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Protein

Activation-dependent, raft-recruited ADAP-like phosphoprotein

Gene

ARAP

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that plays a role in T-cell receptor (TCR)-mediated activation of signaling pathways. Required for T-cell activation and integrin-mediated T-cell adhesion in response to TCR stimulation.By similarity

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Activation-dependent, raft-recruited ADAP-like phosphoprotein
Gene namesi
Name:ARAP
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2685466. 1700024P16Rik.

Subcellular locationi

  • Membrane raft By similarity

  • Note: Recruited to membrane rafts and immunological synapse after TCR stimulation.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003045841 – 729Activation-dependent, raft-recruited ADAP-like phosphoproteinAdd BLAST729

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei489PhosphotyrosineBy similarity1
Modified residuei591PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylation is required for its function in T-cell activation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA2A995.
PeptideAtlasiA2A995.
PRIDEiA2A995.

PTM databases

iPTMnetiA2A995.
PhosphoSitePlusiA2A995.

Expressioni

Gene expression databases

BgeeiENSMUSG00000078612.
CleanExiMM_1700024P16RIK.
ExpressionAtlasiA2A995. baseline and differential.

Interactioni

Subunit structurei

Interacts with SKAP1, LCK and FYN. The phosphorylated form interacts with LCP2.By similarity

Structurei

3D structure databases

ProteinModelPortaliA2A995.
SMRiA2A995.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini653 – 720SH3Add BLAST68

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi520 – 523SH2-binding; to LCP2By similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi91 – 94Poly-SerSequence analysis4
Compositional biasi276 – 281Poly-ProSequence analysis6
Compositional biasi481 – 485Poly-SerSequence analysis5

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410IFNY. Eukaryota.
ENOG41116CN. LUCA.
GeneTreeiENSGT00730000111334.
HOVERGENiHBG106641.
InParanoidiA2A995.
PhylomeDBiA2A995.

Family and domain databases

InterProiView protein in InterPro
IPR029294. hSH3.
IPR001452. SH3_domain.
PfamiView protein in Pfam
PF14603. hSH3. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.

Sequencei

Sequence statusi: Complete.

A2A995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEGIRNFK ELRAKFQKFN AAPLPGPMRF PAGVSRKHDR GSTQPAQDLA
60 70 80 90 100
NRKCLSSHHH QTPSHSSTGV SQPLKNQTLK SAQGDELQKT SSSSGTPEKS
110 120 130 140 150
TVCPERDFQK AAVPLDVTKS RAKTSNEEKG MTLSSFRYKL WNWEKVSSQN
160 170 180 190 200
GEMSPAPLLT NGGIKTFHFE GQKTMGLAQD KPEKSLKATG AQTLPPQSHS
210 220 230 240 250
MAQRKSPVTS KASSVSLPPH SRKSTKSPAT EGSHRSSQCQ PVYECELDSL
260 270 280 290 300
VPEKPQSRHC RLPKTKPLPS IETLGPPPPK PSKPPFVNLY AFHRLPATVT
310 320 330 340 350
KTPKEETMKE GPLSPDSAEL EEAHNYDTTI SYLRHSGNSI NLCAEGESTE
360 370 380 390 400
ATYEIEIEEL QKPWRSFFLP ELSPRPKEEE NTMEEKESWE SEPLEPRKEL
410 420 430 440 450
HPSRPPKVVV YKETPGKTQM AGVHEDRRSV PAGNQEAMTD IMQNRLFPED
460 470 480 490 500
VTLTRHSQDK SGYVEALEVT KETPSPSTIR SSSSSEKTYD AVECSREDVR
510 520 530 540 550
KWDFSSSFTS DSEENCEEMY EDIYKAKNDD PKTEVAGRTA LRKLQQIFRK
560 570 580 590 600
ENVVFRMKKT KSKEIVSNGF SVSLPDLGPR SQDDSQDGII YDDVDTREKE
610 620 630 640 650
SNDEDKVKTW KTKFLIPKGK KWGKGSQGSK SFSPRHFFRT KKQKLEKNRM
660 670 680 690 700
EKEEKLFRER FQYGKEILVI NRAVACASNS RNGMFDLPII PGEQLEVIDT
710 720
TEQNLVICRN SKGKYGYVLV EHLDFKHQG
Length:729
Mass (Da):82,296
Last modified:February 20, 2007 - v1
Checksum:i62CDE8137EA7138A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti523I → V in AK028077 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028077 mRNA. No translation available.
AL627186, AL929466 Genomic DNA. Translation: CAM23654.1.
AL929466, AL627186 Genomic DNA. Translation: CAM24128.1.
UniGeneiMm.65181.

Genome annotation databases

EnsembliENSMUST00000106804; ENSMUSP00000102416; ENSMUSG00000078612.
UCSCiuc012dhy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028077 mRNA. No translation available.
AL627186, AL929466 Genomic DNA. Translation: CAM23654.1.
AL929466, AL627186 Genomic DNA. Translation: CAM24128.1.
UniGeneiMm.65181.

3D structure databases

ProteinModelPortaliA2A995.
SMRiA2A995.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiA2A995.
PhosphoSitePlusiA2A995.

Proteomic databases

PaxDbiA2A995.
PeptideAtlasiA2A995.
PRIDEiA2A995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106804; ENSMUSP00000102416; ENSMUSG00000078612.
UCSCiuc012dhy.1. mouse.

Organism-specific databases

MGIiMGI:2685466. 1700024P16Rik.

Phylogenomic databases

eggNOGiENOG410IFNY. Eukaryota.
ENOG41116CN. LUCA.
GeneTreeiENSGT00730000111334.
HOVERGENiHBG106641.
InParanoidiA2A995.
PhylomeDBiA2A995.

Miscellaneous databases

PROiPR:A2A995.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078612.
CleanExiMM_1700024P16RIK.
ExpressionAtlasiA2A995. baseline and differential.

Family and domain databases

InterProiView protein in InterPro
IPR029294. hSH3.
IPR001452. SH3_domain.
PfamiView protein in Pfam
PF14603. hSH3. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARAP_MOUSE
AccessioniPrimary (citable) accession number: A2A995
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 20, 2007
Last modified: April 12, 2017
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.