A1WVR6 (GATB_HALHL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	Hhal_1002

Organism

Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1)) [Complete proteome] [HAMAP]

Taxonomic identifier

349124 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Halorhodospira › Halorhodospira halophila

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_1000015972

Sequences

	Sequence		Length	Mass (Da)
	A1WVR6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 7E147F88B89920FB	FASTA	479	53,120
	10 20 30 40 50 60 MKWETVIGLE IHAQLATRTK IFSGAPTAYG AEPNTQACPV DLGLPGVLPV LNREVVRMAI 70 80 90 100 110 120 KFGLAVDARI APRSVFARKN YFYPDLPKGY QISQYDLPIV EGGHLDIELE DGASKRIGIT 130 140 150 160 170 180 RAHLEEDAGK SLHEDFHGMT GVDLNRAGTP LMEIVSEPDL RSPAEAAAYM KKLHALVRYL 190 200 210 220 230 240 EICDGNMQEG SFRCDANVSV RPVGQEAFGT RAELKNLNSF RFVERALEYE VERQIDLLES 250 260 270 280 290 300 GGEVVQETRL YDVDKGVTRS MRTKEEANDY RYFPEPDLLP VEVDAALVDE VRETLPELPD 310 320 330 340 350 360 EKRRRFEEEY GLPAYDAGVL TATRDMADFF EHAVSESGGF AKRTANLLMS ELLAYLNKDG 370 380 390 400 410 420 LEIAESPVTP EMLGKLVARV EDETLSSRGA KDVFEAMWAG EGEPDEVIEK KGLKQVTDTS 430 440 450 460 470 AIEALVDEAI ANNPQQLEQY RAGKEKLFGF FVGQVMKASG GKANPQQVNE LLKKKLDGS

References

[1]

"Complete sequence of Halorhodospira halophila SL1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Hoff W., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 244 / SL1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000544 Genomic DNA. Translation: ABM61778.1.
RefSeq	YP_001002580.1. NC_008789.1.
3D structure databases
ProteinModelPortal	A1WVR6.
SMR	A1WVR6. Positions 2-403.
ModBase	Search...
Protein-protein interaction databases
STRING	349124.Hhal_1002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM61778; ABM61778; Hhal_1002.
GeneID	4709576.
KEGG	hha:Hhal_1002.
PATRIC	22096311. VBIHalHal112047_0997.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223743.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	HHAL349124:GI3I-1056-MONOMER.
Family and domain databases
Gene3D	1.10.10.410. 1 hit.
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_HALHL

Accession

Primary (citable) accession number: A1WVR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program