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A2AB59

- RHG27_MOUSE

UniProt

A2AB59 - RHG27_MOUSE

Protein

Rho GTPase-activating protein 27

Gene

Arhgap27

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 By similarity.By similarity

    GO - Molecular functioni

    1. Rac GTPase activator activity Source: UniProtKB
    2. SH3 domain binding Source: UniProtKB

    GO - Biological processi

    1. positive regulation of Cdc42 GTPase activity Source: UniProtKB
    2. positive regulation of Rac GTPase activity Source: GOC
    3. receptor-mediated endocytosis Source: UniProtKB
    4. signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Biological processi

    Endocytosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho GTPase-activating protein 27
    Alternative name(s):
    CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
    Rho-type GTPase-activating protein 27
    Gene namesi
    Name:Arhgap27
    Synonyms:Camgap1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1916903. Arhgap27.

    Subcellular locationi

    Cytoplasm By similarity. Membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. intracellular Source: UniProtKB
    3. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 869869Rho GTPase-activating protein 27PRO_0000317579Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei215 – 2151Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiA2AB59.
    PaxDbiA2AB59.
    PRIDEiA2AB59.

    PTM databases

    PhosphoSiteiA2AB59.

    Expressioni

    Tissue specificityi

    Widely expressed. Highly expressed in kidney, lung, small intestine and thymus.1 Publication

    Gene expression databases

    ArrayExpressiA2AB59.
    BgeeiA2AB59.
    CleanExiMM_ARHGAP27.
    GenevestigatoriA2AB59.

    Interactioni

    Subunit structurei

    Interacts with SH3KBP1/CIN85.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA2AB59.
    SMRiA2AB59. Positions 7-67, 479-592, 658-866.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 6962SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini246 – 28035WW 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini299 – 33335WW 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini414 – 44734WW 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini477 – 593117PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini677 – 866190Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation
    Contains 3 WW domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiNOG279563.
    GeneTreeiENSGT00730000110641.
    HOGENOMiHOG000294167.
    HOVERGENiHBG005328.
    InParanoidiA2AB59.
    OMAiTPEYTVD.
    OrthoDBiEOG7CZK7R.
    PhylomeDBiA2AB59.
    TreeFamiTF329345.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR015767. Rho_GTPase_act.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    IPR001202. WW_dom.
    [Graphical view]
    PANTHERiPTHR23181. PTHR23181. 1 hit.
    PfamiPF00169. PH. 1 hit.
    PF00620. RhoGAP. 1 hit.
    PF00397. WW. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    SM00456. WW. 3 hits.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF50998. SSF50998. 2 hits.
    SSF51045. SSF51045. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    PS50020. WW_DOMAIN_2. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A2AB59-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAADVEGDVY VLVEHPFEYT GKDGRRIAIQ PNERYRLLRR STEHWWHVRR    50
    EPGGRPFYLP AQYVRELPAL GDPAPAPQPS VPQQRPAVPE PLAYDYRFVS 100
    TPVGADGSSA EPRGRASSLC GPARQRTGGQ RNSLAPGGPA CLYVRPAAPV 150
    RPAQSLDDLA RGGTAPPAGL LGSAGHFKAS SVAGSWVCPR PLAPSDSENV 200
    YEAIPDLRCP PRAESPKQVD DPPEPVYANV ERQPRATSPR SAAAPPRLSP 250
    VWETHTDTGT GRPYYYNPDT GVTTWESPFE TPEGTTSPAT SRASVGSGES 300
    LETEWGQYWD EESRRVFFYN PLTGETAWED ETEELEEDHQ EQLEMQPSLS 350
    PRSPGQQRPP TPETDYPELL ASYPEEDYSP VGSFSDPGPA SPLVAPPGWS 400
    CQITPDKQML YTNQFTQEQW VRLEDQHGKP YFYNPEDSSV QWELPQVPIP 450
    APRSVRKSSQ DSDTPAQASP PEEKIKTLDK AGVLHRTKTV DKGKRLRKKH 500
    WSTSWTVLEG GVLTFFKDSK TSAAGGLRQP SKLSTPEYTV ELKGASLSWA 550
    PKDKSSKKNV LELRSRDGSE YLIQHDSEAI ISTWHKAIAE GISELSADLL 600
    QGEEGEPSSA DFGSSERLGS WREEDVRQNA ASPSLSPGGL ESDLSRVRHK 650
    LRKFLQRRPT LQSLRDKGYI KDQVFGCALA QLCERERSPV PRFVQQCIRT 700
    VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA 750
    LKLFFRELPE PLFPFSHFHQ FIAAIKLQDP AQRSRCVRDL VRTLPAPNQD 800
    TLRLLIQHLC RVIEHGEQNR MTVQNVAIVF GPTLLRPEME EASMPMTMVF 850
    QNQVVELILH QCADIFPPH 869
    Length:869
    Mass (Da):97,049
    Last modified:February 20, 2007 - v1
    Checksum:iF53EDBAFE1B2A6E7
    GO
    Isoform 2 (identifier: A2AB59-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-217: MAADVEGDVY...RCPPRAESPK → MVDMISKLVRRQSRALRA

    Note: No experimental confirmation available. Contains a phosphotyrosine at position 28.

    Show »
    Length:670
    Mass (Da):75,802
    Checksum:iEC550B4E2ADCCE9B
    GO

    Sequence cautioni

    The sequence AAH96671.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 217217MAADV…AESPK → MVDMISKLVRRQSRALRA in isoform 2. 1 PublicationVSP_031057Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK046763 mRNA. Translation: BAE20660.1.
    AL772325, AL662804 Genomic DNA. Translation: CAM16599.1.
    AL662804, AL772325 Genomic DNA. Translation: CAM21566.1.
    BC096671 mRNA. Translation: AAH96671.1. Different initiation.
    CCDSiCCDS25517.1. [A2AB59-2]
    CCDS56815.1. [A2AB59-1]
    RefSeqiNP_001192165.1. NM_001205236.1. [A2AB59-1]
    NP_598476.2. NM_133715.5. [A2AB59-2]
    UniGeneiMm.259113.
    Mm.397197.

    Genome annotation databases

    EnsembliENSMUST00000041385; ENSMUSP00000039427; ENSMUSG00000034255. [A2AB59-2]
    ENSMUST00000107024; ENSMUSP00000102639; ENSMUSG00000034255. [A2AB59-1]
    GeneIDi544817.
    KEGGimmu:544817.
    UCSCiuc007luc.2. mouse. [A2AB59-1]
    uc007lud.2. mouse. [A2AB59-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK046763 mRNA. Translation: BAE20660.1 .
    AL772325 , AL662804 Genomic DNA. Translation: CAM16599.1 .
    AL662804 , AL772325 Genomic DNA. Translation: CAM21566.1 .
    BC096671 mRNA. Translation: AAH96671.1 . Different initiation.
    CCDSi CCDS25517.1. [A2AB59-2 ]
    CCDS56815.1. [A2AB59-1 ]
    RefSeqi NP_001192165.1. NM_001205236.1. [A2AB59-1 ]
    NP_598476.2. NM_133715.5. [A2AB59-2 ]
    UniGenei Mm.259113.
    Mm.397197.

    3D structure databases

    ProteinModelPortali A2AB59.
    SMRi A2AB59. Positions 7-67, 479-592, 658-866.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei A2AB59.

    Proteomic databases

    MaxQBi A2AB59.
    PaxDbi A2AB59.
    PRIDEi A2AB59.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000041385 ; ENSMUSP00000039427 ; ENSMUSG00000034255 . [A2AB59-2 ]
    ENSMUST00000107024 ; ENSMUSP00000102639 ; ENSMUSG00000034255 . [A2AB59-1 ]
    GeneIDi 544817.
    KEGGi mmu:544817.
    UCSCi uc007luc.2. mouse. [A2AB59-1 ]
    uc007lud.2. mouse. [A2AB59-2 ]

    Organism-specific databases

    CTDi 201176.
    MGIi MGI:1916903. Arhgap27.

    Phylogenomic databases

    eggNOGi NOG279563.
    GeneTreei ENSGT00730000110641.
    HOGENOMi HOG000294167.
    HOVERGENi HBG005328.
    InParanoidi A2AB59.
    OMAi TPEYTVD.
    OrthoDBi EOG7CZK7R.
    PhylomeDBi A2AB59.
    TreeFami TF329345.

    Miscellaneous databases

    ChiTaRSi ARHGAP27. mouse.
    NextBioi 412048.
    PROi A2AB59.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A2AB59.
    Bgeei A2AB59.
    CleanExi MM_ARHGAP27.
    Genevestigatori A2AB59.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR015767. Rho_GTPase_act.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    IPR001202. WW_dom.
    [Graphical view ]
    PANTHERi PTHR23181. PTHR23181. 1 hit.
    Pfami PF00169. PH. 1 hit.
    PF00620. RhoGAP. 1 hit.
    PF00397. WW. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    SM00456. WW. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF50998. SSF50998. 2 hits.
    SSF51045. SSF51045. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    PS50020. WW_DOMAIN_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Thymus.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 749-869.
      Strain: Czech II.
      Tissue: Lung.
    4. "Identification and characterization of a novel Rho GTPase activating protein implicated in receptor-mediated endocytosis."
      Sakakibara T., Nemoto Y., Nukiwa T., Takeshima H.
      FEBS Lett. 566:294-300(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-28 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiRHG27_MOUSE
    AccessioniPrimary (citable) accession number: A2AB59
    Secondary accession number(s): Q3V366, Q4V9V5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3