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A1WIH0 (TRMB_VEREI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Veis_1672
OrganismVerminephrobacter eiseniae (strain EF01-2) [Complete proteome] [HAMAP]
Taxonomic identifier391735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeVerminephrobacter

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288248

Regions

Region252 – 2554Substrate binding By similarity

Sites

Active site1811 By similarity
Binding site1061S-adenosyl-L-methionine By similarity
Binding site1311S-adenosyl-L-methionine By similarity
Binding site1581S-adenosyl-L-methionine By similarity
Binding site1811S-adenosyl-L-methionine By similarity
Binding site1851Substrate By similarity
Binding site2171Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1WIH0 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: FA0B9199B1039C70

FASTA27429,763
        10         20         30         40         50         60 
MAPKLQWPIV TELTTASASR GAATGSRGVA PAVPRGGAPA DVAHPQTIKS FVRRAGRTTA 

        70         80         90        100        110        120 
AQARAFEDLG PRFVVPYAPI APIAPIAPAA PAAGAWFGRD APLVLEIGFG MGEATAHIAR 

       130        140        150        160        170        180 
VRPQDDFLCC EVHRPGVGAL LKRIAEHGLT NIRILEHDAV EVIDHMLPES CLAGVHIFFP 

       190        200        210        220        230        240 
DPWHKSRHHK RRLIQPPLVA RLAARLQAGG YLHCATDWQP YAEQMLQVLG AEPLLRNTAP 

       250        260        270 
GFAPKPGYRP LTKFENRGLR LGHGVRDLVF ERRH 

« Hide

References

[1]"Complete sequence of chromosome 1 of Verminephrobacter eiseniae EF01-2."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EF01-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000542 Genomic DNA. Translation: ABM57427.1.
RefSeqYP_996445.1. NC_008786.1.

3D structure databases

ProteinModelPortalA1WIH0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391735.Veis_1672.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM57427; ABM57427; Veis_1672.
GeneID4691171.
KEGGvei:Veis_1672.
PATRIC24016907. VBIVerEis120356_1852.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycVEIS391735:GHY5-1685-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_VEREI
AccessionPrimary (citable) accession number: A1WIH0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways