A1YGR6 (GGTA1_LORTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyllactosaminide alpha-1,3-galactosyltransferase
EC=2.4.1.87

Alternative name(s):
UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase
Short name=Galactosyltransferase

Gene names

Name:

GGTA1

Organism

Loris tardigradus (Slender loris)

Taxonomic identifier

9468 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Strepsirrhini › Chiromyiformes › Lorisidae › Loris

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R).
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis By similarity.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyllactosaminide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	N-acetyllactosaminide alpha-1,3-galactosyltransferase		PRO_0000333703
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 25	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 376	351	Lumenal Potential
Sites
	Metal binding	233	1	Manganese By similarity
	Metal binding	235	1	Manganese By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1YGR6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 00BB04B9DEF629F4	FASTA	376	44,381
	10 20 30 40 50 60 MNVKGKAILS MLVASTVIVV FWEYINSSEG SFLWIYHSKN PEVGDVRAPM GWWFPSWFNN 70 80 90 100 110 120 GTHIYQEEEE DVDKEKGRKK EQREKDDREE LQLWDWFTPE KRPEVVTVTS WKAPVVWEGT 130 140 150 160 170 180 YNSAILENYY AKQKITVGLT VFAIGKYLEY YLEEFIASAD RYFMVGHKVI FYIMVNNVSR 190 200 210 220 230 240 MPPLELGPLR SFEVFEIKAE KRWQDVSMMR MKIIGEHILT HIQHEVDFLF CMDVDQVFQD 250 260 270 280 290 300 NFGVETLGES VAQLQAWWYK ADPNEFTYER REKSAAYIPF GQGDFYYHAA IFGGTPIRVL 310 320 330 340 350 360 NITQECFKGI LQDKKNDIEA NWHDESHLNK YFLVNKPSKI LSPEYCWDYQ IGLPSDIKIV 370 KISWQTKEYH LVRNNV

References

[1]	"Functionally important glycosyltransferase gain and loss during catarrhine primate emergence." Koike C., Uddin M., Wildman D.E., Gray E.A., Trucco M., Starzl T.E., Goodman M. Proc. Natl. Acad. Sci. U.S.A. 104:559-564(2007) [PubMed: 17194757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ985357 mRNA. Translation: ABL85466.1.
3D structure databases
ProteinModelPortal	A1YGR6.
SMR	A1YGR6. Positions 90-376.
ModBase	Search...
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG003563.
Enzyme and pathway databases
BRENDA	2.4.1.87. 3073.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGTA1_LORTA

Accession

Primary (citable) accession number: A1YGR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	May 31, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program