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A2A8L5

- PTPRF_MOUSE

UniProt

A2A8L5 - PTPRF_MOUSE

Protein

Receptor-type tyrosine-protein phosphatase F

Gene

Ptprf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1507 – 15071SubstrateBy similarity
    Active sitei1539 – 15391Phosphocysteine intermediateBy similarity
    Binding sitei1583 – 15831SubstrateBy similarity
    Active sitei1830 – 18301Phosphocysteine intermediateBy similarity

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB-KW
    2. protein complex binding Source: MGI
    3. protein tyrosine phosphatase activity Source: UniProtKB

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. cell migration Source: UniProtKB
    3. negative regulation of receptor binding Source: UniProtKB
    4. peptidyl-tyrosine dephosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase, Receptor

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Receptor-type tyrosine-protein phosphatase F (EC:3.1.3.48)
    Alternative name(s):
    Leukocyte common antigen related
    Short name:
    LAR
    Gene namesi
    Name:Ptprf
    Synonyms:Lar
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:102695. Ptprf.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 18981869Receptor-type tyrosine-protein phosphatase FPRO_0000370193Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi54 ↔ 1071 PublicationPROSITE-ProRule annotation
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi156 ↔ 2071 PublicationPROSITE-ProRule annotation
    Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi253 ↔ 298PROSITE-ProRule annotation
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi721 – 7211N-linked (GlcNAc...)1 Publication
    Glycosylationi936 – 9361N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi941 – 9411N-linked (GlcNAc...)1 Publication
    Glycosylationi957 – 9571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi960 – 9601N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiA2A8L5.
    PaxDbiA2A8L5.
    PRIDEiA2A8L5.

    PTM databases

    PhosphoSiteiA2A8L5.

    Expressioni

    Tissue specificityi

    Expressed in the cell of the T lineage but not in cells of any other hemopoietic lineage.1 Publication

    Gene expression databases

    ArrayExpressiA2A8L5.
    BgeeiA2A8L5.
    GenevestigatoriA2A8L5.

    Interactioni

    Subunit structurei

    Interacts with GRIP1. Interacts with PPFIA1, PPFIA2 and PPFIA3. Interacts with PTPRF.By similarity

    Protein-protein interaction databases

    BioGridi202497. 2 interactions.
    IntActiA2A8L5. 1 interaction.

    Structurei

    Secondary structure

    1
    1898
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi31 – 377
    Beta strandi42 – 454
    Beta strandi50 – 6011
    Beta strandi63 – 686
    Beta strandi78 – 836
    Helixi84 – 863
    Beta strandi88 – 936
    Helixi98 – 1014
    Beta strandi103 – 1108
    Beta strandi115 – 12511
    Beta strandi136 – 1394
    Beta strandi144 – 1463
    Beta strandi148 – 1503
    Beta strandi152 – 1543
    Beta strandi157 – 1593
    Beta strandi165 – 1706
    Turni177 – 1793
    Beta strandi180 – 1867
    Beta strandi192 – 1943
    Helixi199 – 2013
    Beta strandi203 – 2119
    Beta strandi214 – 2174
    Beta strandi221 – 2255

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3PXHX-ray2.00A30-226[»]
    ProteinModelPortaliA2A8L5.
    SMRiA2A8L5. Positions 30-1001, 1325-1893.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 12541225ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1276 – 1898623CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1255 – 127521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 12391Ig-like C2-type 1Add
    BLAST
    Domaini135 – 22490Ig-like C2-type 2Add
    BLAST
    Domaini232 – 31483Ig-like C2-type 3Add
    BLAST
    Domaini321 – 41191Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini416 – 51095Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini514 – 60491Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini609 – 70698Fibronectin type-III 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini711 – 810100Fibronectin type-III 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini811 – 90494Fibronectin type-III 6PROSITE-ProRule annotationAdd
    BLAST
    Domaini909 – 100193Fibronectin type-III 7PROSITE-ProRule annotationAdd
    BLAST
    Domaini1005 – 108985Fibronectin type-III 8PROSITE-ProRule annotationAdd
    BLAST
    Domaini1343 – 1598256Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1630 – 1889260Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni68 – 7710Heparin-binding
    Regioni1539 – 15457Substrate bindingBy similarity

    Domaini

    The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.By similarity

    Sequence similaritiesi

    Contains 8 fibronectin type-III domains.PROSITE-ProRule annotation
    Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00590000082937.
    HOGENOMiHOG000010250.
    InParanoidiA2A8L5.
    KOiK05695.
    OMAiVFTPTIE.
    OrthoDBiEOG7M98FB.
    PhylomeDBiA2A8L5.
    TreeFamiTF312900.

    Family and domain databases

    Gene3Di2.60.40.10. 10 hits.
    3.90.190.10. 2 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00041. fn3. 7 hits.
    PF07679. I-set. 3 hits.
    PF00102. Y_phosphatase. 2 hits.
    [Graphical view]
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00060. FN3. 8 hits.
    SM00409. IG. 1 hit.
    SM00408. IGc2. 2 hits.
    SM00194. PTPc. 2 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 5 hits.
    SSF52799. SSF52799. 2 hits.
    PROSITEiPS50853. FN3. 8 hits.
    PS50835. IG_LIKE. 3 hits.
    PS00383. TYR_PHOSPHATASE_1. 2 hits.
    PS50056. TYR_PHOSPHATASE_2. 2 hits.
    PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A2A8L5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPEPAPGRR MVPLVPALVM LGLMAGAHGD SKPVFVKVPE DQTGLSGGVA     50
    SFVCQATGEP KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR 100
    DEAIYECTAT NSLGEINTSA KLSVLEEDQL PSGFPTIDMG PQLKVVEKGR 150
    TATMLCAAGG NPDPEISWFK DFLPVDPAAS NGRIKQLRSG ALQIESSEES 200
    DQGKYECVAT NSAGTRYSAP ANLYVRVRRV APRFSIPPSS QEVMPGGSVN 250
    LTCVAVGAPM PYVKWMMGAE ELTKEDEMPV GRNVLELSNV MRSANYTCVA 300
    ISSLGMIEAT AQVTVKALPK PPIDLVVTET TATSVTLTWD SGNTEPVSFY 350
    GIQYRAAGTD GPFQEVDGVA STRYSIGGLS PFSEYAFRVL AVNSIGRGPP 400
    SEAVRARTGE QAPSSPPRRV QARMLSASTM LVQWEPPEEP NGLVRGYRVY 450
    YTPDSRRPLS AWHKHNTDAG LLTTVGSLLP GITYSLRVLA FTAVGDGPPS 500
    PTIQVKTQQG VPAQPADFQA NAESDTRIQL SWLLPPQERI VKYELVYWAA 550
    EDEGQQHKVT FDPTSSYTLE DLKPDTLYHF QLAARSDLGV GVFTPTVEAR 600
    TAQSTPSAPP QKVTCVSTGS TTVRVSWVPP PADSRNGIIT QYSVAYEAVD 650
    GEDRKRHVVD GISREHSSWD LLGLEKWTEY RVWVRAHTDV GPGPESSPVL 700
    VRTDEDVPSG PPRKVEVEPL NSTAVHVSWK LPVPNKQHGQ IRGYQVTYVR 750
    LENGEPRGQP IIQDVMLAEA QETTISGLTP ETTYSITVAA YTTKGDGARS 800
    KPKVVTTTGA VPGRPTMMVS TTAMHTALLQ WHPPKELPGE LLGYRLQYRR 850
    ADEARPNTID FGKDDQHFTV TGLHKGATYV FRLAAKNRAG PGEEFEKEIT 900
    TPEDVPSGFP QNLRVTGLTT STTELTWDPP VLAERNGHIT NYTVVYRDIN 950
    SQLELQNVTN DTHLTLLGLK PDTTYDIKVR AHTSKGAGPL SPSIQSRTMP 1000
    VEQVFAKNFR VAAAMKTSVL LSWEVPDSYK SAVPFKILYN GQSVEVDGHS 1050
    MRKLIADLQP NTEYSFVLMN RGSSAGGLQH LVSIRTAPDL LPQKPLPASA 1100
    FIEDGRFSLS MPQVQDPSLV RWFYIVVVPI DRVGGNLLAP RWNTPEELEL 1150
    DELLEAIEQG EEKQRRRRRQ AERLKPYVAA QVDVLPDTFT LGDKKSYRGF 1200
    YNRPLSPDLS YQCFVLASLK EPMDQKRYAS SPYSDEIVVQ VTPAQQQEEP 1250
    EMLWVTGPVL AVILIILIVI AILLFKRKRT HSPSSKDEQS IGLKDSLLAH 1300
    SSDPVEMRRL NYQTPGMRDH PPIPITDLAD NIERLKANDG LKFSQEYESI 1350
    DPGQQFTWEN SNSEVNKPKN RYANVIAYDH SRVLLTSIDG VPGSDYINAN 1400
    YIDGYRKQNA YIATQGPLPE TMGDFWRMVW EQRTATVVMM TRLEEKSRVK 1450
    CDQYWPVRGT ETYGLIQVTL VDTVELATYT MRTFALHKSG SSEKRELRQF 1500
    QFMAWPDHGV PEYPTPILAF LRRVKACNPL DAGPMVVHCS AGVGRTGCFI 1550
    VIDAMLERMK HEKTVDIYGH VTCMRSQRNY MVQTEDQYVF IHEALLEAAM 1600
    CGHTEVLARN LYAHIQKLGQ VPPGESVTAM ELEFKLLANS KAHTSRFVSA 1650
    NLPCNKFKNR LVNIMPYELT RVCLQPIRGV EGSDYINASF LDGYRQQKAY 1700
    IATQGPLAES TEDFWRMLWE HNSTIIVMLT KLREMGREKC HQYWPAERSA 1750
    RYQYFVVDPM AEYNMPQYIL REFKVTDARD GQSRTIRQFQ FTDWPEQGVP 1800
    KTGEGFIDFI GQVHKTKEQF GQDGPITVHC SAGVGRTGVF ITLSIVLERM 1850
    RYEGVVDMFQ TVKTLRTQRP AMVQTEDQYQ LCYRAALEYL GSFDHYAT 1898
    Length:1,898
    Mass (Da):211,489
    Last modified:February 20, 2007 - v1
    Checksum:i903FF6814F0BC914
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471G → E in AAG40194. (PubMed:11241288)Curated
    Sequence conflicti482 – 4821I → V in AAG40194. (PubMed:11241288)Curated
    Sequence conflicti514 – 5152QP → RS in AAG40194. (PubMed:11241288)Curated
    Sequence conflicti579 – 5791H → R in AAG40194. (PubMed:11241288)Curated
    Sequence conflicti1006 – 10061A → T in AAG40194. (PubMed:11241288)Curated
    Sequence conflicti1184 – 11841V → A in AAH57166. (PubMed:15489334)Curated
    Sequence conflicti1374 – 13741N → D in AAG40194. (PubMed:11241288)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF300943 mRNA. Translation: AAG40194.1.
    AL807774, AL626764 Genomic DNA. Translation: CAM17264.1.
    AL626764, AL807774 Genomic DNA. Translation: CAM27382.1.
    BC057166 mRNA. Translation: AAH57166.1.
    CCDSiCCDS18546.1.
    RefSeqiNP_035343.2. NM_011213.2.
    UniGeneiMm.29855.

    Genome annotation databases

    EnsembliENSMUST00000049074; ENSMUSP00000039368; ENSMUSG00000033295.
    GeneIDi19268.
    KEGGimmu:19268.
    UCSCiuc008ujq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF300943 mRNA. Translation: AAG40194.1 .
    AL807774 , AL626764 Genomic DNA. Translation: CAM17264.1 .
    AL626764 , AL807774 Genomic DNA. Translation: CAM27382.1 .
    BC057166 mRNA. Translation: AAH57166.1 .
    CCDSi CCDS18546.1.
    RefSeqi NP_035343.2. NM_011213.2.
    UniGenei Mm.29855.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3PXH X-ray 2.00 A 30-226 [» ]
    ProteinModelPortali A2A8L5.
    SMRi A2A8L5. Positions 30-1001, 1325-1893.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202497. 2 interactions.
    IntActi A2A8L5. 1 interaction.

    PTM databases

    PhosphoSitei A2A8L5.

    Proteomic databases

    MaxQBi A2A8L5.
    PaxDbi A2A8L5.
    PRIDEi A2A8L5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049074 ; ENSMUSP00000039368 ; ENSMUSG00000033295 .
    GeneIDi 19268.
    KEGGi mmu:19268.
    UCSCi uc008ujq.1. mouse.

    Organism-specific databases

    CTDi 5792.
    MGIi MGI:102695. Ptprf.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00590000082937.
    HOGENOMi HOG000010250.
    InParanoidi A2A8L5.
    KOi K05695.
    OMAi VFTPTIE.
    OrthoDBi EOG7M98FB.
    PhylomeDBi A2A8L5.
    TreeFami TF312900.

    Miscellaneous databases

    NextBioi 296150.
    PROi A2A8L5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A2A8L5.
    Bgeei A2A8L5.
    Genevestigatori A2A8L5.

    Family and domain databases

    Gene3Di 2.60.40.10. 10 hits.
    3.90.190.10. 2 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00041. fn3. 7 hits.
    PF07679. I-set. 3 hits.
    PF00102. Y_phosphatase. 2 hits.
    [Graphical view ]
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00060. FN3. 8 hits.
    SM00409. IG. 1 hit.
    SM00408. IGc2. 2 hits.
    SM00194. PTPc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 5 hits.
    SSF52799. SSF52799. 2 hits.
    PROSITEi PS50853. FN3. 8 hits.
    PS50835. IG_LIKE. 3 hits.
    PS00383. TYR_PHOSPHATASE_1. 2 hits.
    PS50056. TYR_PHOSPHATASE_2. 2 hits.
    PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Within the hemopoietic system, LAR phosphatase is a T cell lineage-specific adhesion receptor-like protein whose phosphatase activity appears dispensable for T cell development, repertoire selection and function."
      Terszowski G., Jankowski A., Hendriks W.J.A.J., Rolink A.G., Kisielow P.
      Eur. J. Immunol. 31:832-840(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Thymus.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 370-1898.
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-721; ASN-936; ASN-941 AND ASN-960.
    5. "The immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR adopt an unusual horseshoe-like conformation."
      Biersmith B.H., Hammel M., Geisbrecht E.R., Bouyain S.
      J. Mol. Biol. 408:616-627(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 30-226, HEPARIN-BINDING REGION, DISULFIDE BONDS.

    Entry informationi

    Entry nameiPTPRF_MOUSE
    AccessioniPrimary (citable) accession number: A2A8L5
    Secondary accession number(s): Q6PG86, Q9EQ17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3