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A2AB59

- RHG27_MOUSE

UniProt

A2AB59 - RHG27_MOUSE

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Protein

Rho GTPase-activating protein 27

Gene

Arhgap27

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).By similarity

GO - Molecular functioni

  1. Rac GTPase activator activity Source: UniProtKB
  2. SH3 domain binding Source: UniProtKB

GO - Biological processi

  1. positive regulation of Cdc42 GTPase activity Source: UniProtKB
  2. positive regulation of Rac GTPase activity Source: GOC
  3. receptor-mediated endocytosis Source: UniProtKB
  4. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 27
Alternative name(s):
CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Rho-type GTPase-activating protein 27
Gene namesi
Name:Arhgap27
Synonyms:Camgap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1916903. Arhgap27.

Subcellular locationi

Cytoplasm By similarity. Membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. intracellular Source: UniProtKB
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Rho GTPase-activating protein 27PRO_0000317579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA2AB59.
PaxDbiA2AB59.
PRIDEiA2AB59.

PTM databases

PhosphoSiteiA2AB59.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in kidney, lung, small intestine and thymus.1 Publication

Gene expression databases

BgeeiA2AB59.
CleanExiMM_ARHGAP27.
ExpressionAtlasiA2AB59. baseline and differential.
GenevestigatoriA2AB59.

Interactioni

Subunit structurei

Interacts with SH3KBP1/CIN85.By similarity

Structurei

3D structure databases

ProteinModelPortaliA2AB59.
SMRiA2AB59. Positions 7-67, 479-592, 658-866.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 6962SH3PROSITE-ProRule annotationAdd
BLAST
Domaini246 – 28035WW 1PROSITE-ProRule annotationAdd
BLAST
Domaini299 – 33335WW 2PROSITE-ProRule annotationAdd
BLAST
Domaini414 – 44734WW 3PROSITE-ProRule annotationAdd
BLAST
Domaini477 – 593117PHPROSITE-ProRule annotationAdd
BLAST
Domaini677 – 866190Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 3 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG279563.
GeneTreeiENSGT00760000118882.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiA2AB59.
OMAiTPEYTVD.
OrthoDBiEOG7CZK7R.
PhylomeDBiA2AB59.
TreeFamiTF329345.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 1 hit.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50998. SSF50998. 2 hits.
SSF51045. SSF51045. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A2AB59-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAADVEGDVY VLVEHPFEYT GKDGRRIAIQ PNERYRLLRR STEHWWHVRR
60 70 80 90 100
EPGGRPFYLP AQYVRELPAL GDPAPAPQPS VPQQRPAVPE PLAYDYRFVS
110 120 130 140 150
TPVGADGSSA EPRGRASSLC GPARQRTGGQ RNSLAPGGPA CLYVRPAAPV
160 170 180 190 200
RPAQSLDDLA RGGTAPPAGL LGSAGHFKAS SVAGSWVCPR PLAPSDSENV
210 220 230 240 250
YEAIPDLRCP PRAESPKQVD DPPEPVYANV ERQPRATSPR SAAAPPRLSP
260 270 280 290 300
VWETHTDTGT GRPYYYNPDT GVTTWESPFE TPEGTTSPAT SRASVGSGES
310 320 330 340 350
LETEWGQYWD EESRRVFFYN PLTGETAWED ETEELEEDHQ EQLEMQPSLS
360 370 380 390 400
PRSPGQQRPP TPETDYPELL ASYPEEDYSP VGSFSDPGPA SPLVAPPGWS
410 420 430 440 450
CQITPDKQML YTNQFTQEQW VRLEDQHGKP YFYNPEDSSV QWELPQVPIP
460 470 480 490 500
APRSVRKSSQ DSDTPAQASP PEEKIKTLDK AGVLHRTKTV DKGKRLRKKH
510 520 530 540 550
WSTSWTVLEG GVLTFFKDSK TSAAGGLRQP SKLSTPEYTV ELKGASLSWA
560 570 580 590 600
PKDKSSKKNV LELRSRDGSE YLIQHDSEAI ISTWHKAIAE GISELSADLL
610 620 630 640 650
QGEEGEPSSA DFGSSERLGS WREEDVRQNA ASPSLSPGGL ESDLSRVRHK
660 670 680 690 700
LRKFLQRRPT LQSLRDKGYI KDQVFGCALA QLCERERSPV PRFVQQCIRT
710 720 730 740 750
VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA
760 770 780 790 800
LKLFFRELPE PLFPFSHFHQ FIAAIKLQDP AQRSRCVRDL VRTLPAPNQD
810 820 830 840 850
TLRLLIQHLC RVIEHGEQNR MTVQNVAIVF GPTLLRPEME EASMPMTMVF
860
QNQVVELILH QCADIFPPH
Length:869
Mass (Da):97,049
Last modified:February 20, 2007 - v1
Checksum:iF53EDBAFE1B2A6E7
GO
Isoform 2 (identifier: A2AB59-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-217: MAADVEGDVY...RCPPRAESPK → MVDMISKLVRRQSRALRA

Note: No experimental confirmation available. Contains a phosphotyrosine at position 28.

Show »
Length:670
Mass (Da):75,802
Checksum:iEC550B4E2ADCCE9B
GO

Sequence cautioni

The sequence AAH96671.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 217217MAADV…AESPK → MVDMISKLVRRQSRALRA in isoform 2. 1 PublicationVSP_031057Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046763 mRNA. Translation: BAE20660.1.
AL772325, AL662804 Genomic DNA. Translation: CAM16599.1.
AL662804, AL772325 Genomic DNA. Translation: CAM21566.1.
BC096671 mRNA. Translation: AAH96671.1. Different initiation.
CCDSiCCDS25517.1. [A2AB59-2]
CCDS56815.1. [A2AB59-1]
RefSeqiNP_001192165.1. NM_001205236.1. [A2AB59-1]
NP_598476.2. NM_133715.5. [A2AB59-2]
UniGeneiMm.259113.
Mm.397197.

Genome annotation databases

EnsembliENSMUST00000041385; ENSMUSP00000039427; ENSMUSG00000034255. [A2AB59-2]
ENSMUST00000107024; ENSMUSP00000102639; ENSMUSG00000034255. [A2AB59-1]
GeneIDi544817.
KEGGimmu:544817.
UCSCiuc007luc.2. mouse. [A2AB59-1]
uc007lud.2. mouse. [A2AB59-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046763 mRNA. Translation: BAE20660.1 .
AL772325 , AL662804 Genomic DNA. Translation: CAM16599.1 .
AL662804 , AL772325 Genomic DNA. Translation: CAM21566.1 .
BC096671 mRNA. Translation: AAH96671.1 . Different initiation.
CCDSi CCDS25517.1. [A2AB59-2 ]
CCDS56815.1. [A2AB59-1 ]
RefSeqi NP_001192165.1. NM_001205236.1. [A2AB59-1 ]
NP_598476.2. NM_133715.5. [A2AB59-2 ]
UniGenei Mm.259113.
Mm.397197.

3D structure databases

ProteinModelPortali A2AB59.
SMRi A2AB59. Positions 7-67, 479-592, 658-866.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei A2AB59.

Proteomic databases

MaxQBi A2AB59.
PaxDbi A2AB59.
PRIDEi A2AB59.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041385 ; ENSMUSP00000039427 ; ENSMUSG00000034255 . [A2AB59-2 ]
ENSMUST00000107024 ; ENSMUSP00000102639 ; ENSMUSG00000034255 . [A2AB59-1 ]
GeneIDi 544817.
KEGGi mmu:544817.
UCSCi uc007luc.2. mouse. [A2AB59-1 ]
uc007lud.2. mouse. [A2AB59-2 ]

Organism-specific databases

CTDi 201176.
MGIi MGI:1916903. Arhgap27.

Phylogenomic databases

eggNOGi NOG279563.
GeneTreei ENSGT00760000118882.
HOGENOMi HOG000294167.
HOVERGENi HBG005328.
InParanoidi A2AB59.
OMAi TPEYTVD.
OrthoDBi EOG7CZK7R.
PhylomeDBi A2AB59.
TreeFami TF329345.

Miscellaneous databases

ChiTaRSi Arhgap27. mouse.
NextBioi 412048.
PROi A2AB59.
SOURCEi Search...

Gene expression databases

Bgeei A2AB59.
CleanExi MM_ARHGAP27.
ExpressionAtlasi A2AB59. baseline and differential.
Genevestigatori A2AB59.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view ]
PANTHERi PTHR23181. PTHR23181. 1 hit.
Pfami PF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
SM00456. WW. 3 hits.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50998. SSF50998. 2 hits.
SSF51045. SSF51045. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 749-869.
    Strain: Czech II.
    Tissue: Lung.
  4. "Identification and characterization of a novel Rho GTPase activating protein implicated in receptor-mediated endocytosis."
    Sakakibara T., Nemoto Y., Nukiwa T., Takeshima H.
    FEBS Lett. 566:294-300(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-28 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiRHG27_MOUSE
AccessioniPrimary (citable) accession number: A2AB59
Secondary accession number(s): Q3V366, Q4V9V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3