A1XSY8 (EGR2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 SUMO-protein ligase EGR2
EC=6.3.2.-

Alternative name(s):
Early growth response protein 2
Short name=EGR-2

Gene names

Name:

EGR2

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. Binds to the promoter region of ERBB2 By similarity. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity By similarity.
Pathway	Protein modification; protein sumoylation.
Subunit structure	Interacts with HCFC1. Interacts with WWP2. Interacts with CITED1. Interacts (via phosphorylated form) with SFN. Interacts with UBC9 By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Ubiquitinated by WWP2 leading to proteasomal degradation By similarity.
Sequence similarities	Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Ligase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Schwann cell differentiation Inferred from electronic annotation. Source: Compara brain segmentation Inferred from electronic annotation. Source: Compara cellular response to organic substance Inferred from electronic annotation. Source: Compara facial nerve structural organization Inferred from electronic annotation. Source: Compara fat cell differentiation Inferred from electronic annotation. Source: Compara motor neuron axon guidance Inferred from electronic annotation. Source: Compara myelination Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB protein export from nucleus Inferred from sequence or structural similarity. Source: UniProtKB protein sumoylation Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of ossification Inferred from electronic annotation. Source: Compara rhombomere 3 formation Inferred from electronic annotation. Source: Compara rhombomere 5 formation Inferred from electronic annotation. Source: Compara rhythmic behavior Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB ligase activity Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB transcription regulatory region DNA binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	E3 SUMO-protein ligase EGR2		PRO_0000296388
Regions
	Zinc finger	335 – 359	25	C2H2-type 1
	Zinc finger	365 – 387	23	C2H2-type 2
	Zinc finger	393 – 415	23	C2H2-type 3
	Compositional bias	167 – 338	172	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	A1XSY8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 9C073404B9EB86A0	FASTA	471	49,911
	10 20 30 40 50 60 MMTAKAVDKI PVTLSGFVHQ LSDNIYPVED LAATSVTIFP NAELGSPFDQ MNGVAGDGMI 70 80 90 100 110 120 NIDMTGEKRS LDLPYPSSFA PVSAPRNQTF TYMGKFSIDP QYPGASCYPE GIINIVSAGI 130 140 150 160 170 180 LQGVTSPAST TASSNVTSAS PNPLATGPLG VCTMSQTQPD LDHLYSPPPP PPYSGCAGDL 190 200 210 220 230 240 YQDPSAFLSA ATTSTSSSLA YPPPPSYPSP KPATDPGLFP MIPDYPGFFP SQCQRDLHGT 250 260 270 280 290 300 AGPDRKPFPC PLDSLRVPPP LTPLSTIRNF TLGGPSAGTT GPGASGGSEG PRLPGSSAAA 310 320 330 340 350 360 AAAAYNPHHL PLRPILRPRK YPNRPSKTPV HERPYPCPAE GCDRRFSRSD ELTRHIRIHT 370 380 390 400 410 420 GHKPFQCRIC MRNFSRSDHL TTHIRTHTGE KPFACDYCGR KFARSDERKR HTKIHLRQKE 430 440 450 460 470 RKSSAPSSSV PAASTASCTG GAQPGGPLCS SNSSTIGGGS LGPCSSRTRT P

References

[1]	"Cloning, chromosome mapping and expression characteristics of porcine KLF4, KLF5, KLF7 and EGR2." Yang H.W., Yang Z.Q. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ665829 mRNA. Translation: ABG49107.1.
RefSeq	NP_001090957.1. NM_001097488.1.
UniGene	Ssc.51140.
3D structure databases
ProteinModelPortal	A1XSY8.
SMR	A1XSY8. Positions 332-418.
ModBase	Search...
Protein-protein interaction databases
STRING	9823.ENSSSCP00000010899.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000011190; ENSSSCP00000010899; ENSSSCG00000010224.
GeneID	100038004.
KEGG	ssc:100038004.
Organism-specific databases
CTD	1959.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00550000074455.
HOGENOM	HOG000036856.
HOVERGEN	HBG003909.
KO	K12496.
OMA	GALCSSN.
OrthoDB	EOG41NTMJ.
Enzyme and pathway databases
UniPathway	UPA00886.
Gene expression databases
ArrayExpress	A1XSY8.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR021849. DUF3446. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF11928. DUF3446. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EGR2_PIG

Accession

Primary (citable) accession number: A1XSY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	February 6, 2007
Last modified:	April 3, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program