ID   A2A114_NEOCA            Unreviewed;       564 AA.
AC   A2A114;
DT   20-FEB-2007, integrated into UniProtKB/TrEMBL.
DT   20-FEB-2007, sequence version 1.
DT   22-FEB-2023, entry version 37.
DE   SubName: Full=Apical membrane antigen 1 {ECO:0000313|EMBL:BAF45372.1};
GN   Name=AMA1 {ECO:0000313|EMBL:BAF45372.1};
OS   Neospora caninum (Coccidian parasite).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Sarcocystidae; Neospora.
OX   NCBI_TaxID=29176 {ECO:0000313|EMBL:BAF45372.1};
RN   [1] {ECO:0000313|EMBL:BAF45372.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=NC-1 {ECO:0000313|EMBL:BAF45372.1};
RX   PubMed=17156863; DOI=10.1016/j.molbiopara.2006.11.005;
RA   Zhang H., Compaore M.K., Lee EG., Liao M., Zhang G., Sugimoto C.,
RA   Fujisaki K., Nishikawa Y., Xuan X.;
RT   "Apical membrane antigen 1 is a cross-reactive antigen between Neospora
RT   caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host
RT   cell invasion by both parasites.";
RL   Mol. Biochem. Parasitol. 151:205-212(2007).
RN   [2] {ECO:0007829|PDB:4APL}
RP   X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 59-478, AND DISULFIDE BONDS.
RX   PubMed=23169033; DOI=10.1002/pro.2193;
RA   Tonkin M.L., Crawford J., Lebrun M.L., Boulanger M.J.;
RT   "Babesia divergens and Neospora caninum apical membrane antigen 1
RT   structures reveal selectivity and plasticity in apicomplexan parasite host
RT   cell invasion.";
RL   Protein Sci. 22:114-127(2013).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004479}; Single-
CC       pass type I membrane protein {ECO:0000256|ARBA:ARBA00004479}.
CC   -!- SIMILARITY: Belongs to the apicomplexan parasites AMA1 family.
CC       {ECO:0000256|ARBA:ARBA00007098}.
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DR   EMBL; AB265823; BAF45372.1; -; mRNA.
DR   PDB; 4APL; X-ray; 2.90 A; A/B/D/E=59-478.
DR   PDBsum; 4APL; -.
DR   AlphaFoldDB; A2A114; -.
DR   SMR; A2A114; -.
DR   VEuPathDB; ToxoDB:NCLIV_028680; -.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.10.70.70; -; 1.
DR   Gene3D; 3.50.4.10; Hepatocyte Growth Factor; 2.
DR   InterPro; IPR003298; Apmem_Ag1.
DR   Pfam; PF02430; AMA-1; 1.
DR   PRINTS; PR01361; MEROZOITESA.
DR   SMART; SM00815; AMA-1; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4APL}; Membrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        21..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        478..501
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          47..66
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          522..541
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        111..280
FT                   /evidence="ECO:0007829|PDB:4APL"
FT   DISULFID        188..220
FT                   /evidence="ECO:0007829|PDB:4APL"
FT   DISULFID        236..249
FT                   /evidence="ECO:0007829|PDB:4APL"
FT   DISULFID        298..387
FT                   /evidence="ECO:0007829|PDB:4APL"
FT   DISULFID        318..378
FT                   /evidence="ECO:0007829|PDB:4APL"
FT   DISULFID        429..453
FT                   /evidence="ECO:0007829|PDB:4APL"
FT   DISULFID        441..465
FT                   /evidence="ECO:0007829|PDB:4APL"
FT   DISULFID        446..473
FT                   /evidence="ECO:0007829|PDB:4APL"
SQ   SEQUENCE   564 AA;  62813 MW;  F2E5FA8F903E0F9E CRC64;
     MGSWRRERPA RPSDQQWITE WRQIGIVGVA ILLVLVAESS LLATGINPKT RSGGSQTLSA
     STPQNPFQTP ELKAFLERYN VTLHHQSGVN VDLGQDKEVD GKLYREAAGL CPVWGKYIQL
     HQPNRPPYKN NFLEDIPTEA EYQKSGNPLP GGFNMNFVTP AGQRISPYPM ELLEKSSKIK
     ASTELGKCAE FAYKTTAMDK SNQATQYRYP FVYDSKRRLC YILSVSMQRM EGSKYCSTND
     DPVNLTWYCF EPQKSPTANH NLIFGSAYVG KDPDAFLTKC PNQALKGYRF GHWTNGRCHD
     YTELADSWIE AVDSKAQCWV KTFTNDEVAS DQPRTYPLTS QHSWNDWWPV HEKDQPHSGG
     DGRNYGFFYF DSNGKGKCAL SHKAPDCLVS DSKAVSYTAL GSLSEETPDI IIPSNPSVNP
     STPDEALQCR SSEFPETFGS CDVQACKRQK TSCVGGQIQS TSVDCTAEEQ NDCGSNTALI
     AGMAIGGVLI MGLLAGSGYY YSKRLNTNQG VPAVDHDHEF QTQRNAQKKR PSDLMQEAEP
     SFWDEAEENI DQSGETQVLV EGDY
//