A1Z9P3 (SHRM_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Shroom

Gene names

Name:	Shroom
ORF Names:	CG34379

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1576 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the assembly of microtubule arrays during cell elongation By similarity.
Subcellular location	Cytoplasm › cytoskeleton. Note: Associates with cortical F-actin. Ref.6
Sequence similarities	Belongs to the shroom family. Contains 1 ASD2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell migration Inferred from sequence or structural similarity. Source: UniProtKB cell morphogenesis Inferred from direct assay Ref.6. Source: FlyBase melanosome organization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cortical actin cytoskeleton Inferred from direct assay Ref.6. Source: FlyBase microtubule Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB tight junction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	actin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform D (identifier: A1Z9P3-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform F (identifier: A1Z9P3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-10: KMRNHKENG → AQWFQWLNF
     11-917: Missing.

Note: No experimental confirmation available.

Isoform G (identifier: A1Z9P3-1)

The sequence of this isoform differs from the canonical sequence as follows:
     2-10: KMRNHKENG → VFGGKFRIR
     11-917: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1576

1576

Protein Shroom

PRO_0000286069

Regions

Domain

1305 – 1572

268

ASD2

Coiled coil

1232 – 1296

65

Potential

Compositional bias

34 – 314

281

Ser-rich

Compositional bias

221 – 235

15

Asn-rich

Compositional bias

707 – 896

190

Pro-rich

Amino acid modifications

Modified residue

404

1

Phosphoserine Ref.7

Modified residue

667

1

Phosphoserine Ref.7

Modified residue

668

1

Phosphoserine Ref.7

Natural variations

Alternative sequence

2 – 10

9

KMRNHKENG → AQWFQWLNF in isoform F.

VSP_024971

Alternative sequence

2 – 10

9

KMRNHKENG → VFGGKFRIR in isoform G.

VSP_036999

Alternative sequence

11 – 917

907

Missing in isoform F and isoform G.

VSP_037000

Secondary structure

1

.

.

.

.

.

.

.

1576

Helix Strand Turn

Details...

Helix

1398 – 1445

48

Helix

1448 – 1527

80

Helix

1530 – 1567

38

Sequences

	Sequence		Length	Mass (Da)
	Isoform D [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 52A7D124F9559D13	FASTA	1,576	172,713
	10 20 30 40 50 60 MKMRNHKENG NGSEMGESTK SLAKMEPENN NKISVVSVSK LLLKDSNGAN SRSSNSNASF 70 80 90 100 110 120 SSASVAGSVQ DDLPHHNSSS SQLGQQHGSS LDQCGLTQAG LEEYNNRSSS YYDQTAFHHQ 130 140 150 160 170 180 KQPSYAQSEG YHSYVSSSDS TSATPFLDKL RQESDLLSRQ SHHWSENDLS SVCSNSVAPS 190 200 210 220 230 240 PIPLLARQSH SHSHSHAHSH SNSHGHSHGH AHSASSSSSS NNNSNGSATN NNNNNSSEST 250 260 270 280 290 300 SSTETLKWLG SMSDISEASH ATGYSAISES VSSSQRIVHS SRVPTPKRHH SESVLYLHNN 310 320 330 340 350 360 EEQGDSSPTA SNSSQMMISE EANGEESPPS VQPLRIQHRH SPSYPPVHTS MVLHHFQQQQ 370 380 390 400 410 420 QQQQDYQHPS RHHTNQSTLS TQSSLLELAS PTEKPRSLMG QSHSMGDLQQ KNPHQNPMLG 430 440 450 460 470 480 RSAGQQHKSS ISVTISSSEA VVTIAPQPPA GKPSKLQLSL GKSEALSCST PNMGEQSPTN 490 500 510 520 530 540 SIDSYRSNHR LFPVSTYTEP VHSNTSQYVQ HPKPQFSSGL HKSAKLPVIT PAGATVQPTW 550 560 570 580 590 600 HSVAERINDF ERSQLGEPPK FAYLEPTKTH RLSNPALKAL QKNAVQSYVE RQQQQQKEEQ 610 620 630 640 650 660 QLLRPHSQSY QACHVERKSL PNNLSPIMVG LPTGSNSAST RDCSSPTPPP PPRRSGSLLP 670 680 690 700 710 720 NLLRRSSSAS DYAEFRELHQ AQGQVKGPSI RNISNAEKIS FNDCGMPPPP PPPRGRLAVP 730 740 750 760 770 780 TRRTSSATEY APMRDKLLLQ QAAALAHQQH HPQQHRHAQP PHVPPERPPK HPNLRVPSPE 790 800 810 820 830 840 LPPPPQSELD ISYTFDEPLP PPPPPEVLQP RPPPSPNRRN CFAGASTRRT TYEAPPPTAI 850 860 870 880 890 900 VAAKVPPLVP KKPTSLQHKH LANGGGGSRK RPHHATPQPI LENVASPVAP PPPLLPRARS 910 920 930 940 950 960 TAHDNVIASN LESNQQKRSN SKASYLPRQS LEKLNNTDPD HGIYKLTLTS NEDLVAHTKP 970 980 990 1000 1010 1020 SYGVTGKLPN NLPDVLPLGV KLHQQPKLQP GSPNGDANVT LRYGSNNNLT GNSPTVAPPP 1030 1040 1050 1060 1070 1080 YYGGGQRYST PVLGQGYGKS SKPVTPQQYT RSQSYDVKHT SAVTMPTMSQ SHVDLKQAAH 1090 1100 1110 1120 1130 1140 DLETTLEEVL PTATPTPTPT PTPTPPRLSP ASSHSDCSLS TSSLECTINP IATPIPKPEA 1150 1160 1170 1180 1190 1200 HIFRAEVIST TLNTNPLTTP PKPAMNRQES LRENIEKITQ LQSVLMSAHL CDASLLGGYT 1210 1220 1230 1240 1250 1260 TPLITSPTAS FANEPLMTPP LPPSPPPPLE PEEEEEQEEN DVHDKQPEIE ELQLMQRSEL 1270 1280 1290 1300 1310 1320 VLMVNPKPST TDMACQTDEL EDRDTDLEAA REEHQTRTTL QPRQRQPIEL DYEQMSRELV 1330 1340 1350 1360 1370 1380 KLLPPGDKIA DILTPKICKP TSQYVSNLYN PDVPLRLAKR DVGTSTLMRM KSITSSAEIR 1390 1400 1410 1420 1430 1440 VVSVELQLAE PSEEPTNLIK QKMDELIKHL NQKIVSLKRE QQTISEECSA NDRLGQDLFA 1450 1460 1470 1480 1490 1500 KLAEKVRPSE ASKFRTHVDA VGNITSLLLS LSERLAQTES SLETRQQERG ALESKRDLLY 1510 1520 1530 1540 1550 1560 EQMEEAQRLK SDIERRGVSI AGLLAKNLSA DMCADYDYFI NMKAKLIADA RDLAVRIKGS 1570 EEQLSSLSDA LVQSDC
	Isoform F [UniParc]. Checksum: F22B2C2E0F946C2B	FASTA	669	74,175
	10 20 30 40 50 60 MAQWFQWLNF RSNSKASYLP RQSLEKLNNT DPDHGIYKLT LTSNEDLVAH TKPSYGVTGK 70 80 90 100 110 120 LPNNLPDVLP LGVKLHQQPK LQPGSPNGDA NVTLRYGSNN NLTGNSPTVA PPPYYGGGQR 130 140 150 160 170 180 YSTPVLGQGY GKSSKPVTPQ QYTRSQSYDV KHTSAVTMPT MSQSHVDLKQ AAHDLETTLE 190 200 210 220 230 240 EVLPTATPTP TPTPTPTPPR LSPASSHSDC SLSTSSLECT INPIATPIPK PEAHIFRAEV 250 260 270 280 290 300 ISTTLNTNPL TTPPKPAMNR QESLRENIEK ITQLQSVLMS AHLCDASLLG GYTTPLITSP 310 320 330 340 350 360 TASFANEPLM TPPLPPSPPP PLEPEEEEEQ EENDVHDKQP EIEELQLMQR SELVLMVNPK 370 380 390 400 410 420 PSTTDMACQT DELEDRDTDL EAAREEHQTR TTLQPRQRQP IELDYEQMSR ELVKLLPPGD 430 440 450 460 470 480 KIADILTPKI CKPTSQYVSN LYNPDVPLRL AKRDVGTSTL MRMKSITSSA EIRVVSVELQ 490 500 510 520 530 540 LAEPSEEPTN LIKQKMDELI KHLNQKIVSL KREQQTISEE CSANDRLGQD LFAKLAEKVR 550 560 570 580 590 600 PSEASKFRTH VDAVGNITSL LLSLSERLAQ TESSLETRQQ ERGALESKRD LLYEQMEEAQ 610 620 630 640 650 660 RLKSDIERRG VSIAGLLAKN LSADMCADYD YFINMKAKLI ADARDLAVRI KGSEEQLSSL SDALVQSDC
	Isoform G [UniParc]. Checksum: CC509350FB342EDE	FASTA	669	74,015
	10 20 30 40 50 60 MVFGGKFRIR RSNSKASYLP RQSLEKLNNT DPDHGIYKLT LTSNEDLVAH TKPSYGVTGK 70 80 90 100 110 120 LPNNLPDVLP LGVKLHQQPK LQPGSPNGDA NVTLRYGSNN NLTGNSPTVA PPPYYGGGQR 130 140 150 160 170 180 YSTPVLGQGY GKSSKPVTPQ QYTRSQSYDV KHTSAVTMPT MSQSHVDLKQ AAHDLETTLE 190 200 210 220 230 240 EVLPTATPTP TPTPTPTPPR LSPASSHSDC SLSTSSLECT INPIATPIPK PEAHIFRAEV 250 260 270 280 290 300 ISTTLNTNPL TTPPKPAMNR QESLRENIEK ITQLQSVLMS AHLCDASLLG GYTTPLITSP 310 320 330 340 350 360 TASFANEPLM TPPLPPSPPP PLEPEEEEEQ EENDVHDKQP EIEELQLMQR SELVLMVNPK 370 380 390 400 410 420 PSTTDMACQT DELEDRDTDL EAAREEHQTR TTLQPRQRQP IELDYEQMSR ELVKLLPPGD 430 440 450 460 470 480 KIADILTPKI CKPTSQYVSN LYNPDVPLRL AKRDVGTSTL MRMKSITSSA EIRVVSVELQ 490 500 510 520 530 540 LAEPSEEPTN LIKQKMDELI KHLNQKIVSL KREQQTISEE CSANDRLGQD LFAKLAEKVR 550 560 570 580 590 600 PSEASKFRTH VDAVGNITSL LLSLSERLAQ TESSLETRQQ ERGALESKRD LLYEQMEEAQ 610 620 630 640 650 660 RLKSDIERRG VSIAGLLAKN LSADMCADYD YFINMKAKLI ADARDLAVRI KGSEEQLSSL SDALVQSDC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM F). Strain: Berkeley. Tissue: Head.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D). Strain: Berkeley. Tissue: Larva and Pupae.
[5]	"A new standard nomenclature for proteins related to Apx and Shroom." Hagens O., Ballabio A., Kalscheuer V., Kraehenbuhl J.-P., Schiaffino M.V., Smith P., Staub O., Hildebrand J.D., Wallingford J.B. BMC Cell Biol. 7:18-18(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[6]	"Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins." Dietz M.L., Bernaciak T.M., Vendetti F., Kielec J.M., Hildebrand J.D. J. Biol. Chem. 281:20542-20554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-404; SER-667 AND SER-668, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE013599 Genomic DNA. Translation: AAF58259.2.
AE013599 Genomic DNA. Translation: AAM70999.2.
AE013599 Genomic DNA. Translation: AAF58260.2.
AY069213 mRNA. Translation: AAL39358.1.
BT023900 mRNA. Translation: ABA81834.1.

RefSeq

NP_001097307.1. NM_001103837.1.
NP_610952.1. NM_137108.2.
NP_725378.2. NM_166047.1.

UniGene

Dm.2231.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3THF	X-ray	2.70	A/B	1393-1576	[»]

ProteinModelPortal

A1Z9P3.

SMR

A1Z9P3. Positions 1396-1570.

ModBase

Search...

Proteomic databases

PRIDE

A1Z9P3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0112614; FBpp0111526; FBgn0085408.

GeneID

36592.

KEGG

dme:Dmel_CG34379.

Organism-specific databases

CTD

36592.

FlyBase

FBgn0085408. Shroom.

Phylogenomic databases

eggNOG

NOG72784.

GeneTree

ENSGT00530000063061.

InParanoid

Q3KN39.

OMA

AYLEPTK.

OrthoDB

EOG4WSTSZ.

PhylomeDB

A1Z9P3.

Gene expression databases

Bgee

A1Z9P3.

Family and domain databases

InterPro

IPR014799. ASD2.
[Graphical view]

Pfam

PF08687. ASD2. 1 hit.
[Graphical view]

PROSITE

PS51307. ASD2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

36592.

NextBio

799392.

Entry information

Entry name

SHRM_DROME

Accession

Primary (citable) accession number: A1Z9P3
Secondary accession number(s): A1Z9P2, Q3KN39, Q7K1B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 5, 2009
Last modified:	April 3, 2013
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project