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A1Y9I9

- TOMT_MOUSE

UniProt

A1Y9I9 - TOMT_MOUSE

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Protein

Transmembrane O-methyltransferase

Gene
Tomt, Comt2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones By similarity. Required for auditory function.By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041S-adenosyl-L-methionine By similarity
Binding sitei112 – 1121S-adenosyl-L-methionine By similarity
Binding sitei130 – 1301S-adenosyl-L-methionine By similarity
Binding sitei160 – 1601S-adenosyl-L-methionine; via amide nitrogen By similarity

GO - Molecular functioni

  1. catechol O-methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. auditory receptor cell development Source: UniProtKB
  2. catecholamine catabolic process Source: UniProtKB
  3. neurotransmitter catabolic process Source: UniProtKB-KW
  4. sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Hearing, Neurotransmitter degradation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKA1Y9I9.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane O-methyltransferase (EC:2.1.1.6)
Alternative name(s):
Catechol O-methyltransferase 2
Protein LRTOMT2
Gene namesi
Name:Tomt
Synonyms:Comt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:3769724. Tomt.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481R → L in add; reduces methyltransferase activity; causes hyperkinesis, circling, head-tossing, aggression, progressive degeneration of the organ of Corti, hair cell defects and profound deafness. 1 Publication

Keywords - Diseasei

Deafness

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Transmembrane O-methyltransferasePRO_0000354094Add
BLAST

Proteomic databases

PRIDEiA1Y9I9.

Expressioni

Tissue specificityi

Widely expressed with high levels in outer and inner hair cells of the cochlea and vestibule.2 Publications

Developmental stagei

Not detected in the embryo at E12.5. At E14.5, expressed in the developing inner ear. At E16.5, expressed in the utricle and saccule. At E18.5, expressed specifically in the region of the sensory cells of the cochlea, utricle, saccule and crista ampullaris.1 Publication

Gene expression databases

BgeeiA1Y9I9.
GenevestigatoriA1Y9I9.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102582.

Structurei

3D structure databases

ProteinModelPortaliA1Y9I9.
SMRiA1Y9I9. Positions 46-257.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni106 – 1072S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4122.
GeneTreeiENSGT00390000011316.
HOGENOMiHOG000046392.
InParanoidiA1Y9I9.
OMAiIPCLRTQ.
OrthoDBiEOG77T154.
PhylomeDBiA1Y9I9.
TreeFamiTF329140.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025782. Catechol_O-MeTrfase.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1Y9I9-1 [UniParc]FASTAAdd to Basket

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MSPAIALAFL PLVVTLLVRY RHHFRLLVRT VLLRGFRDCL SGLRIEERAF    50
SYVLTHALPG DPGHILTTLD HWSSCCEYLS HMGPVKGQIL MRLVEEKAPA 100
CVLELGTYCG YSTLLIARAL PPGSRLLTVE RDSRTAAVAE KVIRLAGFDE 150
QMVELIAGSS EEVIPRLRAQ HQLNRADLVL LAHRPRYYLR DLQLLEAHAL 200
LPHGATVLAD HVLFPGAPRF LQYTKSCGRY RCRLHHTSLP DFPAIKDGIA 250
QLTYTGPG 258
Length:258
Mass (Da):28,846
Last modified:February 6, 2007 - v1
Checksum:i393954F017C67E0C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU627091 mRNA. Translation: ACF40901.1.
DQ854743 mRNA. Translation: ABI37014.1.
CCDSiCCDS40044.1.
RefSeqiNP_001075148.1. NM_001081679.1.
NP_001269017.1. NM_001282088.1.
XP_006508413.1. XM_006508350.1.
UniGeneiMm.475469.

Genome annotation databases

EnsembliENSMUST00000106969; ENSMUSP00000102582; ENSMUSG00000078630.
ENSMUST00000106970; ENSMUSP00000102583; ENSMUSG00000078630.
GeneIDi791260.
KEGGimmu:791260.
UCSCiuc009ipu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU627091 mRNA. Translation: ACF40901.1 .
DQ854743 mRNA. Translation: ABI37014.1 .
CCDSi CCDS40044.1.
RefSeqi NP_001075148.1. NM_001081679.1.
NP_001269017.1. NM_001282088.1.
XP_006508413.1. XM_006508350.1.
UniGenei Mm.475469.

3D structure databases

ProteinModelPortali A1Y9I9.
SMRi A1Y9I9. Positions 46-257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000102582.

Proteomic databases

PRIDEi A1Y9I9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000106969 ; ENSMUSP00000102582 ; ENSMUSG00000078630 .
ENSMUST00000106970 ; ENSMUSP00000102583 ; ENSMUSG00000078630 .
GeneIDi 791260.
KEGGi mmu:791260.
UCSCi uc009ipu.1. mouse.

Organism-specific databases

CTDi 791260.
MGIi MGI:3769724. Tomt.

Phylogenomic databases

eggNOGi COG4122.
GeneTreei ENSGT00390000011316.
HOGENOMi HOG000046392.
InParanoidi A1Y9I9.
OMAi IPCLRTQ.
OrthoDBi EOG77T154.
PhylomeDBi A1Y9I9.
TreeFami TF329140.

Enzyme and pathway databases

SABIO-RK A1Y9I9.

Miscellaneous databases

ChiTaRSi LRTOMT. mouse.
NextBioi 440083.
PROi A1Y9I9.
SOURCEi Search...

Gene expression databases

Bgeei A1Y9I9.
Genevestigatori A1Y9I9.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR025782. Catechol_O-MeTrfase.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10509. PTHR10509. 1 hit.
Pfami PF01596. Methyltransf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51682. SAM_OMT_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-48.
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiTOMT_MOUSE
AccessioniPrimary (citable) accession number: A1Y9I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: February 6, 2007
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene.

Caution

Despite its name, the murine TOMT protein does not contain a transmembrane region in contrast to primate orthologs.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi