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Protein

PDZ and LIM domain protein Zasp

Gene

Zasp52

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of cell matrix adhesion having two related functions, one upstream of Actn organizing the Z line and the other downstream of integrins regulating assembly of integrin adhesion sites. Also required for the formation of myotendinous junctions in muscles.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actinin binding Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • muscle alpha-actinin binding Source: UniProtKB

GO - Biological processi

  • muscle structure development Source: FlyBase
  • myofibril assembly Source: FlyBase
  • regulation of cell-matrix adhesion Source: UniProtKB

Keywordsi

LigandMetal-binding, Zinc

Enzyme and pathway databases

SignaLinkiA1ZA47.

Names & Taxonomyi

Protein namesi
Recommended name:
PDZ and LIM domain protein Zasp
Alternative name(s):
Z band alternatively spliced PDZ-motif protein1 Publication
Gene namesi
Name:Zasp52
Synonyms:ZaspImported
ORF Names:CG30084
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0265991. Zasp52.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Fails to form the muscle Z line. At the myotendinous junction, muscles detach with the onset of contractility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004011061 – 2194PDZ and LIM domain protein ZaspAdd BLAST2194

Proteomic databases

PaxDbiA1ZA47.
PRIDEiA1ZA47.

Expressioni

Tissue specificityi

Expression is first detected in the proctodeum and the midgut primordium. In stage 11 embryos, expression is predominant in the leading edge of epidermal cells adjacent to the amnioserosa. Stage 12 embryos exhibit expression in the midgut and the leading edge. Expressed in several rows of germ band cells next to the leading edge at stage 14. Strong expression is visible in the midgut and pharyngeal muscles of stage 17 embryos. Also expressed in somatic muscles and visceral mesoderm. Colocalizes with mys (beta PS integrin) in myotendinous junctions and with Actn in muscle Z lines.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0265991.
ExpressionAtlasiA1ZA47. differential.
GenevisibleiA1ZA47. DM.

Interactioni

Subunit structurei

Interacts with alpha-actinin (Actn).1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actinin binding Source: FlyBase
  • muscle alpha-actinin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi62468. 36 interactors.
IntActiA1ZA47. 5 interactors.
STRINGi7227.FBpp0099801.

Structurei

3D structure databases

ProteinModelPortaliA1ZA47.
SMRiA1ZA47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 90PDZPROSITE-ProRule annotationAdd BLAST83
Domaini280 – 339LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST60
Domaini2018 – 2078LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST61
Domaini2079 – 2138LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST60
Domaini2139 – 2194LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi229 – 253Gln-richSequence analysisAdd BLAST25
Compositional biasi388 – 431Ala-richSequence analysisAdd BLAST44
Compositional biasi1148 – 1299Gln-richSequence analysisAdd BLAST152
Compositional biasi1606 – 1726Gln-richSequence analysisAdd BLAST121
Compositional biasi1841 – 1878Ser-richSequence analysisAdd BLAST38

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703. Eukaryota.
ENOG410XRD4. LUCA.
GeneTreeiENSGT00760000118910.
InParanoidiA1ZA47.
KOiK19867.
OMAiFEEEDCY.
OrthoDBiEOG091G05JI.
PhylomeDBiA1ZA47.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR031847. DUF4749.
IPR001478. PDZ.
IPR036034. PDZ_sf.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR006643. Zasp-like_motif.
IPR001781. Znf_LIM.
PfamiView protein in Pfam
PF15936. DUF4749. 1 hit.
PF00412. LIM. 4 hits.
PF00595. PDZ. 1 hit.
SMARTiView protein in SMART
SM00132. LIM. 4 hits.
SM00228. PDZ. 1 hit.
SM00735. ZM. 1 hit.
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiView protein in PROSITE
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
PS50106. PDZ. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform FImported (identifier: A1ZA47-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQPQLLQIK LSRFDAQPWG FRLQGGTDFA QPLLVQKVNA GSLSEQAGLQ
60 70 80 90 100
PGDAVVKIND VDVFNLRHKD AQDIVVRSGN NFVITVQRGG STWRPHVTPT
110 120 130 140 150
GNVPQPNSPY LQTVTKTSLA HKQQDSQHIG CGYNNAARPF SNGGDGGVKS
160 170 180 190 200
IVNKQYNTPV GIYSDESIAE TLSAQAEVLA GGVLGVNFKK NEKEYQGDRS
210 220 230 240 250
EVLKFLREEE TGQSTPAFGN SHYEHDAPQQ LQQPQQQYNQ HQQHYHQQQQ
260 270 280 290 300
QQQSSTTRHV SAPVNSPKPP STGGLPTGQN ICTECERLIT GVFVRIKDKN
310 320 330 340 350
LHVECFKCAT CGTSLKNQGY YNFNNKLYCD IHAKQAAINN PPTGTEGYVP
360 370 380 390 400
VPIKPNTKLS ASTISSALNS HGYGGHSNGY SNGNSTPAPA PVASSQATAT
410 420 430 440 450
VATVAPSAAT AATAAATPQA ATATDSPAAT ASSSDNMSAY VADEPSSIYG
460 470 480 490 500
QISAESVALA PPPPQPPTAG GGDQPFEYVT LTGNVIRSVQ APGKGACPSY
510 520 530 540 550
KVNQGYARPF GAAAPKSPVS YPPQQQQQSP RPAPGGQNPY ATLPRSNVGQ
560 570 580 590 600
QGGEAVEELQ PEFEEEDCYE MDIEVALAAS RQSQRGSSFT WPPPQDDSHL
610 620 630 640 650
APTAAPLYIP PPETQHVVVS NPVQQVPPLP PGGATARLDP QPVVGTSANG
660 670 680 690 700
APQWQSYSAP QLTTASARQL AEQESSSDSY TSTSTTTTTT SEEYQRMYAA
710 720 730 740 750
QVQAYQMQEQ SGSEFDYQVD YASTQDSVQD YPSGRRSAQE CVDSLAVPLS
760 770 780 790 800
TYKLVDMVRE VTPSPVTTPT QTPAPAAPTT RRVVFNDEPE IKELPQLPAE
810 820 830 840 850
LETIPEASEA VEDREGLVIE QRCQILESER KFQPTPEIKI EIAPVRQIPP
860 870 880 890 900
TKIPNPMPKE WINPMIRVLT TAPEVPFHLV ECPFPRPCGD DFEAEAAAAE
910 920 930 940 950
AAKTQEVPEP LPPQVSAAPP ATVSVEPSPA PLRESPPRGS RLSQAMVTAP
960 970 980 990 1000
EFELKFAPPA DQGIPLPEET EPYMPPPIDT KPYLREDYRP KSPFVSALTT
1010 1020 1030 1040 1050
APDRPFEGHF DKDVPIHMID LPTPKEHLSM CDALCTAPER GYTPLNPENA
1060 1070 1080 1090 1100
MHRVDEEQKQ QELKKREFQV LDHEEELGIR PEPPQSVEYY ETRRDQPRKS
1110 1120 1130 1140 1150
SAFAAMQAFQ PSREPLSSNT VSNAGSVADT PRASIVSALK EETDLEYQKY
1160 1170 1180 1190 1200
LKAQQRNQKR LDYFHQKEEE LSGLQGQQLT QLQRELSNQQ QNLLSQQQLQ
1210 1220 1230 1240 1250
QSKLLQLQQC VQSQELQQQV QHLTQKSQQQ PPQANQQQQQ QQQQRGTQQQ
1260 1270 1280 1290 1300
QHSQVTQRTQ QQQQQVPQQV TQQQQQEHSL LSQTTLAETQ TLQANAQSQS
1310 1320 1330 1340 1350
SASYSSKATA CSNSSSTVPP ANTSTAFAPA PAPAPTSIPV RPSAIAVQSS
1360 1370 1380 1390 1400
YCSSQFDVHE LIEETAEELE HSEVLFPPPS PLSHLTKQGK AVQSGLHKAD
1410 1420 1430 1440 1450
SIPKYQRNWT VLPTQSPIRT PEPQELRENV PLAFVDAPKA PVTSDSSTVH
1460 1470 1480 1490 1500
RPIAQVAAPT TVVAPSRERE KERRPQLSVP IIVEDRSGPV TMAFQPLDEL
1510 1520 1530 1540 1550
VRPDQALTPT RPYTPSLTNK PAPIVPFYQT EEKLVFEECS ATHARNYNEL
1560 1570 1580 1590 1600
NASPFPDRTR SPAPGPPPNP LNAIRAPRMK EPETKSNILS VSGGPRLQTG
1610 1620 1630 1640 1650
SITTGQSYQG QLLAHSEQSS QSASQSYNQQ PERITEQRVG NLNIQQREQS
1660 1670 1680 1690 1700
SQLQQQAQSQ TQSQTRSQVG NTQIERRRKV TEEFERTQSA KTIEIRTGSQ
1710 1720 1730 1740 1750
SVSQSKAQSQ SISQAQTQAQ SQSQNQSDTE RRSSYGKTGF VASQAKRLSC
1760 1770 1780 1790 1800
MEEEISSLTS QSQAISARAS ALGEGCFPNL RSPTFDSKFP LKPAPAESIV
1810 1820 1830 1840 1850
PGYATVPAAT KMLTAPPPGF LQQQQQQQQR SAFSGYQATT SSVQQSSFAS
1860 1870 1880 1890 1900
SSKATTSSLS SSSASASASA SVARSSQSLT QASAITTTTN NQATTAYRSS
1910 1920 1930 1940 1950
NGSITKPNLA SRPSIASITA PGSASAPAPV PSAAPTKATA PFKAPIVPKS
1960 1970 1980 1990 2000
VIANAVNAAA PPAPAVFPPD LSDLNLNSNV DNSPGAGGKS AGAFGATSAP
2010 2020 2030 2040 2050
KRGRGILNKA AGPGVRIPLC NSCNVQIRGP FITALGRIWC PDHFICVNGN
2060 2070 2080 2090 2100
CRRPLQDIGF VEEKGDLYCE YCFEKYLAPT CSKCAGKIKG DCLNAIGKHF
2110 2120 2130 2140 2150
HPECFTCGQC GKIFGNRPFF LEDGNAYCEA DWNELFTTKC FACGFPVEAG
2160 2170 2180 2190
DRWVEALNHN YHSQCFNCTF CKQNLEGQSF YNKGGRPFCK NHAR
Note: No experimental confirmation available.Curated
Length:2,194
Mass (Da):238,063
Last modified:November 13, 2007 - v2
Checksum:i97A349A08DDC7C8E
GO
Isoform CImported (identifier: A1ZA47-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-391: P → PPESESSQEL...ANQLKFNPHQ
     553-622: GEAVEELQPE...ETQHVVVSNP → RNVRYQQQQQ...SNYNNNNVYR
     623-1984: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:1,082
Mass (Da):116,653
Checksum:iFDF36C6D5665046A
GO
Isoform EImported (identifier: A1ZA47-4) [UniParc]FASTAAdd to basket
Also known as: Enigma1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     217-290: AFGNSHYEHD...ICTECERLIT → EPHSPANFYW...ACQLCGVGIV
     393-502: Missing.
     553-622: GEAVEELQPE...ETQHVVVSNP → RNVRYQQQQQ...SNYNNNNVYR
     623-1984: Missing.

Show »
Length:780
Mass (Da):85,417
Checksum:i334FC1B579778C31
GO
Isoform GImported (identifier: A1ZA47-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-290: AFGNSHYEHD...ICTECERLIT → EPHSPANFYW...ACQLCGVGIV
     553-622: GEAVEELQPE...ETQHVVVSNP → RNVRYQQQQQ...SNYNNNNVYR
     623-1984: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:890
Mass (Da):96,010
Checksum:i8876C68869DD85DF
GO
Isoform IImported (identifier: A1ZA47-7) [UniParc]FASTAAdd to basket
Also known as: Alp1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     393-502: Missing.
     552-560: GGEAVEELQ → VGYGYPYTY
     561-2194: Missing.

Show »
Length:450
Mass (Da):48,895
Checksum:iC4866509CD16325E
GO

Sequence cautioni

The sequence AAO39569 differs from that shown. Intron retention.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040043217 – 290AFGNS…ERLIT → EPHSPANFYWTQSHAIGGNE RRTPLHHQHQQPQQDERIGV PLQSNTLAPEATHRPSLPVA PKDNEEQARQDQQEQPDPRI IVLPICPGLQGPEYKAEMEA AAAALATDQDGRPRPLAASG HPACQLCGVGIV in isoform E and isoform G. 4 PublicationsAdd BLAST74
Alternative sequenceiVSP_040045391P → PPESESSQELPLPPPPSPTQ LLQYEAEQQVLPEPHMSTIQ QPQQQQKLEHTRTHSSLSSI SSGSSSSGVGGSGSGSGSGS GVGQSQQSYSSTLSLDRFGS PLHSRQTSGSSTSLEVALAA PGAGQGDNYTMTPPSPPPPP PPQAQSVTNYRLQAAQNEND MNTQNKSPNAYNQLLKEYSN KLQQQHHHNTTQHTTATTAP SLKHNNTAKPFAVAATSTPQ QHLPHQQHQQQPLVAALTAT LANQLKFNPHQ in isoform C. 1 Publication1
Alternative sequenceiVSP_040046393 – 502Missing in isoform I and isoform E. 3 PublicationsAdd BLAST110
Alternative sequenceiVSP_040048552 – 560GGEAVEELQ → VGYGYPYTY in isoform I. 2 Publications9
Alternative sequenceiVSP_040050553 – 622GEAVE…VVSNP → RNVRYQQQQQQQQQYNNQQK QQYRNSYPMGSNYSTPSQSP YITSNTNNYSSSNSYNNNNY SNYNNNNVYR in isoform C, isoform E and isoform G. 4 PublicationsAdd BLAST70
Alternative sequenceiVSP_040051561 – 2194Missing in isoform I. 2 PublicationsAdd BLAST1634
Alternative sequenceiVSP_040052623 – 1984Missing in isoform C, isoform E and isoform G. 4 PublicationsAdd BLAST1362

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF221635 mRNA. Translation: ABQ18241.1.
AE013599 Genomic DNA. Translation: AAF58107.5.
AE013599 Genomic DNA. Translation: AAM70963.2.
AE013599 Genomic DNA. Translation: AAZ52805.1.
AE013599 Genomic DNA. Translation: AAZ52806.2.
AE013599 Genomic DNA. Translation: ABI31098.1.
BT001752 mRNA. Translation: AAN71507.1.
BT003565 mRNA. Translation: AAO39569.1. Sequence problems.
BT011386 mRNA. Translation: AAR96178.1.
BT022171 mRNA. Translation: AAY51565.1.
RefSeqiNP_001027420.2. NM_001032249.4. [A1ZA47-1]
NP_001027421.1. NM_001032250.3. [A1ZA47-4]
NP_001036551.1. NM_001043086.3. [A1ZA47-5]
NP_611058.4. NM_137214.5.
NP_665700.2. NM_145757.3. [A1ZA47-3]
UniGeneiDm.22165.

Genome annotation databases

EnsemblMetazoaiFBtr0087315; FBpp0086449; FBgn0265991. [A1ZA47-3]
FBtr0100387; FBpp0099800; FBgn0265991. [A1ZA47-4]
FBtr0100388; FBpp0099801; FBgn0265991. [A1ZA47-1]
FBtr0111048; FBpp0110340; FBgn0265991. [A1ZA47-5]
GeneIDi36740.
KEGGidme:Dmel_CG30084.
UCSCiCG30084-RA. d. melanogaster.
CG30084-RC. d. melanogaster.
CG30084-RE. d. melanogaster.
CG30084-RF. d. melanogaster. [A1ZA47-1]
CG30084-RG. d. melanogaster.
CG30084-RH. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZASP_DROME
AccessioniPrimary (citable) accession number: A1ZA47
Secondary accession number(s): A1ZA48
, A1ZA49, A8DYF3, B0EW00, Q4V6Y5, Q6NNA6, Q86NZ1, Q8IGJ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 13, 2007
Last modified: November 22, 2017
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase