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A1WRL3 (DDL_VEREI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Veis_4572
OrganismVerminephrobacter eiseniae (strain EF01-2) [Complete proteome] [HAMAP]
Taxonomic identifier391735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVerminephrobacter

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341192

Regions

Domain112 – 314203ATP-grasp
Nucleotide binding138 – 19356ATP By similarity

Sites

Metal binding2671Magnesium or manganese 1 By similarity
Metal binding2811Magnesium or manganese 1 By similarity
Metal binding2811Magnesium or manganese 2 By similarity
Metal binding2831Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1WRL3 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 2EBB3D728D0CEB17

FASTA33135,110
        10         20         30         40         50         60 
MSLFDPHLDG ASLGKVAVLM GGVSAEREVS LLSGAGVLRA LRARAVDAHA FDTAQGDLGA 

        70         80         90        100        110        120 
LKREGYARCF IALHGRHGED GTVQGALELL GIAYTGSGVM ASSMALDKTM SKRIWRSEGL 

       130        140        150        160        170        180 
PTPDWRLVTS GAEAGQALQT LGAPMIVKPA REGSTIGLSK VHQAQQCASA YLLAARYDPE 

       190        200        210        220        230        240 
VLCEQFIAGD ELTCTVLDQG RRASAQALPL IRIVAPDGNY DYQHKYFSDA TRYHCPSGLP 

       250        260        270        280        290        300 
EAQERAIGRL AEQAFSALGC RGWARADIML RASDQQPFLL EINTAPGMTD HSLVPMSARA 

       310        320        330 
AGISYEDLCL RLLAMATLDT PPGALSGAAR A 

« Hide

References

[1]"Complete sequence of chromosome 1 of Verminephrobacter eiseniae EF01-2."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EF01-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000542 Genomic DNA. Translation: ABM60270.1.
RefSeqYP_999288.1. NC_008786.1.

3D structure databases

ProteinModelPortalA1WRL3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391735.Veis_4572.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM60270; ABM60270; Veis_4572.
GeneID4691354.
KEGGvei:Veis_4572.
PATRIC24023085. VBIVerEis120356_4913.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycVEIS391735:GHY5-4625-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_VEREI
AccessionPrimary (citable) accession number: A1WRL3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways