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Protein

Post-GPI attachment to proteins factor 3

Gene

Pgap3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processGPI-anchor biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Post-GPI attachment to proteins factor 3
Alternative name(s):
PER1-like domain-containing protein 1
Gene namesi
Name:Pgap3
Synonyms:Perld1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2444461. Pgap3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 98LumenalSequence analysisAdd BLAST75
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Topological domaini120 – 135CytoplasmicSequence analysisAdd BLAST16
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Topological domaini157 – 169LumenalSequence analysisAdd BLAST13
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 198CytoplasmicSequence analysis8
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Topological domaini220 – 223LumenalSequence analysis4
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21
Topological domaini245 – 257CytoplasmicSequence analysisAdd BLAST13
Transmembranei258 – 278HelicalSequence analysisAdd BLAST21
Topological domaini279LumenalSequence analysis1
Transmembranei280 – 299HelicalSequence analysisAdd BLAST20
Topological domaini300 – 320CytoplasmicSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000033935724 – 320Post-GPI attachment to proteins factor 3Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiA2A559.
PaxDbiA2A559.
PRIDEiA2A559.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038208.
GenevisibleiA2A559. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000088337.

Family & Domainsi

Sequence similaritiesi

Belongs to the PGAP3 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2970. Eukaryota.
COG5237. LUCA.
GeneTreeiENSGT00390000001304.
HOGENOMiHOG000210016.
HOVERGENiHBG108243.
InParanoidiA2A559.
OMAiHTRDFPL.
OrthoDBiEOG091G0ARL.
PhylomeDBiA2A559.
TreeFamiTF300031.

Family and domain databases

InterProiView protein in InterPro
IPR007217. Per1-like.
PANTHERiPTHR13148. PTHR13148. 1 hit.
PfamiView protein in Pfam
PF04080. Per1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2A559-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKRTAPLLL LTLAVGLAGG SQGDREPVYR DCVLRCEERN CSGDALKHFR
60 70 80 90 100
SRQPIYMSLA GWTCRDDCKY ECMWFTVGLY LQEGHRVPQF HGKWPFSRFL
110 120 130 140 150
FIQEPASAVA SLLNGLASLV MLCRYRASVP ASSPMYHTCM AFAWVSLNAW
160 170 180 190 200
FWSTVFHTRD TDLTEKMDYF CASAVILHSV YLCCVRTVGL QHPSVASAFG
210 220 230 240 250
ALLLLLLTGH ISYLSLVHFD YGYNMMANVA IGLVNLAWWL VWCLRNRQRL
260 270 280 290 300
PHTRRCMVVV VLLQGLSLLE LLDFPPLFWV LDAHAIWHIS TIPVHTLFFR
310 320
FLEDDSLYLL KESGAMFKLD
Length:320
Mass (Da):36,528
Last modified:February 20, 2007 - v1
Checksum:i151EA9AB17E95BDC
GO
Isoform 2 (identifier: A2A559-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-144: Missing.

Note: No experimental confirmation available.
Show »
Length:269
Mass (Da):30,849
Checksum:i9B0920BB5AD5958C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277L → P in BAE42049 (PubMed:16141072).Curated1
Sequence conflicti281L → P in BAE42049 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03415994 – 144Missing in isoform 2. CuratedAdd BLAST51

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK170817 mRNA. Translation: BAE42049.1.
AL591390 Genomic DNA. Translation: CAM22011.1.
AL591390 Genomic DNA. Translation: CAM22012.1.
CCDSiCCDS25348.1. [A2A559-1]
RefSeqiNP_001028709.2. NM_001033537.2. [A2A559-1]
XP_006533612.1. XM_006533549.3. [A2A559-2]
UniGeneiMm.86519.

Genome annotation databases

EnsembliENSMUST00000090827; ENSMUSP00000088337; ENSMUSG00000038208. [A2A559-1]
ENSMUST00000128897; ENSMUSP00000119668; ENSMUSG00000038208. [A2A559-2]
GeneIDi320655.
KEGGimmu:320655.
UCSCiuc007lgh.1. mouse. [A2A559-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPGAP3_MOUSE
AccessioniPrimary (citable) accession number: A2A559
Secondary accession number(s): A2A558, Q3TCA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: February 20, 2007
Last modified: June 7, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families