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Protein

Lethal(3)malignant brain tumor-like protein 1

Gene

L3mbtl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei429Mediates recognition of monomethylated and dimethylated peptidesBy similarity1
Sitei432Positioned at the entrance of MBT 2 and is required for recognition of monomethylated and dimethylated peptidesBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri619 – 662CCHHC-typePROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6804760 Regulation of TP53 Activity through Methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Lethal(3)malignant brain tumor-like protein 1
Short name:
H-l(3)mbt
Short name:
H-l(3)mbt protein
Short name:
L(3)mbt-like
Alternative name(s):
L(3)mbt protein homolog
Gene namesi
Name:L3mbtl1
Synonyms:Kiaa0681, L3mbt, L3mbtl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2676663 L3mbtl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice develop and reproduc normally. Mice were followed for more than 2 years, without any alteration in normal lifespan or survival with or without sublethal irradiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004058311 – 826Lethal(3)malignant brain tumor-like protein 1Add BLAST826

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated in a VCP/p97-dependent way following DNA damage, leading to its removal from DNA damage sites, promoting accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited to DNA damage sites.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiA2A5N8
PRIDEiA2A5N8

PTM databases

iPTMnetiA2A5N8
PhosphoSitePlusiA2A5N8

Expressioni

Tissue specificityi

Highly expressed in brain, testis, eyes, and ES cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000035576
ExpressionAtlasiA2A5N8 baseline and differential

Interactioni

Subunit structurei

Homodimer. Interacts with RB1/RB (when monomethylated at 'Lys-860'). Interacts with p53/TP53 (when monomethylated at 'Lys-382'). Interacts with CBX3, ETV6, KMT5A and VCP/p97 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044038

Structurei

3D structure databases

ProteinModelPortaliA2A5N8
SMRiA2A5N8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati280 – 380MBT 1Add BLAST101
Repeati388 – 487MBT 2Add BLAST100
Repeati496 – 591MBT 3Add BLAST96
Domaini757 – 821SAMPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni453 – 460Interaction with monomethylated and dimethylated peptidesBy similarity8

Domaini

The MBT repeat 2 specifically recognizes and binds monomethylated and dimethylated proteins. In contrast, it does not bind trimethylated proteins. The MBT repeat 1 does not bind methylated peptides but inserts a proline ring in a Pro-Ser-Ser/Thr sequence context (By similarity).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri619 – 662CCHHC-typePROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3766 Eukaryota
ENOG410Y4AQ LUCA
GeneTreeiENSGT00760000119024
HOGENOMiHOG000231019
HOVERGENiHBG071375
InParanoidiA2A5N8
OMAiRFTAHYC
OrthoDBiEOG091G01BW
PhylomeDBiA2A5N8
TreeFamiTF316498

Family and domain databases

InterProiView protein in InterPro
IPR004092 Mbt
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR002515 Znf_C2HC
IPR036060 Znf_C2HC_sf
PfamiView protein in Pfam
PF02820 MBT, 3 hits
PF00536 SAM_1, 1 hit
PF01530 zf-C2HC, 1 hit
SMARTiView protein in SMART
SM00561 MBT, 3 hits
SM00454 SAM, 1 hit
SUPFAMiSSF103637 SSF103637, 1 hit
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS51079 MBT, 3 hits
PS50105 SAM_DOMAIN, 1 hit
PS51802 ZF_CCHHC, 1 hit

Sequencei

Sequence statusi: Complete.

A2A5N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGHTDMEIL RTVKGSSTGE VNVHLVARDS AGPHPQLPTT AFIIPTNAAT
60 70 80 90 100
LGLPSTALDV PYPREPVHVG ALERVAGSEP VTATILPQLS TGTGTNSTVR
110 120 130 140 150
LLDWTGVSAP LPGSGMRFRI NEYAPLNMIG VERPRSPEQR HEGGMARRDA
160 170 180 190 200
GIQHPDVHQD RQDITSLEPP VDASSCKCQA CGPQQSSGLD VGSSGDRCSQ
210 220 230 240 250
PFQKRSVIVE NSGCTIASEL LKPMKKRKHK EYQSPSEESE PEAVKQGEGK
260 270 280 290 300
DAEREPTPST PENEEWSRSQ LVSSEKKDGW SWESYLEEQK AVTAPVSLFQ
310 320 330 340 350
DSQAVTHNKN GFKLGMKLEG IDPQHPSMYF ILTVAEVCGY RLRLHFDGYS
360 370 380 390 400
ECHDFWVNAN SPDIHPAGWF EKTGHKLQLP KGYKEEEFSW SQYLRSTKAQ
410 420 430 440 450
AAPKHLFVSQ SHSTPPVGFQ VGMKLEAVDR MNPSLVCVAS VTDVVDSRFL
460 470 480 490 500
VHFDDWGDTY DYWCDPSSPY IHPVGWCQKQ GKPLTPPQDY PDPDSFCWEK
510 520 530 540 550
YLEETGTSAV PNWAFKVRPP HSFLVNMKLE AVDRRNPALI RVASVEDVED
560 570 580 590 600
HRIKLHFDGW SHNYDFWIDA DHPDIHPAGW CSKTGHPLEP PLRPRESSSV
610 620 630 640 650
SPGGCPPLSH RSPPHTKTSK YNFHHRKCPT PGCDGSGHVT GKFTAHHCLS
660 670 680 690 700
GCPLAEKNQS RLKAELSDSE TAARKKNPSN LSPRKKPRHQ GRIGRPPKYR
710 720 730 740 750
KIPEEDLQAL PPSVVHQSLF MSTLPTHADR PLSVCWEQHC KLLPGVAGIS
760 770 780 790 800
ASTVSKWTIE EVFGFVQTLT GSEDQARLFK DEMIDGEAFL LLTQADIVKI
810 820
MSVKLGPALK IYNAILMFKN TDDAFK
Length:826
Mass (Da):91,781
Last modified:February 20, 2007 - v1
Checksum:iF27CE3D490434A44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti824A → V in BAD90413 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220350 mRNA Translation: BAD90413.1
AL606473, AL591584 Genomic DNA Translation: CAM20950.1
AL591584, AL606473 Genomic DNA Translation: CAM21343.1
CH466551 Genomic DNA Translation: EDL06309.1
CCDSiCCDS38314.1
RefSeqiNP_001074807.1, NM_001081338.1
XP_011237848.1, XM_011239546.2
UniGeneiMm.187086

Genome annotation databases

EnsembliENSMUST00000035751; ENSMUSP00000044038; ENSMUSG00000035576
GeneIDi241764
KEGGimmu:241764
UCSCiuc008nsd.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLMBL1_MOUSE
AccessioniPrimary (citable) accession number: A2A5N8
Secondary accession number(s): Q5DU20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: February 20, 2007
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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