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Protein

E3 ubiquitin-protein ligase RNF19B

Gene

Rnf19b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. Protects against staurosporin-induced cell death (By similarity).By similarity

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei299By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri116 – 163RING-type 1PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri183 – 248IBR-typeAdd BLAST66
Zinc fingeri284 – 315RING-type 2PROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • interferon-gamma secretion Source: MGI
  • natural killer cell mediated cytotoxicity Source: MGI
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein polyubiquitination Source: GO_Central

Keywordsi

Molecular functionTransferase
Biological processAdaptive immunity, Immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF19B (EC:2.3.2.31By similarity)
Alternative name(s):
IBR domain-containing protein 3
Natural killer lytic-associated molecule
RING finger protein 19B
Gene namesi
Name:Rnf19b
Synonyms:Ibrdc3, Nklam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922484 Rnf19b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003071881 – 732E3 ubiquitin-protein ligase RNF19BAdd BLAST732

Proteomic databases

PaxDbiA2A7Q9
PRIDEiA2A7Q9

PTM databases

iPTMnetiA2A7Q9
PhosphoSitePlusiA2A7Q9

Expressioni

Tissue specificityi

Expressed specifically in natural killer cells, activated macrophages and cytotoxic T-cells (PubMed:10912506). Present in macrophages (at protein level) (PubMed:10912506). Ubiquitously expressed with high expression in testis (PubMed:27485036).2 Publications

Inductioni

By IFNG and LPS in macrophages. By IL2 in natural killer cells and cytotoxic T-cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000028793
CleanExiMM_RNF19B
ExpressionAtlasiA2A7Q9 baseline and differential
GenevisibleiA2A7Q9 MM

Interactioni

Subunit structurei

Interacts with UBE2L3, UBE2L6 and UCKL1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217322, 2 interactors
STRINGi10090.ENSMUSP00000131373

Structurei

3D structure databases

ProteinModelPortaliA2A7Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 315Required for ubiquitin ligase activity and for protection against staurosporin-induced cell deathBy similarityAdd BLAST315

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 32Poly-Arg4
Compositional biasi52 – 87Pro-richAdd BLAST36

Domaini

The first IBR-type zinc finger is the most crucial for interaction with UBE2L3, UBE2L6 and UCKL1.By similarity
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF19 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri116 – 163RING-type 1PROSITE-ProRule annotationAdd BLAST48
Zinc fingeri183 – 248IBR-typeAdd BLAST66
Zinc fingeri284 – 315RING-type 2PROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000059258
HOVERGENiHBG052073
InParanoidiA2A7Q9
OMAiCEQKDCL
OrthoDBiEOG091G0503
PhylomeDBiA2A7Q9
TreeFamiTF324777

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

A2A7Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSEKDSESP RSTSLHAAAP DPKCRSGGRR RRLTFHSVFS ASARGRRART
60 70 80 90 100
KPQAEPPPPA APPPPPPPAP APVEAQAPPV EALPSEPAAE AEAEAVAAGP
110 120 130 140 150
EEDEAAEGGG AEEVECPLCL VRLPPERAPR LLSCPHRSCR DCLRHYLRLE
160 170 180 190 200
ISESRVPISC PECSERLNPH DIRLLLADPP LMHKYEEFML RRYLASDPDC
210 220 230 240 250
RWCPAPDCGY AVIAYGCASC PKLTCEREGC QTEFCYHCKQ IWHPNQTCDM
260 270 280 290 300
ARQQRAQTLR VRTKHTSGLS YGQESGPADD IKPCPRCSAY IIKMNDGSCN
310 320 330 340 350
HMTCAVCGCE FCWLCMKEIS DLHYLSPSGC TFWGKKPWSR KKKILWQLGT
360 370 380 390 400
LIGAPVGISL IAGIAIPAMV IGIPVYVGRK IHSRYEGRKT SKHKRNLAIT
410 420 430 440 450
GGVTLSVIAS PVIAAVSVGI GVPIMLAYVY GVVPISLCRG GGCGVSTANG
460 470 480 490 500
KGVKIEFDED DGPITVADAW RALKNPSIGE SSIEGLTSVL STSGSPTDGL
510 520 530 540 550
SVMQGPYSET ASFAALSGGT LSGGILSSGK GKYSRLEVQA DVQKEIFPKD
560 570 580 590 600
TASLGAISDS ASTRAMAGSI ISSYNPQDRE CNNMEIQVDI EAKPSHYQLV
610 620 630 640 650
SGSSTEDSLH VHAQVAEKEE EGNGAGGGSG GSEDDPPYKH QSCEQKDCLA
660 670 680 690 700
SKAWDISLAQ PESIRSDLES SDTQSDDVPD ITSDECGSPR SHAAACPSTP
710 720 730
QVHGAPSPSA HKNLAAPAEG QTVLKSEEYE VE
Length:732
Mass (Da):78,127
Last modified:October 23, 2007 - v2
Checksum:i81AAF64F9AE566F7
GO

Sequence cautioni

The sequence AAH52529 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAM19707 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti278Missing (PubMed:10912506).Curated1
Sequence conflicti278Missing in AAI17808 (PubMed:15489334).Curated1
Sequence conflicti721Q → P in AAH52529 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606977 Genomic DNA Translation: CAM19707.1 Different initiation.
BC052529 mRNA Translation: AAH52529.1 Different initiation.
BC117807 mRNA Translation: AAI17808.1
RefSeqiXP_006503521.1, XM_006503458.3
UniGeneiMm.259672
Mm.486170

Genome annotation databases

EnsembliENSMUST00000030584; ENSMUSP00000030584; ENSMUSG00000028793
GeneIDi75234
UCSCiuc008uvx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRN19B_MOUSE
AccessioniPrimary (citable) accession number: A2A7Q9
Secondary accession number(s): A0AUN9, Q7TT05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: April 25, 2018
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health