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A1Z9P3

- SHRM_DROME

UniProt

A1Z9P3 - SHRM_DROME

Protein

Protein Shroom

Gene

Shroom

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    May be involved in the assembly of microtubule arrays during cell elongation.By similarity

    GO - Molecular functioni

    1. actin binding Source: UniProtKB

    GO - Biological processi

    1. cell migration Source: UniProtKB
    2. cell morphogenesis Source: FlyBase
    3. melanosome organization Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Shroom
    Gene namesi
    Name:Shroom
    ORF Names:CG34379
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0085408. Shroom.

    Subcellular locationi

    Cytoplasmcytoskeleton 1 Publication
    Note: Associates with cortical F-actin.

    GO - Cellular componenti

    1. cortical actin cytoskeleton Source: FlyBase
    2. cytoskeleton Source: UniProtKB
    3. microtubule Source: UniProtKB-KW
    4. plasma membrane Source: UniProtKB
    5. tight junction Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15761576Protein ShroomPRO_0000286069Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei404 – 4041Phosphoserine1 Publication
    Modified residuei667 – 6671Phosphoserine1 Publication
    Modified residuei668 – 6681Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiA1Z9P3.

    Expressioni

    Gene expression databases

    BgeeiA1Z9P3.

    Interactioni

    Protein-protein interaction databases

    BioGridi62342. 10 interactions.

    Structurei

    Secondary structure

    1
    1576
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1398 – 144548
    Helixi1448 – 152780
    Helixi1530 – 156738

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3THFX-ray2.70A/B1393-1576[»]
    ProteinModelPortaliA1Z9P3.
    SMRiA1Z9P3. Positions 1396-1570.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1305 – 1572268ASD2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1232 – 129665Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi34 – 314281Ser-richAdd
    BLAST
    Compositional biasi221 – 23515Asn-richAdd
    BLAST
    Compositional biasi707 – 896190Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the shroom family.Curated
    Contains 1 ASD2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG72784.
    GeneTreeiENSGT00530000063061.
    InParanoidiQ3KN39.
    OMAiSSQRIVH.
    OrthoDBiEOG7B05C7.
    PhylomeDBiA1Z9P3.

    Family and domain databases

    InterProiIPR014799. ASD2.
    IPR027685. Shroom_fam.
    [Graphical view]
    PANTHERiPTHR15012. PTHR15012. 1 hit.
    PfamiPF08687. ASD2. 1 hit.
    [Graphical view]
    PROSITEiPS51307. ASD2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform D (identifier: A1Z9P3-3) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKMRNHKENG NGSEMGESTK SLAKMEPENN NKISVVSVSK LLLKDSNGAN     50
    SRSSNSNASF SSASVAGSVQ DDLPHHNSSS SQLGQQHGSS LDQCGLTQAG 100
    LEEYNNRSSS YYDQTAFHHQ KQPSYAQSEG YHSYVSSSDS TSATPFLDKL 150
    RQESDLLSRQ SHHWSENDLS SVCSNSVAPS PIPLLARQSH SHSHSHAHSH 200
    SNSHGHSHGH AHSASSSSSS NNNSNGSATN NNNNNSSEST SSTETLKWLG 250
    SMSDISEASH ATGYSAISES VSSSQRIVHS SRVPTPKRHH SESVLYLHNN 300
    EEQGDSSPTA SNSSQMMISE EANGEESPPS VQPLRIQHRH SPSYPPVHTS 350
    MVLHHFQQQQ QQQQDYQHPS RHHTNQSTLS TQSSLLELAS PTEKPRSLMG 400
    QSHSMGDLQQ KNPHQNPMLG RSAGQQHKSS ISVTISSSEA VVTIAPQPPA 450
    GKPSKLQLSL GKSEALSCST PNMGEQSPTN SIDSYRSNHR LFPVSTYTEP 500
    VHSNTSQYVQ HPKPQFSSGL HKSAKLPVIT PAGATVQPTW HSVAERINDF 550
    ERSQLGEPPK FAYLEPTKTH RLSNPALKAL QKNAVQSYVE RQQQQQKEEQ 600
    QLLRPHSQSY QACHVERKSL PNNLSPIMVG LPTGSNSAST RDCSSPTPPP 650
    PPRRSGSLLP NLLRRSSSAS DYAEFRELHQ AQGQVKGPSI RNISNAEKIS 700
    FNDCGMPPPP PPPRGRLAVP TRRTSSATEY APMRDKLLLQ QAAALAHQQH 750
    HPQQHRHAQP PHVPPERPPK HPNLRVPSPE LPPPPQSELD ISYTFDEPLP 800
    PPPPPEVLQP RPPPSPNRRN CFAGASTRRT TYEAPPPTAI VAAKVPPLVP 850
    KKPTSLQHKH LANGGGGSRK RPHHATPQPI LENVASPVAP PPPLLPRARS 900
    TAHDNVIASN LESNQQKRSN SKASYLPRQS LEKLNNTDPD HGIYKLTLTS 950
    NEDLVAHTKP SYGVTGKLPN NLPDVLPLGV KLHQQPKLQP GSPNGDANVT 1000
    LRYGSNNNLT GNSPTVAPPP YYGGGQRYST PVLGQGYGKS SKPVTPQQYT 1050
    RSQSYDVKHT SAVTMPTMSQ SHVDLKQAAH DLETTLEEVL PTATPTPTPT 1100
    PTPTPPRLSP ASSHSDCSLS TSSLECTINP IATPIPKPEA HIFRAEVIST 1150
    TLNTNPLTTP PKPAMNRQES LRENIEKITQ LQSVLMSAHL CDASLLGGYT 1200
    TPLITSPTAS FANEPLMTPP LPPSPPPPLE PEEEEEQEEN DVHDKQPEIE 1250
    ELQLMQRSEL VLMVNPKPST TDMACQTDEL EDRDTDLEAA REEHQTRTTL 1300
    QPRQRQPIEL DYEQMSRELV KLLPPGDKIA DILTPKICKP TSQYVSNLYN 1350
    PDVPLRLAKR DVGTSTLMRM KSITSSAEIR VVSVELQLAE PSEEPTNLIK 1400
    QKMDELIKHL NQKIVSLKRE QQTISEECSA NDRLGQDLFA KLAEKVRPSE 1450
    ASKFRTHVDA VGNITSLLLS LSERLAQTES SLETRQQERG ALESKRDLLY 1500
    EQMEEAQRLK SDIERRGVSI AGLLAKNLSA DMCADYDYFI NMKAKLIADA 1550
    RDLAVRIKGS EEQLSSLSDA LVQSDC 1576

    Note: No experimental confirmation available.

    Length:1,576
    Mass (Da):172,713
    Last modified:May 5, 2009 - v2
    Checksum:i52A7D124F9559D13
    GO
    Isoform F (identifier: A1Z9P3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-10: KMRNHKENG → AQWFQWLNF
         11-917: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:669
    Mass (Da):74,175
    Checksum:iF22B2C2E0F946C2B
    GO
    Isoform G (identifier: A1Z9P3-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-10: KMRNHKENG → VFGGKFRIR
         11-917: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:669
    Mass (Da):74,015
    Checksum:iCC509350FB342EDE
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 109KMRNHKENG → AQWFQWLNF in isoform F. 1 PublicationVSP_024971
    Alternative sequencei2 – 109KMRNHKENG → VFGGKFRIR in isoform G. CuratedVSP_036999
    Alternative sequencei11 – 917907Missing in isoform F and isoform G. 1 PublicationVSP_037000Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013599 Genomic DNA. Translation: AAF58259.2.
    AE013599 Genomic DNA. Translation: AAM70999.2.
    AE013599 Genomic DNA. Translation: AAF58260.2.
    AY069213 mRNA. Translation: AAL39358.1.
    BT023900 mRNA. Translation: ABA81834.1.
    RefSeqiNP_001097307.1. NM_001103837.1. [A1Z9P3-3]
    NP_610952.1. NM_137108.2. [A1Z9P3-2]
    NP_725378.2. NM_166047.1. [A1Z9P3-1]
    UniGeneiDm.2231.

    Genome annotation databases

    EnsemblMetazoaiFBtr0112614; FBpp0111526; FBgn0085408. [A1Z9P3-3]
    GeneIDi36592.
    KEGGidme:Dmel_CG34379.
    UCSCiCG34379-RD. d. melanogaster.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013599 Genomic DNA. Translation: AAF58259.2 .
    AE013599 Genomic DNA. Translation: AAM70999.2 .
    AE013599 Genomic DNA. Translation: AAF58260.2 .
    AY069213 mRNA. Translation: AAL39358.1 .
    BT023900 mRNA. Translation: ABA81834.1 .
    RefSeqi NP_001097307.1. NM_001103837.1. [A1Z9P3-3 ]
    NP_610952.1. NM_137108.2. [A1Z9P3-2 ]
    NP_725378.2. NM_166047.1. [A1Z9P3-1 ]
    UniGenei Dm.2231.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3THF X-ray 2.70 A/B 1393-1576 [» ]
    ProteinModelPortali A1Z9P3.
    SMRi A1Z9P3. Positions 1396-1570.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 62342. 10 interactions.

    Proteomic databases

    PRIDEi A1Z9P3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0112614 ; FBpp0111526 ; FBgn0085408 . [A1Z9P3-3 ]
    GeneIDi 36592.
    KEGGi dme:Dmel_CG34379.
    UCSCi CG34379-RD. d. melanogaster.

    Organism-specific databases

    CTDi 36592.
    FlyBasei FBgn0085408. Shroom.

    Phylogenomic databases

    eggNOGi NOG72784.
    GeneTreei ENSGT00530000063061.
    InParanoidi Q3KN39.
    OMAi SSQRIVH.
    OrthoDBi EOG7B05C7.
    PhylomeDBi A1Z9P3.

    Miscellaneous databases

    GenomeRNAii 36592.
    NextBioi 799392.

    Gene expression databases

    Bgeei A1Z9P3.

    Family and domain databases

    InterProi IPR014799. ASD2.
    IPR027685. Shroom_fam.
    [Graphical view ]
    PANTHERi PTHR15012. PTHR15012. 1 hit.
    Pfami PF08687. ASD2. 1 hit.
    [Graphical view ]
    PROSITEi PS51307. ASD2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM F).
      Strain: Berkeley.
      Tissue: Head.
    4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
      Strain: Berkeley.
      Tissue: Larva and Pupae.
    5. Cited for: NOMENCLATURE.
    6. "Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins."
      Dietz M.L., Bernaciak T.M., Vendetti F., Kielec J.M., Hildebrand J.D.
      J. Biol. Chem. 281:20542-20554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-404; SER-667 AND SER-668, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiSHRM_DROME
    AccessioniPrimary (citable) accession number: A1Z9P3
    Secondary accession number(s): A1Z9P2, Q3KN39, Q7K1B1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 49 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3