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Protein

Cullin-4B

Gene

Cul4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-MMU-5696394. DNA Damage Recognition in GG-NER.
R-MMU-5696395. Formation of Incision Complex in GG-NER.
R-MMU-5696400. Dual Incision in GG-NER.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.
R-MMU-6782135. Dual incision in TC-NER.
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-4BImported
Short name:
CUL-4BBy similarity
Gene namesi
Name:Cul4bImported
Synonyms:mKIAA0695Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1919834. Cul4b.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003944261 – 970Cullin-4BAdd BLAST970

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphothreonineCombined sources1
Modified residuei17PhosphoserineCombined sources1
Modified residuei106PhosphothreonineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Modified residuei154PhosphoserineBy similarity1
Modified residuei200PhosphoserineBy similarity1
Modified residuei202PhosphothreonineCombined sources1
Cross-linki247Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei250PhosphoserineBy similarity1
Cross-linki916Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiA2A432.
MaxQBiA2A432.
PaxDbiA2A432.
PeptideAtlasiA2A432.
PRIDEiA2A432.

PTM databases

iPTMnetiA2A432.
PhosphoSitePlusiA2A432.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031095.
ExpressionAtlasiA2A432. baseline and differential.
GenevisibleiA2A432. MM.

Interactioni

Subunit structurei

Component of multiple DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes that seem to be formed of DDB1, CUL4A or CUL4B, RBX1 and a variable substrate recognition component which seems to belong to a protein family described as DCAF (Ddb1- and Cul4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Component of the DCX(DTL) complex with the putative substrate recognition component DTL. Component of the DCX(DDB2) complex with the putative substrate recognition component DDB2. Part of a complex with RBX1 and TIP120A/CAND1. Interacts with RBX1 GRWD1, MLST8, SMU1, TLE2, TLE3, VPRBP, DDA1, DCAF6, DCAF17, DDB2, DCAF8, TIP120A/CAND1 and TMEM113. Interacts with cyclin E and with importins alpha-1 (KPNA2), alpha-3 (KPNA4), alpha-5 (KPNA1) and beta-1 (KPNB1). May interact with WDR26, WDR51B, SNRNP40, WDR61, WDR76 and WDR5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215452. 2 interactors.
IntActiA2A432. 2 interactors.
STRINGi10090.ENSMUSP00000059276.

Structurei

3D structure databases

ProteinModelPortaliA2A432.
SMRiA2A432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi112 – 115Nuclear localization signalBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 56Pro-richSequence analysisAdd BLAST21
Compositional biasi118 – 250Ser-richSequence analysisAdd BLAST133

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2167. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOVERGENiHBG003619.
InParanoidiA2A432.
KOiK10609.
OMAiLVTVRWI.
OrthoDBiEOG091G02DP.
PhylomeDBiA2A432.
TreeFamiTF101153.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR033044. CUL4B.
IPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11932:SF66. PTHR11932:SF66. 1 hit.
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 14 Publications (identifier: A2A432-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRSTRSKER RENDTDSEDN SSETSNQERR RCRQGPPRPP YPPLLPPVFP
60 70 80 90 100
PPTPPPQVRR TRGLQDLGAM KSVCPGTSGF SSPNPSAASA AAQEVRSATD
110 120 130 140 150
GNTSTTPPTS AKKRKLNSSS SSSNSSNERE DFDSTSSSST PPQPRDSASP
160 170 180 190 200
STSSFCLGVP VATSSHVPIQ KKLRFEDTLE FVGIDTKMAE ESSSSSSSSS
210 220 230 240 250
PTAATSQQQQ QQQLKTKSIL ISSVASVHHA NGLAKSSTAV SSFANSKPGS
260 270 280 290 300
AKKLVIKNFK DKPKLPENYT DETWQKLKEA VEAIQNSTSI KYNLEELYQA
310 320 330 340 350
VENLCSHKIS ANLYKQLRQI CEDHIKAQIH QFREDSLDSV LFLKKIDRCW
360 370 380 390 400
QNHCRQMIMI RSIFLFLDRT YVLQNSMLPS IWDMGLELFR AHIISDQKVQ
410 420 430 440 450
TKTIDGILLL IERERNGEAI DRSLLRSLLS MLSDLQIYQD SFEQQFLQET
460 470 480 490 500
NRLYAAEGQK LMQEREVPEY LHHVNKRLEE EADRLITYLD QTTQKSLIAS
510 520 530 540 550
VEKQLLGEHL TAILQKGLNS LLDENRIQDL SLLYQLFSRV RGGVQVLLQQ
560 570 580 590 600
WIEYIKAFGS TIVINPEKDK TMVQELLDFK DKVDHIIDTC FLKNEKFINA
610 620 630 640 650
MKEAFETFIN KRPNKPAELI AKYVDSKLRA GNKEATDEEL EKMLDKIMII
660 670 680 690 700
FRFIYGKDVF EAFYKKDLAK RLLVGKSASV DAEKSMLSKL KHECGAAFTS
710 720 730 740 750
KLEGMFKDME LSKDIMIQFK QYMQNQNVPG NIELTVNILT MGYWPTYVPM
760 770 780 790 800
EVHLPPEMVK LQEIFKTFYL GKHSGRKLQW QSTLGHCVLK AEFKEGKKEL
810 820 830 840 850
QVSLFQTMVL LMFNEGEEFS LEEIKHATGI EDGELRRTLQ SLACGKARVL
860 870 880 890 900
AKNPKGKDIE DGDKFICNDD FKHKLFRIKI NQIQMKETVE EQASTTERVF
910 920 930 940 950
QDRQYQIDAA IVRIMKMRKT LSHNLLVSEV YNQLKFPVKP ADLKKRIESL
960 970
IDRDYMERDK ENPNQYNYIA
Length:970
Mass (Da):110,699
Last modified:February 20, 2007 - v1
Checksum:i87D518B268A21849
GO
Isoform 21 Publication (identifier: A2A432-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     693-721: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:941
Mass (Da):107,391
Checksum:iD829FE646A1D7574
GO

Sequence cautioni

The sequence AAH04026 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH10347 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC41443 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56P → A in BAB28222 (PubMed:16141072).Curated1
Sequence conflicti209Q → E in BAC41443 (PubMed:12465718).Curated1
Sequence conflicti593K → E in BAB28222 (PubMed:16141072).Curated1
Sequence conflicti933Q → R in AAP84984 (PubMed:16052071).Curated1
Sequence conflicti933Q → R in BAC27992 (PubMed:16141072).Curated1
Sequence conflicti966Y → S in BAC41443 (PubMed:12465718).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039242693 – 721Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330868 mRNA. Translation: AAP84984.1.
AK012410 mRNA. Translation: BAB28222.2.
AK032701 mRNA. Translation: BAC27992.1.
AK160998 mRNA. Translation: BAE36141.1.
AL513356 Genomic DNA. Translation: CAM17145.1.
CH466570 Genomic DNA. Translation: EDL29019.1.
BC004026 mRNA. Translation: AAH04026.1. Different initiation.
BC010347 mRNA. Translation: AAH10347.1. Different initiation.
AB093259 Transcribed RNA. Translation: BAC41443.3. Different initiation.
CCDSiCCDS40948.1. [A2A432-1]
RefSeqiNP_001103612.1. NM_001110142.1. [A2A432-1]
NP_082564.3. NM_028288.5. [A2A432-1]
UniGeneiMm.327675.

Genome annotation databases

EnsembliENSMUST00000050083; ENSMUSP00000059276; ENSMUSG00000031095. [A2A432-1]
ENSMUST00000115118; ENSMUSP00000110771; ENSMUSG00000031095. [A2A432-1]
GeneIDi72584.
KEGGimmu:72584.
UCSCiuc009taa.2. mouse. [A2A432-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330868 mRNA. Translation: AAP84984.1.
AK012410 mRNA. Translation: BAB28222.2.
AK032701 mRNA. Translation: BAC27992.1.
AK160998 mRNA. Translation: BAE36141.1.
AL513356 Genomic DNA. Translation: CAM17145.1.
CH466570 Genomic DNA. Translation: EDL29019.1.
BC004026 mRNA. Translation: AAH04026.1. Different initiation.
BC010347 mRNA. Translation: AAH10347.1. Different initiation.
AB093259 Transcribed RNA. Translation: BAC41443.3. Different initiation.
CCDSiCCDS40948.1. [A2A432-1]
RefSeqiNP_001103612.1. NM_001110142.1. [A2A432-1]
NP_082564.3. NM_028288.5. [A2A432-1]
UniGeneiMm.327675.

3D structure databases

ProteinModelPortaliA2A432.
SMRiA2A432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215452. 2 interactors.
IntActiA2A432. 2 interactors.
STRINGi10090.ENSMUSP00000059276.

PTM databases

iPTMnetiA2A432.
PhosphoSitePlusiA2A432.

Proteomic databases

EPDiA2A432.
MaxQBiA2A432.
PaxDbiA2A432.
PeptideAtlasiA2A432.
PRIDEiA2A432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050083; ENSMUSP00000059276; ENSMUSG00000031095. [A2A432-1]
ENSMUST00000115118; ENSMUSP00000110771; ENSMUSG00000031095. [A2A432-1]
GeneIDi72584.
KEGGimmu:72584.
UCSCiuc009taa.2. mouse. [A2A432-1]

Organism-specific databases

CTDi8450.
MGIiMGI:1919834. Cul4b.

Phylogenomic databases

eggNOGiKOG2167. Eukaryota.
COG5647. LUCA.
GeneTreeiENSGT00760000119212.
HOVERGENiHBG003619.
InParanoidiA2A432.
KOiK10609.
OMAiLVTVRWI.
OrthoDBiEOG091G02DP.
PhylomeDBiA2A432.
TreeFamiTF101153.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-MMU-5696394. DNA Damage Recognition in GG-NER.
R-MMU-5696395. Formation of Incision Complex in GG-NER.
R-MMU-5696400. Dual Incision in GG-NER.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.
R-MMU-6782135. Dual incision in TC-NER.
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Miscellaneous databases

ChiTaRSiCul4b. mouse.
PROiA2A432.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031095.
ExpressionAtlasiA2A432. baseline and differential.
GenevisibleiA2A432. MM.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR033044. CUL4B.
IPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11932:SF66. PTHR11932:SF66. 1 hit.
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUL4B_MOUSE
AccessioniPrimary (citable) accession number: A2A432
Secondary accession number(s): Q3TU30
, Q8BSL3, Q8CHD6, Q91YZ7, Q99KS9, Q9CZM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.