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Protein

Protein prickle

Gene

pk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts in a planar cell polarity (PCP) complex; polarization along the apical/basal axis of epithelial cells. Correct expression of the alternative isoforms is required for PCP signaling in imaginal disks. PCP signaling in the wing disk requires the receptor fz and the cytoplasmic proteins dsh and pk. These act in a feedback loop leading to activation of the jnk cascade and subsequent polarized arrangement of hairs and bristles. Dgo and pk compete with one another for dsh binding, thereby modulating fz dsh activity and ensuring tight control over fz PCP signaling. Vang, stan and pk function together to regulate the establishment of tissue polarity in the adult eye.3 Publications

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior axis specification Source: UniProtKB
  • axonal transport Source: FlyBase
  • establishment of imaginal disc-derived wing hair orientation Source: FlyBase
  • establishment of ommatidial planar polarity Source: FlyBase
  • establishment of planar polarity Source: UniProtKB
  • establishment of protein localization Source: FlyBase
  • establishment of tissue polarity Source: UniProtKB
  • establishment or maintenance of cell polarity Source: FlyBase
  • establishment or maintenance of microtubule cytoskeleton polarity Source: FlyBase
  • imaginal disc-derived leg joint morphogenesis Source: FlyBase
  • maintenance of protein location Source: FlyBase
  • morphogenesis of a polarized epithelium Source: FlyBase
  • negative regulation of Notch signaling pathway Source: FlyBase
  • positive regulation of axon extension Source: FlyBase
  • positive regulation of axon guidance Source: FlyBase
  • protein localization Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-4608870. Asymmetric localization of PCP proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein prickle
Alternative name(s):
Protein spiny legs
Gene namesi
Name:pkImported
ORF Names:CG11084
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003090. pk.

Subcellular locationi

GO - Cellular componenti

  • axon Source: GOC
  • cytoplasm Source: FlyBase
  • membrane Source: FlyBase
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Flies exhibit aberrant hair and bristle orientation on the wings and aberrant ommatidial arrangement in the compound eye.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002888341 – 1299Protein prickleAdd BLAST1299

Proteomic databases

PaxDbiA1Z6W3.
PRIDEiA1Z6W3.

Expressioni

Tissue specificityi

Expressed in the wing, leg and eye imaginal disks. Expressed within the photoreceptors of the eye.4 Publications

Developmental stagei

Expressed both maternally and zygotically. Isoform B is expressed in embryos only. Isoform A and isoform C are expressed in embryos and pupae.1 Publication

Gene expression databases

BgeeiFBgn0003090.
ExpressionAtlasiA1Z6W3. baseline.
GenevisibleiA1Z6W3. DM.

Interactioni

Subunit structurei

Interacts with dsh; PET and LIM domains interact with dsh DEP domain, in wing cells. Interacts with Vang in photoreceptor cells.4 Publications

Protein-protein interaction databases

BioGridi69608. 8 interactors.
DIPiDIP-59584N.
IntActiA1Z6W3. 2 interactors.
STRINGi7227.FBpp0088115.

Structurei

3D structure databases

ProteinModelPortaliA1Z6W3.
SMRiA1Z6W3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini515 – 623PETPROSITE-ProRule annotationAdd BLAST109
Domaini622 – 686LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST65
Domaini687 – 747LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST61
Domaini748 – 810LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi245 – 292Pro-richSequence analysisAdd BLAST48
Compositional biasi412 – 490Pro-richSequence analysisAdd BLAST79
Compositional biasi857 – 877Gln-richSequence analysisAdd BLAST21
Compositional biasi1157 – 1249Ser-richSequence analysisAdd BLAST93

Sequence similaritiesi

Belongs to the prickle / espinas / testin family.Curated
Contains 3 LIM zinc-binding domains.PROSITE-ProRule annotation
Contains 1 PET domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1704. Eukaryota.
ENOG410XP0W. LUCA.
GeneTreeiENSGT00550000074438.
InParanoidiA1Z6W3.
KOiK04511.
OMAiPKPMELQ.
OrthoDBiEOG091G07C5.
PhylomeDBiA1Z6W3.

Family and domain databases

CDDicd09415. LIM1_Prickle. 1 hit.
cd09418. LIM2_Prickle. 1 hit.
cd09420. LIM3_Prickle. 1 hit.
cd09827. PET_Prickle. 1 hit.
Gene3Di2.10.110.10. 3 hits.
InterProiIPR033725. LIM1_prickle.
IPR033726. LIM2_prickle.
IPR033727. LIM3_prickle.
IPR010442. PET_domain.
IPR033723. PET_prickle.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C1 Publication (identifier: A1Z6W3-1) [UniParc]FASTAAdd to basket
Also known as: sple1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSLSTGGGA GGSSGGPGGA DAAAAPAAGQ ATVTATGNME PAMVPRTANL
60 70 80 90 100
LACKQWWRVC FLYGDQQKYY RQLYSKAAAQ RLADANQEPD NARDREYDTV
110 120 130 140 150
DCDLIAGQLD AVEDADDGID LGDHSSTPKG GATTAGRPLF PHSSSPRRSK
160 170 180 190 200
KLLRSLRAHV RGEKLPKNDT TTANESSEVT QRNARVTVLD DPFLFGIDAD
210 220 230 240 250
HLGDLVVRGK RYSTLDATEN MARFYAEQEA TAQVLEIIEQ EEESPEQEAP
260 270 280 290 300
KPALPPKQKQ QRPVPPLPPP PANRVTQDQG TQPAAPQVPL QPLTAGDLQF
310 320 330 340 350
LNLSLRQRSL PRSMKPFKDA HDISFTFNEL DTSAEPEVAT GAAQQESNEP
360 370 380 390 400
ISRTPLTQIS YLQKIPTLPR HFSPSGQGLA TPPALGSGGM GLPSSSSASA
410 420 430 440 450
LYAAQAAAGI LPTSPLPLQR HQQYLPPHHQ QHPGAGMGPG PGSGAAAGPP
460 470 480 490 500
LGPQYSPGCS ANPKYSNAQL PPPPHHHHQL SPALSTPSPP SLLHHPAGGT
510 520 530 540 550
SSASAHAPFL GGPHMDMQRQ SHSDDDSGCA LEEYTWVPPG LRPDQVRLYF
560 570 580 590 600
SQIPDDKVPY VNSPGEQYRV RQLLHQLPPH DNEVRYCHSL TDEERKELRL
610 620 630 640 650
FSTQRKRDAL GRGNVRQLMS ARPCDGCDDL ISTGDIAVFA TRLGPNASWH
660 670 680 690 700
PACFACSVCR ELLVDLIYFH RDGRMYCGRH HAETLKPRCS ACDEIILADE
710 720 730 740 750
CTEAEGRAWH MNHFACHECD KQLGGQRYIM REGKPYCLHC FDAMFAEYCD
760 770 780 790 800
YCGEAIGVDQ GQMSHDGQHW HATDECFSCN TCRCSLLGRA FLPRRGAIYC
810 820 830 840 850
SIACSKGEPP TPSDSSGTGM YTTPTPPTQR VRPHPQAPLP ARIPSSHASS
860 870 880 890 900
SPPMSPQQQQ QHQATFNQAM YQMQSQQMEA AGGLVDQSKS YAASDSDAGV
910 920 930 940 950
VKDLEHGGHM GGGDLTDFSG GRASSTSQNL SPLNSPGDFQ PHFLPKPMEL
960 970 980 990 1000
QRDGVYNFNE MSSNLDAAWS AKPTNSYHLQ RQLLENPHTA SMPELAGKLV
1010 1020 1030 1040 1050
APPAHMQHLS QLHAVSSHQF QQHEYADILH PPPPPPGEIP ELPTPNLSVA
1060 1070 1080 1090 1100
STALPPELMG SPTHSAGDRS LNTPMSTQSA SHAPPHPVSI LSGASSSSPM
1110 1120 1130 1140 1150
SGEPAKKKGV RFEGIPDTLP RSRSYSGNGA GTSGGGERER DRDKDKEGGG
1160 1170 1180 1190 1200
RHGHGHSSRR RRRRKSSSSS SHHRSGSGHR SHSTTRADTY APAQPLSSSY
1210 1220 1230 1240 1250
QGPPSVLQAA NLVHESPSRQ QRERERERER EESEESDVCS TCSSSSSSSE
1260 1270 1280 1290
DYMMMYQLPQ RRHYGGVRVS YVPNDALAYD RKRKPSELGG DKDKNCIIS
Length:1,299
Mass (Da):140,722
Last modified:February 6, 2007 - v1
Checksum:i8BFAF1F75F352485
GO
Isoform A1 Publication (identifier: A1Z6W3-2) [UniParc]FASTAAdd to basket
Also known as: pk1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     2-13: SSLSTGGGAGGS → DTPNQMPVELER
     14-349: Missing.

Show »
Length:963
Mass (Da):104,682
Checksum:iC01685B61EA4C367
GO
Isoform B1 Publication (identifier: A1Z6W3-3) [UniParc]FASTAAdd to basket
Also known as: pkM1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     2-80: SSLSTGGGAG...RQLYSKAAAQ → NDSTDNLHAD...KAIIKSAEVR
     81-349: Missing.

Show »
Length:1,029
Mass (Da):111,765
Checksum:iD532D80FFFDAC461
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti175 – 177ESS → VSN in CAB57345 (PubMed:10485852).Curated3
Sequence conflicti188V → A in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti279Q → P in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti715A → G in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti715A → G in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti715A → G in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti746A → G in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti746A → G in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti746A → G in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti755A → G in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti755A → G in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti755A → G in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti797A → G in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti797A → G in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti797A → G in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti830R → G in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti830R → G in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti830R → G in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti970S → P in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti970S → P in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti970S → P in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti1029L → V in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti1029L → V in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti1029L → V in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti1134G → S in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti1134G → S in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti1134G → S in CAB99211 (PubMed:10485852).Curated1
Sequence conflicti1231E → K in CAB57344 (PubMed:10485852).Curated1
Sequence conflicti1231E → K in CAB57345 (PubMed:10485852).Curated1
Sequence conflicti1231E → K in CAB99211 (PubMed:10485852).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0524122 – 80SSLST…KAAAQ → NDSTDNLHADCDGRVSNNNN GNSNTNDGPNNDGDSDEEVI EGMALLEGNYQVLRQWVPPA PNYWDAPPKAIIKSAEVR in isoform B. 1 PublicationAdd BLAST79
Alternative sequenceiVSP_0524132 – 13SSLST…GAGGS → DTPNQMPVELER in isoform A. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_05241414 – 349Missing in isoform A. 1 PublicationAdd BLAST336
Alternative sequenceiVSP_05241581 – 349Missing in isoform B. 1 PublicationAdd BLAST269

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243708 mRNA. Translation: CAB57344.3.
AJ243710 mRNA. Translation: CAB57345.3.
AJ243709 mRNA. Translation: CAB99211.2.
AE013599 Genomic DNA. Translation: AAF59281.2.
AE013599 Genomic DNA. Translation: AAF59284.2.
AE013599 Genomic DNA. Translation: AAM68908.1.
RefSeqiNP_724534.1. NM_165508.2. [A1Z6W3-2]
NP_724535.1. NM_165509.2. [A1Z6W3-3]
NP_724538.1. NM_165512.2. [A1Z6W3-1]
UniGeneiDm.3470.

Genome annotation databases

EnsemblMetazoaiFBtr0089044; FBpp0088115; FBgn0003090. [A1Z6W3-1]
GeneIDi45343.
KEGGidme:Dmel_CG11084.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243708 mRNA. Translation: CAB57344.3.
AJ243710 mRNA. Translation: CAB57345.3.
AJ243709 mRNA. Translation: CAB99211.2.
AE013599 Genomic DNA. Translation: AAF59281.2.
AE013599 Genomic DNA. Translation: AAF59284.2.
AE013599 Genomic DNA. Translation: AAM68908.1.
RefSeqiNP_724534.1. NM_165508.2. [A1Z6W3-2]
NP_724535.1. NM_165509.2. [A1Z6W3-3]
NP_724538.1. NM_165512.2. [A1Z6W3-1]
UniGeneiDm.3470.

3D structure databases

ProteinModelPortaliA1Z6W3.
SMRiA1Z6W3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69608. 8 interactors.
DIPiDIP-59584N.
IntActiA1Z6W3. 2 interactors.
STRINGi7227.FBpp0088115.

Proteomic databases

PaxDbiA1Z6W3.
PRIDEiA1Z6W3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089044; FBpp0088115; FBgn0003090. [A1Z6W3-1]
GeneIDi45343.
KEGGidme:Dmel_CG11084.

Organism-specific databases

CTDi18745.
FlyBaseiFBgn0003090. pk.

Phylogenomic databases

eggNOGiKOG1704. Eukaryota.
ENOG410XP0W. LUCA.
GeneTreeiENSGT00550000074438.
InParanoidiA1Z6W3.
KOiK04511.
OMAiPKPMELQ.
OrthoDBiEOG091G07C5.
PhylomeDBiA1Z6W3.

Enzyme and pathway databases

ReactomeiR-DME-4608870. Asymmetric localization of PCP proteins.

Miscellaneous databases

ChiTaRSipk. fly.
GenomeRNAii45343.
PROiA1Z6W3.

Gene expression databases

BgeeiFBgn0003090.
ExpressionAtlasiA1Z6W3. baseline.
GenevisibleiA1Z6W3. DM.

Family and domain databases

CDDicd09415. LIM1_Prickle. 1 hit.
cd09418. LIM2_Prickle. 1 hit.
cd09420. LIM3_Prickle. 1 hit.
cd09827. PET_Prickle. 1 hit.
Gene3Di2.10.110.10. 3 hits.
InterProiIPR033725. LIM1_prickle.
IPR033726. LIM2_prickle.
IPR033727. LIM3_prickle.
IPR010442. PET_domain.
IPR033723. PET_prickle.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRIC1_DROME
AccessioniPrimary (citable) accession number: A1Z6W3
Secondary accession number(s): A1Z6V4
, A1Z6V8, Q9N9H6, Q9U5X0, Q9U5X1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: November 30, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.