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Protein

Dynein intermediate chain 2, axonemal

Gene

Dnai2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the dynein complex of respiratory cilia.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein intermediate chain 2, axonemal
Alternative name(s):
Axonemal dynein intermediate chain 2
Gene namesi
Name:Dnai2
Synonyms:Dnaic2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2685574. Dnaic2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Dynein intermediate chain 2, axonemalPRO_0000365816Add
BLAST

Proteomic databases

MaxQBiA2AC93.
PaxDbiA2AC93.
PRIDEiA2AC93.

PTM databases

iPTMnetiA2AC93.
PhosphoSiteiA2AC93.

Expressioni

Tissue specificityi

Predominantly expressed in ovary, testis and lung.1 Publication

Developmental stagei

In ovaries, it is detected at high levels in vivo on day 10, with a subsequent decrease on days 15 and 20, in adult and old ovaries. Weakly expressed on day 5.1 Publication

Gene expression databases

BgeeiA2AC93.
ExpressionAtlasiA2AC93. baseline and differential.
GenevisibleiA2AC93. MM.

Interactioni

Subunit structurei

Consists of at least two heavy chains and a number of intermediate and light chains (Probable). Interacts with DNAAF2 (PubMed:19052621). Interacts with PIH1D3 (PubMed:24421334). Interacts with HEATR2; probably involved in outer arm dynein assembly (By similarity).By similarityCurated2 Publications

Protein-protein interaction databases

DIPiDIP-59778N.
STRINGi10090.ENSMUSP00000065787.

Structurei

3D structure databases

ProteinModelPortaliA2AC93.
SMRiA2AC93. Positions 228-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati214 – 25441WD 1Add
BLAST
Repeati261 – 30242WD 2Add
BLAST
Repeati362 – 40140WD 3Add
BLAST
Repeati405 – 44541WD 4Add
BLAST
Repeati450 – 48940WD 5Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi501 – 619119Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated
Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IU37. Eukaryota.
ENOG410XTD6. LUCA.
GeneTreeiENSGT00840000129862.
HOGENOMiHOG000259651.
HOVERGENiHBG013035.
InParanoidiA2AC93.
KOiK11143.
OMAiETRGINH.
OrthoDBiEOG70GMF5.
PhylomeDBiA2AC93.
TreeFamiTF300553.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2AC93-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEIVYVYLKK RSEFGKQCNF SDRQAELNID ILPNPELAAL YVERNPVDTG
60 70 80 90 100
IQCSASMSEH EANTERFEME SCGVNHVEGG WPKDVNPQEL EQTIRFRKKV
110 120 130 140 150
EKDENYINAV MQLGSIMEHC IKQNNAIDIY EEYFDDEEAV EVTEEAPSAK
160 170 180 190 200
TINVFRDPQE IKRTATHLSW HPDGNRKLAV AYSCLKFQRA PMSMNYDSYI
210 220 230 240 250
WDLENPNRPE IALKPLSPLV TLEYNPKDSH VLLGGCYNGQ IACWDTRKGS
260 270 280 290 300
LVAELSTIEF SHRDPVYGTI WLQSKTGTEC FSASTDGQVM WWDIRKISEP
310 320 330 340 350
IEVVIMDISR KEQLENALGA ISLEFESTLP TKFMVGTEQG IVISCNRKAK
360 370 380 390 400
TQAEKIVCTF YGHHGPIYAL QRNPFYPKNF LTVGDWTARI WSEDSRESSI
410 420 430 440 450
MWTKYHMAYL SDGAWSPVRP AVFFTTKMDG TLDIWDLVFK QCDPALSLKV
460 470 480 490 500
CDDPLFCLRV QDNGCLIACG SELGTTTLLE VSSSLSTLQR NEKNIASSIF
510 520 530 540 550
ERETRREKIL EARHREMRLK EKGKVEGKED DQKEEEAALD LDELVGKAEE
560 570 580 590 600
EFFEVIFSEL KRKEAEALKK KPKPRKKSSV KVEAEEEVEE NVGEEEEAGG
610 620
IIGIDAVEDM SEEAGEEQED VPT
Length:623
Mass (Da):70,968
Last modified:March 3, 2009 - v2
Checksum:iA31B5EF749EF7FF4
GO
Isoform 2 (identifier: A2AC93-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     450-461: Missing.

Show »
Length:611
Mass (Da):69,578
Checksum:iA852565A51265DDA
GO

Sequence cautioni

The sequence CAM19252.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei450 – 46112Missing in isoform 2. CuratedVSP_036542Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF565270 mRNA. Translation: ABU62629.1.
AL663079 Genomic DNA. Translation: CAM19252.1. Sequence problems.
AL663079 Genomic DNA. Translation: CAM19253.1.
CH466558 Genomic DNA. Translation: EDL34441.1.
CCDSiCCDS36366.1. [A2AC93-1]
RefSeqiNP_001030050.2. NM_001034878.2. [A2AC93-1]
XP_006533723.1. XM_006533660.2. [A2AC93-1]
XP_006533724.1. XM_006533661.1. [A2AC93-1]
XP_006533725.1. XM_006533662.2. [A2AC93-1]
UniGeneiMm.212764.

Genome annotation databases

EnsembliENSMUST00000069325; ENSMUSP00000065787; ENSMUSG00000034706. [A2AC93-1]
ENSMUST00000092469; ENSMUSP00000090126; ENSMUSG00000034706. [A2AC93-1]
GeneIDi432611.
KEGGimmu:432611.
UCSCiuc007mfn.1. mouse. [A2AC93-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF565270 mRNA. Translation: ABU62629.1.
AL663079 Genomic DNA. Translation: CAM19252.1. Sequence problems.
AL663079 Genomic DNA. Translation: CAM19253.1.
CH466558 Genomic DNA. Translation: EDL34441.1.
CCDSiCCDS36366.1. [A2AC93-1]
RefSeqiNP_001030050.2. NM_001034878.2. [A2AC93-1]
XP_006533723.1. XM_006533660.2. [A2AC93-1]
XP_006533724.1. XM_006533661.1. [A2AC93-1]
XP_006533725.1. XM_006533662.2. [A2AC93-1]
UniGeneiMm.212764.

3D structure databases

ProteinModelPortaliA2AC93.
SMRiA2AC93. Positions 228-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59778N.
STRINGi10090.ENSMUSP00000065787.

PTM databases

iPTMnetiA2AC93.
PhosphoSiteiA2AC93.

Proteomic databases

MaxQBiA2AC93.
PaxDbiA2AC93.
PRIDEiA2AC93.

Protocols and materials databases

DNASUi432611.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069325; ENSMUSP00000065787; ENSMUSG00000034706. [A2AC93-1]
ENSMUST00000092469; ENSMUSP00000090126; ENSMUSG00000034706. [A2AC93-1]
GeneIDi432611.
KEGGimmu:432611.
UCSCiuc007mfn.1. mouse. [A2AC93-1]

Organism-specific databases

CTDi432611.
MGIiMGI:2685574. Dnaic2.

Phylogenomic databases

eggNOGiENOG410IU37. Eukaryota.
ENOG410XTD6. LUCA.
GeneTreeiENSGT00840000129862.
HOGENOMiHOG000259651.
HOVERGENiHBG013035.
InParanoidiA2AC93.
KOiK11143.
OMAiETRGINH.
OrthoDBiEOG70GMF5.
PhylomeDBiA2AC93.
TreeFamiTF300553.

Miscellaneous databases

PROiA2AC93.
SOURCEiSearch...

Gene expression databases

BgeeiA2AC93.
ExpressionAtlasiA2AC93. baseline and differential.
GenevisibleiA2AC93. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse dynein axonemal intermediate chain 2: cloning and expression."
    Yang Z., Wu J.
    DNA Cell Biol. 27:479-488(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
    Tissue: Ovary.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: INTERACTION WITH DNAAF2.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  6. Cited for: INTERACTION WITH PIH1D3.

Entry informationi

Entry nameiDNAI2_MOUSE
AccessioniPrimary (citable) accession number: A2AC93
Secondary accession number(s): A2AC94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 3, 2009
Last modified: June 8, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.