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A1ZAW5

- ICLN_DROME

UniProt

A1ZAW5 - ICLN_DROME

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Protein
Methylosome subunit pICln
Gene
icln, CG4924
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus By similarity.1 Publication

GO - Biological processi

  1. cell volume homeostasis Source: InterPro
  2. chloride transport Source: InterPro
  3. spliceosomal snRNP assembly Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome subunit pICln
Gene namesi
Name:icln
ORF Names:CG4924
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0029079. icln.

Subcellular locationi

Cytoplasmcytosol By similarity. Nucleus By similarity. Cytoplasmcytoskeleton By similarity

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-SubCell
  2. cytosol Source: UniProtKB-SubCell
  3. methylosome Source: InterPro
  4. nucleus Source: UniProtKB-SubCell
  5. pICln-Sm protein complex Source: InterPro
  6. plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Methylosome subunit pICln
PRO_0000425412Add
BLAST

Proteomic databases

PaxDbiA1ZAW5.
PRIDEiA1ZAW5.

Expressioni

Gene expression databases

BgeeiA1ZAW5.

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least CLNS1A/pICln, PRMT5/SKB1 and WDR77/MEP50; may mediate SNRPD1 and SNRPD3 methylation. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP.1 Publication

Protein-protein interaction databases

IntActiA1ZAW5. 1 interaction.
STRINGi7227.FBpp0086010.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54
Beta strandi14 – 2714
Beta strandi29 – 5022
Beta strandi52 – 565
Helixi57 – 593
Beta strandi60 – 7920
Turni84 – 863
Beta strandi133 – 1419
Helixi145 – 15915

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VU2X-ray3.103/G/O/W/e/m/u1-180[»]
1VU3X-ray3.10G/O/W/e/m/u1-180[»]
4F77X-ray3.103/G/O/W/e/m/u1-180[»]
4F7UX-ray1.90P/Q1-160[»]
ProteinModelPortaliA1ZAW5.
SMRiA1ZAW5. Positions 1-168.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG290633.
GeneTreeiENSGT00390000010063.
InParanoidiA1ZAW5.
KOiK05019.
OMAiYTANNIK.
OrthoDBiEOG7H1JMG.
PhylomeDBiA1ZAW5.

Family and domain databases

InterProiIPR003521. ICln.
[Graphical view]
PANTHERiPTHR21399. PTHR21399. 1 hit.
PRINTSiPR01348. ICLNCHANNEL.

Sequencei

Sequence statusi: Complete.

A1ZAW5-1 [UniParc]FASTAAdd to Basket

« Hide

MVLIMRVSPP EHGLLYTANN IKLKLGDKVV GEGTVYIAQN TLSWQPTELA    50
EGISIEWKQV SLHGISSNPR KCIYFMLDHK VEWNGVYGDP PQQAVNGRNG 100
GGSEAEVDEG NGSDEHDEDD NFEDAVDEQF GEVTECWLMP EDIHTVDTMY 150
SAMTTCQALH PDSANSDSED SDPMQDAGGL EDEAMEEDDA LTLGRNGVQN 200
LSLDDDEERF EDADE 215
Length:215
Mass (Da):23,743
Last modified:February 6, 2007 - v1
Checksum:iAD92706F7891D800
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61R → H in AAF21126. 1 Publication
Sequence conflicti6 – 61R → H in AAL89952. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF216522 mRNA. Translation: AAF21126.1.
AE013599 Genomic DNA. Translation: AAF57826.1.
AY084214 mRNA. Translation: AAL89952.1.
RefSeqiNP_611237.2. NM_137393.4.
UniGeneiDm.6477.

Genome annotation databases

EnsemblMetazoaiFBtr0086832; FBpp0086010; FBgn0029079.
GeneIDi36997.
KEGGidme:Dmel_CG4924.
UCSCiCG4924-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF216522 mRNA. Translation: AAF21126.1 .
AE013599 Genomic DNA. Translation: AAF57826.1 .
AY084214 mRNA. Translation: AAL89952.1 .
RefSeqi NP_611237.2. NM_137393.4.
UniGenei Dm.6477.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VU2 X-ray 3.10 3/G/O/W/e/m/u 1-180 [» ]
1VU3 X-ray 3.10 G/O/W/e/m/u 1-180 [» ]
4F77 X-ray 3.10 3/G/O/W/e/m/u 1-180 [» ]
4F7U X-ray 1.90 P/Q 1-160 [» ]
ProteinModelPortali A1ZAW5.
SMRi A1ZAW5. Positions 1-168.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi A1ZAW5. 1 interaction.
STRINGi 7227.FBpp0086010.

Proteomic databases

PaxDbi A1ZAW5.
PRIDEi A1ZAW5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0086832 ; FBpp0086010 ; FBgn0029079 .
GeneIDi 36997.
KEGGi dme:Dmel_CG4924.
UCSCi CG4924-RA. d. melanogaster.

Organism-specific databases

CTDi 36997.
FlyBasei FBgn0029079. icln.

Phylogenomic databases

eggNOGi NOG290633.
GeneTreei ENSGT00390000010063.
InParanoidi A1ZAW5.
KOi K05019.
OMAi YTANNIK.
OrthoDBi EOG7H1JMG.
PhylomeDBi A1ZAW5.

Miscellaneous databases

GenomeRNAii 36997.
NextBioi 801424.
PROi A1ZAW5.

Gene expression databases

Bgeei A1ZAW5.

Family and domain databases

InterProi IPR003521. ICln.
[Graphical view ]
PANTHERi PTHR21399. PTHR21399. 1 hit.
PRINTSi PR01348. ICLNCHANNEL.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of ICLn."
    Laurencon A., Hawley S.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Structural basis of assembly chaperone-mediated snRNP formation."
    Grimm C., Chari A., Pelz J.P., Kuper J., Kisker C., Diederichs K., Stark H., Schindelin H., Fischer U.
    Mol. Cell 49:692-703(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-180 IN 6S PICLN-SM COMPLEX, IDENTIFICATION IN 6S PICLN-SM COMPLEX, FUNCTION IN SNRNP BIOGENESIS.

Entry informationi

Entry nameiICLN_DROME
AccessioniPrimary (citable) accession number: A1ZAW5
Secondary accession number(s): Q9U3W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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