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A2A5R2

- BIG2_MOUSE

UniProt

A2A5R2 - BIG2_MOUSE

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Protein

Brefeldin A-inhibited guanine nucleotide-exchange protein 2

Gene

Arfgef2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extend on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways (By similarity).By similarity

Enzyme regulationi

Inhibited by brefeldin A.By similarity

GO - Molecular functioni

  1. ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
  2. GABA receptor binding Source: UniProtKB
  3. protein kinase A regulatory subunit binding Source: UniProtKB

GO - Biological processi

  1. endomembrane system organization Source: UniProtKB
  2. endosome organization Source: UniProtKB
  3. exocytosis Source: Ensembl
  4. Golgi to plasma membrane transport Source: UniProtKB
  5. intracellular signal transduction Source: Ensembl
  6. positive regulation of GTPase activity Source: GOC
  7. positive regulation of tumor necrosis factor production Source: UniProtKB
  8. protein transport Source: UniProtKB-KW
  9. receptor recycling Source: UniProtKB
  10. regulation of ARF protein signal transduction Source: InterPro
  11. vesicle-mediated transport Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_244970. Association of TriC/CCT with target proteins during biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 2
Short name:
Brefeldin A-inhibited GEP 2
Alternative name(s):
ADP-ribosylation factor guanine nucleotide-exchange factor 2
Gene namesi
Name:Arfgef2
Synonyms:Arfgep2, Big2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2139354. Arfgef2.

Subcellular locationi

Cytoplasm By similarity. Membrane By similarity. Golgi apparatus By similarity. Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Endosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cell projectiondendrite By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapse By similarity. Cytoplasmcytoskeleton By similarity
Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes (By similarity).By similarity

GO - Cellular componenti

  1. asymmetric synapse Source: UniProtKB
  2. axonemal microtubule Source: UniProtKB
  3. cell junction Source: UniProtKB-KW
  4. cytoplasmic vesicle Source: UniProtKB
  5. cytosol Source: Ensembl
  6. dendritic spine Source: UniProtKB
  7. Golgi membrane Source: UniProtKB
  8. microtubule organizing center Source: UniProtKB
  9. recycling endosome Source: UniProtKB
  10. symmetric synapse Source: UniProtKB
  11. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17921792Brefeldin A-inhibited guanine nucleotide-exchange protein 2PRO_0000419332Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei218 – 2181Phosphoserine1 Publication
Modified residuei227 – 2271Phosphoserine1 Publication
Modified residuei621 – 6211Phosphoserine1 Publication
Modified residuei623 – 6231Phosphothreonine1 Publication
Modified residuei1532 – 15321PhosphoserineBy similarity
Modified residuei1535 – 15351PhosphoserineBy similarity
Modified residuei1789 – 17891PhosphoserineBy similarity

Post-translational modificationi

In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiA2A5R2.
PaxDbiA2A5R2.
PRIDEiA2A5R2.

PTM databases

PhosphoSiteiA2A5R2.

Expressioni

Gene expression databases

BgeeiA2A5R2.
ExpressionAtlasiA2A5R2. baseline and differential.
GenevestigatoriA2A5R2.

Interactioni

Subunit structurei

Homodimer. Interacts with BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with PRKAR1A, PRKAR2A, PRKAR1B, PRKAR2B, PPP1CC, PDE3A, TNFRSF1A, MYCBP and EXOC7. Interacts with GABRB1, GABRB2 and GABRB3 (By similarity).By similarity

Protein-protein interaction databases

IntActiA2A5R2. 1 interaction.
STRINGi10090.ENSMUSP00000096677.

Structurei

3D structure databases

ProteinModelPortaliA2A5R2.
SMRiA2A5R2. Positions 644-835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini661 – 792132SEC7PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 224223DCB; DCB:DCB domain and DCB:HUS domain interactionBy similarityAdd
BLAST
Regioni515 – 53521HUS; DCB:HUS domain interactionBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5307.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000181045.
HOVERGENiHBG004846.
InParanoidiA2A5R2.
KOiK18442.
OMAiEQGMLGA.
OrthoDBiEOG7QVM1S.
PhylomeDBiA2A5R2.
TreeFamiTF300714.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015403. DUF1981_Sec7_assoc.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2A5R2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQESQTKSMF VSRALEKILA DKEVKRPQHS QLRRACQVAL DEIKAELEKQ
60 70 80 90 100
RLGAAAPPKA NFIEADKYFL PFELACQSKS PRVVSTSLDC LQKLIAYGHI
110 120 130 140 150
TGNAPDSGAP GKRLIDRIVE TICNCFQGPQ TDEGVQLQII KALLTAVTSP
160 170 180 190 200
HIEIHEGTIL QTVRTCYNIY LASKNLINQT TAKATLTQML NVIFTRMENQ
210 220 230 240 250
VLQEARELEK PMQSKPQSPV IQATAGSPKF SRLKQSQAQS KPTTPEKAEL
260 270 280 290 300
PNGDHAQSGL GKVSLENGEA PRERGSPVSG RAEPSRGTDS GAQEVVKDIL
310 320 330 340 350
EDVVTSAVKE AAEKHGLPEP DRALGALECQ ECAVPPGVDE NSQTNGIADD
360 370 380 390 400
RQSLSSADNL EPDVQGHQVA ARFSHILQKD AFLVFRSLCK LSMKPLGEGP
410 420 430 440 450
PDPKSHELRS KVVSLQLLLS VLQNAGPVFR SHEMFVTAIK QYLCVALSKN
460 470 480 490 500
GVSSVPDVFE LSLAIFLTLL SNFKMHLKMQ IEVFFKEIFL NILETSTSSF
510 520 530 540 550
EHRWMVIQTL TRICADAQCV VDIYVNYDCD LNAANIFERL VNDLSKIAQG
560 570 580 590 600
RSGHELGMTP LQELSLRKKG LECLVSILKC MVEWSKDLYV NPNHQATLGQ
610 620 630 640 650
ERLPDQEMGD GKGLDMARRC SVTSVESTVS SGTQTAIQDD PEQFEVIKQQ
660 670 680 690 700
KEIIEHGIEL FNKKPKRGIQ FLQEQGMLGA AVEDIAQFLH QEERLDSTQV
710 720 730 740 750
GEFLGDSTRF NKEVMYAYVD QLDFCEKEFV SALRTFLEGF RLPGEAQKID
760 770 780 790 800
RLMEKFAARY IECNQGQTLF ASADTAYVLA YSIIMLTTDL HSPQVKNKMT
810 820 830 840 850
KEQYIKMNRG INDSKDLPEE YLSSIYDEIE GKKIAMKETK EHTIATKSTK
860 870 880 890 900
QSVASEKQRR LLYNVEMEQM AKTAKALMEA VSHAKAPFTS ATHLDHVRPM
910 920 930 940 950
FKLVWTPLLA AYSIGLQNCD DTEVASLCLE GIRCAVRIAC IFGMQLERDA
960 970 980 990 1000
YVQALARFSL LTASSSITEM KQKNIDTIKT LITVAHTDGN YLGNSWHEIL
1010 1020 1030 1040 1050
KCISQLELAQ LIGTGVKTRY LSGSGREREG SLKGHSLAGE EFMGLGLGNL
1060 1070 1080 1090 1100
VSGGVDKRQM ASFQESVGET SSQSVVVAVD RIFTGSTRLD GNAIVDFVRW
1110 1120 1130 1140 1150
LCAVSMDELA SPHHPRMFSL QKIVEISYYN MNRIRLQWSR IWHVIGDHFN
1160 1170 1180 1190 1200
KVGCNPNEDV AIFAVDSLRQ LSMKFLEKGE LANFRFQKDF LRPFEHIMKK
1210 1220 1230 1240 1250
NRSPTIRDMV IRCIAQMVSS QAANIRSGWK NIFAVFHQAA SDHDGNIVEL
1260 1270 1280 1290 1300
AFQTTGHIVS TIFQHHFPAA IDSFQDAVKC LSEFACNAAF PDTSMEAIRL
1310 1320 1330 1340 1350
IRFCGKYVSE RPRVLQEYTS DDMNVAPGDR VWVRGWFPIL FELSCIINRC
1360 1370 1380 1390 1400
KLDVRTRGLT VMFEIMKSYG HTFAKHWWQD LFRIVFRIFD NMKLPEQQSE
1410 1420 1430 1440 1450
KSEWMTTTCN HALYAICDVF TQFYEALHEV LLSDVFAQLQ WCVKQDNEQL
1460 1470 1480 1490 1500
ARSGTNCLEN LVISNGEKFS PAVWDETCNC MLDIFKTTIP HVLLTWRPAG
1510 1520 1530 1540 1550
MEEEVSDRHL DVDLDRQSLS SIDRNASERG QSQLSNPTDD SWKGAPYAHQ
1560 1570 1580 1590 1600
KLLASLLIKC VVQLELIQTI DNIVFYPATS KKEDAEHMVA AQQDTLDAEI
1610 1620 1630 1640 1650
HIETENQGMY KFMSSQHLFK LLDCLQESHS FSKAFNSNYE QRTVLWRAGF
1660 1670 1680 1690 1700
KGKSKPNLLK QETSSLACCL RILFRMYVDE NRRDSWDEIQ QRLLRVCSEA
1710 1720 1730 1740 1750
LAYFITVNSE SHREAWTSLL LLLLTKTLKI SDEKFKAHAS MYYPYLCEIM
1760 1770 1780 1790
QFDLIPELRA VLRKFFLRIG LVYKIWIPEE PSQVPAALSS TW
Length:1,792
Mass (Da):202,240
Last modified:February 20, 2007 - v1
Checksum:i23DF06EA23A34A71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591703, AL591911 Genomic DNA. Translation: CAM24646.1.
AL591911 Genomic DNA. Translation: CAM17723.1.
CH466551 Genomic DNA. Translation: EDL06489.1.
BC158012 mRNA. Translation: AAI58013.1.
CCDSiCCDS38335.1.
RefSeqiNP_001078964.1. NM_001085495.2.
UniGeneiMm.297192.
Mm.488805.

Genome annotation databases

EnsembliENSMUST00000099078; ENSMUSP00000096677; ENSMUSG00000074582.
GeneIDi99371.
KEGGimmu:99371.
UCSCiuc008nyr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591703 , AL591911 Genomic DNA. Translation: CAM24646.1 .
AL591911 Genomic DNA. Translation: CAM17723.1 .
CH466551 Genomic DNA. Translation: EDL06489.1 .
BC158012 mRNA. Translation: AAI58013.1 .
CCDSi CCDS38335.1.
RefSeqi NP_001078964.1. NM_001085495.2.
UniGenei Mm.297192.
Mm.488805.

3D structure databases

ProteinModelPortali A2A5R2.
SMRi A2A5R2. Positions 644-835.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi A2A5R2. 1 interaction.
STRINGi 10090.ENSMUSP00000096677.

PTM databases

PhosphoSitei A2A5R2.

Proteomic databases

MaxQBi A2A5R2.
PaxDbi A2A5R2.
PRIDEi A2A5R2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000099078 ; ENSMUSP00000096677 ; ENSMUSG00000074582 .
GeneIDi 99371.
KEGGi mmu:99371.
UCSCi uc008nyr.2. mouse.

Organism-specific databases

CTDi 10564.
MGIi MGI:2139354. Arfgef2.

Phylogenomic databases

eggNOGi COG5307.
GeneTreei ENSGT00760000119036.
HOGENOMi HOG000181045.
HOVERGENi HBG004846.
InParanoidi A2A5R2.
KOi K18442.
OMAi EQGMLGA.
OrthoDBi EOG7QVM1S.
PhylomeDBi A2A5R2.
TreeFami TF300714.

Enzyme and pathway databases

Reactomei REACT_244970. Association of TriC/CCT with target proteins during biosynthesis.

Miscellaneous databases

ChiTaRSi Arfgef2. mouse.
NextBioi 353893.
PROi A2A5R2.
SOURCEi Search...

Gene expression databases

Bgeei A2A5R2.
ExpressionAtlasi A2A5R2. baseline and differential.
Genevestigatori A2A5R2.

Family and domain databases

Gene3Di 1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015403. DUF1981_Sec7_assoc.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view ]
Pfami PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view ]
SMARTi SM00222. Sec7. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEi PS50190. SEC7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218; SER-227; SER-621 AND THR-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiBIG2_MOUSE
AccessioniPrimary (citable) accession number: A2A5R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: February 20, 2007
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3