Reviewed, UniProtKB/Swiss-Prot A1WXM7 (SAHH_HALHL)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	Hhal_1675

Organism

Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1)) [Complete proteome] [HAMAP]

Taxonomic identifier

349124 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Halorhodospira

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000061125
Regions
	Region	183 – 348	166	NAD binding By similarity
Sites
	Binding site	56	1	Substrate By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WXM7-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 1B0D75461642D506	FASTA	430	47,510
	10 20 30 40 50 60 MSNQDYKVAD ISLADWGRKE IKIAESEMPG LMETRREFAA QKPLKGARIA GCLHMTIQTA 70 80 90 100 110 120 VLIETLQELG AEVRWSSCNI FSTQDQAAAA VAANGTPVFA WKGETEEEYW WCIEQTINGP 130 140 150 160 170 180 DGWKPNMLLD DGGDLTAVIH EQYPDMMKDI YGVSEETTTG VHRLYEMSRK GELGMPAFNV 190 200 210 220 230 240 NDSVTKSKFD NLYGCRESLV DSIKRATDVM IAGKVAVVAG FGDVGKGSAQ SLRGLGAQVW 250 260 270 280 290 300 VTEVDPICAL QASMDGYKVV TMEEAAPVAD IFVTATGNYN VITHDHMKAM KDEAIVCNIG 310 320 330 340 350 360 HFDNEIDVAS LKQYKWDEIK PQVDHVEFPD GKKITLLAEG RLVNLGCATG HPSFVMSNSF 370 380 390 400 410 420 TNQVMAQMEL YNNPGKYEKD VYVLPKHLDE KVAALHLGRV GANLTRLSEE QAGYIGVSVD 430 GPFKPEWYRY

References

[1]

"Complete sequence of Halorhodospira halophila SL1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Hoff W., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000544 Genomic DNA. Translation: ABM62439.1.
RefSeq	YP_001003241.1.
3D structure databases
SMR	A1WXM7. Positions 4-430.
ModBase	Search...
Protein-protein interaction databases
STRING	A1WXM7.
Genome annotation databases
GeneID	4709919.
GenomeReviews	Gene locus Hhal_1675 in contig CP000544_GR.
KEGG	hha:Hhal_1675.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0499.
HOGENOM	HBG352029.
OMA	VITHDHM.
PhylomeDB	A1WXM7.
Family and domain databases
HAMAP	MF_00563_B. AdoHcyase_B. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_HALHL

Accession

Primary (citable) accession number: A1WXM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)