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A2AB59

- RHG27_MOUSE

UniProt

A2AB59 - RHG27_MOUSE

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Protein

Rho GTPase-activating protein 27

Gene
Arhgap27, Camgap1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 By similarity.

GO - Molecular functioni

  1. Rac GTPase activator activity Source: UniProtKB
  2. SH3 domain binding Source: UniProtKB

GO - Biological processi

  1. positive regulation of Cdc42 GTPase activity Source: UniProtKB
  2. positive regulation of Rac GTPase activity Source: GOC
  3. receptor-mediated endocytosis Source: UniProtKB
  4. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 27
Alternative name(s):
CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Rho-type GTPase-activating protein 27
Gene namesi
Name:Arhgap27
Synonyms:Camgap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1916903. Arhgap27.

Subcellular locationi

Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. intracellular Source: UniProtKB
  3. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 869869Rho GTPase-activating protein 27PRO_0000317579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA2AB59.
PaxDbiA2AB59.
PRIDEiA2AB59.

PTM databases

PhosphoSiteiA2AB59.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in kidney, lung, small intestine and thymus.1 Publication

Gene expression databases

ArrayExpressiA2AB59.
BgeeiA2AB59.
CleanExiMM_ARHGAP27.
GenevestigatoriA2AB59.

Interactioni

Subunit structurei

Interacts with SH3KBP1/CIN85 By similarity.

Structurei

3D structure databases

ProteinModelPortaliA2AB59.
SMRiA2AB59. Positions 7-67, 479-592, 658-866.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 6962SH3Add
BLAST
Domaini246 – 28035WW 1Add
BLAST
Domaini299 – 33335WW 2Add
BLAST
Domaini414 – 44734WW 3Add
BLAST
Domaini477 – 593117PHAdd
BLAST
Domaini677 – 866190Rho-GAPAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Contains 3 WW domains.

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiNOG279563.
GeneTreeiENSGT00730000110641.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiA2AB59.
OMAiTPEYTVD.
OrthoDBiEOG7CZK7R.
PhylomeDBiA2AB59.
TreeFamiTF329345.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 1 hit.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
SM00456. WW. 3 hits.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50998. SSF50998. 2 hits.
SSF51045. SSF51045. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A2AB59-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAADVEGDVY VLVEHPFEYT GKDGRRIAIQ PNERYRLLRR STEHWWHVRR    50
EPGGRPFYLP AQYVRELPAL GDPAPAPQPS VPQQRPAVPE PLAYDYRFVS 100
TPVGADGSSA EPRGRASSLC GPARQRTGGQ RNSLAPGGPA CLYVRPAAPV 150
RPAQSLDDLA RGGTAPPAGL LGSAGHFKAS SVAGSWVCPR PLAPSDSENV 200
YEAIPDLRCP PRAESPKQVD DPPEPVYANV ERQPRATSPR SAAAPPRLSP 250
VWETHTDTGT GRPYYYNPDT GVTTWESPFE TPEGTTSPAT SRASVGSGES 300
LETEWGQYWD EESRRVFFYN PLTGETAWED ETEELEEDHQ EQLEMQPSLS 350
PRSPGQQRPP TPETDYPELL ASYPEEDYSP VGSFSDPGPA SPLVAPPGWS 400
CQITPDKQML YTNQFTQEQW VRLEDQHGKP YFYNPEDSSV QWELPQVPIP 450
APRSVRKSSQ DSDTPAQASP PEEKIKTLDK AGVLHRTKTV DKGKRLRKKH 500
WSTSWTVLEG GVLTFFKDSK TSAAGGLRQP SKLSTPEYTV ELKGASLSWA 550
PKDKSSKKNV LELRSRDGSE YLIQHDSEAI ISTWHKAIAE GISELSADLL 600
QGEEGEPSSA DFGSSERLGS WREEDVRQNA ASPSLSPGGL ESDLSRVRHK 650
LRKFLQRRPT LQSLRDKGYI KDQVFGCALA QLCERERSPV PRFVQQCIRT 700
VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA 750
LKLFFRELPE PLFPFSHFHQ FIAAIKLQDP AQRSRCVRDL VRTLPAPNQD 800
TLRLLIQHLC RVIEHGEQNR MTVQNVAIVF GPTLLRPEME EASMPMTMVF 850
QNQVVELILH QCADIFPPH 869
Length:869
Mass (Da):97,049
Last modified:February 20, 2007 - v1
Checksum:iF53EDBAFE1B2A6E7
GO
Isoform 2 (identifier: A2AB59-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-217: MAADVEGDVY...RCPPRAESPK → MVDMISKLVRRQSRALRA

Note: No experimental confirmation available. Contains a phosphotyrosine at position 28.

Show »
Length:670
Mass (Da):75,802
Checksum:iEC550B4E2ADCCE9B
GO

Sequence cautioni

The sequence AAH96671.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 217217MAADV…AESPK → MVDMISKLVRRQSRALRA in isoform 2. VSP_031057Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK046763 mRNA. Translation: BAE20660.1.
AL772325, AL662804 Genomic DNA. Translation: CAM16599.1.
AL662804, AL772325 Genomic DNA. Translation: CAM21566.1.
BC096671 mRNA. Translation: AAH96671.1. Different initiation.
CCDSiCCDS25517.1. [A2AB59-2]
CCDS56815.1. [A2AB59-1]
RefSeqiNP_001192165.1. NM_001205236.1. [A2AB59-1]
NP_598476.2. NM_133715.5. [A2AB59-2]
UniGeneiMm.259113.
Mm.397197.

Genome annotation databases

EnsembliENSMUST00000041385; ENSMUSP00000039427; ENSMUSG00000034255. [A2AB59-2]
ENSMUST00000107024; ENSMUSP00000102639; ENSMUSG00000034255. [A2AB59-1]
GeneIDi544817.
KEGGimmu:544817.
UCSCiuc007luc.2. mouse. [A2AB59-1]
uc007lud.2. mouse. [A2AB59-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK046763 mRNA. Translation: BAE20660.1 .
AL772325 , AL662804 Genomic DNA. Translation: CAM16599.1 .
AL662804 , AL772325 Genomic DNA. Translation: CAM21566.1 .
BC096671 mRNA. Translation: AAH96671.1 . Different initiation.
CCDSi CCDS25517.1. [A2AB59-2 ]
CCDS56815.1. [A2AB59-1 ]
RefSeqi NP_001192165.1. NM_001205236.1. [A2AB59-1 ]
NP_598476.2. NM_133715.5. [A2AB59-2 ]
UniGenei Mm.259113.
Mm.397197.

3D structure databases

ProteinModelPortali A2AB59.
SMRi A2AB59. Positions 7-67, 479-592, 658-866.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei A2AB59.

Proteomic databases

MaxQBi A2AB59.
PaxDbi A2AB59.
PRIDEi A2AB59.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041385 ; ENSMUSP00000039427 ; ENSMUSG00000034255 . [A2AB59-2 ]
ENSMUST00000107024 ; ENSMUSP00000102639 ; ENSMUSG00000034255 . [A2AB59-1 ]
GeneIDi 544817.
KEGGi mmu:544817.
UCSCi uc007luc.2. mouse. [A2AB59-1 ]
uc007lud.2. mouse. [A2AB59-2 ]

Organism-specific databases

CTDi 201176.
MGIi MGI:1916903. Arhgap27.

Phylogenomic databases

eggNOGi NOG279563.
GeneTreei ENSGT00730000110641.
HOGENOMi HOG000294167.
HOVERGENi HBG005328.
InParanoidi A2AB59.
OMAi TPEYTVD.
OrthoDBi EOG7CZK7R.
PhylomeDBi A2AB59.
TreeFami TF329345.

Miscellaneous databases

ChiTaRSi ARHGAP27. mouse.
NextBioi 412048.
PROi A2AB59.
SOURCEi Search...

Gene expression databases

ArrayExpressi A2AB59.
Bgeei A2AB59.
CleanExi MM_ARHGAP27.
Genevestigatori A2AB59.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view ]
PANTHERi PTHR23181. PTHR23181. 1 hit.
Pfami PF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
SM00456. WW. 3 hits.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50998. SSF50998. 2 hits.
SSF51045. SSF51045. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 749-869.
    Strain: Czech II.
    Tissue: Lung.
  4. "Identification and characterization of a novel Rho GTPase activating protein implicated in receptor-mediated endocytosis."
    Sakakibara T., Nemoto Y., Nukiwa T., Takeshima H.
    FEBS Lett. 566:294-300(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-28 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiRHG27_MOUSE
AccessioniPrimary (citable) accession number: A2AB59
Secondary accession number(s): Q3V366, Q4V9V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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