A1WMW1 (RNC_VEREI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	Veis_3238

Organism

Verminephrobacter eiseniae (strain EF01-2) [Complete proteome] [HAMAP]

Taxonomic identifier

391735 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Verminephrobacter › Verminephrobacter eiseniae

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000075847
Regions
	Domain	5 – 147	143	RNase III
	Domain	174 – 244	71	DRBM
Sites
	Active site	44	1	Potential
	Active site	136	1	By similarity
	Metal binding	40	1	Magnesium By similarity
	Metal binding	133	1	Magnesium By similarity
	Metal binding	136	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WMW1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 1BAB696E9AB98D5D	FASTA	247	26,871
	10 20 30 40 50 60 MRPDLIALQE RLQHVFSDPG LLERATTHRS FSAEHNERLE FLGDSVLNLA VSSLLYQRLS 70 80 90 100 110 120 ALPEGELSRV RANLVRQESL YQLALRLPLA QALRLGESLS GNRQRRSRRR CADELQPDEA 130 140 150 160 170 180 GSGGLQHPSI LADALEALIG AVYLDAGYAS AQALVQRLFQ DVEISPRMQA AARDAKTALQ 190 200 210 220 230 240 EWLASRKMKP PQYQVVARVG AAHCPTFEVA CGIPALGLTE RGSAGSRQAG EQAAAAAMLA TLKARNL

References

[1]

"Complete sequence of chromosome 1 of Verminephrobacter eiseniae EF01-2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EF01-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000542 Genomic DNA. Translation: ABM58968.1.
RefSeq	YP_997986.1. NC_008786.1.
3D structure databases
ProteinModelPortal	A1WMW1.
ModBase	Search...
Protein-protein interaction databases
STRING	391735.Veis_3238.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM58968; ABM58968; Veis_3238.
GeneID	4690873.
KEGG	vei:Veis_3238.
PATRIC	24020255. VBIVerEis120356_3506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246809.
KO	K03685.
OMA	RACELPR.
ProtClustDB	PRK00102.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_VEREI

Accession

Primary (citable) accession number: A1WMW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program