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Protein

PAN2-PAN3 deadenylation complex catalytic subunit PAN2

Gene

PAN2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.UniRule annotation1 Publication

Cofactori

a divalent metal cationUniRule annotationNote: Binds 2 metal cations per subunit in the catalytic exonuclease domain.UniRule annotation

Enzyme regulationi

Positively regulated by the regulatory subunit PAN3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1039Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1041Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1148Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1200Divalent metal cation; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • nuclear-transcribed mRNA poly(A) tail shortening Source: FlyBase

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processmRNA processing
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-DME-429947. Deadenylation of mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
PAN2-PAN3 deadenylation complex catalytic subunit PAN2UniRule annotation (EC:3.1.13.4UniRule annotation)
Alternative name(s):
PAB1P-dependent poly(A)-specific ribonucleaseUniRule annotation
Poly(A)-nuclease deadenylation complex subunit 2UniRule annotation
Short name:
PAN deadenylation complex subunit 2UniRule annotation
Gene namesi
Name:PAN2UniRule annotation
ORF Names:CG8232
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0033352. PAN2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004416731 – 1241PAN2-PAN3 deadenylation complex catalytic subunit PAN2Add BLAST1241

Expressioni

Gene expression databases

BgeeiFBgn0033352.
ExpressionAtlasiA1Z7K9. differential.

Interactioni

Subunit structurei

Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.UniRule annotation1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiA1Z7K9. 2 interactors.
STRINGi7227.FBpp0087735.

Structurei

3D structure databases

SMRiA1Z7K9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini474 – 966USPUniRule annotationAdd BLAST493
Domaini1036 – 1208ExonucleaseUniRule annotationAdd BLAST173

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 473LinkerUniRule annotationAdd BLAST115

Domaini

Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.UniRule annotation
The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. PAN2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG1275. Eukaryota.
COG0847. LUCA.
OMAiMVYDLRM.
OrthoDBiEOG091G01KS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_03182. PAN2. 1 hit.
InterProiView protein in InterPro
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR030843. PAN2.
IPR028881. PAN2_dom.
IPR012337. RNaseH-like_sf.
IPR036397. RNaseH_sf.
IPR028889. USP_dom.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF00929. RNase_T. 1 hit.
PF13423. UCH_1. 1 hit.
SMARTiView protein in SMART
SM00479. EXOIII. 1 hit.
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiView protein in PROSITE
PS50235. USP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

A1Z7K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYVYCGTDP IGASEDILSV YDAGSAPGNG HFSPSFNGFN IGTTDPEYVE
60 70 80 90 100
LVPVLADGGE HFGVSSVAFD DYEELLWMGN QGGHVTSYYT NSMQKYTSFQ
110 120 130 140 150
VHATDIVRDI STLDSGVLAL TQTSLRHQIR RGLPKFTFKS NNMKEMVSML
160 170 180 190 200
QLSPHRLVMA GLQDELIDFD LRTLKETRIE HVGAGGCTVL RKNSRYLFAG
210 220 230 240 250
DQLGTVTLRD LNSLSVQHTI KTHTNILSDF SVQGNLLISC GYSGRQNNLA
260 270 280 290 300
IDRFLMVYDL RMLRLIAPIQ VMIDPQMLKF LPSLTSQLAV VSSYGQVQLV
310 320 330 340 350
DTVELSEPRV SMYQINTNGS QCLSFDISSS SQAMAFGDQS GHINMIAAVQ
360 370 380 390 400
TPQPQFNLYS RSTEFADVVP QLPMVSITDT NFPLSSVMLP HLTTGTQWFS
410 420 430 440 450
DWPEELLRYR YHRPKTIDPE VLSNMKMQGP IGYSPNPRTA RRNQIPYVIE
460 470 480 490 500
QGGVCSPNGN GTAAATKAEN GVKIIPRRYR KVELKYTKLG TQDFDFDQHN
510 520 530 540 550
QTCFAGLEAT LPNSYCNAML QILYFTDALR VKLLEHSCIK EFCLSCELGF
560 570 580 590 600
LFNMLDKSTA SSPCQASNFL RSFRTVPEAS ALGLILTDRS SNVNLISLIQ
610 620 630 640 650
NWNRFILHQM HYEIFDSSKN ASTYSGSVQT STNAENAGSS ETSGSSDLYD
660 670 680 690 700
SISDENSKED DRERSKINAE TDISKIFGTK QICINRCIKC QEEKIKESIL
710 720 730 740 750
LACNLSYPNH IKDSDQYFNF GTILKRSLSS EKSIQAFCER CKKFSPTNQS
760 770 780 790 800
VKVTSLPQIL SINCGLNNEK DITFLKRQLN RCSEKTTVDA AASLSTSKPC
810 820 830 840 850
RYGANCSRSD CHFMHPDRKS PSHTSQPNAV NNSPNGRQKS WFPLTFTMGI
860 870 880 890 900
NDQGEVQVQT QSDASSGKSE QEEETEKPPT KGLDNNRMYA LHAVVCQVDD
910 920 930 940 950
GTQKNLVSLI NVQRPYHTMK LAESADDPQS QWYIFNDFSI SPVSPQESVW
960 970 980 990 1000
FTLDWKVPCI LFYRHVEDDS ESASTTSSTV TESEETIPSE SSSGSPTNLS
1010 1020 1030 1040 1050
NPFLEEIVSP MLGNLSADAT LQPLQSDEMP QSGDLVAMDA EFVTLNPEEN
1060 1070 1080 1090 1100
EIRPDGKTAT IKPCHMSVAR ISCIRGQGPA EGVPFMDDYI STQEKVVDYL
1110 1120 1130 1140 1150
TQFSGIKPGD LDANFSKKRL TALKYSYQKL KYLVDVGVIF VGHGLKNDFR
1160 1170 1180 1190 1200
VINIYVPSEQ IIDTVHLFHM PHHRMVSLRF LAWHFLGTKI QSETHDSIED
1210 1220 1230 1240
ARTTLQLYKH YLKLQEEKKF ANALKNLYER GKQLQWKVPE D
Length:1,241
Mass (Da):138,968
Last modified:February 6, 2007 - v1
Checksum:iBFFE4AB585B1FB5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF59018.2.
BT031142 mRNA. Translation: ABX00764.1.
RefSeqiNP_610427.2. NM_136583.4.
UniGeneiDm.11534.

Genome annotation databases

EnsemblMetazoaiFBtr0088654; FBpp0087735; FBgn0033352.
GeneIDi35893.
UCSCiCG8232-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiPAN2_DROME
AccessioniPrimary (citable) accession number: A1Z7K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2017
Last sequence update: February 6, 2007
Last modified: November 22, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families