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Protein

Calcium-binding and coiled-coil domain-containing protein 2

Gene

Calcoco2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation (By similarity). Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens upon entry into the cytosol by targeting galectin-8-associated bacteria for autophagy (By similarity). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (By similarity). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (By similarity). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding and coiled-coil domain-containing protein 2By similarity
Alternative name(s):
Nuclear domain 10 protein NDP52By similarity
Short name:
Nuclear domain 10 protein 52By similarity
Gene namesi
Name:Calcoco2By similarity
Synonyms:Ndp52, Ndp52l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1343177. Calcoco2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Calcium-binding and coiled-coil domain-containing protein 2PRO_0000312339Add
BLAST

Proteomic databases

PaxDbiA2A6M5.
PRIDEiA2A6M5.

PTM databases

PhosphoSiteiA2A6M5.

Expressioni

Developmental stagei

Expression is limited to the pluripotent cells of the early embryo and the germline. Expressed in blastocysts, epiblasts and purified primordial germ cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000006056.
CleanExiMM_CALCOCO2.
ExpressionAtlasiA2A6M5. baseline and differential.
GenevisibleiA2A6M5. MM.

Interactioni

Subunit structurei

Dimer. Part of a complex consisting of CALCOCO2, TAX1BP1 and MYO6. Interacts with GEMIN4. Interacts with ATG8 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2. Interacts with ATG8 family member MAP1LC3C. Interacts with LGALS8.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087407.

Structurei

3D structure databases

ProteinModelPortaliA2A6M5.
SMRiA2A6M5. Positions 16-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni292 – 30211Interaction with LGALS8By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili132 – 309178Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi128 – 1314CLIRBy similarity
Motifi190 – 1934LIR-likeBy similarity

Domaini

The LGALS8-binding domain is essential for the recruitment to cytosol-exposed infecting bacteria.By similarity
The CLIR (LC3C-interacting region) motif is required for interaction with MAP1LC3C, but dispensable for CALCOCO2-mediated autophagosome maturation.By similarity
The LIR-like motif is required for interaction with MAP1LC3A, MAP1LC3B and GABARAPL2, as well as for CALCOCO2-mediated autophagosome maturation.By similarity

Sequence similaritiesi

Belongs to the CALCOCO family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IKI3. Eukaryota.
ENOG4112B0R. LUCA.
GeneTreeiENSGT00530000063216.
HOGENOMiHOG000111284.
InParanoidiA2A6M5.
OrthoDBiEOG091G01W2.
PhylomeDBiA2A6M5.
TreeFamiTF329501.

Family and domain databases

InterProiIPR012852. CALCOCO1-like.
[Graphical view]
PfamiPF07888. CALCOCO1. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2A6M5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDQCPIPTLL EHGNFSQVLF NNVEKFYAPR GDIMCYYTLT EKFIPRRKDW
60 70 80 90 100
IGIFKVGWKT TQEYYTFMWA PLPKDQNKDS ATQQEIQFKA YYLPKDVERY
110 120 130 140 150
QFCYVDEDGL VRGTSVPFQF CPDPDEDIMV VINKEKVEEM EQLSEELYQQ
160 170 180 190 200
NQELKDKYAD LHEQLQRKQV ALEATQRVNK TLEHKVEEKA SWEKEKASWE
210 220 230 240 250
EEKASWEEEK ASWEEEKASW EEEKASWEEE KASWEEEKAS WEEEKASWEE
260 270 280 290 300
EKASWEEEKA SWEEEKASWE EEKASWEEEK ASWEEEKASW EKEKASWEEE
310 320 330
KASWEKEKAP WEVEKAPWKE VKAYWWNDLH R
Length:331
Mass (Da):40,136
Last modified:February 20, 2007 - v1
Checksum:iA47DAF67DCB89A74
GO
Isoform 2 (identifier: A2A6M5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-291: Missing.

Show »
Length:261
Mass (Da):31,537
Checksum:i806ABB1BEAA9B755
GO
Isoform 3 (identifier: A2A6M5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-320: Missing.

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):28,110
Checksum:i649C1B82DCB75536
GO
Isoform 4 (identifier: A2A6M5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-291: Missing.

Note: No experimental confirmation available.
Show »
Length:240
Mass (Da):28,958
Checksum:i2D995299795D5D2E
GO
Isoform 5 (identifier: A2A6M5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     208-305: Missing.

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):28,099
Checksum:i6492E4B641033B69
GO

Sequence cautioni

The sequence BAE38997 differs from that shown. Reason: Frameshift at position 193. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei201 – 29191Missing in isoform 4. 1 PublicationVSP_029830Add
BLAST
Alternative sequencei208 – 30598Missing in isoform 5. 1 PublicationVSP_029831Add
BLAST
Alternative sequencei222 – 29170Missing in isoform 2. 2 PublicationsVSP_029832Add
BLAST
Alternative sequencei223 – 32098Missing in isoform 3. 1 PublicationVSP_029833Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490344 mRNA. Translation: AAO84502.1.
AK010816 mRNA. Translation: BAB27200.1.
AK166710 mRNA. Translation: BAE38963.1.
AK166756 mRNA. Translation: BAE38997.1. Frameshift.
AK167172 mRNA. Translation: BAE39309.1.
AL603682 Genomic DNA. Translation: CAM18272.1.
CCDSiCCDS59567.1. [A2A6M5-1]
RefSeqiNP_001257947.1. NM_001271018.1. [A2A6M5-1]
UniGeneiMm.296049.

Genome annotation databases

EnsembliENSMUST00000068686; ENSMUSP00000087407; ENSMUSG00000006056. [A2A6M5-1]
GeneIDi76815.
KEGGimmu:76815.
UCSCiuc033fzk.1. mouse. [A2A6M5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490344 mRNA. Translation: AAO84502.1.
AK010816 mRNA. Translation: BAB27200.1.
AK166710 mRNA. Translation: BAE38963.1.
AK166756 mRNA. Translation: BAE38997.1. Frameshift.
AK167172 mRNA. Translation: BAE39309.1.
AL603682 Genomic DNA. Translation: CAM18272.1.
CCDSiCCDS59567.1. [A2A6M5-1]
RefSeqiNP_001257947.1. NM_001271018.1. [A2A6M5-1]
UniGeneiMm.296049.

3D structure databases

ProteinModelPortaliA2A6M5.
SMRiA2A6M5. Positions 16-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087407.

PTM databases

PhosphoSiteiA2A6M5.

Proteomic databases

PaxDbiA2A6M5.
PRIDEiA2A6M5.

Protocols and materials databases

DNASUi76815.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068686; ENSMUSP00000087407; ENSMUSG00000006056. [A2A6M5-1]
GeneIDi76815.
KEGGimmu:76815.
UCSCiuc033fzk.1. mouse. [A2A6M5-1]

Organism-specific databases

CTDi10241.
MGIiMGI:1343177. Calcoco2.

Phylogenomic databases

eggNOGiENOG410IKI3. Eukaryota.
ENOG4112B0R. LUCA.
GeneTreeiENSGT00530000063216.
HOGENOMiHOG000111284.
InParanoidiA2A6M5.
OrthoDBiEOG091G01W2.
PhylomeDBiA2A6M5.
TreeFamiTF329501.

Miscellaneous databases

PROiA2A6M5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006056.
CleanExiMM_CALCOCO2.
ExpressionAtlasiA2A6M5. baseline and differential.
GenevisibleiA2A6M5. MM.

Family and domain databases

InterProiIPR012852. CALCOCO1-like.
[Graphical view]
PfamiPF07888. CALCOCO1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCACO2_MOUSE
AccessioniPrimary (citable) accession number: A2A6M5
Secondary accession number(s): Q3TK36
, Q3TKZ6, Q3TL30, Q9CWE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: February 20, 2007
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.