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Protein

Alkaline phosphatase PhoK

Gene

phoK

Organism
Sphingomonas sp.
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Precipitates uranium from alkaline solutions.2 Publications

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions.1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi49Zinc 11 Publication1
Active sitei89Phosphothreonine intermediate1 Publication1
Metal bindingi89Zinc 11 Publication1
Binding sitei110Substrate1 Publication1
Metal bindingi300Zinc 21 Publication1
Metal bindingi304Zinc 2; via tele nitrogen1 Publication1
Metal bindingi345Zinc 11 Publication1
Metal bindingi346Zinc 1; via tele nitrogen1 Publication1
Metal bindingi491Zinc 2; via tele nitrogen1 Publication1

GO - Molecular functioni

  • alkaline phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1. 10569.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase PhoK (EC:3.1.3.1)
Alternative name(s):
SPAP protein
Gene namesi
Name:phoKImported
OrganismiSphingomonas sp.
Taxonomic identifieri28214 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000039247220 – 559Alkaline phosphatase PhoKAdd BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi90 ↔ 1261 Publication
Disulfide bondi231 ↔ 3141 Publication
Disulfide bondi545 ↔ 5561 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1559
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 48Combined sources8
Helixi53 – 59Combined sources7
Helixi60 – 62Combined sources3
Helixi66 – 73Combined sources8
Beta strandi74 – 81Combined sources8
Helixi89 – 96Combined sources8
Helixi102 – 105Combined sources4
Beta strandi109 – 113Combined sources5
Beta strandi118 – 120Combined sources3
Beta strandi123 – 125Combined sources3
Helixi152 – 159Combined sources8
Beta strandi165 – 171Combined sources7
Helixi172 – 179Combined sources8
Beta strandi184 – 189Combined sources6
Helixi205 – 218Combined sources14
Helixi229 – 234Combined sources6
Beta strandi238 – 240Combined sources3
Beta strandi243 – 246Combined sources4
Helixi257 – 261Combined sources5
Helixi264 – 280Combined sources17
Turni281 – 284Combined sources4
Beta strandi285 – 288Combined sources4
Beta strandi290 – 296Combined sources7
Helixi298 – 306Combined sources9
Beta strandi308 – 310Combined sources3
Helixi311 – 333Combined sources23
Beta strandi338 – 343Combined sources6
Helixi352 – 355Combined sources4
Turni356 – 359Combined sources4
Helixi368 – 370Combined sources3
Helixi372 – 383Combined sources12
Beta strandi391 – 402Combined sources12
Helixi408 – 424Combined sources17
Beta strandi428 – 433Combined sources6
Helixi434 – 438Combined sources5
Helixi447 – 449Combined sources3
Helixi452 – 458Combined sources7
Turni462 – 464Combined sources3
Beta strandi467 – 472Combined sources6
Beta strandi476 – 479Combined sources4
Helixi496 – 499Combined sources4
Beta strandi500 – 507Combined sources8
Helixi521 – 523Combined sources3
Helixi524 – 531Combined sources8
Helixi538 – 540Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3Q3QX-ray1.95A1-559[»]
SMRiA1YYW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni171 – 173Substrate binding3

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1YYW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKHVAAALL LATAMPVVAQ SPAPAAAPAP AARSIAATPP KLIVAISVDQ
60 70 80 90 100
FSADLFSEYR QYYTGGLKRL TSEGAVFPRG YQSHAATETC PGHSTILTGS
110 120 130 140 150
RPSRTGIIAN NWFDLDAKRE DKNLYCAEDE SQPGSSSDKY EASPLHLKVP
160 170 180 190 200
TLGGRMKAAN PATRVVSVAG KDRAAIMMGG ATADQVWWLG GPQGYVSYKG
210 220 230 240 250
VAPTPLVTQV NQAFAQRLAQ PNPGFELPAQ CVSKDFPVQA GNRTVGTGRF
260 270 280 290 300
ARDAGDYKGF RISPEQDAMT LAFAAAAIEN MQLGKQAQTD IISIGLSATD
310 320 330 340 350
YVGHTFGTEG TESCIQVDRL DTELGAFFDK LDKDGIDYVV VLTADHGGHD
360 370 380 390 400
LPERHRMNAM PMEQRVDMAL TPKALNATIA EKAGLPGKKV IWSDGPSGDI
410 420 430 440 450
YYDKGLTAAQ RARVETEALK YLRAHPQVQT VFTKAEIAAT PSPSGPPESW
460 470 480 490 500
SLIQEARASF YPSRSGDLLL LLKPRVMSIP EQAVMGSVAT HGSPWDTDRR
510 520 530 540 550
VPILFWRKGM QHFEQPLGVE TVDILPSLAA LIKLPVPKDQ IDGRCLDLVA

GKDDSCAGQ
Length:559
Mass (Da):59,982
Last modified:February 6, 2007 - v1
Checksum:i0A7A0F7838E1A186
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF143994 Genomic DNA. Translation: ABL96598.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF143994 Genomic DNA. Translation: ABL96598.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3Q3QX-ray1.95A1-559[»]
SMRiA1YYW7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.3.1. 10569.

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiALPH_SPHSX
AccessioniPrimary (citable) accession number: A1YYW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.