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A2A8L5 (PTPRF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase F

EC=3.1.3.48
Alternative name(s):
Leukocyte common antigen related
Short name=LAR
Gene names
Name:Ptprf
Synonyms:Lar
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1898 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with GRIP1 By similarity. Interacts with PPFIA1, PPFIA2 and PPFIA3 By similarity. Interacts with PTPRF By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Expressed in the cell of the T lineage but not in cells of any other hemopoietic lineage. Ref.1

Domain

The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one By similarity.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.

Contains 8 fibronectin type-III domains.

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 tyrosine-protein phosphatase domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 18981869Receptor-type tyrosine-protein phosphatase F
PRO_0000370193

Regions

Topological domain30 – 12541225Extracellular Potential
Transmembrane1255 – 127521Helical; Potential
Topological domain1276 – 1898623Cytoplasmic Potential
Domain33 – 12391Ig-like C2-type 1
Domain135 – 22490Ig-like C2-type 2
Domain232 – 31483Ig-like C2-type 3
Domain321 – 41191Fibronectin type-III 1
Domain416 – 51095Fibronectin type-III 2
Domain514 – 60491Fibronectin type-III 3
Domain609 – 70698Fibronectin type-III 4
Domain711 – 810100Fibronectin type-III 5
Domain811 – 90494Fibronectin type-III 6
Domain909 – 100193Fibronectin type-III 7
Domain1005 – 108985Fibronectin type-III 8
Domain1343 – 1598256Tyrosine-protein phosphatase 1
Domain1630 – 1889260Tyrosine-protein phosphatase 2
Region68 – 7710Heparin-binding
Region1539 – 15457Substrate binding By similarity

Sites

Active site15391Phosphocysteine intermediate By similarity
Active site18301Phosphocysteine intermediate By similarity
Binding site15071Substrate By similarity
Binding site15831Substrate By similarity

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation2501N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation7211N-linked (GlcNAc...) Ref.4
Glycosylation9361N-linked (GlcNAc...); atypical Ref.4
Glycosylation9411N-linked (GlcNAc...) Ref.4
Glycosylation9571N-linked (GlcNAc...) Potential
Glycosylation9601N-linked (GlcNAc...) Ref.4
Disulfide bond54 ↔ 107 Ref.5
Disulfide bond156 ↔ 207 Ref.5
Disulfide bond253 ↔ 298 By similarity

Experimental info

Sequence conflict471G → E in AAG40194. Ref.1
Sequence conflict4821I → V in AAG40194. Ref.1
Sequence conflict514 – 5152QP → RS in AAG40194. Ref.1
Sequence conflict5791H → R in AAG40194. Ref.1
Sequence conflict10061A → T in AAG40194. Ref.1
Sequence conflict11841V → A in AAH57166. Ref.3
Sequence conflict13741N → D in AAG40194. Ref.1

Secondary structure

............................................. 1898
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
A2A8L5 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 903FF6814F0BC914

FASTA1,898211,489
        10         20         30         40         50         60 
MAPEPAPGRR MVPLVPALVM LGLMAGAHGD SKPVFVKVPE DQTGLSGGVA SFVCQATGEP 

        70         80         90        100        110        120 
KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR DEAIYECTAT NSLGEINTSA 

       130        140        150        160        170        180 
KLSVLEEDQL PSGFPTIDMG PQLKVVEKGR TATMLCAAGG NPDPEISWFK DFLPVDPAAS 

       190        200        210        220        230        240 
NGRIKQLRSG ALQIESSEES DQGKYECVAT NSAGTRYSAP ANLYVRVRRV APRFSIPPSS 

       250        260        270        280        290        300 
QEVMPGGSVN LTCVAVGAPM PYVKWMMGAE ELTKEDEMPV GRNVLELSNV MRSANYTCVA 

       310        320        330        340        350        360 
ISSLGMIEAT AQVTVKALPK PPIDLVVTET TATSVTLTWD SGNTEPVSFY GIQYRAAGTD 

       370        380        390        400        410        420 
GPFQEVDGVA STRYSIGGLS PFSEYAFRVL AVNSIGRGPP SEAVRARTGE QAPSSPPRRV 

       430        440        450        460        470        480 
QARMLSASTM LVQWEPPEEP NGLVRGYRVY YTPDSRRPLS AWHKHNTDAG LLTTVGSLLP 

       490        500        510        520        530        540 
GITYSLRVLA FTAVGDGPPS PTIQVKTQQG VPAQPADFQA NAESDTRIQL SWLLPPQERI 

       550        560        570        580        590        600 
VKYELVYWAA EDEGQQHKVT FDPTSSYTLE DLKPDTLYHF QLAARSDLGV GVFTPTVEAR 

       610        620        630        640        650        660 
TAQSTPSAPP QKVTCVSTGS TTVRVSWVPP PADSRNGIIT QYSVAYEAVD GEDRKRHVVD 

       670        680        690        700        710        720 
GISREHSSWD LLGLEKWTEY RVWVRAHTDV GPGPESSPVL VRTDEDVPSG PPRKVEVEPL 

       730        740        750        760        770        780 
NSTAVHVSWK LPVPNKQHGQ IRGYQVTYVR LENGEPRGQP IIQDVMLAEA QETTISGLTP 

       790        800        810        820        830        840 
ETTYSITVAA YTTKGDGARS KPKVVTTTGA VPGRPTMMVS TTAMHTALLQ WHPPKELPGE 

       850        860        870        880        890        900 
LLGYRLQYRR ADEARPNTID FGKDDQHFTV TGLHKGATYV FRLAAKNRAG PGEEFEKEIT 

       910        920        930        940        950        960 
TPEDVPSGFP QNLRVTGLTT STTELTWDPP VLAERNGHIT NYTVVYRDIN SQLELQNVTN 

       970        980        990       1000       1010       1020 
DTHLTLLGLK PDTTYDIKVR AHTSKGAGPL SPSIQSRTMP VEQVFAKNFR VAAAMKTSVL 

      1030       1040       1050       1060       1070       1080 
LSWEVPDSYK SAVPFKILYN GQSVEVDGHS MRKLIADLQP NTEYSFVLMN RGSSAGGLQH 

      1090       1100       1110       1120       1130       1140 
LVSIRTAPDL LPQKPLPASA FIEDGRFSLS MPQVQDPSLV RWFYIVVVPI DRVGGNLLAP 

      1150       1160       1170       1180       1190       1200 
RWNTPEELEL DELLEAIEQG EEKQRRRRRQ AERLKPYVAA QVDVLPDTFT LGDKKSYRGF 

      1210       1220       1230       1240       1250       1260 
YNRPLSPDLS YQCFVLASLK EPMDQKRYAS SPYSDEIVVQ VTPAQQQEEP EMLWVTGPVL 

      1270       1280       1290       1300       1310       1320 
AVILIILIVI AILLFKRKRT HSPSSKDEQS IGLKDSLLAH SSDPVEMRRL NYQTPGMRDH 

      1330       1340       1350       1360       1370       1380 
PPIPITDLAD NIERLKANDG LKFSQEYESI DPGQQFTWEN SNSEVNKPKN RYANVIAYDH 

      1390       1400       1410       1420       1430       1440 
SRVLLTSIDG VPGSDYINAN YIDGYRKQNA YIATQGPLPE TMGDFWRMVW EQRTATVVMM 

      1450       1460       1470       1480       1490       1500 
TRLEEKSRVK CDQYWPVRGT ETYGLIQVTL VDTVELATYT MRTFALHKSG SSEKRELRQF 

      1510       1520       1530       1540       1550       1560 
QFMAWPDHGV PEYPTPILAF LRRVKACNPL DAGPMVVHCS AGVGRTGCFI VIDAMLERMK 

      1570       1580       1590       1600       1610       1620 
HEKTVDIYGH VTCMRSQRNY MVQTEDQYVF IHEALLEAAM CGHTEVLARN LYAHIQKLGQ 

      1630       1640       1650       1660       1670       1680 
VPPGESVTAM ELEFKLLANS KAHTSRFVSA NLPCNKFKNR LVNIMPYELT RVCLQPIRGV 

      1690       1700       1710       1720       1730       1740 
EGSDYINASF LDGYRQQKAY IATQGPLAES TEDFWRMLWE HNSTIIVMLT KLREMGREKC 

      1750       1760       1770       1780       1790       1800 
HQYWPAERSA RYQYFVVDPM AEYNMPQYIL REFKVTDARD GQSRTIRQFQ FTDWPEQGVP 

      1810       1820       1830       1840       1850       1860 
KTGEGFIDFI GQVHKTKEQF GQDGPITVHC SAGVGRTGVF ITLSIVLERM RYEGVVDMFQ 

      1870       1880       1890 
TVKTLRTQRP AMVQTEDQYQ LCYRAALEYL GSFDHYAT 

« Hide

References

« Hide 'large scale' references
[1]"Within the hemopoietic system, LAR phosphatase is a T cell lineage-specific adhesion receptor-like protein whose phosphatase activity appears dispensable for T cell development, repertoire selection and function."
Terszowski G., Jankowski A., Hendriks W.J.A.J., Rolink A.G., Kisielow P.
Eur. J. Immunol. 31:832-840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 370-1898.
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-721; ASN-936; ASN-941 AND ASN-960.
[5]"The immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR adopt an unusual horseshoe-like conformation."
Biersmith B.H., Hammel M., Geisbrecht E.R., Bouyain S.
J. Mol. Biol. 408:616-627(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 30-226, HEPARIN-BINDING REGION, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF300943 mRNA. Translation: AAG40194.1.
AL807774, AL626764 Genomic DNA. Translation: CAM17264.1.
AL626764, AL807774 Genomic DNA. Translation: CAM27382.1.
BC057166 mRNA. Translation: AAH57166.1.
RefSeqNP_035343.2. NM_011213.2.
UniGeneMm.29855.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3PXHX-ray2.00A30-226[»]
ProteinModelPortalA2A8L5.
SMRA2A8L5. Positions 30-1001, 1325-1893.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202497. 2 interactions.
IntActA2A8L5. 1 interaction.

PTM databases

PhosphoSiteA2A8L5.

Proteomic databases

PaxDbA2A8L5.
PRIDEA2A8L5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049074; ENSMUSP00000039368; ENSMUSG00000033295.
GeneID19268.
KEGGmmu:19268.
UCSCuc008ujq.1. mouse.

Organism-specific databases

CTD5792.
MGIMGI:102695. Ptprf.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00590000082937.
HOGENOMHOG000010250.
InParanoidA2A8L5.
KOK05695.
OMAVFTPTIE.
OrthoDBEOG7M98FB.
PhylomeDBA2A8L5.
TreeFamTF312900.

Gene expression databases

ArrayExpressA2A8L5.
BgeeA2A8L5.
GenevestigatorA2A8L5.

Family and domain databases

Gene3D2.60.40.10. 10 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamPF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00060. FN3. 8 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 2 hits.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 5 hits.
PROSITEPS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio296150.
PROA2A8L5.
SOURCESearch...

Entry information

Entry namePTPRF_MOUSE
AccessionPrimary (citable) accession number: A2A8L5
Secondary accession number(s): Q6PG86, Q9EQ17
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 20, 2007
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot