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A1Y9I9

- TOMT_MOUSE

UniProt

A1Y9I9 - TOMT_MOUSE

Protein

Transmembrane O-methyltransferase

Gene

Tomt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones By similarity. Required for auditory function.By similarity1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei112 – 1121S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei130 – 1301S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei160 – 1601S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation

    GO - Molecular functioni

    1. catechol O-methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. auditory receptor cell development Source: UniProtKB
    2. catecholamine catabolic process Source: UniProtKB
    3. neurotransmitter catabolic process Source: UniProtKB-KW
    4. sensory perception of sound Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Catecholamine metabolism, Hearing, Neurotransmitter degradation

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    SABIO-RKA1Y9I9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transmembrane O-methyltransferase (EC:2.1.1.6)
    Alternative name(s):
    Catechol O-methyltransferase 21 Publication
    Protein LRTOMT2
    Gene namesi
    Name:Tomt
    Synonyms:Comt21 Publication
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:3769724. Tomt.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi48 – 481R → L in add; reduces methyltransferase activity; causes hyperkinesis, circling, head-tossing, aggression, progressive degeneration of the organ of Corti, hair cell defects and profound deafness. 1 Publication

    Keywords - Diseasei

    Deafness

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 258258Transmembrane O-methyltransferasePRO_0000354094Add
    BLAST

    Proteomic databases

    PRIDEiA1Y9I9.

    Expressioni

    Tissue specificityi

    Widely expressed with high levels in outer and inner hair cells of the cochlea and vestibule.2 Publications

    Developmental stagei

    Not detected in the embryo at E12.5. At E14.5, expressed in the developing inner ear. At E16.5, expressed in the utricle and saccule. At E18.5, expressed specifically in the region of the sensory cells of the cochlea, utricle, saccule and crista ampullaris.1 Publication

    Gene expression databases

    BgeeiA1Y9I9.
    GenevestigatoriA1Y9I9.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000102582.

    Structurei

    3D structure databases

    ProteinModelPortaliA1Y9I9.
    SMRiA1Y9I9. Positions 46-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 1072S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4122.
    GeneTreeiENSGT00390000011316.
    HOGENOMiHOG000046392.
    InParanoidiA1Y9I9.
    OMAiIPCLRTQ.
    OrthoDBiEOG77T154.
    PhylomeDBiA1Y9I9.
    TreeFamiTF329140.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025782. Catechol_O-MeTrfase.
    IPR002935. O-MeTrfase_3.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10509. PTHR10509. 1 hit.
    PfamiPF01596. Methyltransf_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51682. SAM_OMT_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1Y9I9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPAIALAFL PLVVTLLVRY RHHFRLLVRT VLLRGFRDCL SGLRIEERAF    50
    SYVLTHALPG DPGHILTTLD HWSSCCEYLS HMGPVKGQIL MRLVEEKAPA 100
    CVLELGTYCG YSTLLIARAL PPGSRLLTVE RDSRTAAVAE KVIRLAGFDE 150
    QMVELIAGSS EEVIPRLRAQ HQLNRADLVL LAHRPRYYLR DLQLLEAHAL 200
    LPHGATVLAD HVLFPGAPRF LQYTKSCGRY RCRLHHTSLP DFPAIKDGIA 250
    QLTYTGPG 258
    Length:258
    Mass (Da):28,846
    Last modified:February 6, 2007 - v1
    Checksum:i393954F017C67E0C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU627091 mRNA. Translation: ACF40901.1.
    DQ854743 mRNA. Translation: ABI37014.1.
    CCDSiCCDS40044.1.
    RefSeqiNP_001075148.1. NM_001081679.1.
    NP_001269017.1. NM_001282088.1.
    XP_006508413.1. XM_006508350.1.
    UniGeneiMm.475469.

    Genome annotation databases

    EnsembliENSMUST00000106969; ENSMUSP00000102582; ENSMUSG00000078630.
    ENSMUST00000106970; ENSMUSP00000102583; ENSMUSG00000078630.
    GeneIDi791260.
    KEGGimmu:791260.
    UCSCiuc009ipu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU627091 mRNA. Translation: ACF40901.1 .
    DQ854743 mRNA. Translation: ABI37014.1 .
    CCDSi CCDS40044.1.
    RefSeqi NP_001075148.1. NM_001081679.1.
    NP_001269017.1. NM_001282088.1.
    XP_006508413.1. XM_006508350.1.
    UniGenei Mm.475469.

    3D structure databases

    ProteinModelPortali A1Y9I9.
    SMRi A1Y9I9. Positions 46-257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000102582.

    Proteomic databases

    PRIDEi A1Y9I9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000106969 ; ENSMUSP00000102582 ; ENSMUSG00000078630 .
    ENSMUST00000106970 ; ENSMUSP00000102583 ; ENSMUSG00000078630 .
    GeneIDi 791260.
    KEGGi mmu:791260.
    UCSCi uc009ipu.1. mouse.

    Organism-specific databases

    CTDi 791260.
    MGIi MGI:3769724. Tomt.

    Phylogenomic databases

    eggNOGi COG4122.
    GeneTreei ENSGT00390000011316.
    HOGENOMi HOG000046392.
    InParanoidi A1Y9I9.
    OMAi IPCLRTQ.
    OrthoDBi EOG77T154.
    PhylomeDBi A1Y9I9.
    TreeFami TF329140.

    Enzyme and pathway databases

    SABIO-RK A1Y9I9.

    Miscellaneous databases

    ChiTaRSi LRTOMT. mouse.
    NextBioi 440083.
    PROi A1Y9I9.
    SOURCEi Search...

    Gene expression databases

    Bgeei A1Y9I9.
    Genevestigatori A1Y9I9.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR025782. Catechol_O-MeTrfase.
    IPR002935. O-MeTrfase_3.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10509. PTHR10509. 1 hit.
    Pfami PF01596. Methyltransf_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51682. SAM_OMT_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6J.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-48.
      Strain: C57BL/6JImported.
      Tissue: BrainImported.

    Entry informationi

    Entry nameiTOMT_MOUSE
    AccessioniPrimary (citable) accession number: A1Y9I9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene.

    Caution

    Despite its name, the murine TOMT protein does not contain a transmembrane region in contrast to primate orthologs.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3