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Protein

Receptor-type tyrosine-protein phosphatase F

Gene

Ptprf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1507SubstrateBy similarity1
Active sitei1539Phosphocysteine intermediateBy similarity1
Binding sitei1583SubstrateBy similarity1
Active sitei1830Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

  • chondroitin sulfate proteoglycan binding Source: MGI
  • heparin binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: MGI
  • protein complex binding Source: MGI
  • protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • cell migration Source: UniProtKB
  • negative regulation of receptor binding Source: UniProtKB
  • neuron projection regeneration Source: MGI
  • peptidyl-tyrosine dephosphorylation Source: UniProtKB
  • regulation of axon regeneration Source: MGI
  • regulation of neuron projection development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase F (EC:3.1.3.48)
Alternative name(s):
Leukocyte common antigen related
Short name:
LAR
Gene namesi
Name:Ptprf
Synonyms:Lar
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:102695. Ptprf.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 1254ExtracellularSequence analysisAdd BLAST1225
Transmembranei1255 – 1275HelicalSequence analysisAdd BLAST21
Topological domaini1276 – 1898CytoplasmicSequence analysisAdd BLAST623

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000037019330 – 1898Receptor-type tyrosine-protein phosphatase FAdd BLAST1869

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 107PROSITE-ProRule annotation1 Publication
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi156 ↔ 207PROSITE-ProRule annotation1 Publication
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi253 ↔ 298PROSITE-ProRule annotation
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Glycosylationi721N-linked (GlcNAc...)1 Publication1
Glycosylationi936N-linked (GlcNAc...); atypical1 Publication1
Glycosylationi941N-linked (GlcNAc...)1 Publication1
Glycosylationi957N-linked (GlcNAc...)Sequence analysis1
Glycosylationi960N-linked (GlcNAc...)1 Publication1
Modified residuei1296PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiA2A8L5.
PaxDbiA2A8L5.
PeptideAtlasiA2A8L5.
PRIDEiA2A8L5.

PTM databases

iPTMnetiA2A8L5.
PhosphoSitePlusiA2A8L5.
SwissPalmiA2A8L5.

Expressioni

Tissue specificityi

Expressed in the cell of the T lineage but not in cells of any other hemopoietic lineage.1 Publication

Gene expression databases

BgeeiENSMUSG00000033295.
ExpressionAtlasiA2A8L5. baseline and differential.
GenevisibleiA2A8L5. MM.

Interactioni

Subunit structurei

Interacts with GRIP1. Interacts with PPFIA1, PPFIA2 and PPFIA3. Interacts with PTPRF.By similarity

GO - Molecular functioni

  • protein complex binding Source: MGI

Protein-protein interaction databases

BioGridi202497. 4 interactors.
IntActiA2A8L5. 1 interactor.
STRINGi10090.ENSMUSP00000039368.

Structurei

Secondary structure

11898
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi31 – 37Combined sources7
Beta strandi42 – 45Combined sources4
Beta strandi50 – 60Combined sources11
Beta strandi63 – 68Combined sources6
Beta strandi78 – 83Combined sources6
Helixi84 – 86Combined sources3
Beta strandi88 – 93Combined sources6
Helixi98 – 101Combined sources4
Beta strandi103 – 110Combined sources8
Beta strandi115 – 125Combined sources11
Beta strandi136 – 139Combined sources4
Beta strandi144 – 146Combined sources3
Beta strandi148 – 150Combined sources3
Beta strandi152 – 154Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi165 – 170Combined sources6
Turni177 – 179Combined sources3
Beta strandi180 – 186Combined sources7
Beta strandi192 – 194Combined sources3
Helixi199 – 201Combined sources3
Beta strandi203 – 211Combined sources9
Beta strandi214 – 217Combined sources4
Beta strandi221 – 225Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PXHX-ray2.00A30-226[»]
ProteinModelPortaliA2A8L5.
SMRiA2A8L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 123Ig-like C2-type 1Add BLAST91
Domaini135 – 224Ig-like C2-type 2Add BLAST90
Domaini232 – 314Ig-like C2-type 3Add BLAST83
Domaini321 – 411Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST91
Domaini416 – 510Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST95
Domaini514 – 604Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST91
Domaini609 – 706Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST98
Domaini711 – 810Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST100
Domaini811 – 904Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST94
Domaini909 – 1001Fibronectin type-III 7PROSITE-ProRule annotationAdd BLAST93
Domaini1005 – 1089Fibronectin type-III 8PROSITE-ProRule annotationAdd BLAST85
Domaini1343 – 1598Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST256
Domaini1630 – 1889Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST260

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 77Heparin-binding10
Regioni1539 – 1545Substrate bindingBy similarity7

Domaini

The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.By similarity

Sequence similaritiesi

Contains 8 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000010250.
InParanoidiA2A8L5.
KOiK05695.
OMAiPSAWHKH.
OrthoDBiEOG091G11WG.
PhylomeDBiA2A8L5.
TreeFamiTF312900.

Family and domain databases

CDDicd00063. FN3. 8 hits.
Gene3Di2.60.40.10. 11 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 8 hits.
SM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
SSF49265. SSF49265. 5 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2A8L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPEPAPGRR MVPLVPALVM LGLMAGAHGD SKPVFVKVPE DQTGLSGGVA
60 70 80 90 100
SFVCQATGEP KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR
110 120 130 140 150
DEAIYECTAT NSLGEINTSA KLSVLEEDQL PSGFPTIDMG PQLKVVEKGR
160 170 180 190 200
TATMLCAAGG NPDPEISWFK DFLPVDPAAS NGRIKQLRSG ALQIESSEES
210 220 230 240 250
DQGKYECVAT NSAGTRYSAP ANLYVRVRRV APRFSIPPSS QEVMPGGSVN
260 270 280 290 300
LTCVAVGAPM PYVKWMMGAE ELTKEDEMPV GRNVLELSNV MRSANYTCVA
310 320 330 340 350
ISSLGMIEAT AQVTVKALPK PPIDLVVTET TATSVTLTWD SGNTEPVSFY
360 370 380 390 400
GIQYRAAGTD GPFQEVDGVA STRYSIGGLS PFSEYAFRVL AVNSIGRGPP
410 420 430 440 450
SEAVRARTGE QAPSSPPRRV QARMLSASTM LVQWEPPEEP NGLVRGYRVY
460 470 480 490 500
YTPDSRRPLS AWHKHNTDAG LLTTVGSLLP GITYSLRVLA FTAVGDGPPS
510 520 530 540 550
PTIQVKTQQG VPAQPADFQA NAESDTRIQL SWLLPPQERI VKYELVYWAA
560 570 580 590 600
EDEGQQHKVT FDPTSSYTLE DLKPDTLYHF QLAARSDLGV GVFTPTVEAR
610 620 630 640 650
TAQSTPSAPP QKVTCVSTGS TTVRVSWVPP PADSRNGIIT QYSVAYEAVD
660 670 680 690 700
GEDRKRHVVD GISREHSSWD LLGLEKWTEY RVWVRAHTDV GPGPESSPVL
710 720 730 740 750
VRTDEDVPSG PPRKVEVEPL NSTAVHVSWK LPVPNKQHGQ IRGYQVTYVR
760 770 780 790 800
LENGEPRGQP IIQDVMLAEA QETTISGLTP ETTYSITVAA YTTKGDGARS
810 820 830 840 850
KPKVVTTTGA VPGRPTMMVS TTAMHTALLQ WHPPKELPGE LLGYRLQYRR
860 870 880 890 900
ADEARPNTID FGKDDQHFTV TGLHKGATYV FRLAAKNRAG PGEEFEKEIT
910 920 930 940 950
TPEDVPSGFP QNLRVTGLTT STTELTWDPP VLAERNGHIT NYTVVYRDIN
960 970 980 990 1000
SQLELQNVTN DTHLTLLGLK PDTTYDIKVR AHTSKGAGPL SPSIQSRTMP
1010 1020 1030 1040 1050
VEQVFAKNFR VAAAMKTSVL LSWEVPDSYK SAVPFKILYN GQSVEVDGHS
1060 1070 1080 1090 1100
MRKLIADLQP NTEYSFVLMN RGSSAGGLQH LVSIRTAPDL LPQKPLPASA
1110 1120 1130 1140 1150
FIEDGRFSLS MPQVQDPSLV RWFYIVVVPI DRVGGNLLAP RWNTPEELEL
1160 1170 1180 1190 1200
DELLEAIEQG EEKQRRRRRQ AERLKPYVAA QVDVLPDTFT LGDKKSYRGF
1210 1220 1230 1240 1250
YNRPLSPDLS YQCFVLASLK EPMDQKRYAS SPYSDEIVVQ VTPAQQQEEP
1260 1270 1280 1290 1300
EMLWVTGPVL AVILIILIVI AILLFKRKRT HSPSSKDEQS IGLKDSLLAH
1310 1320 1330 1340 1350
SSDPVEMRRL NYQTPGMRDH PPIPITDLAD NIERLKANDG LKFSQEYESI
1360 1370 1380 1390 1400
DPGQQFTWEN SNSEVNKPKN RYANVIAYDH SRVLLTSIDG VPGSDYINAN
1410 1420 1430 1440 1450
YIDGYRKQNA YIATQGPLPE TMGDFWRMVW EQRTATVVMM TRLEEKSRVK
1460 1470 1480 1490 1500
CDQYWPVRGT ETYGLIQVTL VDTVELATYT MRTFALHKSG SSEKRELRQF
1510 1520 1530 1540 1550
QFMAWPDHGV PEYPTPILAF LRRVKACNPL DAGPMVVHCS AGVGRTGCFI
1560 1570 1580 1590 1600
VIDAMLERMK HEKTVDIYGH VTCMRSQRNY MVQTEDQYVF IHEALLEAAM
1610 1620 1630 1640 1650
CGHTEVLARN LYAHIQKLGQ VPPGESVTAM ELEFKLLANS KAHTSRFVSA
1660 1670 1680 1690 1700
NLPCNKFKNR LVNIMPYELT RVCLQPIRGV EGSDYINASF LDGYRQQKAY
1710 1720 1730 1740 1750
IATQGPLAES TEDFWRMLWE HNSTIIVMLT KLREMGREKC HQYWPAERSA
1760 1770 1780 1790 1800
RYQYFVVDPM AEYNMPQYIL REFKVTDARD GQSRTIRQFQ FTDWPEQGVP
1810 1820 1830 1840 1850
KTGEGFIDFI GQVHKTKEQF GQDGPITVHC SAGVGRTGVF ITLSIVLERM
1860 1870 1880 1890
RYEGVVDMFQ TVKTLRTQRP AMVQTEDQYQ LCYRAALEYL GSFDHYAT
Length:1,898
Mass (Da):211,489
Last modified:February 20, 2007 - v1
Checksum:i903FF6814F0BC914
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47G → E in AAG40194 (PubMed:11241288).Curated1
Sequence conflicti482I → V in AAG40194 (PubMed:11241288).Curated1
Sequence conflicti514 – 515QP → RS in AAG40194 (PubMed:11241288).Curated2
Sequence conflicti579H → R in AAG40194 (PubMed:11241288).Curated1
Sequence conflicti1006A → T in AAG40194 (PubMed:11241288).Curated1
Sequence conflicti1184V → A in AAH57166 (PubMed:15489334).Curated1
Sequence conflicti1374N → D in AAG40194 (PubMed:11241288).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300943 mRNA. Translation: AAG40194.1.
AL807774, AL626764 Genomic DNA. Translation: CAM17264.1.
AL626764, AL807774 Genomic DNA. Translation: CAM27382.1.
BC057166 mRNA. Translation: AAH57166.1.
CCDSiCCDS18546.1.
RefSeqiNP_035343.2. NM_011213.2.
UniGeneiMm.29855.

Genome annotation databases

EnsembliENSMUST00000049074; ENSMUSP00000039368; ENSMUSG00000033295.
GeneIDi19268.
KEGGimmu:19268.
UCSCiuc008ujq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300943 mRNA. Translation: AAG40194.1.
AL807774, AL626764 Genomic DNA. Translation: CAM17264.1.
AL626764, AL807774 Genomic DNA. Translation: CAM27382.1.
BC057166 mRNA. Translation: AAH57166.1.
CCDSiCCDS18546.1.
RefSeqiNP_035343.2. NM_011213.2.
UniGeneiMm.29855.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PXHX-ray2.00A30-226[»]
ProteinModelPortaliA2A8L5.
SMRiA2A8L5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202497. 4 interactors.
IntActiA2A8L5. 1 interactor.
STRINGi10090.ENSMUSP00000039368.

PTM databases

iPTMnetiA2A8L5.
PhosphoSitePlusiA2A8L5.
SwissPalmiA2A8L5.

Proteomic databases

MaxQBiA2A8L5.
PaxDbiA2A8L5.
PeptideAtlasiA2A8L5.
PRIDEiA2A8L5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049074; ENSMUSP00000039368; ENSMUSG00000033295.
GeneIDi19268.
KEGGimmu:19268.
UCSCiuc008ujq.1. mouse.

Organism-specific databases

CTDi5792.
MGIiMGI:102695. Ptprf.

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000010250.
InParanoidiA2A8L5.
KOiK05695.
OMAiPSAWHKH.
OrthoDBiEOG091G11WG.
PhylomeDBiA2A8L5.
TreeFamiTF312900.

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapses.

Miscellaneous databases

PROiA2A8L5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033295.
ExpressionAtlasiA2A8L5. baseline and differential.
GenevisibleiA2A8L5. MM.

Family and domain databases

CDDicd00063. FN3. 8 hits.
Gene3Di2.60.40.10. 11 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 8 hits.
SM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
SSF49265. SSF49265. 5 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRF_MOUSE
AccessioniPrimary (citable) accession number: A2A8L5
Secondary accession number(s): Q6PG86, Q9EQ17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.