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Protein

ATP-dependent RNA helicase DHX8

Gene

Dhx8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi612 – 619ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHX8 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 8
Gene namesi
Name:Dhx8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1306823. Dhx8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003308311 – 1244ATP-dependent RNA helicase DHX8Add BLAST1244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei419PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2A4P0.
MaxQBiA2A4P0.
PaxDbiA2A4P0.
PeptideAtlasiA2A4P0.
PRIDEiA2A4P0.

PTM databases

iPTMnetiA2A4P0.
PhosphoSitePlusiA2A4P0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034931.
ExpressionAtlasiA2A4P0. baseline and differential.
GenevisibleiA2A4P0. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037251.

Structurei

3D structure databases

ProteinModelPortaliA2A4P0.
SMRiA2A4P0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini289 – 360S1 motifPROSITE-ProRule annotationAdd BLAST72
Domaini599 – 762Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST164
Domaini780 – 960Helicase C-terminalPROSITE-ProRule annotationAdd BLAST181

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi709 – 712DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi163 – 274Arg-richAdd BLAST112
Compositional biasi176 – 252Arg/Ser-rich (RS domain)Add BLAST77

Domaini

The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.By similarity

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410INHE. Eukaryota.
KOG0922. Eukaryota.
KOG0925. Eukaryota.
COG1185. LUCA.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOVERGENiHBG039428.
InParanoidiA2A4P0.
KOiK12818.
OMAiERWRDKH.
OrthoDBiEOG091G030Q.
TreeFamiTF300509.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2A4P0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAVAAAGV LMGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA
60 70 80 90 100
EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST
110 120 130 140 150
SKDPVVKPKT EKEKLRELFP VLCQPDNPSA RTMLDEEDVK VAVDVLKELE
160 170 180 190 200
ALMPSAAGQE KQRDPEHRDR TKKKKRSRSR DRDRDRDRDR DRDRDRDRDR
210 220 230 240 250
DKDRERDRDR ERDRERDRER DHKRRHRSRS RSHSRTRERT KGKSRYRSRS
260 270 280 290 300
RSQSPFKDRK DREKYGERNL DRWRDKHVDR PPPEEPAIGD IYNGKVTSIM
310 320 330 340 350
QFGCFVQLEG LRKRWEGLVH ISELRREGRV ANVADVVSKG QRVKVKVLSF
360 370 380 390 400
TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV
410 420 430 440 450
SAPEVEDDSL ERKRLTRISD PEKWEIKQMI AANVLSKEEF PDFDEETGIL
460 470 480 490 500
PKVDDEEDED LEIELVEEEP PFLRGHTKQS MDMSPIKIVK NPDGSLSQAA
510 520 530 540 550
MMQSALAKER RELKQAQREA EMDSIPMGLN KHWVDPLPDA EGRQIAANMR
560 570 580 590 600
GIGMMPNDIP EWKKHAFGGN KASYGKKTQM SILEQRESLP IYKLKEQLVQ
610 620 630 640 650
AVHDNQILIV IGETGSGKTT QITQYLAEAG YTSRGKIGCT QPRRVAAMSV
660 670 680 690 700
AKRVSEEFGC CLGQEVGYTI RFEDCTSPET VIKYMTDGML LRECLIDPDL
710 720 730 740 750
TQYAIIMLDE AHERTIHTDV LFGLLKKTVQ KRQDMKLIVT SATLDAVKFS
760 770 780 790 800
QYFYEAPIFT IPGRTYPVEI LYTKEPETDY LDASLITVMQ IHLTEPPGDI
810 820 830 840 850
LVFLTGQEEI DTACEILYER MKSLGPDVPE LIILPVYSAL PSEMQTRIFD
860 870 880 890 900
PAPPGSRKVV IATNIAETSL TIDGIYYVVD PGFVKQKVYN SKTGIDQLVV
910 920 930 940 950
TPISQAQAKQ RAGRAGRTGP GKCYRLYTER AYRDEMLTTN VPEIQRTNLA
960 970 980 990 1000
STVLSLKAMG INDLLSFDFM DAPPMETLIT AMEQLYTLGA LDDEGLLTRL
1010 1020 1030 1040 1050
GRRMAEFPLE PMLCKMLIMS VHLGCSEEML TIVSMLSVQN VFYRPKDKQA
1060 1070 1080 1090 1100
LADQKKAKFH QTEGDHLTLL AVYNSWKNNK FSNPWCYENF IQARSLRRAQ
1110 1120 1130 1140 1150
DIRKQMLGIM DRHKLDVVSC GKSTVRVQKA ICSGFFRNAA KKDPQEGYRT
1160 1170 1180 1190 1200
LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF
1210 1220 1230 1240
APAFFKVSDP TKLSKQKKQQ RLEPLYNRYE EPNAWRISRA FRRR
Length:1,244
Mass (Da):142,572
Last modified:February 20, 2007 - v1
Checksum:i8FD07949CCCB4DD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC132445 mRNA. Translation: AAI32446.1.
AL590994, AL591436 Genomic DNA. Translation: CAM22647.1.
AL591436, AL590994 Genomic DNA. Translation: CAM24250.1.
CCDSiCCDS25479.1.
RefSeqiNP_659080.2. NM_144831.2.
UniGeneiMm.28186.

Genome annotation databases

EnsembliENSMUST00000039152; ENSMUSP00000037251; ENSMUSG00000034931.
GeneIDi217207.
KEGGimmu:217207.
UCSCiuc007lpr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC132445 mRNA. Translation: AAI32446.1.
AL590994, AL591436 Genomic DNA. Translation: CAM22647.1.
AL591436, AL590994 Genomic DNA. Translation: CAM24250.1.
CCDSiCCDS25479.1.
RefSeqiNP_659080.2. NM_144831.2.
UniGeneiMm.28186.

3D structure databases

ProteinModelPortaliA2A4P0.
SMRiA2A4P0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037251.

PTM databases

iPTMnetiA2A4P0.
PhosphoSitePlusiA2A4P0.

Proteomic databases

EPDiA2A4P0.
MaxQBiA2A4P0.
PaxDbiA2A4P0.
PeptideAtlasiA2A4P0.
PRIDEiA2A4P0.

Protocols and materials databases

DNASUi217207.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039152; ENSMUSP00000037251; ENSMUSG00000034931.
GeneIDi217207.
KEGGimmu:217207.
UCSCiuc007lpr.1. mouse.

Organism-specific databases

CTDi1659.
MGIiMGI:1306823. Dhx8.

Phylogenomic databases

eggNOGiENOG410INHE. Eukaryota.
KOG0922. Eukaryota.
KOG0925. Eukaryota.
COG1185. LUCA.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOVERGENiHBG039428.
InParanoidiA2A4P0.
KOiK12818.
OMAiERWRDKH.
OrthoDBiEOG091G030Q.
TreeFamiTF300509.

Miscellaneous databases

PROiA2A4P0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034931.
ExpressionAtlasiA2A4P0. baseline and differential.
GenevisibleiA2A4P0. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHX8_MOUSE
AccessioniPrimary (citable) accession number: A2A4P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.