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Protein

Transmembrane O-methyltransferase

Gene

Tomt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones (By similarity). Required for auditory function.By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei112 – 1121S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei130 – 1301S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei160 – 1601S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation

GO - Molecular functioni

  1. catechol O-methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. auditory receptor cell development Source: UniProtKB
  2. catecholamine catabolic process Source: UniProtKB
  3. neurotransmitter catabolic process Source: UniProtKB-KW
  4. sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Hearing, Neurotransmitter degradation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKA1Y9I9.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane O-methyltransferase (EC:2.1.1.6)
Alternative name(s):
Catechol O-methyltransferase 21 Publication
Protein LRTOMT2
Gene namesi
Name:Tomt
Synonyms:Comt21 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:3769724. Tomt.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481R → L in add; reduces methyltransferase activity; causes hyperkinesis, circling, head-tossing, aggression, progressive degeneration of the organ of Corti, hair cell defects and profound deafness. 1 Publication

Keywords - Diseasei

Deafness

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Transmembrane O-methyltransferasePRO_0000354094Add
BLAST

Proteomic databases

PRIDEiA1Y9I9.

Expressioni

Tissue specificityi

Widely expressed with high levels in outer and inner hair cells of the cochlea and vestibule.2 Publications

Developmental stagei

Not detected in the embryo at E12.5. At E14.5, expressed in the developing inner ear. At E16.5, expressed in the utricle and saccule. At E18.5, expressed specifically in the region of the sensory cells of the cochlea, utricle, saccule and crista ampullaris.1 Publication

Gene expression databases

BgeeiA1Y9I9.
GenevestigatoriA1Y9I9.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102582.

Structurei

3D structure databases

ProteinModelPortaliA1Y9I9.
SMRiA1Y9I9. Positions 46-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni106 – 1072S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4122.
GeneTreeiENSGT00390000011316.
HOGENOMiHOG000046392.
InParanoidiA1Y9I9.
OMAiLDFVFMD.
OrthoDBiEOG77T154.
PhylomeDBiA1Y9I9.
TreeFamiTF329140.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1Y9I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPAIALAFL PLVVTLLVRY RHHFRLLVRT VLLRGFRDCL SGLRIEERAF
60 70 80 90 100
SYVLTHALPG DPGHILTTLD HWSSCCEYLS HMGPVKGQIL MRLVEEKAPA
110 120 130 140 150
CVLELGTYCG YSTLLIARAL PPGSRLLTVE RDSRTAAVAE KVIRLAGFDE
160 170 180 190 200
QMVELIAGSS EEVIPRLRAQ HQLNRADLVL LAHRPRYYLR DLQLLEAHAL
210 220 230 240 250
LPHGATVLAD HVLFPGAPRF LQYTKSCGRY RCRLHHTSLP DFPAIKDGIA

QLTYTGPG
Length:258
Mass (Da):28,846
Last modified:February 6, 2007 - v1
Checksum:i393954F017C67E0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU627091 mRNA. Translation: ACF40901.1.
DQ854743 mRNA. Translation: ABI37014.1.
CCDSiCCDS40044.1.
RefSeqiNP_001075148.1. NM_001081679.1.
NP_001269017.1. NM_001282088.1.
XP_006508413.1. XM_006508350.1.
UniGeneiMm.475469.

Genome annotation databases

EnsembliENSMUST00000106969; ENSMUSP00000102582; ENSMUSG00000078630.
ENSMUST00000106970; ENSMUSP00000102583; ENSMUSG00000078630.
GeneIDi791260.
KEGGimmu:791260.
UCSCiuc009ipu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU627091 mRNA. Translation: ACF40901.1.
DQ854743 mRNA. Translation: ABI37014.1.
CCDSiCCDS40044.1.
RefSeqiNP_001075148.1. NM_001081679.1.
NP_001269017.1. NM_001282088.1.
XP_006508413.1. XM_006508350.1.
UniGeneiMm.475469.

3D structure databases

ProteinModelPortaliA1Y9I9.
SMRiA1Y9I9. Positions 46-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102582.

Proteomic databases

PRIDEiA1Y9I9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106969; ENSMUSP00000102582; ENSMUSG00000078630.
ENSMUST00000106970; ENSMUSP00000102583; ENSMUSG00000078630.
GeneIDi791260.
KEGGimmu:791260.
UCSCiuc009ipu.1. mouse.

Organism-specific databases

CTDi791260.
MGIiMGI:3769724. Tomt.

Phylogenomic databases

eggNOGiCOG4122.
GeneTreeiENSGT00390000011316.
HOGENOMiHOG000046392.
InParanoidiA1Y9I9.
OMAiLDFVFMD.
OrthoDBiEOG77T154.
PhylomeDBiA1Y9I9.
TreeFamiTF329140.

Enzyme and pathway databases

SABIO-RKA1Y9I9.

Miscellaneous databases

NextBioi440083.
PROiA1Y9I9.
SOURCEiSearch...

Gene expression databases

BgeeiA1Y9I9.
GenevestigatoriA1Y9I9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-48.
    Strain: C57BL/6JImported.
    Tissue: BrainImported.

Entry informationi

Entry nameiTOMT_MOUSE
AccessioniPrimary (citable) accession number: A1Y9I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: February 6, 2007
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene.

Caution

Despite its name, the murine TOMT protein does not contain a transmembrane region in contrast to primate orthologs.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.