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Protein

Protein gustavus

Gene

gus

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the localization of vas to the posterior pole of the oocyte. Required maternally in the germ line for efficient primordial germ cell formation.2 Publications

GO - Biological processi

  • cuticle pattern formation Source: UniProtKB
  • dorsal appendage formation Source: FlyBase
  • germ cell development Source: UniProtKB
  • intracellular signal transduction Source: InterPro
  • oocyte anterior/posterior axis specification Source: FlyBase
  • pole cell migration Source: UniProtKB
  • pole plasm assembly Source: UniProtKB
  • positive regulation of protein catabolic process Source: FlyBase
  • protein localization Source: UniProtKB
  • wing disc morphogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

ReactomeiR-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein gustavusImported
Gene namesi
Name:gus
ORF Names:CG2944
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0026238. gus.

Subcellular locationi

  • Cytoplasm 2 Publications
  • Nucleus 2 Publications

  • Note: Component of the cytoplasmic ribonucleoprotein (RNP) bodies which concentrate at the perinuclear region of the nurse cell and in punctate aggregates throughout the cytoplasm. Some cortical enrichment of these aggregates is also observed. At stage 7, accumulates in the anterior cytoplasm of the oocyte, whereas during stage 10 accumulates at the posterior pole. This posterior distribution is not maintained in later developmental stages.2 Publications

GO - Cellular componenti

  • cell cortex Source: UniProtKB
  • Cul5-RING ubiquitin ligase complex Source: FlyBase
  • cytoplasm Source: UniProtKB
  • elongin complex Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • pole plasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Homozygous females produce defective eggs, which desiccate and collapse soon after egg laying, or embryos, which hatch very rarely. Mutants exhibit wing morphology defects, including blistering, formation of ectopic vein material, and loss of material at the margin.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131Y → A: Does not affect binding to Elongin BC complex. Abolishes interaction with vas. 1 Publication1
Mutagenesisi147G → Y: Does not affect binding to Elongin BC complex. Abolishes interaction with vas. 1 Publication1
Mutagenesisi148R → W: Does not affect binding to Elongin BC complex. Abolishes interaction with vas. 1 Publication1
Mutagenesisi187E → A: Does not affect binding to Elongin BC complex or vas. 1 Publication1
Mutagenesisi204R → S: Does not affect binding to Elongin BC complex or vas. 1 Publication1
Mutagenesisi219W → L: Does not affect binding to Elongin BC complex. Decreases interaction with vas. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004221581 – 279Protein gustavusAdd BLAST279

Proteomic databases

PaxDbiA1Z6E0.
PRIDEiA1Z6E0.

Expressioni

Tissue specificityi

Expressed in ovaries, primarily in nurse cells and oocytes (at protein level).2 Publications

Developmental stagei

Expressed during oogenesis, in early embryos (0 to 4 hours) and in fertile adult females (at protein level).2 Publications

Gene expression databases

BgeeiFBgn0026238.
ExpressionAtlasiA1Z6E0. baseline.
GenevisibleiA1Z6E0. DM.

Interactioni

Subunit structurei

Interacts (via B30.2/SPRY domain) with vas; this interaction may be necessary for the transport of vas to the posterior pole of the oocyte. Interacts with Cul-5. May associate with the Elongin BC complex composed of Elongin-B and Elongin-C.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
stumpsO967573EBI-75338,EBI-74922
vasP090525EBI-75338,EBI-134067

Protein-protein interaction databases

BioGridi61396. 47 interactors.
IntActiA1Z6E0. 35 interactors.
STRINGi7227.FBpp0301582.

Structurei

Secondary structure

1279
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 44Combined sources8
Helixi50 – 55Combined sources6
Beta strandi57 – 63Combined sources7
Beta strandi67 – 70Combined sources4
Beta strandi73 – 79Combined sources7
Beta strandi85 – 92Combined sources8
Beta strandi97 – 105Combined sources9
Helixi108 – 110Combined sources3
Beta strandi116 – 120Combined sources5
Beta strandi126 – 131Combined sources6
Beta strandi141 – 145Combined sources5
Turni146 – 149Combined sources4
Beta strandi150 – 154Combined sources5
Turni155 – 157Combined sources3
Beta strandi161 – 164Combined sources4
Beta strandi177 – 184Combined sources8
Turni185 – 188Combined sources4
Beta strandi189 – 194Combined sources6
Beta strandi197 – 203Combined sources7
Beta strandi211 – 217Combined sources7
Beta strandi223 – 232Combined sources10
Helixi239 – 247Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FNJX-ray1.80A27-251[»]
2IHSX-ray2.20A/B27-232[»]
ProteinModelPortaliA1Z6E0.
SMRiA1Z6E0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA1Z6E0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 233B30.2/SPRYPROSITE-ProRule annotationAdd BLAST198
Domaini234 – 279SOCS boxPROSITE-ProRule annotationAdd BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 279Involved in binding to the Elongin BC complex1 PublicationAdd BLAST44

Sequence similaritiesi

Belongs to the SPSB family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 SOCS box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
InParanoidiA1Z6E0.
KOiK10343.
OMAiCKNQPGV.
OrthoDBiEOG091G0DF0.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001496. SOCS_box.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1Z6E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQKISGGVK TVSRNDSQST FKPIIPRELQ ADFVKPARID ILLDMPPASR
60 70 80 90 100
DLQLKHSWNS EDRSLNIFVK EDDKLTFHRH PVAQSTDCIR GKVGLTKGLH
110 120 130 140 150
IWEIYWPTRQ RGTHAVVGVC TADAPLHSVG YQSLVGSTEQ SWGWDLGRNK
160 170 180 190 200
LYHDSKNCAG VTYPAILKND EAFLVPDKFL VALDMDEGTL SFIVDQQYLG
210 220 230 240 250
IAFRGLRGKK LYPIVSAVWG HCEITMRYIG GLDPEPLPLM DLCRRTIRQK
260 270
IGRTNLEEHI QQLQLPLSMK TYLLYKNRR
Length:279
Mass (Da):31,713
Last modified:March 16, 2016 - v2
Checksum:iDCA657FEEA83D785
GO

Sequence cautioni

The sequence AAM50263 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence ACU12389 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence ACX32987 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence ADZ74173 differs from that shown. Reason: Frameshift at position 210.Curated
The sequence ADZ74173 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57346.2.
AE013599 Genomic DNA. Translation: AAF57347.3.
AE013599 Genomic DNA. Translation: AAG22337.2.
AE013599 Genomic DNA. Translation: AAM68372.1.
AE013599 Genomic DNA. Translation: AAM68373.2.
AE013599 Genomic DNA. Translation: AAM68374.2.
AY119609 mRNA. Translation: AAM50263.1. Different initiation.
BT089043 mRNA. Translation: ACU12389.1. Different initiation.
BT099916 mRNA. Translation: ACX32987.1. Different initiation.
BT126146 mRNA. Translation: ADZ74173.1. Sequence problems.
RefSeqiNP_001246140.1. NM_001259211.2.
NP_610158.3. NM_136314.4.
NP_724398.2. NM_165418.3.
NP_724399.2. NM_165419.3.
NP_724400.2. NM_165420.3.
NP_724401.2. NM_165421.3.
NP_724402.2. NM_165422.3.
UniGeneiDm.7323.

Genome annotation databases

EnsemblMetazoaiFBtr0309846; FBpp0301580; FBgn0026238.
FBtr0309847; FBpp0301581; FBgn0026238.
FBtr0309848; FBpp0301582; FBgn0026238.
FBtr0309849; FBpp0301583; FBgn0026238.
FBtr0309850; FBpp0301584; FBgn0026238.
FBtr0309851; FBpp0301585; FBgn0026238.
FBtr0309852; FBpp0301586; FBgn0026238.
GeneIDi35478.
KEGGidme:Dmel_CG2944.
UCSCiCG2944-RA. d. melanogaster.
CG2944-RB. d. melanogaster.
CG2944-RD. d. melanogaster.
CG2944-RE. d. melanogaster.
CG2944-RF. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57346.2.
AE013599 Genomic DNA. Translation: AAF57347.3.
AE013599 Genomic DNA. Translation: AAG22337.2.
AE013599 Genomic DNA. Translation: AAM68372.1.
AE013599 Genomic DNA. Translation: AAM68373.2.
AE013599 Genomic DNA. Translation: AAM68374.2.
AY119609 mRNA. Translation: AAM50263.1. Different initiation.
BT089043 mRNA. Translation: ACU12389.1. Different initiation.
BT099916 mRNA. Translation: ACX32987.1. Different initiation.
BT126146 mRNA. Translation: ADZ74173.1. Sequence problems.
RefSeqiNP_001246140.1. NM_001259211.2.
NP_610158.3. NM_136314.4.
NP_724398.2. NM_165418.3.
NP_724399.2. NM_165419.3.
NP_724400.2. NM_165420.3.
NP_724401.2. NM_165421.3.
NP_724402.2. NM_165422.3.
UniGeneiDm.7323.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FNJX-ray1.80A27-251[»]
2IHSX-ray2.20A/B27-232[»]
ProteinModelPortaliA1Z6E0.
SMRiA1Z6E0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61396. 47 interactors.
IntActiA1Z6E0. 35 interactors.
STRINGi7227.FBpp0301582.

Proteomic databases

PaxDbiA1Z6E0.
PRIDEiA1Z6E0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0309846; FBpp0301580; FBgn0026238.
FBtr0309847; FBpp0301581; FBgn0026238.
FBtr0309848; FBpp0301582; FBgn0026238.
FBtr0309849; FBpp0301583; FBgn0026238.
FBtr0309850; FBpp0301584; FBgn0026238.
FBtr0309851; FBpp0301585; FBgn0026238.
FBtr0309852; FBpp0301586; FBgn0026238.
GeneIDi35478.
KEGGidme:Dmel_CG2944.
UCSCiCG2944-RA. d. melanogaster.
CG2944-RB. d. melanogaster.
CG2944-RD. d. melanogaster.
CG2944-RE. d. melanogaster.
CG2944-RF. d. melanogaster.

Organism-specific databases

CTDi35478.
FlyBaseiFBgn0026238. gus.

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
InParanoidiA1Z6E0.
KOiK10343.
OMAiCKNQPGV.
OrthoDBiEOG091G0DF0.

Enzyme and pathway databases

ReactomeiR-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTraceiA1Z6E0.
GenomeRNAii35478.
PROiA1Z6E0.

Gene expression databases

BgeeiFBgn0026238.
ExpressionAtlasiA1Z6E0. baseline.
GenevisibleiA1Z6E0. DM.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001496. SOCS_box.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF07525. SOCS_box. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00969. SOCS_box. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50225. SOCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUS_DROME
AccessioniPrimary (citable) accession number: A1Z6E0
Secondary accession number(s): A1Z6D7
, A1Z6D9, A1Z6E2, F2FB59, Q7JUY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: March 16, 2016
Last modified: November 30, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.