MGI
  8. Schwann cell development Source: MGI
  9. stem cell maintenance Source: MGI
  10. transcription initiation from RNA polymerase II promoter Source: Ensembl
  11. Wnt signaling pathway, planar cell polarity pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 12
Alternative name(s):
Mediator complex subunit 12
OPA-containing protein
Thyroid hormone receptor-associated protein complex 230 kDa component
Short name:
Trap230
Trinucleotide repeat-containing gene 11 protein
Gene namesi
Name:Med12
Synonyms:Kiaa0192, Mopa, Tnrc11, Trap230
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1926212. Med12.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21902190Mediator of RNA polymerase II transcription subunit 12PRO_0000312957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801N6-acetyllysine1 Publication
Modified residuei166 – 1661Phosphotyrosine By similarity
Modified residuei636 – 6361Phosphoserine By similarity
Modified residuei1259 – 12591Phosphoserine By similarity
Modified residuei1270 – 12701Phosphoserine By similarity
Modified residuei1800 – 18001N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiA2AGH6.
PaxDbiA2AGH6.
PRIDEiA2AGH6.

PTM databases

PhosphoSiteiA2AGH6.

Expressioni

Gene expression databases

BgeeiA2AGH6.
GenevestigatoriA2AGH6.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
TGFBR2P371733EBI-5744969,EBI-296151From a different organism.

Protein-protein interaction databases

BioGridi208491. 1 interaction.
DIPiDIP-59233N.
IntActiA2AGH6. 5 interactions.
MINTiMINT-4105605.

Structurei

3D structure databases

ProteinModelPortaliA2AGH6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1617 – 2063447Interaction with CTNNB1 and GLI3 By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG319131.
GeneTreeiENSGT00440000037505.
HOGENOMiHOG000231423.
HOVERGENiHBG052447.
InParanoidiA2AGH6.
KOiK15162.
OMAiTVDMQSN.
TreeFamiTF324178.

Family and domain databases

InterProiIPR019035. Mediator_Med12.
IPR021989. Mediator_Med12_catenin-bd.
IPR021990. Mediator_Med12_LCEWAV.
[Graphical view]
PfamiPF09497. Med12. 1 hit.
PF12145. Med12-LCEWAV. 1 hit.
PF12144. Med12-PQL. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A2AGH6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAFGILSYE HRPLKRLRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA     50
VSGDEHGSAK NVNFNPAKIS SNFSSIIAEK LRCNTLSDTG RRKSLMNQKD 100
NFWLVTARSQ SAINTWFTDL AGTKPLTHLA KKVPIFSKKE EVFGYLAKYT 150
VPVMRAAWLI KMTCAYYAAM SETKVKKKNT ADPFTEWTQI ITKYLWEQLQ 200
KMAEYYRPGP AGSGGCGSTI GPLPHDVEMA IRQWDYNEKL ALFMFQDGML 250
DRHEFLTWVL ECFEKIRPGE DELLKLLLPL LLRYSGEFVQ SAYLSRRLAY 300
FCTRRLALQL DGVSSHSSHV IAAQSTSSLP TTPAPQPPTS STPSTPFSDL 350
LMCPQHRPLV FGLSCILQTI LLCCPSALVW HYSLTDSRIK TGSPLDHLPI 400
APSNLPMPEG NSAFTQQVRA KLREIEQQIK ERGQAVEVRW SFDKCQEATA 450
GFTIGRVLHT LEVLDSHSFE RSDFSNSLDS LCNRIFGLGP SKDGHEISSD 500
DDAVVSLLCE WAVSCKRSGR HRAMVVAKLL EKRQAEIEAE RCGESEAADE 550
KGSVASGSLS APSAPIFQDV LLQFLDTQAP MLTDPRSESE RVEFFNLVLL 600
FCELIRHDVF SHNMYTCTLI SRGDLAFGAP GPRPPSPFDD PTDDPERKEA 650
EGSSSSKLED PGLSESMDID PSSSVLFEDM EKPDFSLFSP TMPCEGKGSP 700
SPEKPDVEKE VKPPAKEKIE GTLGILYDQP RHVQYATHFP IPQEESCSHE 750
CNQRLVVLFG VGKQRDDARH AIKKITKDIL KVLNRKGTAE TDQLAPIVPL 800
NPGDLTFLGG EDGQKRRRNR PEAFPTAEDI FAKFQHLSHY DQHQVTAQVS 850
RNVLEQITSF ALGMSYHLPL VQHVQFIFDL MEYSLSISGL IDFAIQLLNE 900
LSVVEAELLL KSSDLVGSYT TSLCLCIVAV LRHYHACLIL NQDQMAQVFE 950
GLCGVVKHGM NRSDGSSAER CILAYLYDLY TSCSHLKSKF GELFSDFCSK 1000
VKNTIYCNVE PSESNMRWAP EFMIDTLENP AAHTFTYTGL GKSLSENPAN 1050
RYSFVCNALM HVCVGHHDPD RVNDIAILCA ELTGYCKSLS AEWLGVLKAL 1100
CCSSNNGTCG FNDLLCNVDV SDLSFHDSLA TFVAILIARQ CLLLEDLIRC 1150
AAIPSLLNAA CSEQDSEPGA RLTCRILLHL FKTPQLNPCQ SDGNKPTVGI 1200
RSSCDRHLLA ASQNRIVDGA VFAVLKAVFV LGDAELKGSG FTVPGGTEEL 1250
PEEEGGGGSS GRRQGGRNIS VETASLDVYA KYVLRSICQQ EWVGERCLKS 1300
LCEDSNDLQD PVLSSAQAQR LMQLICYPHR LLDNEDGENP QRQRIKRILK 1350
NLDQWTMRQS SLELQLMIKQ TPNTEMNSLL ENIAKATIEV FQQSAETGSS 1400
SGSTASNMPS SSKTKPVLSS LERSGVWLVA PLIAKLPTSV QGHVLKAAGE 1450
ELEKGQHLGS SSRKERDRQK QKSMSLLSQQ PFLSLVLTCL KGQDEQREGL 1500
LASLHSQVHQ IVINWRENQY LDDCKPKQLM HEALKLRLNL VGGMFDTVQR 1550
STQQTTEWAQ LLLEIIISGT VDMQSNNELF TTVLDMLSVL INGTLAADMS 1600
SISQGSMEEN KRAYMNLVKK LQKDLGERQS DSLEKVHQLL PLPKQNRDVI 1650
TCEPQGSLID TKGNKIAGFD SIFKKEGLQV STKQKISPWE LFEGLKPSTA 1700
PLSWAWFGTV RVDRRVARGE EQQRLLLYHT HLRPRPRAYY LEPLPLPPED 1750
EEPPAPALLE PEKKAPEPPK TDKPGAAPPS TEERKKKSTK GKKRSQPATK 1800
NEDYGMGPGR SGPYGVTVPP DLLHHANPGS ISHLSYRQSS MGLYTQNQPL 1850
PAGGPRVDPY RPVRLPMQKL PTRPTYPGVL PTTMSTVMGL EPSSYKTSVY 1900
RQQQPTVPQG QRLRQQLQAK IQSQGMLGQS SVHQMTPSSS YGLQTSQGYT 1950
SYVSHVGLQQ HTGPAGTMVP PSYSSQPYQS THPSTNPTLV DPTRHLQQRP 2000
SGYVHQQAPT YGHGLTSTQR FSHQTLQQTP MMGTMTPLSA QGVQAGVRST 2050
SILPEQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQYH IRQQQQQQQM 2100
LRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQP HQQQQQAAPP QPQPQSQPQF 2150
QRQGLQQTQQ QQQTAALVRQ LQQQLSNTQP QPSTNIFGRY 2190
Length:2,190
Mass (Da):244,561
Last modified:February 20, 2007 - v1
Checksum:i146E05DCCFF38DB4
GO
Isoform 2 (identifier: A2AGH6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1919-1921: Missing.
     1946-1946: S → SQLSSPSL
     1966-1990: Missing.

Note: No experimental confirmation available.

Show »
Length:2,169
Mass (Da):242,303
Checksum:i8BE79B8DEFD56844
GO
Isoform 3 (identifier: A2AGH6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1321: Missing.
     1919-1921: Missing.

Note: No experimental confirmation available.

Show »
Length:866
Mass (Da):97,885
Checksum:i4A782193143882B0
GO

Sequence cautioni

The sequence AAC83164.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 13211321Missing in isoform 3. VSP_029977Add
BLAST
Alternative sequencei1919 – 19213Missing in isoform 2 and isoform 3. VSP_029978
Alternative sequencei1946 – 19461S → SQLSSPSL in isoform 2. VSP_029979
Alternative sequencei1966 – 199025Missing in isoform 2. VSP_029980Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti674 – 6741S → T in AAC83164. 1 Publication
Sequence conflicti1597 – 15971A → T in BAD90141. 1 Publication
Sequence conflicti2171 – 21711L → I in AAC83164. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL683892 Genomic DNA. Translation: CAM24445.1.
AL683892 Genomic DNA. Translation: CAM24446.1.
BC057085 mRNA. Translation: AAH57085.1.
BC057119 mRNA. Translation: AAH57119.1.
AF071310 mRNA. Translation: AAC83164.1. Different initiation.
AK220216 mRNA. Translation: BAD90141.1.
CCDSiCCDS41078.1. [A2AGH6-1]
RefSeqiNP_067496.2. NM_021521.2. [A2AGH6-1]
UniGeneiMm.20873.
Mm.490933.

Genome annotation databases

EnsembliENSMUST00000087948; ENSMUSP00000085260; ENSMUSG00000079487. [A2AGH6-1]
ENSMUST00000087956; ENSMUSP00000085269; ENSMUSG00000079487. [A2AGH6-2]
GeneIDi59024.
KEGGimmu:59024.
UCSCiuc009txe.1. mouse. [A2AGH6-1]
uc009txf.1. mouse. [A2AGH6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL683892 Genomic DNA. Translation: CAM24445.1 .
AL683892 Genomic DNA. Translation: CAM24446.1 .
BC057085 mRNA. Translation: AAH57085.1 .
BC057119 mRNA. Translation: AAH57119.1 .
AF071310 mRNA. Translation: AAC83164.1 . Different initiation.
AK220216 mRNA. Translation: BAD90141.1 .
CCDSi CCDS41078.1. [A2AGH6-1 ]
RefSeqi NP_067496.2. NM_021521.2. [A2AGH6-1 ]
UniGenei Mm.20873.
Mm.490933.

3D structure databases

ProteinModelPortali A2AGH6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208491. 1 interaction.
DIPi DIP-59233N.
IntActi A2AGH6. 5 interactions.
MINTi MINT-4105605.

PTM databases

PhosphoSitei A2AGH6.

Proteomic databases

MaxQBi A2AGH6.
PaxDbi A2AGH6.
PRIDEi A2AGH6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000087948 ; ENSMUSP00000085260 ; ENSMUSG00000079487 . [A2AGH6-1 ]
ENSMUST00000087956 ; ENSMUSP00000085269 ; ENSMUSG00000079487 . [A2AGH6-2 ]
GeneIDi 59024.
KEGGi mmu:59024.
UCSCi uc009txe.1. mouse. [A2AGH6-1 ]
uc009txf.1. mouse. [A2AGH6-2 ]

Organism-specific databases

CTDi 9968.
MGIi MGI:1926212. Med12.
Rougei Search...

Phylogenomic databases

eggNOGi NOG319131.
GeneTreei ENSGT00440000037505.
HOGENOMi HOG000231423.
HOVERGENi HBG052447.
InParanoidi A2AGH6.
KOi K15162.
OMAi TVDMQSN.
TreeFami TF324178.

Enzyme and pathway databases

Reactomei REACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

NextBioi 314592.
PROi A2AGH6.
SOURCEi Search...

Gene expression databases

Bgeei A2AGH6.
Genevestigatori A2AGH6.

Family and domain databases

InterProi IPR019035. Mediator_Med12.
IPR021989. Mediator_Med12_catenin-bd.
IPR021990. Mediator_Med12_LCEWAV.
[Graphical view ]
Pfami PF09497. Med12. 1 hit.
PF12145. Med12-LCEWAV. 1 hit.
PF12144. Med12-PQL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-2190 (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Association of an X-chromosome dodecamer insertional variant allele with mental retardation."
    Philibert R.A., King B.H., Cook E.H., Lee Y.-H., Stubblefield B., Damschroder-Williams P., Dea C., Palotie A., Tengstrom C., Martin B.M., Ginns E.I.
    Mol. Psychiatry 3:303-309(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-2190 (ISOFORM 2).
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1045-2190 (ISOFORM 1).
    Tissue: Spleen.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-80 AND LYS-1800, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiMED12_MOUSE
AccessioniPrimary (citable) accession number: A2AGH6
Secondary accession number(s): A2AGH7
, O88542, Q571H3, Q6PGB4, Q6PGD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi