Reviewed, UniProtKB/Swiss-Prot A1WV00 (PYRC_HALHL)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Hhal_0730

Organism

Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1)) [Complete proteome] [HAMAP]

Taxonomic identifier

349124 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Halorhodospira

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6. HAMAP MF_00219
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Dihydroorotase HAMAP MF_00219		PRO_0000325571
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	247	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WV00-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 8585E702D86298A1	FASTA	345	37,158
	10 20 30 40 50 60 MDTLTLPRPD DWHLHLRDGA LMQAVAPQAA AVFSRAIIMP NLQPPVTTVE QAAAYRQRIL 70 80 90 100 110 120 GALPAGSTFE PLMTLYLTDK TSPEEIVRAA ASGFVHAVKL YPAGATTHSD AGVTDLLLCY 130 140 150 160 170 180 DALAAMEEQG LPLLIHGERT GPELDIFERE TAFIDDVLQP LLDRHPRLKV VLEHVTTAVG 190 200 210 220 230 240 AELVTGDDPR LAGTFTPQHL LYNRNDLLVG GLKPHYYCMP ILKREADREA LLEAATSGHP 250 260 270 280 290 300 RVFLGTDSAP HPRGAKESAC GCAGCYTAPI ALSLYAEAFE AAGALERLAD FAGRNGPAFY 310 320 330 340 GLPVNPETVT LERAPLEVPA SYPGGDEPVV PLRAGESLAW RLHQP

References

[1]

"Complete sequence of Halorhodospira halophila SL1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Hoff W., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000544 Genomic DNA. Translation: ABM61512.1.
RefSeq	YP_001002314.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4711119.
GenomeReviews	Gene locus Hhal_0730 in contig CP000544_GR.
KEGG	hha:Hhal_0730.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1WV00. IMPNLVP.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_HALHL

Accession

Primary (citable) accession number: A1WV00

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)