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A1WY38

- SYI_HALHL

UniProt

A1WY38 - SYI_HALHL

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei607 – 6071ATPUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation
Metal bindingi927 – 9271ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHHAL349124:GI3I-1883-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Hhal_1836
OrganismiHalorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1))
Taxonomic identifieri349124 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeHalorhodospira
ProteomesiUP000000647: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Isoleucine--tRNA ligasePRO_1000022076Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349124.Hhal_1836.

Structurei

3D structure databases

ProteinModelPortaliA1WY38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi604 – 6085"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1WY38-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEYKHTLNL PHTEFPMRAR LAEREPQRLQ RWEEEDLYGA IRRARAGRER
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDIIVK ARTLDGYDAP YIPGWDCHGL
110 120 130 140 150
PIEHKVEEQA GKPGAELTYA QFRERCRAFA AEQVEGQRQD FKRLGVLGDW
160 170 180 190 200
ERPYLTMDYA TEAGILRALA RIFEGGHVTQ GFKPVHWCAD CGSALAEAEV
210 220 230 240 250
EYEERTSPAV DVRFAVVDEL ALQQRVLLEG EGVQAGTASV VIWTTTPWTL
260 270 280 290 300
PANRAVAVHP ELEYVVVALD HSERLVLAAE LLEATLQRAG VECYEVVGRC
310 320 330 340 350
RGADLEGLSL QHPFLDRQVP VVLGEHVTTD GGTGCVHTAP GHGQEDFEVG
360 370 380 390 400
QCYGLEVTNP VDGAGCFFED TEHFAGLNVF DANPRVVEVL ESRGALFHHE
410 420 430 440 450
KYRHSYPHCW RHKTPVIFRA TPQWFIDLDR HGMRECALAG IEGVRWMPDW
460 470 480 490 500
GQARIDAMVR GRPDWCISRQ RHWGVPIAVF IHRRSGEPHP QTPQHMEAVA
510 520 530 540 550
ARMEHEGLEA WWDLDPAELL GDEAAEYEKV TDILDVWFDS GVTHATVLEQ
560 570 580 590 600
REGLQVPADL YLEGSDQHRG WFQSSLLSSA AIRQAAPYRG VLTHGFTVDE
610 620 630 640 650
QGHKMSKSRG NVVAPQDVMD RLGADILRLW VASADYSGEI AVSDNILQRT
660 670 680 690 700
ADAYRRMRNT ARFLLGNLHG FEPGRDALVA EQLLPLDRWA VARTRALQER
710 720 730 740 750
IVAAYDRYEL HRIYHLLHNF CVVDMGGFYL DVLKDRLYTT PADSRARRSG
760 770 780 790 800
QTAMYHIAEA LVRWLAPILS FTADEIWGHL PGERREPVFT AEWYDGLFPL
810 820 830 840 850
DDEPAEAAFW DRVMEVRTAV SRELERLRNE KVIGANLDAE VDLYVSSALA
860 870 880 890 900
EELAPLGDEL RFVLITSAAR IHAATEAPVD AASATLEDGS EVRIAVAASA
910 920 930 940
HDKCPRCWHR SPDIGASDEH PELCGRCVEN VAGSGEYRAT A
Length:941
Mass (Da):105,606
Last modified:February 6, 2007 - v1
Checksum:i582112F48B8FB30E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000544 Genomic DNA. Translation: ABM62600.1.
RefSeqiYP_001003402.1. NC_008789.1.

Genome annotation databases

EnsemblBacteriaiABM62600; ABM62600; Hhal_1836.
GeneIDi4711401.
KEGGihha:Hhal_1836.
PATRICi22097959. VBIHalHal112047_1809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000544 Genomic DNA. Translation: ABM62600.1 .
RefSeqi YP_001003402.1. NC_008789.1.

3D structure databases

ProteinModelPortali A1WY38.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349124.Hhal_1836.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM62600 ; ABM62600 ; Hhal_1836 .
GeneIDi 4711401.
KEGGi hha:Hhal_1836.
PATRICi 22097959. VBIHalHal112047_1809.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HHAL349124:GI3I-1883-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 244 / SL1.

Entry informationi

Entry nameiSYI_HALHL
AccessioniPrimary (citable) accession number: A1WY38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3