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Protein

E3 ubiquitin-protein ligase RNF19B

Gene

Rnf19b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri116 – 16348RING-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri183 – 24866IBR-typeAdd
BLAST
Zinc fingeri284 – 31532RING-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Adaptive immunity, Immunity, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF19B (EC:6.3.2.-)
Alternative name(s):
IBR domain-containing protein 3
Natural killer lytic-associated molecule
RING finger protein 19B
Gene namesi
Name:Rnf19b
Synonyms:Ibrdc3, Nklam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922484. Rnf19b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei351 – 37121HelicalSequence analysisAdd
BLAST
Transmembranei412 – 43221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytolytic granule Source: MGI
  • cytoplasm Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • ubiquitin ligase complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 732732E3 ubiquitin-protein ligase RNF19BPRO_0000307188Add
BLAST

Proteomic databases

PaxDbiA2A7Q9.
PRIDEiA2A7Q9.

PTM databases

iPTMnetiA2A7Q9.
PhosphoSiteiA2A7Q9.

Expressioni

Tissue specificityi

Expressed specifically in natural killer cells, activated macrophages and cytotoxic T-cells. Present in macrophages (at protein level).1 Publication

Inductioni

By IFNG and LPS in macrophages. By IL2 in natural killer cells and cytotoxic T-cells.1 Publication

Gene expression databases

BgeeiA2A7Q9.
CleanExiMM_RNF19B.
ExpressionAtlasiA2A7Q9. baseline and differential.
GenevisibleiA2A7Q9. MM.

Interactioni

Subunit structurei

Interacts with UBE2L3, UBE2L6 and UCKL1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000131373.

Structurei

3D structure databases

ProteinModelPortaliA2A7Q9.
SMRiA2A7Q9. Positions 116-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 324Poly-Arg
Compositional biasi52 – 8736Pro-richAdd
BLAST

Domaini

The first IBR-type zinc finger is the most crucial for interaction with UBE2L3, UBE2L6 and UCKL1.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF19 subfamily.Curated
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri116 – 16348RING-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri183 – 24866IBR-typeAdd
BLAST
Zinc fingeri284 – 31532RING-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129738.
HOGENOMiHOG000059258.
HOVERGENiHBG052073.
InParanoidiA2A7Q9.
OMAiWDITLAQ.
PhylomeDBiA2A7Q9.
TreeFamiTF324777.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2A7Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSEKDSESP RSTSLHAAAP DPKCRSGGRR RRLTFHSVFS ASARGRRART
60 70 80 90 100
KPQAEPPPPA APPPPPPPAP APVEAQAPPV EALPSEPAAE AEAEAVAAGP
110 120 130 140 150
EEDEAAEGGG AEEVECPLCL VRLPPERAPR LLSCPHRSCR DCLRHYLRLE
160 170 180 190 200
ISESRVPISC PECSERLNPH DIRLLLADPP LMHKYEEFML RRYLASDPDC
210 220 230 240 250
RWCPAPDCGY AVIAYGCASC PKLTCEREGC QTEFCYHCKQ IWHPNQTCDM
260 270 280 290 300
ARQQRAQTLR VRTKHTSGLS YGQESGPADD IKPCPRCSAY IIKMNDGSCN
310 320 330 340 350
HMTCAVCGCE FCWLCMKEIS DLHYLSPSGC TFWGKKPWSR KKKILWQLGT
360 370 380 390 400
LIGAPVGISL IAGIAIPAMV IGIPVYVGRK IHSRYEGRKT SKHKRNLAIT
410 420 430 440 450
GGVTLSVIAS PVIAAVSVGI GVPIMLAYVY GVVPISLCRG GGCGVSTANG
460 470 480 490 500
KGVKIEFDED DGPITVADAW RALKNPSIGE SSIEGLTSVL STSGSPTDGL
510 520 530 540 550
SVMQGPYSET ASFAALSGGT LSGGILSSGK GKYSRLEVQA DVQKEIFPKD
560 570 580 590 600
TASLGAISDS ASTRAMAGSI ISSYNPQDRE CNNMEIQVDI EAKPSHYQLV
610 620 630 640 650
SGSSTEDSLH VHAQVAEKEE EGNGAGGGSG GSEDDPPYKH QSCEQKDCLA
660 670 680 690 700
SKAWDISLAQ PESIRSDLES SDTQSDDVPD ITSDECGSPR SHAAACPSTP
710 720 730
QVHGAPSPSA HKNLAAPAEG QTVLKSEEYE VE
Length:732
Mass (Da):78,127
Last modified:October 23, 2007 - v2
Checksum:i81AAF64F9AE566F7
GO

Sequence cautioni

The sequence AAH52529.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAM19707.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti278 – 2781Missing (PubMed:10912506).Curated
Sequence conflicti278 – 2781Missing in AAI17808 (PubMed:15489334).Curated
Sequence conflicti721 – 7211Q → P in AAH52529 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606977 Genomic DNA. Translation: CAM19707.1. Different initiation.
BC052529 mRNA. Translation: AAH52529.1. Different initiation.
BC117807 mRNA. Translation: AAI17808.1.
RefSeqiXP_006503521.1. XM_006503458.2.
XP_006537091.1. XM_006537028.2.
XP_011238932.1. XM_011240630.1.
XP_011249548.1. XM_011251246.1.
UniGeneiMm.259672.
Mm.486170.

Genome annotation databases

EnsembliENSMUST00000030584; ENSMUSP00000030584; ENSMUSG00000028793.
GeneIDi75234.
UCSCiuc008uvx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606977 Genomic DNA. Translation: CAM19707.1. Different initiation.
BC052529 mRNA. Translation: AAH52529.1. Different initiation.
BC117807 mRNA. Translation: AAI17808.1.
RefSeqiXP_006503521.1. XM_006503458.2.
XP_006537091.1. XM_006537028.2.
XP_011238932.1. XM_011240630.1.
XP_011249548.1. XM_011251246.1.
UniGeneiMm.259672.
Mm.486170.

3D structure databases

ProteinModelPortaliA2A7Q9.
SMRiA2A7Q9. Positions 116-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000131373.

PTM databases

iPTMnetiA2A7Q9.
PhosphoSiteiA2A7Q9.

Proteomic databases

PaxDbiA2A7Q9.
PRIDEiA2A7Q9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030584; ENSMUSP00000030584; ENSMUSG00000028793.
GeneIDi75234.
UCSCiuc008uvx.2. mouse.

Organism-specific databases

CTDi127544.
MGIiMGI:1922484. Rnf19b.

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129738.
HOGENOMiHOG000059258.
HOVERGENiHBG052073.
InParanoidiA2A7Q9.
OMAiWDITLAQ.
PhylomeDBiA2A7Q9.
TreeFamiTF324777.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiRnf19b. mouse.
PROiA2A7Q9.
SOURCEiSearch...

Gene expression databases

BgeeiA2A7Q9.
CleanExiMM_RNF19B.
ExpressionAtlasiA2A7Q9. baseline and differential.
GenevisibleiA2A7Q9. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure of human and mouse NKLAM, a gene associated with cellular cytotoxicity."
    Portis T., Anderson J., Esposito A., Kornbluth J.
    Immunogenetics 51:546-555(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY CYTOKINES.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-732.
    Strain: Czech II.
    Tissue: Lung.

Entry informationi

Entry nameiRN19B_MOUSE
AccessioniPrimary (citable) accession number: A2A7Q9
Secondary accession number(s): A0AUN9, Q7TT05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: July 6, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.