Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A1Y2K1

- FYN_PIG

UniProt

A1Y2K1 - FYN_PIG

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Tyrosine-protein kinase Fyn

Gene

FYN

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1 (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.By similarityPROSITE-ProRule annotation

Cofactori

Manganese.By similarity

Enzyme regulationi

Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei299 – 2991ATPBy similarityPROSITE-ProRule annotation
Active sitei390 – 3901Proton acceptorBy similarityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi277 – 2859ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. activated T cell proliferation Source: Ensembl
  2. dendrite morphogenesis Source: Ensembl
  3. detection of mechanical stimulus involved in sensory perception of pain Source: Ensembl
  4. forebrain development Source: Ensembl
  5. negative regulation of gene expression Source: Ensembl
  6. negative regulation of protein catabolic process Source: Ensembl
  7. neuron migration Source: Ensembl
  8. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  9. positive regulation of neuron projection development Source: Ensembl
  10. positive regulation of protein localization to nucleus Source: Ensembl
  11. protein autophosphorylation Source: Ensembl
  12. regulation of cell shape Source: Ensembl
  13. response to ethanol Source: Ensembl
  14. T cell receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Adaptive immunity, Immunity

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_192916. Signaling by SCF-KIT.
REACT_193226. DAP12 signaling.
REACT_202079. Sema3A PAK dependent Axon repulsion.
REACT_209088. CD28 dependent PI3K/Akt signaling.
REACT_210728. Regulation of KIT signaling.
REACT_210856. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.
REACT_212538. Role of LAT2/NTAL/LAB on calcium mobilization.
REACT_213066. Nephrin interactions.
REACT_214206. CTLA4 inhibitory signaling.
REACT_214362. Regulation of signaling by CBL.
REACT_217307. Netrin mediated repulsion signals.
REACT_218737. CRMPs in Sema3A signaling.
REACT_219289. PIP3 activates AKT signaling.
REACT_223200. Platelet Adhesion to exposed collagen.
REACT_223707. CD28 co-stimulation.
REACT_224799. Constitutive PI3K/AKT Signaling in Cancer.
REACT_226043. DCC mediated attractive signaling.
REACT_226779. CD28 dependent Vav1 pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Fyn (EC:2.7.10.2)
Alternative name(s):
Proto-oncogene c-Fyn
p59-Fyn
Gene namesi
Name:FYNImported
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 1

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Cell membrane By similarity
Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking (By similarity).By similarity

GO - Cellular componenti

  1. endosome Source: Ensembl
  2. membrane raft Source: Ensembl
  3. nucleus Source: UniProtKB-KW
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 537536Tyrosine-protein kinase FynPRO_0000282943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity
Lipidationi6 – 61S-palmitoyl cysteineBy similarity
Modified residuei12 – 121Phosphothreonine; by PKCBy similarity
Modified residuei15 – 151PhosphothreonineBy similarity
Modified residuei21 – 211PhosphoserineBy similarity
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei185 – 1851PhosphotyrosineBy similarity
Modified residuei213 – 2131PhosphotyrosineBy similarity
Modified residuei214 – 2141PhosphotyrosineBy similarity
Modified residuei257 – 2571PhosphoserineBy similarity
Modified residuei420 – 4201Phosphotyrosine; by autocatalysisBy similarity
Modified residuei440 – 4401PhosphotyrosineBy similarity
Modified residuei512 – 5121PhosphothreonineBy similarity
Modified residuei531 – 5311Phosphotyrosine; by CSKBy similarity

Post-translational modificationi

Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling (By similarity).By similarity
Palmitoylation at Cys-3 and Cys-6 regulate subcellular location.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Interactioni

Subunit structurei

Interacts (via its SH3 domain) with PIK3R1 and PRMT8. Interacts with FYB, PAG1, and SH2D1A. Interacts with CD79A (tyrosine-phosphorylated form); the interaction increases FYN activity. Interacts with TOM1L1 (phosphorylated form). Interacts with SH2D1A and SLAMF1. Interacts with and phosphorylates ITCH, down-regulating its activity. Interacts with FASLG. Interacts with RUNX3. Interacts with KIT. Interacts with KDR (tyrosine phosphorylated). Interacts with EPHA8; possible downstream effector of EPHA8 in regulation of cell adhesion. Interacts with PTK2/FAK1; this interaction leads to PTK2/FAK1 phosphorylation and activation. Interacts with CAV1; this interaction couples integrins to the Ras-ERK pathway. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts (via SH3 domain) with KLHL2 (via N-terminus) (By similarity). Interacts with UNC119 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliA1Y2K1.
SMRiA1Y2K1. Positions 81-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 14362SH3PROSITE-ProRule annotationAdd
BLAST
Domaini149 – 24698SH2PROSITE-ProRule annotationAdd
BLAST
Domaini271 – 524254Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118938.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiA1Y2K1.
KOiK05703.
OMAiSHNSGYR.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1Y2K1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGCVQCKDKE ATKLTEERDG SLNQSSGFRY GTDPTPQHYP SFGVTSIPNY
60 70 80 90 100
NNFHAAGGQG LTVFGGVSSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD
110 120 130 140 150
LSFHKGEKFQ ILNSSEGDWW EARSLTTGET GYIPSNYVAP VDSIQAEEWY
160 170 180 190 200
FGKLGRKDAE RQLLSFGNPR GTFLIRESET TKGAYSLSIR DWDDMKGDHV
210 220 230 240 250
KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC RLVVPCHKGM
260 270 280 290 300
PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT
310 320 330 340 350
LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSL
360 370 380 390 400
LDFLKDGEGR ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN
410 420 430 440 450
GLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW
460 470 480 490 500
SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPISLHELMI
510 520 530
HCWKKDPEER PTFEYLQGFL EDYFTATEPQ YQPGENL
Length:537
Mass (Da):60,689
Last modified:February 20, 2007 - v2
Checksum:i1CD6E4665939438F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ836055 mRNA. Translation: ABI33874.2.
RefSeqiNP_001073675.2. NM_001080206.2.
UniGeneiSsc.44857.

Genome annotation databases

EnsembliENSSSCT00000004884; ENSSSCP00000004769; ENSSSCG00000004421.
GeneIDi791125.
KEGGissc:791125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ836055 mRNA. Translation: ABI33874.2 .
RefSeqi NP_001073675.2. NM_001080206.2.
UniGenei Ssc.44857.

3D structure databases

ProteinModelPortali A1Y2K1.
SMRi A1Y2K1. Positions 81-537.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000004884 ; ENSSSCP00000004769 ; ENSSSCG00000004421 .
GeneIDi 791125.
KEGGi ssc:791125.

Organism-specific databases

CTDi 2534.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118938.
HOGENOMi HOG000233858.
HOVERGENi HBG008761.
InParanoidi A1Y2K1.
KOi K05703.
OMAi SHNSGYR.
TreeFami TF351634.

Enzyme and pathway databases

Reactomei REACT_192916. Signaling by SCF-KIT.
REACT_193226. DAP12 signaling.
REACT_202079. Sema3A PAK dependent Axon repulsion.
REACT_209088. CD28 dependent PI3K/Akt signaling.
REACT_210728. Regulation of KIT signaling.
REACT_210856. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.
REACT_212538. Role of LAT2/NTAL/LAB on calcium mobilization.
REACT_213066. Nephrin interactions.
REACT_214206. CTLA4 inhibitory signaling.
REACT_214362. Regulation of signaling by CBL.
REACT_217307. Netrin mediated repulsion signals.
REACT_218737. CRMPs in Sema3A signaling.
REACT_219289. PIP3 activates AKT signaling.
REACT_223200. Platelet Adhesion to exposed collagen.
REACT_223707. CD28 co-stimulation.
REACT_224799. Constitutive PI3K/AKT Signaling in Cancer.
REACT_226043. DCC mediated attractive signaling.
REACT_226779. CD28 dependent Vav1 pathway.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of porcine FYN gene."
    Long H., Yang Z.
    Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiFYN_PIG
AccessioniPrimary (citable) accession number: A1Y2K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: February 20, 2007
Last modified: October 29, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3