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A1WY38

- SYI_HALHL

UniProt

A1WY38 - SYI_HALHL

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
    Binding sitei607 – 6071ATPUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation
    Metal bindingi927 – 9271ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHHAL349124:GI3I-1883-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Hhal_1836
    OrganismiHalorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1))
    Taxonomic identifieri349124 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeHalorhodospira
    ProteomesiUP000000647: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 941941Isoleucine--tRNA ligasePRO_1000022076Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi349124.Hhal_1836.

    Structurei

    3D structure databases

    ProteinModelPortaliA1WY38.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1WY38-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEYKHTLNL PHTEFPMRAR LAEREPQRLQ RWEEEDLYGA IRRARAGRER    50
    FILHDGPPYA NGDIHIGHAV NKILKDIIVK ARTLDGYDAP YIPGWDCHGL 100
    PIEHKVEEQA GKPGAELTYA QFRERCRAFA AEQVEGQRQD FKRLGVLGDW 150
    ERPYLTMDYA TEAGILRALA RIFEGGHVTQ GFKPVHWCAD CGSALAEAEV 200
    EYEERTSPAV DVRFAVVDEL ALQQRVLLEG EGVQAGTASV VIWTTTPWTL 250
    PANRAVAVHP ELEYVVVALD HSERLVLAAE LLEATLQRAG VECYEVVGRC 300
    RGADLEGLSL QHPFLDRQVP VVLGEHVTTD GGTGCVHTAP GHGQEDFEVG 350
    QCYGLEVTNP VDGAGCFFED TEHFAGLNVF DANPRVVEVL ESRGALFHHE 400
    KYRHSYPHCW RHKTPVIFRA TPQWFIDLDR HGMRECALAG IEGVRWMPDW 450
    GQARIDAMVR GRPDWCISRQ RHWGVPIAVF IHRRSGEPHP QTPQHMEAVA 500
    ARMEHEGLEA WWDLDPAELL GDEAAEYEKV TDILDVWFDS GVTHATVLEQ 550
    REGLQVPADL YLEGSDQHRG WFQSSLLSSA AIRQAAPYRG VLTHGFTVDE 600
    QGHKMSKSRG NVVAPQDVMD RLGADILRLW VASADYSGEI AVSDNILQRT 650
    ADAYRRMRNT ARFLLGNLHG FEPGRDALVA EQLLPLDRWA VARTRALQER 700
    IVAAYDRYEL HRIYHLLHNF CVVDMGGFYL DVLKDRLYTT PADSRARRSG 750
    QTAMYHIAEA LVRWLAPILS FTADEIWGHL PGERREPVFT AEWYDGLFPL 800
    DDEPAEAAFW DRVMEVRTAV SRELERLRNE KVIGANLDAE VDLYVSSALA 850
    EELAPLGDEL RFVLITSAAR IHAATEAPVD AASATLEDGS EVRIAVAASA 900
    HDKCPRCWHR SPDIGASDEH PELCGRCVEN VAGSGEYRAT A 941
    Length:941
    Mass (Da):105,606
    Last modified:February 6, 2007 - v1
    Checksum:i582112F48B8FB30E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000544 Genomic DNA. Translation: ABM62600.1.
    RefSeqiWP_011814622.1. NC_008789.1.
    YP_001003402.1. NC_008789.1.

    Genome annotation databases

    EnsemblBacteriaiABM62600; ABM62600; Hhal_1836.
    GeneIDi4711401.
    KEGGihha:Hhal_1836.
    PATRICi22097959. VBIHalHal112047_1809.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000544 Genomic DNA. Translation: ABM62600.1 .
    RefSeqi WP_011814622.1. NC_008789.1.
    YP_001003402.1. NC_008789.1.

    3D structure databases

    ProteinModelPortali A1WY38.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349124.Hhal_1836.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM62600 ; ABM62600 ; Hhal_1836 .
    GeneIDi 4711401.
    KEGGi hha:Hhal_1836.
    PATRICi 22097959. VBIHalHal112047_1809.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HHAL349124:GI3I-1883-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 244 / SL1.

    Entry informationi

    Entry nameiSYI_HALHL
    AccessioniPrimary (citable) accession number: A1WY38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3