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A1YYW7

- ALPH_SPHSX

UniProt

A1YYW7 - ALPH_SPHSX

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Protein
Alkaline phosphatase PhoK
Gene
phoK
Organism
Sphingomonas sp.
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Precipitates uranium from alkaline solutions.2 Publications

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Binds 2 zinc ions.1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491Zinc 1
Active sitei89 – 891Phosphothreonine intermediate Inferred
Metal bindingi89 – 891Zinc 1
Binding sitei110 – 1101Substrate
Metal bindingi300 – 3001Zinc 2
Metal bindingi304 – 3041Zinc 2; via tele nitrogen
Metal bindingi345 – 3451Zinc 1
Metal bindingi346 – 3461Zinc 1; via tele nitrogen
Metal bindingi491 – 4911Zinc 2; via tele nitrogen

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase PhoK (EC:3.1.3.1)
Alternative name(s):
SPAP protein
Gene namesi
Name:phoK
OrganismiSphingomonas sp.
Taxonomic identifieri28214 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 559540Alkaline phosphatase PhoK
PRO_0000392472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi90 ↔ 1261 Publication
Disulfide bondi231 ↔ 3141 Publication
Disulfide bondi545 ↔ 5561 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi41 – 488
Helixi53 – 597
Helixi60 – 623
Helixi66 – 738
Beta strandi74 – 818
Helixi89 – 968
Helixi102 – 1054
Beta strandi109 – 1135
Beta strandi118 – 1203
Beta strandi123 – 1253
Helixi152 – 1598
Beta strandi165 – 1717
Helixi172 – 1798
Beta strandi184 – 1896
Helixi205 – 21814
Helixi229 – 2346
Beta strandi238 – 2403
Beta strandi243 – 2464
Helixi257 – 2615
Helixi264 – 28017
Turni281 – 2844
Beta strandi285 – 2884
Beta strandi290 – 2967
Helixi298 – 3069
Beta strandi308 – 3103
Helixi311 – 33323
Beta strandi338 – 3436
Helixi352 – 3554
Turni356 – 3594
Helixi368 – 3703
Helixi372 – 38312
Beta strandi391 – 40212
Helixi408 – 42417
Beta strandi428 – 4336
Helixi434 – 4385
Helixi447 – 4493
Helixi452 – 4587
Turni462 – 4643
Beta strandi467 – 4726
Beta strandi476 – 4794
Helixi496 – 4994
Beta strandi500 – 5078
Helixi521 – 5233
Helixi524 – 5318
Helixi538 – 5403

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3Q3QX-ray1.95A1-559[»]

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni171 – 1733Substrate binding

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1YYW7-1 [UniParc]FASTAAdd to Basket

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MLKHVAAALL LATAMPVVAQ SPAPAAAPAP AARSIAATPP KLIVAISVDQ    50
FSADLFSEYR QYYTGGLKRL TSEGAVFPRG YQSHAATETC PGHSTILTGS 100
RPSRTGIIAN NWFDLDAKRE DKNLYCAEDE SQPGSSSDKY EASPLHLKVP 150
TLGGRMKAAN PATRVVSVAG KDRAAIMMGG ATADQVWWLG GPQGYVSYKG 200
VAPTPLVTQV NQAFAQRLAQ PNPGFELPAQ CVSKDFPVQA GNRTVGTGRF 250
ARDAGDYKGF RISPEQDAMT LAFAAAAIEN MQLGKQAQTD IISIGLSATD 300
YVGHTFGTEG TESCIQVDRL DTELGAFFDK LDKDGIDYVV VLTADHGGHD 350
LPERHRMNAM PMEQRVDMAL TPKALNATIA EKAGLPGKKV IWSDGPSGDI 400
YYDKGLTAAQ RARVETEALK YLRAHPQVQT VFTKAEIAAT PSPSGPPESW 450
SLIQEARASF YPSRSGDLLL LLKPRVMSIP EQAVMGSVAT HGSPWDTDRR 500
VPILFWRKGM QHFEQPLGVE TVDILPSLAA LIKLPVPKDQ IDGRCLDLVA 550
GKDDSCAGQ 559
Length:559
Mass (Da):59,982
Last modified:February 6, 2007 - v1
Checksum:i0A7A0F7838E1A186
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF143994 Genomic DNA. Translation: ABL96598.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF143994 Genomic DNA. Translation: ABL96598.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3Q3Q X-ray 1.95 A 1-559 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.720.10. 2 hits.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view ]
Pfami PF01663. Phosphodiest. 1 hit.
[Graphical view ]
PIRSFi PIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMi SSF53649. SSF53649. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Cloning and overexpression of alkaline phosphatase PhoK from Sphingomonas sp. strain BSAR-1 for bioprecipitation of uranium from alkaline solutions."
    Nilgiriwala K.S., Alahari A., Rao A.S., Apte S.K.
    Appl. Environ. Microbiol. 74:5516-5523(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
    Strain: BSAR-1.
  2. "PhoK alkaline phosphatase."
    Nilgiriwala K.S., Apte S.K.
    Submitted (SEP-2009) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: BSAR-1.
  3. "Crystallization and preliminary X-ray crystallographic analysis of PhoK, an extracellular alkaline phosphatase from Sphingomonas sp. BSAR-1."
    Nilgiriwala K.S., Bihani S.C., Das A., Prashar V., Kumar M., Ferrer J.L., Apte S.K., Hosur M.V.
    Acta Crystallogr. F 65:917-919(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Strain: BSAR-1.
  4. "X-ray structure reveals a new class and provides insight into evolution of alkaline phosphatases."
    Bihani S.C., Das A., Nilgiriwala K.S., Prashar V., Pirocchi M., Apte S.K., Ferrer J.L., Hosur M.V.
    PLoS ONE 6:E22767-E22767(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH ZINC AND SUBSTRATE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, ACTIVE SITE, DISULFIDE BONDS.
    Strain: BSAR-1.

Entry informationi

Entry nameiALPH_SPHSX
AccessioniPrimary (citable) accession number: A1YYW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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