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A2A8L5

- PTPRF_MOUSE

UniProt

A2A8L5 - PTPRF_MOUSE

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Protein

Receptor-type tyrosine-protein phosphatase F

Gene
Ptprf, Lar
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity By similarity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1507 – 15071Substrate By similarity
Active sitei1539 – 15391Phosphocysteine intermediate By similarity
Binding sitei1583 – 15831Substrate By similarity
Active sitei1830 – 18301Phosphocysteine intermediate By similarity

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW
  2. protein complex binding Source: MGI
  3. protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. cell migration Source: UniProtKB
  3. negative regulation of receptor binding Source: UniProtKB
  4. peptidyl-tyrosine dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase F (EC:3.1.3.48)
Alternative name(s):
Leukocyte common antigen related
Short name:
LAR
Gene namesi
Name:Ptprf
Synonyms:Lar
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:102695. Ptprf.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 12541225Extracellular Reviewed predictionAdd
BLAST
Transmembranei1255 – 127521Helical; Reviewed predictionAdd
BLAST
Topological domaini1276 – 1898623Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed predictionAdd
BLAST
Chaini30 – 18981869Receptor-type tyrosine-protein phosphatase FPRO_0000370193Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 1071 Publication
Glycosylationi117 – 1171N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi156 ↔ 2071 Publication
Glycosylationi250 – 2501N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi253 ↔ 298 By similarity
Glycosylationi295 – 2951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi721 – 7211N-linked (GlcNAc...)1 Publication
Glycosylationi936 – 9361N-linked (GlcNAc...); atypical1 Publication
Glycosylationi941 – 9411N-linked (GlcNAc...)1 Publication
Glycosylationi957 – 9571N-linked (GlcNAc...) Reviewed prediction
Glycosylationi960 – 9601N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiA2A8L5.
PaxDbiA2A8L5.
PRIDEiA2A8L5.

PTM databases

PhosphoSiteiA2A8L5.

Expressioni

Tissue specificityi

Expressed in the cell of the T lineage but not in cells of any other hemopoietic lineage.1 Publication

Gene expression databases

ArrayExpressiA2A8L5.
BgeeiA2A8L5.
GenevestigatoriA2A8L5.

Interactioni

Subunit structurei

Interacts with GRIP1 By similarity. Interacts with PPFIA1, PPFIA2 and PPFIA3 By similarity. Interacts with PTPRF By similarity.

Protein-protein interaction databases

BioGridi202497. 2 interactions.
IntActiA2A8L5. 1 interaction.

Structurei

Secondary structure

1
1898
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi31 – 377
Beta strandi42 – 454
Beta strandi50 – 6011
Beta strandi63 – 686
Beta strandi78 – 836
Helixi84 – 863
Beta strandi88 – 936
Helixi98 – 1014
Beta strandi103 – 1108
Beta strandi115 – 12511
Beta strandi136 – 1394
Beta strandi144 – 1463
Beta strandi148 – 1503
Beta strandi152 – 1543
Beta strandi157 – 1593
Beta strandi165 – 1706
Turni177 – 1793
Beta strandi180 – 1867
Beta strandi192 – 1943
Helixi199 – 2013
Beta strandi203 – 2119
Beta strandi214 – 2174
Beta strandi221 – 2255

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PXHX-ray2.00A30-226[»]
ProteinModelPortaliA2A8L5.
SMRiA2A8L5. Positions 30-1001, 1325-1893.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 12391Ig-like C2-type 1Add
BLAST
Domaini135 – 22490Ig-like C2-type 2Add
BLAST
Domaini232 – 31483Ig-like C2-type 3Add
BLAST
Domaini321 – 41191Fibronectin type-III 1Add
BLAST
Domaini416 – 51095Fibronectin type-III 2Add
BLAST
Domaini514 – 60491Fibronectin type-III 3Add
BLAST
Domaini609 – 70698Fibronectin type-III 4Add
BLAST
Domaini711 – 810100Fibronectin type-III 5Add
BLAST
Domaini811 – 90494Fibronectin type-III 6Add
BLAST
Domaini909 – 100193Fibronectin type-III 7Add
BLAST
Domaini1005 – 108985Fibronectin type-III 8Add
BLAST
Domaini1343 – 1598256Tyrosine-protein phosphatase 1Add
BLAST
Domaini1630 – 1889260Tyrosine-protein phosphatase 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 7710Heparin-binding
Regioni1539 – 15457Substrate binding By similarity

Domaini

The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one By similarity.

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00590000082937.
HOGENOMiHOG000010250.
InParanoidiA2A8L5.
KOiK05695.
OMAiVFTPTIE.
OrthoDBiEOG7M98FB.
PhylomeDBiA2A8L5.
TreeFamiTF312900.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 8 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 5 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2A8L5-1 [UniParc]FASTAAdd to Basket

« Hide

MAPEPAPGRR MVPLVPALVM LGLMAGAHGD SKPVFVKVPE DQTGLSGGVA     50
SFVCQATGEP KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR 100
DEAIYECTAT NSLGEINTSA KLSVLEEDQL PSGFPTIDMG PQLKVVEKGR 150
TATMLCAAGG NPDPEISWFK DFLPVDPAAS NGRIKQLRSG ALQIESSEES 200
DQGKYECVAT NSAGTRYSAP ANLYVRVRRV APRFSIPPSS QEVMPGGSVN 250
LTCVAVGAPM PYVKWMMGAE ELTKEDEMPV GRNVLELSNV MRSANYTCVA 300
ISSLGMIEAT AQVTVKALPK PPIDLVVTET TATSVTLTWD SGNTEPVSFY 350
GIQYRAAGTD GPFQEVDGVA STRYSIGGLS PFSEYAFRVL AVNSIGRGPP 400
SEAVRARTGE QAPSSPPRRV QARMLSASTM LVQWEPPEEP NGLVRGYRVY 450
YTPDSRRPLS AWHKHNTDAG LLTTVGSLLP GITYSLRVLA FTAVGDGPPS 500
PTIQVKTQQG VPAQPADFQA NAESDTRIQL SWLLPPQERI VKYELVYWAA 550
EDEGQQHKVT FDPTSSYTLE DLKPDTLYHF QLAARSDLGV GVFTPTVEAR 600
TAQSTPSAPP QKVTCVSTGS TTVRVSWVPP PADSRNGIIT QYSVAYEAVD 650
GEDRKRHVVD GISREHSSWD LLGLEKWTEY RVWVRAHTDV GPGPESSPVL 700
VRTDEDVPSG PPRKVEVEPL NSTAVHVSWK LPVPNKQHGQ IRGYQVTYVR 750
LENGEPRGQP IIQDVMLAEA QETTISGLTP ETTYSITVAA YTTKGDGARS 800
KPKVVTTTGA VPGRPTMMVS TTAMHTALLQ WHPPKELPGE LLGYRLQYRR 850
ADEARPNTID FGKDDQHFTV TGLHKGATYV FRLAAKNRAG PGEEFEKEIT 900
TPEDVPSGFP QNLRVTGLTT STTELTWDPP VLAERNGHIT NYTVVYRDIN 950
SQLELQNVTN DTHLTLLGLK PDTTYDIKVR AHTSKGAGPL SPSIQSRTMP 1000
VEQVFAKNFR VAAAMKTSVL LSWEVPDSYK SAVPFKILYN GQSVEVDGHS 1050
MRKLIADLQP NTEYSFVLMN RGSSAGGLQH LVSIRTAPDL LPQKPLPASA 1100
FIEDGRFSLS MPQVQDPSLV RWFYIVVVPI DRVGGNLLAP RWNTPEELEL 1150
DELLEAIEQG EEKQRRRRRQ AERLKPYVAA QVDVLPDTFT LGDKKSYRGF 1200
YNRPLSPDLS YQCFVLASLK EPMDQKRYAS SPYSDEIVVQ VTPAQQQEEP 1250
EMLWVTGPVL AVILIILIVI AILLFKRKRT HSPSSKDEQS IGLKDSLLAH 1300
SSDPVEMRRL NYQTPGMRDH PPIPITDLAD NIERLKANDG LKFSQEYESI 1350
DPGQQFTWEN SNSEVNKPKN RYANVIAYDH SRVLLTSIDG VPGSDYINAN 1400
YIDGYRKQNA YIATQGPLPE TMGDFWRMVW EQRTATVVMM TRLEEKSRVK 1450
CDQYWPVRGT ETYGLIQVTL VDTVELATYT MRTFALHKSG SSEKRELRQF 1500
QFMAWPDHGV PEYPTPILAF LRRVKACNPL DAGPMVVHCS AGVGRTGCFI 1550
VIDAMLERMK HEKTVDIYGH VTCMRSQRNY MVQTEDQYVF IHEALLEAAM 1600
CGHTEVLARN LYAHIQKLGQ VPPGESVTAM ELEFKLLANS KAHTSRFVSA 1650
NLPCNKFKNR LVNIMPYELT RVCLQPIRGV EGSDYINASF LDGYRQQKAY 1700
IATQGPLAES TEDFWRMLWE HNSTIIVMLT KLREMGREKC HQYWPAERSA 1750
RYQYFVVDPM AEYNMPQYIL REFKVTDARD GQSRTIRQFQ FTDWPEQGVP 1800
KTGEGFIDFI GQVHKTKEQF GQDGPITVHC SAGVGRTGVF ITLSIVLERM 1850
RYEGVVDMFQ TVKTLRTQRP AMVQTEDQYQ LCYRAALEYL GSFDHYAT 1898
Length:1,898
Mass (Da):211,489
Last modified:February 20, 2007 - v1
Checksum:i903FF6814F0BC914
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471G → E in AAG40194. 1 Publication
Sequence conflicti482 – 4821I → V in AAG40194. 1 Publication
Sequence conflicti514 – 5152QP → RS in AAG40194. 1 Publication
Sequence conflicti579 – 5791H → R in AAG40194. 1 Publication
Sequence conflicti1006 – 10061A → T in AAG40194. 1 Publication
Sequence conflicti1184 – 11841V → A in AAH57166. 1 Publication
Sequence conflicti1374 – 13741N → D in AAG40194. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF300943 mRNA. Translation: AAG40194.1.
AL807774, AL626764 Genomic DNA. Translation: CAM17264.1.
AL626764, AL807774 Genomic DNA. Translation: CAM27382.1.
BC057166 mRNA. Translation: AAH57166.1.
CCDSiCCDS18546.1.
RefSeqiNP_035343.2. NM_011213.2.
UniGeneiMm.29855.

Genome annotation databases

EnsembliENSMUST00000049074; ENSMUSP00000039368; ENSMUSG00000033295.
GeneIDi19268.
KEGGimmu:19268.
UCSCiuc008ujq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF300943 mRNA. Translation: AAG40194.1 .
AL807774 , AL626764 Genomic DNA. Translation: CAM17264.1 .
AL626764 , AL807774 Genomic DNA. Translation: CAM27382.1 .
BC057166 mRNA. Translation: AAH57166.1 .
CCDSi CCDS18546.1.
RefSeqi NP_035343.2. NM_011213.2.
UniGenei Mm.29855.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3PXH X-ray 2.00 A 30-226 [» ]
ProteinModelPortali A2A8L5.
SMRi A2A8L5. Positions 30-1001, 1325-1893.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202497. 2 interactions.
IntActi A2A8L5. 1 interaction.

PTM databases

PhosphoSitei A2A8L5.

Proteomic databases

MaxQBi A2A8L5.
PaxDbi A2A8L5.
PRIDEi A2A8L5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000049074 ; ENSMUSP00000039368 ; ENSMUSG00000033295 .
GeneIDi 19268.
KEGGi mmu:19268.
UCSCi uc008ujq.1. mouse.

Organism-specific databases

CTDi 5792.
MGIi MGI:102695. Ptprf.

Phylogenomic databases

eggNOGi COG5599.
GeneTreei ENSGT00590000082937.
HOGENOMi HOG000010250.
InParanoidi A2A8L5.
KOi K05695.
OMAi VFTPTIE.
OrthoDBi EOG7M98FB.
PhylomeDBi A2A8L5.
TreeFami TF312900.

Miscellaneous databases

NextBioi 296150.
PROi A2A8L5.
SOURCEi Search...

Gene expression databases

ArrayExpressi A2A8L5.
Bgeei A2A8L5.
Genevestigatori A2A8L5.

Family and domain databases

Gene3Di 2.60.40.10. 10 hits.
3.90.190.10. 2 hits.
InterProi IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view ]
Pfami PF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00700. PRTYPHPHTASE.
SMARTi SM00060. FN3. 8 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
SM00194. PTPc. 2 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 5 hits.
SSF52799. SSF52799. 2 hits.
PROSITEi PS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Within the hemopoietic system, LAR phosphatase is a T cell lineage-specific adhesion receptor-like protein whose phosphatase activity appears dispensable for T cell development, repertoire selection and function."
    Terszowski G., Jankowski A., Hendriks W.J.A.J., Rolink A.G., Kisielow P.
    Eur. J. Immunol. 31:832-840(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 370-1898.
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-721; ASN-936; ASN-941 AND ASN-960.
  5. "The immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR adopt an unusual horseshoe-like conformation."
    Biersmith B.H., Hammel M., Geisbrecht E.R., Bouyain S.
    J. Mol. Biol. 408:616-627(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 30-226, HEPARIN-BINDING REGION, DISULFIDE BONDS.

Entry informationi

Entry nameiPTPRF_MOUSE
AccessioniPrimary (citable) accession number: A2A8L5
Secondary accession number(s): Q6PG86, Q9EQ17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 20, 2007
Last modified: September 3, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi