A1WHV2 (LPXD_VEREI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Veis_1448

Organism

Verminephrobacter eiseniae (strain EF01-2) [Complete proteome] [HAMAP]

Taxonomic identifier

391735 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Verminephrobacter

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000050964
Sites
	Active site	225	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1WHV2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 71D7FAA9D9298E5D	FASTA	326	33,859
	10 20 30 40 50 60 MSLLLGQIVD ALGGSLQGGS RDTPICRIAP LQAAGPGDLS VLSHPRYQQQ LAASRAACVI 70 80 90 100 110 120 VAPALRAAAL ARGACILAEQ PYAYFARATQ LWRQHHAPAP APGVHASAVL DPTAQVHPTA 130 140 150 160 170 180 SIGPLCILER GAQVGAGSRL QARVTVGADC RIGARCLLHA GVVVGADGFG FAPEDGQWIK 190 200 210 220 230 240 IEQLGAVRIG DDVEIGANTC IDRGTLQDTV IEDGVKLDNL IQIGHNVRIG KHSALAGCVG 250 260 270 280 290 300 VAGSARIGAH CTIGGGAIVL GHLELADHVH ISAATVVTRS LTRPGQYTGL FPIDDNARWE 310 320 KNAATLKQLH SLRERIKALE QALKAA

References

[1]

"Complete sequence of chromosome 1 of Verminephrobacter eiseniae EF01-2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EF01-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000542 Genomic DNA. Translation: ABM57209.1.
RefSeq	YP_996227.1. NC_008786.1.
3D structure databases
ProteinModelPortal	A1WHV2.
ModBase	Search...
Protein-protein interaction databases
STRING	A1WHV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4692680.
GenomeReviews	Gene locus Veis_1448 in contig CP000542_GR.
KEGG	vei:Veis_1448.
NMPDR	fig|391735.5.peg.1362.
PATRIC	24016401. VBIVerEis120356_1600.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	A1WHV2.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	VEIS391735:VEIS_1448-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 3 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_VEREI

Accession

Primary (citable) accession number: A1WHV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program