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A2AB59 (RHG27_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 27
Alternative name(s):
CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Rho-type GTPase-activating protein 27
Gene names
Name:Arhgap27
Synonyms:Camgap1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length869 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 By similarity.

Subunit structure

Interacts with SH3KBP1/CIN85 By similarity.

Subcellular location

Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.

Tissue specificity

Widely expressed. Highly expressed in kidney, lung, small intestine and thymus. Ref.4

Sequence similarities

Contains 1 PH domain.

Contains 1 Rho-GAP domain.

Contains 1 SH3 domain.

Contains 3 WW domains.

Sequence caution

The sequence AAH96671.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A2AB59-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A2AB59-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-217: MAADVEGDVY...RCPPRAESPK → MVDMISKLVRRQSRALRA
Note: No experimental confirmation available. Contains a phosphotyrosine at position 28.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 869869Rho GTPase-activating protein 27
PRO_0000317579

Regions

Domain8 – 6962SH3
Domain246 – 28035WW 1
Domain299 – 33335WW 2
Domain414 – 44734WW 3
Domain477 – 593117PH
Domain677 – 866190Rho-GAP

Amino acid modifications

Modified residue2151Phosphoserine Ref.5

Natural variations

Alternative sequence1 – 217217MAADV…AESPK → MVDMISKLVRRQSRALRA in isoform 2.
VSP_031057

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: F53EDBAFE1B2A6E7

FASTA86997,049
        10         20         30         40         50         60 
MAADVEGDVY VLVEHPFEYT GKDGRRIAIQ PNERYRLLRR STEHWWHVRR EPGGRPFYLP 

        70         80         90        100        110        120 
AQYVRELPAL GDPAPAPQPS VPQQRPAVPE PLAYDYRFVS TPVGADGSSA EPRGRASSLC 

       130        140        150        160        170        180 
GPARQRTGGQ RNSLAPGGPA CLYVRPAAPV RPAQSLDDLA RGGTAPPAGL LGSAGHFKAS 

       190        200        210        220        230        240 
SVAGSWVCPR PLAPSDSENV YEAIPDLRCP PRAESPKQVD DPPEPVYANV ERQPRATSPR 

       250        260        270        280        290        300 
SAAAPPRLSP VWETHTDTGT GRPYYYNPDT GVTTWESPFE TPEGTTSPAT SRASVGSGES 

       310        320        330        340        350        360 
LETEWGQYWD EESRRVFFYN PLTGETAWED ETEELEEDHQ EQLEMQPSLS PRSPGQQRPP 

       370        380        390        400        410        420 
TPETDYPELL ASYPEEDYSP VGSFSDPGPA SPLVAPPGWS CQITPDKQML YTNQFTQEQW 

       430        440        450        460        470        480 
VRLEDQHGKP YFYNPEDSSV QWELPQVPIP APRSVRKSSQ DSDTPAQASP PEEKIKTLDK 

       490        500        510        520        530        540 
AGVLHRTKTV DKGKRLRKKH WSTSWTVLEG GVLTFFKDSK TSAAGGLRQP SKLSTPEYTV 

       550        560        570        580        590        600 
ELKGASLSWA PKDKSSKKNV LELRSRDGSE YLIQHDSEAI ISTWHKAIAE GISELSADLL 

       610        620        630        640        650        660 
QGEEGEPSSA DFGSSERLGS WREEDVRQNA ASPSLSPGGL ESDLSRVRHK LRKFLQRRPT 

       670        680        690        700        710        720 
LQSLRDKGYI KDQVFGCALA QLCERERSPV PRFVQQCIRT VEARGLDIDG LYRISGNLAT 

       730        740        750        760        770        780 
IQKLRYKVDH DERLDLDDGR WEDVHVITGA LKLFFRELPE PLFPFSHFHQ FIAAIKLQDP 

       790        800        810        820        830        840 
AQRSRCVRDL VRTLPAPNQD TLRLLIQHLC RVIEHGEQNR MTVQNVAIVF GPTLLRPEME 

       850        860 
EASMPMTMVF QNQVVELILH QCADIFPPH 

« Hide

Isoform 2 [UniParc].

Checksum: EC550B4E2ADCCE9B
Show »

FASTA67075,802

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 749-869.
Strain: Czech II.
Tissue: Lung.
[4]"Identification and characterization of a novel Rho GTPase activating protein implicated in receptor-mediated endocytosis."
Sakakibara T., Nemoto Y., Nukiwa T., Takeshima H.
FEBS Lett. 566:294-300(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-28 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK046763 mRNA. Translation: BAE20660.1.
AL772325, AL662804 Genomic DNA. Translation: CAM16599.1.
AL662804, AL772325 Genomic DNA. Translation: CAM21566.1.
BC096671 mRNA. Translation: AAH96671.1. Different initiation.
CCDSCCDS25517.1. [A2AB59-2]
CCDS56815.1. [A2AB59-1]
RefSeqNP_001192165.1. NM_001205236.1. [A2AB59-1]
NP_598476.2. NM_133715.5. [A2AB59-2]
UniGeneMm.259113.
Mm.397197.

3D structure databases

ProteinModelPortalA2AB59.
SMRA2AB59. Positions 7-67, 479-592, 658-866.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteA2AB59.

Proteomic databases

MaxQBA2AB59.
PaxDbA2AB59.
PRIDEA2AB59.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000041385; ENSMUSP00000039427; ENSMUSG00000034255. [A2AB59-2]
ENSMUST00000107024; ENSMUSP00000102639; ENSMUSG00000034255. [A2AB59-1]
GeneID544817.
KEGGmmu:544817.
UCSCuc007luc.2. mouse. [A2AB59-1]
uc007lud.2. mouse. [A2AB59-2]

Organism-specific databases

CTD201176.
MGIMGI:1916903. Arhgap27.

Phylogenomic databases

eggNOGNOG279563.
GeneTreeENSGT00730000110641.
HOGENOMHOG000294167.
HOVERGENHBG005328.
InParanoidA2AB59.
OMATPEYTVD.
OrthoDBEOG7CZK7R.
PhylomeDBA2AB59.
TreeFamTF329345.

Gene expression databases

ArrayExpressA2AB59.
BgeeA2AB59.
CleanExMM_ARHGAP27.
GenevestigatorA2AB59.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERPTHR23181. PTHR23181. 1 hit.
PfamPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50998. SSF50998. 2 hits.
SSF51045. SSF51045. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARHGAP27. mouse.
NextBio412048.
PROA2AB59.
SOURCESearch...

Entry information

Entry nameRHG27_MOUSE
AccessionPrimary (citable) accession number: A2AB59
Secondary accession number(s): Q3V366, Q4V9V5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot