A1WUH1 (KATG_HALHL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Hhal_0547

Organism

Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1)) [Complete proteome] [HAMAP]

Taxonomic identifier

349124 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Halorhodospira

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Catalase-peroxidase HAMAP MF_01961		PRO_0000354807
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WUH1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3EADD6749BEC678D	FASTA	724	80,764
	10 20 30 40 50 60 MADQQKAGGC PVMHGAMTQV GESNLDMWPN ALNLDILHQH DRKPDPMGEG FNYREEVKKL 70 80 90 100 110 120 DLDAVKQDLH QLMTDSQAWW PADWGHYGGL MIRMAWHAAG TYRVADGRGG GGTGNQRFAP 130 140 150 160 170 180 INSWPDNVNL DKARRLLWPI KKKYGNRLSW ADLIILAGNV AYESMGLKTF GFSLGREDIW 190 200 210 220 230 240 HPEKDIYWGS EKEWLAPSDS EDSRYGEDRA SLENPLAAVM MGLIYVNPEG VDGNPDPLRT 250 260 270 280 290 300 AEDVRITFER MAMNDEETVA LTAGGHTVGK CHGNGDVENL GPDPESADVE EQGLGWNNKV 310 320 330 340 350 360 TRGVGRDTVS SGIEGAWTTY PTRWDNGYFH LLLNYEWELT KSPAGAWQWE PVDIKEEDKP 370 380 390 400 410 420 VDVEDPSIRL NPIMTDADMA MKMDPAYRKI SERFYNDPAY FDEVFARAWF KLTHRDLGPR 430 440 450 460 470 480 TRYIGPEAPQ EDLIWQDPVP AGRTDYDVEA LKAKIADSGL SIGEMVSTAW DSARTFRGSD 490 500 510 520 530 540 NRGGANGARI RLAPQKDWEG NEPERLSKVL GVLEGIAADA GASLADTIVL AGNVGIEQAA 550 560 570 580 590 600 RAAGHDITVP FAPGRGDASQ EMTDVDSFQY LEPLADGYRN WVKKEYAVQP EEMMLDRTQL 610 620 630 640 650 660 MGLTAPEMTV LVGGMRVLGT NHGGTKHGVL TDREGQLTND FFVNLTDMAY TWKPVGSNRY 670 680 690 700 710 720 EIRQRSSDAV KWTATRVDLV FGSNSILRSY AEVYAQDDNR EKFVHDFVAA WTKVMNADRF DLVA

References

[1]

"Complete sequence of Halorhodospira halophila SL1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Hoff W., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 244 / SL1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000544 Genomic DNA. Translation: ABM61333.1.
RefSeq	YP_001002135.1. NC_008789.1.
3D structure databases
ProteinModelPortal	A1WUH1.
SMR	A1WUH1. Positions 23-722.
ModBase	Search...
Protein-protein interaction databases
STRING	A1WUH1.
Protein family/group databases
PeroxiBase	3561. HhCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4709731.
GenomeReviews	Gene locus Hhal_0547 in contig CP000544_GR.
KEGG	hha:Hhal_0547.
PATRIC	22095383. VBIHalHal112047_0556.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	A1WUH1.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	HHAL349124:HHAL_0547-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_HALHL

Accession

Primary (citable) accession number: A1WUH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program