A1WT90 (SYE1_HALHL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Hhal_0108

Organism

Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1)) [Complete proteome] [HAMAP]

Taxonomic identifier

349124 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Halorhodospira

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367678
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	253 – 257	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	256	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WT90 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: EE16D0DD22492B03	FASTA	477	52,551
	10 20 30 40 50 60 MNEMTVKTRF APSPTGALHL GNLRTALFNA LLAYGHGGRF VLRIEDTDRE RYSPEATRSL 70 80 90 100 110 120 MGDLRWLGLD WQEGPEVGGP APPYHQFERG FLYDAYYRQL EAEGWAYPCF CSERELEMAR 130 140 150 160 170 180 RAQRSAGQPP RYPGTCARLT AEEVARKRAE GMQPALRFRV PSGDEVRFED RIHGEQRFRT 190 200 210 220 230 240 DDIGDFIIRR ADGTAAYLFT NAVDDALMGI TDVLRGDDHL TNTPRQLLIL QALGLAAPRY 250 260 270 280 290 300 GHMGLITGND GAPLSKRNGS RSVGELARDG YLPEAMLNYL ARVGHTCESE ALLDLAGLAE 310 320 330 340 350 360 GFDPARISTA PSRFDPDHLR HWQEEAVHGS SVRRLRPWMG IDALVPPGYA EDLVNAVRGN 370 380 390 400 410 420 VRFPAEARDW AKRVFAGPPE LDADAEAAIV SAGAEFFEAA AGAAEEGYTR FKGLTEAVKE 430 440 450 460 470 RTGVKGKQLF MPLRAALTGR CSGPELQPVV DLMGGERVVE RLRRAATLAA EQGAQGE

References

[1]

"Complete sequence of Halorhodospira halophila SL1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Hoff W., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 244 / SL1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000544 Genomic DNA. Translation: ABM60902.1.
RefSeq	YP_001001704.1. NC_008789.1.
3D structure databases
ProteinModelPortal	A1WT90.
ModBase	Search...
Protein-protein interaction databases
STRING	A1WT90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4710585.
GenomeReviews	Gene locus Hhal_0108 in contig CP000544_GR.
KEGG	hha:Hhal_0108.
PATRIC	22094487. VBIHalHal112047_0116.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	WQEGPEV.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	HHAL349124:HHAL_0108-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_HALHL

Accession

Primary (citable) accession number: A1WT90

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program