ID   MAGB4_MOUSE             Reviewed;         556 AA.
AC   A2A9R3; B7ZWI9; Q3UNA3;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-FEB-2007, sequence version 1.
DT   24-JAN-2024, entry version 116.
DE   RecName: Full=Melanoma-associated antigen B4 {ECO:0000312|MGI:MGI:2148568};
GN   Name=Mageb4 {ECO:0000312|MGI:MGI:2148568};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3] {ECO:0000312|EMBL:EDL29148.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis {ECO:0000312|EMBL:AAI50913.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5] {ECO:0000305}
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=10706124;
RA   Osterlund C., Toehoenen V., Forslund K.O., Nordqvist K.;
RT   "Mage-b4, a novel melanoma antigen (MAGE) gene specifically expressed
RT   during germ cell differentiation.";
RL   Cancer Res. 60:1054-1061(2000).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-325, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10706124}.
CC   -!- TISSUE SPECIFICITY: Expressed in testis (at protein level).
CC       {ECO:0000269|PubMed:10706124}.
CC   -!- DEVELOPMENTAL STAGE: Detected between 13.5 and 14.5 dpc in both female
CC       and male gonads (at protein level). Increases in the male gonads and
CC       intensely expressed before birth (at protein level). In female gonads,
CC       remains constant until birth (at protein level). In testis,
CC       specifically expressed in premeiotic germ cells (at protein level). In
CC       fetal ovary, expressed in both premeiotic germ cells, and germ cells
CC       that have gone through the pachytene phase and entered meiotic arrest
CC       (at protein level). {ECO:0000269|PubMed:10706124}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK144342; BAE25842.1; -; mRNA.
DR   EMBL; AK144350; BAE25844.1; -; mRNA.
DR   EMBL; AL627411; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AL954720; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466576; EDL29148.1; -; Genomic_DNA.
DR   EMBL; BC150912; AAI50913.1; -; mRNA.
DR   EMBL; BC172073; AAI72073.1; -; mRNA.
DR   CCDS; CCDS30261.1; -.
DR   RefSeq; NP_001028664.2; NM_001033492.2.
DR   AlphaFoldDB; A2A9R3; -.
DR   SMR; A2A9R3; -.
DR   STRING; 10090.ENSMUSP00000109602; -.
DR   iPTMnet; A2A9R3; -.
DR   PhosphoSitePlus; A2A9R3; -.
DR   SwissPalm; A2A9R3; -.
DR   PaxDb; 10090-ENSMUSP00000109602; -.
DR   ProteomicsDB; 287296; -.
DR   Pumba; A2A9R3; -.
DR   Antibodypedia; 24654; 223 antibodies from 27 providers.
DR   Ensembl; ENSMUST00000096378.3; ENSMUSP00000094107.3; ENSMUSG00000035427.19.
DR   Ensembl; ENSMUST00000113969.10; ENSMUSP00000109602.4; ENSMUSG00000035427.19.
DR   GeneID; 434903; -.
DR   KEGG; mmu:434903; -.
DR   UCSC; uc009tse.1; mouse.
DR   AGR; MGI:2148568; -.
DR   CTD; 4115; -.
DR   MGI; MGI:2148568; Mageb4.
DR   VEuPathDB; HostDB:ENSMUSG00000035427; -.
DR   eggNOG; KOG4562; Eukaryota.
DR   GeneTree; ENSGT00940000155485; -.
DR   HOGENOM; CLU_489970_0_0_1; -.
DR   InParanoid; A2A9R3; -.
DR   OMA; NGNCARE; -.
DR   OrthoDB; 3128316at2759; -.
DR   PhylomeDB; A2A9R3; -.
DR   TreeFam; TF328505; -.
DR   BioGRID-ORCS; 434903; 3 hits in 78 CRISPR screens.
DR   ChiTaRS; Mageb4; mouse.
DR   PRO; PR:A2A9R3; -.
DR   Proteomes; UP000000589; Chromosome X.
DR   RNAct; A2A9R3; Protein.
DR   Bgee; ENSMUSG00000035427; Expressed in mesodermal cell in embryo and 6 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.1200; MAGE homology domain, winged helix WH1 motif; 1.
DR   Gene3D; 1.10.10.1210; MAGE homology domain, winged helix WH2 motif; 1.
DR   InterPro; IPR037445; MAGE.
DR   InterPro; IPR021072; MAGE_N.
DR   InterPro; IPR041898; MAGE_WH1.
DR   InterPro; IPR041899; MAGE_WH2.
DR   InterPro; IPR002190; MHD_dom.
DR   PANTHER; PTHR11736:SF22; MELANOMA-ASSOCIATED ANTIGEN B4; 1.
DR   PANTHER; PTHR11736; MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN; 1.
DR   Pfam; PF01454; MAGE; 1.
DR   Pfam; PF12440; MAGE_N; 1.
DR   SMART; SM01373; MAGE; 1.
DR   SMART; SM01392; MAGE_N; 1.
DR   PROSITE; PS50838; MAGE; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Phosphoprotein; Reference proteome; Repeat; Tumor antigen.
FT   CHAIN           1..556
FT                   /note="Melanoma-associated antigen B4"
FT                   /id="PRO_0000437174"
FT   DOMAIN          111..310
FT                   /note="MAGE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT   REPEAT          334..348
FT                   /note="1"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          349..363
FT                   /note="2"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          364..378
FT                   /note="3"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          379..392
FT                   /note="4"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          393..407
FT                   /note="5"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          408..421
FT                   /note="6"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          422..436
FT                   /note="7"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          437..451
FT                   /note="8"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          452..466
FT                   /note="9"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          467..480
FT                   /note="10"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          481..495
FT                   /note="11"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          496..510
FT                   /note="12"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          511..525
FT                   /note="13"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          526..539
FT                   /note="14"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REPEAT          540..554
FT                   /note="15"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   REGION          1..110
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          315..335
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          334..554
FT                   /note="15 X 15 AA approximate tandem repeats"
FT                   /evidence="ECO:0000303|PubMed:10706124"
FT   COMPBIAS        13..31
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        36..71
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        92..110
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         325
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   CONFLICT        78
FT                   /note="A -> T (in Ref. 4; AAI72073)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        378
FT                   /note="N -> K (in Ref. 1; BAE25842/BAE25844)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        444
FT                   /note="V -> L (in Ref. 1; BAE25842/BAE25844)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        529..533
FT                   /note="KVTLG -> NGHTLA (in Ref. 1; BAE25842/BAE25844)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   556 AA;  60922 MW;  A2B67EE2398651E0 CRC64;
     MPRGQKSKAR AREKRRLVQD EAQELQDAQA KAGEKGKSPS CSNQDSGDAV ASTSTAGFPQ
     KSKSQGEAPT TTARKGGACR RSRKSTRGPR EESTSCTRVP RFSENPQNDL LTRKTGMLMQ
     YLLCKYKMKQ PASKGEMLKV INRRFKEQLP EILKKASERI QLVFGLEVKE IKPNGGYYTL
     VSKLDPSVGN ALTTSLPFPQ NGLLMPLLGV IFLNGNRASE AEIWEFLNVL GIYDGKVHII
     FGEPRKLITR DLVKEKYLVY QKEANSDPPS FEFLWGPRAY AETTKMKILE FLAEVNETVP
     QAFPTHYEEA LRDQEERAQA EAVGSPGTSA KDKAEAKVTL VDSSCKYQAE SKVTLVDSSC
     KDQAESKVTL VDPSCKDNAK SKVTLGSSRK YKAKSKVPLV DSSCKDQAES KVTLVDSCKD
     QAESKVTLVD SSCKDQAESK VTLVDSSCKD QAESKVTLVD PSCKDKAKSK VTLGSSHKYK
     AKSKVTLVDS SCKDQAESKV TLVDSSCKDQ AESKVTLVDP SCKDNAKSKV TLGSSRKYKA
     KSKVPLVDSS GKDKAV
//