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Protein

ATP-dependent RNA helicase DHX8

Gene

Dhx8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi612 – 6198ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHX8 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 8
Gene namesi
Name:Dhx8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1306823. Dhx8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12441244ATP-dependent RNA helicase DHX8PRO_0000330831Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei419 – 4191PhosphoserineBy similarity
Modified residuei484 – 4841PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2A4P0.
MaxQBiA2A4P0.
PaxDbiA2A4P0.
PeptideAtlasiA2A4P0.
PRIDEiA2A4P0.

PTM databases

iPTMnetiA2A4P0.

Expressioni

Gene expression databases

BgeeiA2A4P0.
ExpressionAtlasiA2A4P0. baseline and differential.
GenevisibleiA2A4P0. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037251.

Structurei

3D structure databases

ProteinModelPortaliA2A4P0.
SMRiA2A4P0. Positions 284-379, 974-1209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini289 – 36072S1 motifPROSITE-ProRule annotationAdd
BLAST
Domaini599 – 762164Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini780 – 960181Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi709 – 7124DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi163 – 274112Arg-richAdd
BLAST
Compositional biasi176 – 25277Arg/Ser-rich (RS domain)Add
BLAST

Domaini

The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.By similarity

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410INHE. Eukaryota.
KOG0922. Eukaryota.
KOG0925. Eukaryota.
COG1185. LUCA.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOVERGENiHBG039428.
InParanoidiA2A4P0.
KOiK12818.
OMAiAMMELEA.
OrthoDBiEOG7FXZXR.
TreeFamiTF300509.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2A4P0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAVAAAGV LMGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA
60 70 80 90 100
EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST
110 120 130 140 150
SKDPVVKPKT EKEKLRELFP VLCQPDNPSA RTMLDEEDVK VAVDVLKELE
160 170 180 190 200
ALMPSAAGQE KQRDPEHRDR TKKKKRSRSR DRDRDRDRDR DRDRDRDRDR
210 220 230 240 250
DKDRERDRDR ERDRERDRER DHKRRHRSRS RSHSRTRERT KGKSRYRSRS
260 270 280 290 300
RSQSPFKDRK DREKYGERNL DRWRDKHVDR PPPEEPAIGD IYNGKVTSIM
310 320 330 340 350
QFGCFVQLEG LRKRWEGLVH ISELRREGRV ANVADVVSKG QRVKVKVLSF
360 370 380 390 400
TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV
410 420 430 440 450
SAPEVEDDSL ERKRLTRISD PEKWEIKQMI AANVLSKEEF PDFDEETGIL
460 470 480 490 500
PKVDDEEDED LEIELVEEEP PFLRGHTKQS MDMSPIKIVK NPDGSLSQAA
510 520 530 540 550
MMQSALAKER RELKQAQREA EMDSIPMGLN KHWVDPLPDA EGRQIAANMR
560 570 580 590 600
GIGMMPNDIP EWKKHAFGGN KASYGKKTQM SILEQRESLP IYKLKEQLVQ
610 620 630 640 650
AVHDNQILIV IGETGSGKTT QITQYLAEAG YTSRGKIGCT QPRRVAAMSV
660 670 680 690 700
AKRVSEEFGC CLGQEVGYTI RFEDCTSPET VIKYMTDGML LRECLIDPDL
710 720 730 740 750
TQYAIIMLDE AHERTIHTDV LFGLLKKTVQ KRQDMKLIVT SATLDAVKFS
760 770 780 790 800
QYFYEAPIFT IPGRTYPVEI LYTKEPETDY LDASLITVMQ IHLTEPPGDI
810 820 830 840 850
LVFLTGQEEI DTACEILYER MKSLGPDVPE LIILPVYSAL PSEMQTRIFD
860 870 880 890 900
PAPPGSRKVV IATNIAETSL TIDGIYYVVD PGFVKQKVYN SKTGIDQLVV
910 920 930 940 950
TPISQAQAKQ RAGRAGRTGP GKCYRLYTER AYRDEMLTTN VPEIQRTNLA
960 970 980 990 1000
STVLSLKAMG INDLLSFDFM DAPPMETLIT AMEQLYTLGA LDDEGLLTRL
1010 1020 1030 1040 1050
GRRMAEFPLE PMLCKMLIMS VHLGCSEEML TIVSMLSVQN VFYRPKDKQA
1060 1070 1080 1090 1100
LADQKKAKFH QTEGDHLTLL AVYNSWKNNK FSNPWCYENF IQARSLRRAQ
1110 1120 1130 1140 1150
DIRKQMLGIM DRHKLDVVSC GKSTVRVQKA ICSGFFRNAA KKDPQEGYRT
1160 1170 1180 1190 1200
LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF
1210 1220 1230 1240
APAFFKVSDP TKLSKQKKQQ RLEPLYNRYE EPNAWRISRA FRRR
Length:1,244
Mass (Da):142,572
Last modified:February 20, 2007 - v1
Checksum:i8FD07949CCCB4DD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC132445 mRNA. Translation: AAI32446.1.
AL590994, AL591436 Genomic DNA. Translation: CAM22647.1.
AL591436, AL590994 Genomic DNA. Translation: CAM24250.1.
CCDSiCCDS25479.1.
RefSeqiNP_659080.2. NM_144831.2.
UniGeneiMm.28186.

Genome annotation databases

EnsembliENSMUST00000039152; ENSMUSP00000037251; ENSMUSG00000034931.
GeneIDi217207.
KEGGimmu:217207.
UCSCiuc007lpr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC132445 mRNA. Translation: AAI32446.1.
AL590994, AL591436 Genomic DNA. Translation: CAM22647.1.
AL591436, AL590994 Genomic DNA. Translation: CAM24250.1.
CCDSiCCDS25479.1.
RefSeqiNP_659080.2. NM_144831.2.
UniGeneiMm.28186.

3D structure databases

ProteinModelPortaliA2A4P0.
SMRiA2A4P0. Positions 284-379, 974-1209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037251.

PTM databases

iPTMnetiA2A4P0.

Proteomic databases

EPDiA2A4P0.
MaxQBiA2A4P0.
PaxDbiA2A4P0.
PeptideAtlasiA2A4P0.
PRIDEiA2A4P0.

Protocols and materials databases

DNASUi217207.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039152; ENSMUSP00000037251; ENSMUSG00000034931.
GeneIDi217207.
KEGGimmu:217207.
UCSCiuc007lpr.1. mouse.

Organism-specific databases

CTDi1659.
MGIiMGI:1306823. Dhx8.

Phylogenomic databases

eggNOGiENOG410INHE. Eukaryota.
KOG0922. Eukaryota.
KOG0925. Eukaryota.
COG1185. LUCA.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOVERGENiHBG039428.
InParanoidiA2A4P0.
KOiK12818.
OMAiAMMELEA.
OrthoDBiEOG7FXZXR.
TreeFamiTF300509.

Miscellaneous databases

PROiA2A4P0.
SOURCEiSearch...

Gene expression databases

BgeeiA2A4P0.
ExpressionAtlasiA2A4P0. baseline and differential.
GenevisibleiA2A4P0. MM.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiDHX8_MOUSE
AccessioniPrimary (citable) accession number: A2A4P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 20, 2007
Last modified: July 6, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.