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Protein

Cell division cycle protein 27 homolog

Gene

Cdc27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-MMU-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-MMU-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-MMU-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-MMU-176412. Phosphorylation of the APC/C.
R-MMU-179409. APC-Cdc20 mediated degradation of Nek2A.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 27 homolog
Gene namesi
Name:Cdc27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:102685. Cdc27.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Cell division cycle protein 27 homologPRO_0000390473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei205 – 2051PhosphothreonineBy similarity
Modified residuei209 – 2091PhosphothreonineBy similarity
Modified residuei291 – 2911PhosphoserineBy similarity
Modified residuei313 – 3131PhosphothreonineBy similarity
Modified residuei339 – 3391PhosphoserineBy similarity
Modified residuei367 – 3671PhosphothreonineBy similarity
Modified residuei387 – 3871PhosphoserineBy similarity
Modified residuei427 – 4271PhosphoserineBy similarity
Modified residuei431 – 4311PhosphothreonineBy similarity
Modified residuei436 – 4361PhosphoserineBy similarity
Modified residuei439 – 4391PhosphoserineBy similarity
Modified residuei447 – 4471PhosphothreonineBy similarity
Modified residuei822 – 8221PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylation on Ser-427 and Thr-447 occurs specifically during mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2A6Q5.
MaxQBiA2A6Q5.
PaxDbiA2A6Q5.
PRIDEiA2A6Q5.

PTM databases

iPTMnetiA2A6Q5.

Expressioni

Gene expression databases

BgeeiA2A6Q5.
ExpressionAtlasiA2A6Q5. baseline and differential.
GenevisibleiA2A6Q5. MM.

Interactioni

Subunit structurei

Homodimer. The mammalian APC/C is composed of 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Interacts with RB (By similarity). Interacts with MCPH1 (By similarity). Interacts with FAM168B/MANI.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229869. 6 interactions.
IntActiA2A6Q5. 4 interactions.
STRINGi10090.ENSMUSP00000091452.

Structurei

3D structure databases

ProteinModelPortaliA2A6Q5.
SMRiA2A6Q5. Positions 5-171, 452-783.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati6 – 3530TPR 1Add
BLAST
Repeati38 – 6528TPR 2Add
BLAST
Repeati67 – 9933TPR 3Add
BLAST
Repeati115 – 14531TPR 4Add
BLAST
Repeati465 – 49531TPR 5Add
BLAST
Repeati499 – 52830TPR 6Add
BLAST
Repeati533 – 56331TPR 7Add
BLAST
Repeati567 – 59832TPR 8Add
BLAST
Repeati601 – 63131TPR 9Add
BLAST
Repeati635 – 66733TPR 10Add
BLAST
Repeati670 – 70233TPR 11Add
BLAST
Repeati704 – 73431TPR 12Add
BLAST
Repeati737 – 76832TPR 13Add
BLAST

Sequence similaritiesi

Belongs to the APC3/CDC27 family.Curated
Contains 13 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1126. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00550000074887.
HOGENOMiHOG000231056.
HOVERGENiHBG050859.
InParanoidiA2A6Q5.
KOiK03350.
OMAiNSCTTQV.
OrthoDBiEOG751NDX.
PhylomeDBiA2A6Q5.
TreeFamiTF101058.

Family and domain databases

InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 8 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2A6Q5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR
60 70 80 90 100
SGKAYKAYRL LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ
110 120 130 140 150
KSHDDLVTEF GDSACFTLSL LGHVYCKTDR LAKGSECYQK SLSLNPFLWS
160 170 180 190 200
PFESLCEIGE KPDPDQTFKL TSLQNFSSCL PNTCTTLVSN HSLSHRQPET
210 220 230 240 250
VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSTVI SPDNVPLGPG
260 270 280 290 300
TAILSKQVQN KPKTGRSLLG GPTALSPLTP SFGILPLETP SPGDGSYLQN
310 320 330 340 350
YTNTPSVIDV APTGAPTKKS VARMGQTGTK SVFSQSGNSR EVTPVLVAQT
360 370 380 390 400
QSSGPQTSTT PQVLSPTITS PPNALPRRSS RLFTSDSSTT KENSKKLKMK
410 420 430 440 450
FPPKIPNRKT KSKTNKGGLT QPSINDSLEI TKLDSSIISE GKITTVTPQI
460 470 480 490 500
QAFNLQKAAA EGLMSLLREM GKGYLALCSY NCKEAINILS HLPSHHYSTG
510 520 530 540 550
WVLCQIGRAY FELSEYMQAE RIFSEVRRIE SFRVEGMEIY STTLWHLQKD
560 570 580 590 600
VALSVLSKDL TDMDKNSPEA WCAAGNCFSL QREHDIAIKF FQRAIQVDPN
610 620 630 640 650
YAYAYTLLGH EFVLTEELDK ALACFRNAIR VNPRHYNAWY GLGMIYYKQE
660 670 680 690 700
KFSLAEMHFQ KALDINPQSS VLLCHIGVVQ HALKKSEKAL DTLNKAIVID
710 720 730 740 750
PKNPLCKFHR ASVLFANEKY KSALQELEEL KQIVPKESLV YFLIGKVYKK
760 770 780 790 800
LGQTHLALMN FSWAMDLDPK GANNQIKEAI DKRYLPDDEE PITQEEQIMG
810 820
TDESQESSMT DADDTQLHAA ESDEF
Length:825
Mass (Da):91,842
Last modified:February 20, 2007 - v1
Checksum:i554D8B1136826DA3
GO
Isoform 2 (identifier: A2A6Q5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-319: K → KTFCVLQ
     461-461: Missing.

Note: No experimental confirmation available.
Show »
Length:830
Mass (Da):92,405
Checksum:i28AD6139A08823F5
GO
Isoform 3 (identifier: A2A6Q5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-319: K → KTFCVLQ

Note: No experimental confirmation available.
Show »
Length:831
Mass (Da):92,534
Checksum:iFCFAEA4338F99917
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei319 – 3191K → KTFCVLQ in isoform 2 and isoform 3. 1 PublicationVSP_038534
Alternative sequencei461 – 4611Missing in isoform 2. CuratedVSP_038535

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603709 Genomic DNA. Translation: CAM22621.1.
AL603709 Genomic DNA. Translation: CAM22622.1.
CH466558 Genomic DNA. Translation: EDL34221.1.
BC157955 mRNA. Translation: AAI57956.1.
BC172100 mRNA. Translation: AAI72100.1.
CCDSiCCDS36354.1. [A2A6Q5-1]
RefSeqiNP_001272917.1. NM_001285988.1. [A2A6Q5-2]
NP_663411.2. NM_145436.2. [A2A6Q5-1]
XP_006533113.1. XM_006533050.1.
UniGeneiMm.89845.

Genome annotation databases

EnsembliENSMUST00000093923; ENSMUSP00000091452; ENSMUSG00000020687. [A2A6Q5-1]
ENSMUST00000106962; ENSMUSP00000102575; ENSMUSG00000020687. [A2A6Q5-3]
GeneIDi217232.
KEGGimmu:217232.
UCSCiuc007lwq.2. mouse. [A2A6Q5-1]
uc007lwr.2. mouse. [A2A6Q5-2]
uc011ygc.2. mouse. [A2A6Q5-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603709 Genomic DNA. Translation: CAM22621.1.
AL603709 Genomic DNA. Translation: CAM22622.1.
CH466558 Genomic DNA. Translation: EDL34221.1.
BC157955 mRNA. Translation: AAI57956.1.
BC172100 mRNA. Translation: AAI72100.1.
CCDSiCCDS36354.1. [A2A6Q5-1]
RefSeqiNP_001272917.1. NM_001285988.1. [A2A6Q5-2]
NP_663411.2. NM_145436.2. [A2A6Q5-1]
XP_006533113.1. XM_006533050.1.
UniGeneiMm.89845.

3D structure databases

ProteinModelPortaliA2A6Q5.
SMRiA2A6Q5. Positions 5-171, 452-783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229869. 6 interactions.
IntActiA2A6Q5. 4 interactions.
STRINGi10090.ENSMUSP00000091452.

PTM databases

iPTMnetiA2A6Q5.

Proteomic databases

EPDiA2A6Q5.
MaxQBiA2A6Q5.
PaxDbiA2A6Q5.
PRIDEiA2A6Q5.

Protocols and materials databases

DNASUi217232.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093923; ENSMUSP00000091452; ENSMUSG00000020687. [A2A6Q5-1]
ENSMUST00000106962; ENSMUSP00000102575; ENSMUSG00000020687. [A2A6Q5-3]
GeneIDi217232.
KEGGimmu:217232.
UCSCiuc007lwq.2. mouse. [A2A6Q5-1]
uc007lwr.2. mouse. [A2A6Q5-2]
uc011ygc.2. mouse. [A2A6Q5-3]

Organism-specific databases

CTDi996.
MGIiMGI:102685. Cdc27.

Phylogenomic databases

eggNOGiKOG1126. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00550000074887.
HOGENOMiHOG000231056.
HOVERGENiHBG050859.
InParanoidiA2A6Q5.
KOiK03350.
OMAiNSCTTQV.
OrthoDBiEOG751NDX.
PhylomeDBiA2A6Q5.
TreeFamiTF101058.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-MMU-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-MMU-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-MMU-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-MMU-176412. Phosphorylation of the APC/C.
R-MMU-179409. APC-Cdc20 mediated degradation of Nek2A.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi375691.
PROiA2A6Q5.
SOURCEiSearch...

Gene expression databases

BgeeiA2A6Q5.
ExpressionAtlasiA2A6Q5. baseline and differential.
GenevisibleiA2A6Q5. MM.

Family and domain databases

InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 8 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 2 AND 3).
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  4. "The novel protein MANI modulates neurogenesis and neurite-cone growth."
    Mishra M., Akatsu H., Heese K.
    J. Cell. Mol. Med. 15:1713-1725(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FAM168B.

Entry informationi

Entry nameiCDC27_MOUSE
AccessioniPrimary (citable) accession number: A2A6Q5
Secondary accession number(s): A2A6Q6, B2RXS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: February 20, 2007
Last modified: May 11, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.