Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PAB-dependent poly(A)-specific ribonuclease subunit PAN2

Gene

PAN2

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.UniRule annotation

Enzyme regulationi

Positively regulated by the regulatory subunit PAN3.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

ExonucleaseUniRule annotation, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processingUniRule annotation

Enzyme and pathway databases

ReactomeiR-DME-429947. Deadenylation of mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
PAB-dependent poly(A)-specific ribonuclease subunit PAN2UniRule annotation (EC:3.1.13.4UniRule annotation)
Alternative name(s):
PAB1P-dependent poly(A)-nucleaseUniRule annotation
PAN deadenylation complex catalytic subunit 2UniRule annotation
Gene namesi
Name:PAN2UniRule annotationImported
ORF Names:CG8232Imported, Dmel_CG8232Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0033352. PAN2.

Subcellular locationi

  • CytoplasmP-body UniRule annotation
  • Nucleus UniRule annotation

  • Note: Shuttles between nucleus and cytoplasm.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Expressioni

Gene expression databases

BgeeiFBgn0033352.

Interactioni

Subunit structurei

Interacts with the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.UniRule annotation

Protein-protein interaction databases

STRINGi7227.FBpp0087735.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini505 – 966USPInterPro annotationAdd BLAST462

Sequence similaritiesi

Belongs to the peptidase C19 family. PAN2 subfamily.UniRule annotation
Contains 1 USP domain.UniRule annotation
Contains 1 exonuclease domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG1275. Eukaryota.
COG0847. LUCA.
GeneTreeiENSGT00390000013978.
OMAiILHQMHY.
OrthoDBiEOG091G01KS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_03182. PAN2. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR030843. PAN2.
IPR028881. PAN2_dom.
IPR012337. RNaseH-like_dom.
IPR028889. USP_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
PF13423. UCH_1. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1Z7K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYVYCGTDP IGASEDILSV YDAGSAPGNG HFSPSFNGFN IGTTDPEYVE
60 70 80 90 100
LVPVLADGGE HFGVSSVAFD DYEELLWMGN QGGHVTSYYT NSMQKYTSFQ
110 120 130 140 150
VHATDIVRDI STLDSGVLAL TQTSLRHQIR RGLPKFTFKS NNMKEMVSML
160 170 180 190 200
QLSPHRLVMA GLQDELIDFD LRTLKETRIE HVGAGGCTVL RKNSRYLFAG
210 220 230 240 250
DQLGTVTLRD LNSLSVQHTI KTHTNILSDF SVQGNLLISC GYSGRQNNLA
260 270 280 290 300
IDRFLMVYDL RMLRLIAPIQ VMIDPQMLKF LPSLTSQLAV VSSYGQVQLV
310 320 330 340 350
DTVELSEPRV SMYQINTNGS QCLSFDISSS SQAMAFGDQS GHINMIAAVQ
360 370 380 390 400
TPQPQFNLYS RSTEFADVVP QLPMVSITDT NFPLSSVMLP HLTTGTQWFS
410 420 430 440 450
DWPEELLRYR YHRPKTIDPE VLSNMKMQGP IGYSPNPRTA RRNQIPYVIE
460 470 480 490 500
QGGVCSPNGN GTAAATKAEN GVKIIPRRYR KVELKYTKLG TQDFDFDQHN
510 520 530 540 550
QTCFAGLEAT LPNSYCNAML QILYFTDALR VKLLEHSCIK EFCLSCELGF
560 570 580 590 600
LFNMLDKSTA SSPCQASNFL RSFRTVPEAS ALGLILTDRS SNVNLISLIQ
610 620 630 640 650
NWNRFILHQM HYEIFDSSKN ASTYSGSVQT STNAENAGSS ETSGSSDLYD
660 670 680 690 700
SISDENSKED DRERSKINAE TDISKIFGTK QICINRCIKC QEEKIKESIL
710 720 730 740 750
LACNLSYPNH IKDSDQYFNF GTILKRSLSS EKSIQAFCER CKKFSPTNQS
760 770 780 790 800
VKVTSLPQIL SINCGLNNEK DITFLKRQLN RCSEKTTVDA AASLSTSKPC
810 820 830 840 850
RYGANCSRSD CHFMHPDRKS PSHTSQPNAV NNSPNGRQKS WFPLTFTMGI
860 870 880 890 900
NDQGEVQVQT QSDASSGKSE QEEETEKPPT KGLDNNRMYA LHAVVCQVDD
910 920 930 940 950
GTQKNLVSLI NVQRPYHTMK LAESADDPQS QWYIFNDFSI SPVSPQESVW
960 970 980 990 1000
FTLDWKVPCI LFYRHVEDDS ESASTTSSTV TESEETIPSE SSSGSPTNLS
1010 1020 1030 1040 1050
NPFLEEIVSP MLGNLSADAT LQPLQSDEMP QSGDLVAMDA EFVTLNPEEN
1060 1070 1080 1090 1100
EIRPDGKTAT IKPCHMSVAR ISCIRGQGPA EGVPFMDDYI STQEKVVDYL
1110 1120 1130 1140 1150
TQFSGIKPGD LDANFSKKRL TALKYSYQKL KYLVDVGVIF VGHGLKNDFR
1160 1170 1180 1190 1200
VINIYVPSEQ IIDTVHLFHM PHHRMVSLRF LAWHFLGTKI QSETHDSIED
1210 1220 1230 1240
ARTTLQLYKH YLKLQEEKKF ANALKNLYER GKQLQWKVPE D
Length:1,241
Mass (Da):138,968
Last modified:February 6, 2007 - v1
Checksum:iBFFE4AB585B1FB5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF59018.2.
BT031142 mRNA. Translation: ABX00764.1.
RefSeqiNP_610427.2. NM_136583.4.
UniGeneiDm.11534.

Genome annotation databases

EnsemblMetazoaiFBtr0088654; FBpp0087735; FBgn0033352.
GeneIDi35893.
UCSCiCG8232-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF59018.2.
BT031142 mRNA. Translation: ABX00764.1.
RefSeqiNP_610427.2. NM_136583.4.
UniGeneiDm.11534.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0087735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088654; FBpp0087735; FBgn0033352.
GeneIDi35893.
UCSCiCG8232-RA. d. melanogaster.

Organism-specific databases

CTDi9924.
FlyBaseiFBgn0033352. PAN2.

Phylogenomic databases

eggNOGiKOG1275. Eukaryota.
COG0847. LUCA.
GeneTreeiENSGT00390000013978.
OMAiILHQMHY.
OrthoDBiEOG091G01KS.

Enzyme and pathway databases

ReactomeiR-DME-429947. Deadenylation of mRNA.

Miscellaneous databases

GenomeRNAii35893.

Gene expression databases

BgeeiFBgn0033352.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_03182. PAN2. 1 hit.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR030843. PAN2.
IPR028881. PAN2_dom.
IPR012337. RNaseH-like_dom.
IPR028889. USP_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
PF13423. UCH_1. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA1Z7K9_DROME
AccessioniPrimary (citable) accession number: A1Z7K9
Entry historyi
Integrated into UniProtKB/TrEMBL: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.