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A2AF47 (DOC11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dedicator of cytokinesis protein 11
Alternative name(s):
Activated Cdc42-associated guanine nucleotide exchange factor
Short name=ACG
Zizimin-2
Gene names
Name:Dock11
Synonyms:Ziz2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2073 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Ref.1 Ref.3

Subunit structure

Interacts with CDC42. Ref.1 Ref.3

Tissue specificity

Expressed in spleen, thymus, bone marrow and peripheral blood lymphocytes. Enriched in B-cells from germinal centers. Ref.1

Domain

The DHR-2 domain is necessary for the GEF activity.

Miscellaneous

'Zizim' means 'spike' in Hebrew.

Sequence similarities

Belongs to the DOCK family.

Contains 1 DHR-1 domain.

Contains 1 DHR-2 domain.

Contains 1 PH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20732073Dedicator of cytokinesis protein 11
PRO_0000299559

Regions

Domain165 – 272108PH
Domain640 – 818179DHR-1
Domain1609 – 2036428DHR-2
Region66 – 12661Interaction with activated CDC42

Amino acid modifications

Modified residue1611Phosphoserine By similarity
Modified residue2481Phosphotyrosine By similarity
Modified residue2511Phosphotyrosine By similarity

Experimental info

Sequence conflict16081A → T in BAD83670. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A2AF47 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 2907066969A6A8F8

FASTA2,073237,771
        10         20         30         40         50         60 
MAEVRKFTKR LSKPGTAAEL RQSVSEAVRG SVVLEKAKLV EPLDYENVIT QRKTQIYSDP 

        70         80         90        100        110        120 
LRDLLMFPME DISISVIGRQ RRTVQSTVPE DAEKRAQSLF VKECIKTYST DWHVVNYKYE 

       130        140        150        160        170        180 
DFSGDFRMLP CKSLRPEKIP NHVFEIDEDC EKDEDSSSLC SQKGGVIKQG WLHKANVNST 

       190        200        210        220        230        240 
ITVTMKVFKR RYFYLTQLPD GSYILNSYKD EKNSKESKGC IYLDACIDVV QCPKMRRHAF 

       250        260        270        280        290        300 
ELKMLDKYSH YLAAETEQEM EEWLIMLKKI IQINTDSLVQ EKKDTVEAIQ EEETSSQGKA 

       310        320        330        340        350        360 
ENIMASLERS MHPELMKYGR ETEQLNKLSR GDGRQNLFSF DSEVQRLDFS GIEPDVKPFE 

       370        380        390        400        410        420 
EKCNKRFMVN CHDLTFNILG HIGDNAKGPP TNVEPFFINL ALFDVKNNCK ISADFHVDLN 

       430        440        450        460        470        480 
PPSVREMLWG TSTQLSNDGN AKGFSPESLI HGIAESQLCY IKQGIFSVTN PHPEIFLVVR 

       490        500        510        520        530        540 
IEKVLQGNIT HCAEPYIKNS DPIKTAQKVH RTAKQVCSRL GQYRMPFAWA ARPIFKDVQG 

       550        560        570        580        590        600 
SLDLDGRFSP LYKQDSSKLS NEDILKLLSE YKKPEKTKLQ IIPGQLSITV ECVPVDLPNC 

       610        620        630        640        650        660 
ITSSYVPLKP FEKNCQNITV EVEEFVPEMT KYCYPFTIYK NHLYVYPLQL KYDSQKSFAK 

       670        680        690        700        710        720 
ARNIAVCVEF RDSDESDASA LKCIYGKPAG SVFTTNAYAV VSHHNQNPEF YDEIKIELPI 

       730        740        750        760        770        780 
HLHQKHHLLF TFYHVSCEIN TKGTTKKQDT VETPVGFAWV PLLKDGRVIT LEQQLPVSAN 

       790        800        810        820        830        840 
LPPGYLNVND AESRRQSNAD IKWVDGAKPL LKIKTHLEST IYTQDLHVHK FFHHCQLIQS 

       850        860        870        880        890        900 
GSKEVPGELI KYLKCLHAME IQVMIQFLPV ILMQLFRVLT NMTHEDDVPI NCTMVLLHIV 

       910        920        930        940        950        960 
SKCHEEGLES YLRSFIKYSF RPEKPSTLQA QLIHETLATT MIAILKQSAD FLAINKLLKY 

       970        980        990       1000       1010       1020 
SWFFFEIIAK SMATYLLEEN KIKLPRGQRF PEAYHHVLHS LLLAIIPHVT IRYAEIPDES 

      1030       1040       1050       1060       1070       1080 
RNGNYSLASF LKRCLTLMDR GFVFNLINDY ISGFSPKDPK VLAEYKFEFL QTICNHEHYI 

      1090       1100       1110       1120       1130       1140 
PLNLPMAFAK PKLQRVQDSN LEYSLSDEYC KHHFLVGLLL RETSIALQDN YEIRYTAISV 

      1150       1160       1170       1180       1190       1200 
IKNLLIKHAF DTRYQHKNQQ AKIAQLYLPF VGLLLENIQR LAGRDTLYSC AAMPSSASRD 

      1210       1220       1230       1240       1250       1260 
EFPCGFVSPT NRGSLASDKD TAYGSFQNGH GIKREDSRGS LIPEGATGFP DPGSTSENTR 

      1270       1280       1290       1300       1310       1320 
QSSSRSSVSQ YNRLDQYEIR NLLMCYLYIV KMISEDTLLT YWNKVSPQEL INILVLLEVC 

      1330       1340       1350       1360       1370       1380 
LFHFRYMGKR NIARVHDAWL SKHFGIDRKS QTMPALRNRS GVMQARLQHL SSLESSFTLN 

      1390       1400       1410       1420       1430       1440 
HSSATTEADI FHQALLEGNT ATEVSLTVLD TISFFTQCFK NQLLNNDGHN PLMKKVFDIH 

      1450       1460       1470       1480       1490       1500 
LAFLKNGQSE VSLKHVFASL RSFISKFPSA FFKGRVNMCA AFCYEVLKCC TSKISSTRNE 

      1510       1520       1530       1540       1550       1560 
ASALLYLLMR NNFEYTKRKT FLRTHLQIII AVSQLIADVA LSGGSRFQES LFIINNFANS 

      1570       1580       1590       1600       1610       1620 
DRPMKATAFP TEVKDLTKRI RTVLMATAQM KEHEKDPEML IDLQYSLAKS YASTPELRKT 

      1630       1640       1650       1660       1670       1680 
WLDSMAKIHI KNGDFSEAAM CYVHVAALVA EFLHRKKLFP SGCSAFKKIT PNIDEEGAMK 

      1690       1700       1710       1720       1730       1740 
EDAGMMDVHY SEEVLLELLE QCVDGLWKAE RYEVISEISK LIIPIYEKRR EFEKLTQVYR 

      1750       1760       1770       1780       1790       1800 
TLHGAYTKIL EVMHTKKRLL GTFFRVAFYG QSFFEEEDGK EYIYKEPKLT GLSEISLRLV 

      1810       1820       1830       1840       1850       1860 
KLYGEKFGTE NVKIIQDSDK VNAKELDPKF AHIQVTYVKP YFDDKELTER KTEFERNHNI 

      1870       1880       1890       1900       1910       1920 
NRFVFEAPYT LSGKKQGCIE EQCKRRTILT TSNSFPYVKK RIPINCEQQV NLKPIDVATD 

      1930       1940       1950       1960       1970       1980 
EIKDKTAELH KLCSSVDVDM IQLQLKLQGC VSVQVNAGPL AYARAFLNES QANKYPPKKV 

      1990       2000       2010       2020       2030       2040 
NELKDMFRKF IQACSIALEL NERLIKEDQI EYHEGLKSNF RDMVKELSDI IHEQILQEDT 

      2050       2060       2070 
MHSPWMNNTL HVFCAISGTS SNRGYGSPRY AEV 

« Hide

References

« Hide 'large scale' references
[1]"Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes."
Nishikimi A., Meller N., Uekawa N., Isobe K., Schwartz M.A., Maruyama M.
FEBS Lett. 579:1039-1046(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDC42, TISSUE SPECIFICITY, FUNCTION.
Tissue: Spleen.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Identification of a DOCK180-related guanine nucleotide exchange factor that is capable of mediating a positive feedback activation of Cdc42."
Lin Q., Yang W., Baird D., Feng Q., Cerione R.A.
J. Biol. Chem. 281:35253-35262(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CDC42, FUNCTION.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB116935 mRNA. Translation: BAD83670.1.
AL672038, AL672023 Genomic DNA. Translation: CAM20638.1.
AL672023, AL672038 Genomic DNA. Translation: CAM21480.1.
CCDSCCDS40923.1.
RefSeqNP_001009947.2. NM_001009947.3.
UniGeneMm.32873.

3D structure databases

ProteinModelPortalA2AF47.
SMRA2AF47. Positions 162-298, 1613-2035.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid217880. 1 interaction.

PTM databases

PhosphoSiteA2AF47.

Proteomic databases

MaxQBA2AF47.
PaxDbA2AF47.
PRIDEA2AF47.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033419; ENSMUSP00000033419; ENSMUSG00000031093.
GeneID75974.
KEGGmmu:75974.
UCSCuc009sxi.1. mouse.

Organism-specific databases

CTD139818.
MGIMGI:1923224. Dock11.

Phylogenomic databases

eggNOGNOG242127.
GeneTreeENSGT00750000117541.
HOGENOMHOG000231373.
HOVERGENHBG107819.
InParanoidA2AF47.
OMAMAKIHIK.
OrthoDBEOG76DTRQ.
TreeFamTF313629.

Gene expression databases

ArrayExpressA2AF47.
BgeeA2AF47.
CleanExMM_DOCK11.
GenevestigatorA2AF47.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PANTHERPTHR23317. PTHR23317. 1 hit.
PfamPF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 4 hits.
PROSITEPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio344361.
PROA2AF47.
SOURCESearch...

Entry information

Entry nameDOC11_MOUSE
AccessionPrimary (citable) accession number: A2AF47
Secondary accession number(s): Q5KTP7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot